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Article ; Online: Study of compaction tools and parameters on critical quality attributes of high drug load minitablets.

Loo, Shang Jun / Lim, Cheng Yee / Heng, Paul Wan Sia / Chan, Lai Wah

2024 Volume 652, Page(s) 123806

Abstract: Minitablets are prepared using multiple die openings and multi-tip punches for greater productivity. With multiple tips on the punch barrel, the overall compaction force to be applied is commonly estimated by multiplying the desired compaction force per ... ...

Abstract	Minitablets are prepared using multiple die openings and multi-tip punches for greater productivity. With multiple tips on the punch barrel, the overall compaction force to be applied is commonly estimated by multiplying the desired compaction force per tip by the number of punch tips. Few researchers have however examined this proportionality and the effects of the number of punch tips and punch face geometry on the critical quality attributes (CQAs) of high drug load minitablets. In this study, the minitablets prepared by multi-tip tools exhibited greater weight variation than those prepared by single-tip tools. Their compaction was accompanied by a longer dwell time that led to a higher minitablet tensile strength and consequently a longer disintegration time. The compaction forces required to achieve a consistent set of minitablet CQAs were not directly proportional to the number of punch tips used. In comparison, the effect of punch face geometry was negligible. Increasing concentration of magnesium stearate (as lubricant) from 0.75 to 1.25 %, w/w reduced weight variation, especially of minitablets prepared by the multi-tip tools. It also increased the disintegration time but had no significant effect on the tensile strength of the minitablets regardless of type of tools used. The adjustment of compaction speed was an effective compensatory method to mitigate the differences in dwell time and tensile strength between minitablets prepared by single-tip and multi-tip standard concave tools. A larger reduction in compaction speed of the single-tip tools was required at higher compaction pressures.
MeSH term(s)	Tablets ; Excipients ; Tensile Strength ; Pressure ; Drug Compounding/methods
Chemical Substances	Tablets ; Excipients
Language	English
Publishing date	2024-01-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2024.123806
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Article ; Online: Comparison of efficacy between unilateral biportal endoscopic lumbar fusion versus minimally invasive transforaminal lumbar fusion in the treatment of lumbar degenerative diseases: A systematic review and meta-analysis.

Li, Yang / Gao, Shang Jun / Hu, Xu / Lin, Shi Shui

Medicine

2023 Volume 102, Issue 34, Page(s) e34705

Abstract: Background: To evaluate the clinical efficacy and prognosis of unilateral biportal endoscopic lumbar fusion (ULIF) and minimally invasive transforaminal lumbar fusion (MIS-TLIF) for lumbar degenerative diseases.: Methods: Chinese and English ... ...

Abstract	Background: To evaluate the clinical efficacy and prognosis of unilateral biportal endoscopic lumbar fusion (ULIF) and minimally invasive transforaminal lumbar fusion (MIS-TLIF) for lumbar degenerative diseases. Methods: Chinese and English databases were retrieved for the period from database creation to December 31, 2022. Case-control studies on unilateral biportal endoscopic lumbar fusion were collected. The observation indexes consisted of operation times, intraoperative blood loss, postoperative drainage volume, length of hospital stay, postoperative pain score, postoperative oswestry disability index score, postoperative MacNab excellent and good rate, imaging fusion rate at the last follow-up, and complications. The NO rating table was employed to assess the quality of the included literature, and a meta-analysis was conducted using Revman5.4.1 and Stata17. Results: Ten studies with 738 surgical patients were considered, including 347 patients in the ULIF group and 391 in the MIS-TLIF group. This Meta-analysis demonstrated statistically significant differences in mean operation duration, intraoperative blood loss, postoperative drainage volume, length of hospital stay, and early postoperative (1-2W) visual analogue scale/score (VAS) scores for back pain. No significant differences were observed in the final follow-up postoperative VAS scores for back pain, postoperative leg VAS score, postoperative oswestry disability index score, excellent and good rate of postoperative modified MacNab, imaging fusion rate, and complications. Conclusion: Compared with the MIS-TLIF group, the ULIF group had longer operation time, lower intraoperative blood loss and postoperative drainage volume, lower lumbar VAS score in the early postoperative period, and shorter hospital stay. ULIF is less invasive than traditional MIS-TLIF, making it a trustworthy surgical option for lumbar degenerative diseases with comparable fusion efficiency, superior MacNab rate, and complication rate.
MeSH term(s)	Humans ; Lumbar Vertebrae/surgery ; Minimally Invasive Surgical Procedures ; Spinal Fusion ; Endoscopy ; Blood Loss, Surgical ; Postoperative Hemorrhage
Language	English
Publishing date	2023-08-31
Publishing country	United States
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000034705
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Article ; Online: Charge reduction assisted production of diminutive fluid bed granules for high drug load minitablets.

Loo, Shang Jun / Heng, Paul Wan Sia / Chan, Lai Wah

International journal of pharmaceutics

2022 Volume 623, Page(s) 121965

Abstract: Micronized drug powders are generally unsuitable as tableting feed to produce minitablets due to their cohesivity and poor flow. The silicification of fine paracetamol powder ( ... ...

Abstract	Micronized drug powders are generally unsuitable as tableting feed to produce minitablets due to their cohesivity and poor flow. The silicification of fine paracetamol powder (PCM
MeSH term(s)	Acetaminophen ; Drug Compounding ; Particle Size ; Powders ; Silicon Dioxide/chemistry ; Tablets/chemistry
Chemical Substances	Powders ; Tablets ; Acetaminophen (362O9ITL9D) ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2022-06-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2022.121965
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Article ; Online: Automated and fast online method for simultaneously determining a broad spectrum of per- and polyfluoroalkyl substances in a small volume of cerebrospinal fluid

Shang, Jun / Gao, Ke / Chi, Bowen / Piao, Yingshi / Ma, Qiuying / Lü, Liping / Wang, Jiawei

Anal Bioanal Chem. 2023 Mar., v. 415, no. 6 p.1221-1233

2023

Abstract: Per- and polyfluoroalkyl substances (PFASs) are potentially neurotoxic compounds. Levels of PFASs in cerebrospinal fluid (CSF) could directly reflect their potential harm to the central nervous system. Because of the variety of PFASs and the rarity of ... ...

Abstract	Per- and polyfluoroalkyl substances (PFASs) are potentially neurotoxic compounds. Levels of PFASs in cerebrospinal fluid (CSF) could directly reflect their potential harm to the central nervous system. Because of the variety of PFASs and the rarity of CSF, there is an urgent need to establish a rapid online method to detect a broad spectrum of PFASs accurately and simultaneously by consuming a small amount of CSF. In this study, we developed a fast and automated method to analyze 52 PFASs in human CSF samples using online TurboFlow ultra-high-performance liquid chromatography–tandem mass spectrometry. Our method offered excellent matrix-matched standard curve linearity (correlation coefficient > 0.99), good limits of quantitation (MLOQs) (0.01 to 0.08 ng mL⁻¹), satisfactory accuracy (recoveries of 74.6%–119.1%) and precision (relative standard deviations of 1.4%–13.2%), small sample amount consumption (50 μL), and fast analysis time (18 min per sample) without complex sample pretreatment procedures. These are advantageous for the high throughput screening of PFASs in environmental epidemiology studies. Repeated freeze–thaw experiments showed that it was better to perform the analytical process soon as possible after sample collection. The established method was used to analyze PFASs in 60 people. Short-chain PFASs, perfluorobutanoic acid (PFBA), perfluoropentanoic acid (PFPeA), and novel PFASs [sodium 2-(N-ethylperfluorooctane-1-sulfonamido)ethyl phosphate (SAmPAP), perfluoroethylcyclohexanesulfonate (PFECHS), and perfluoro-3, 7-dimethyloctanoic acid (P37DMOA)] were reported in CSF for the first time. PFBA and PFPeA were detected in all samples with mean concentrations of 0.24 and 0.22 ng mL⁻¹, respectively. We also calculated the blood–brain barrier transmission efficiency of PFASs (RPFAS), and the mean RPFBA value was above 1, which indicated that PFBA might transfer from serum to CSF.
Keywords	automation ; blood serum ; blood-brain barrier ; central nervous system ; cerebrospinal fluid ; epidemiology ; freeze-thaw cycles ; humans ; liquid chromatography ; neurotoxicity ; people ; perfluoropentanoic acid ; phosphates ; sodium ; tandem mass spectrometry
Language	English
Dates of publication	2023-03
Size	p. 1221-1233.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ISSN	1618-2642
DOI	10.1007/s00216-022-04507-4
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Article ; Online: Effect of chemotherapy on sex hormone levels in perimenopause women with breast cancer

Shang-Jun Liu

Journal of Hainan Medical University, Vol 22, Iss 11, Pp 122-

2016 Volume 124

Abstract: Objective: To explore the effect of chemotherapy on the sex hormone levels in perimenopause women with breast cancer. Methods: A total of 90 perimenopause women after breast cancer surgery who were admitted in our hospital from November, 2014 to November, ...

Abstract	Objective: To explore the effect of chemotherapy on the sex hormone levels in perimenopause women with breast cancer. Methods: A total of 90 perimenopause women after breast cancer surgery who were admitted in our hospital from November, 2014 to November, 2015 were included in the study. Those whose PR and ER were positive served as the observation group and were given TAM. Those whose PR and ER were negative served as the control group and were not given TAM. The chemiluminescence method was used to determine the levels of LH, FSH, and E2. The Doppler ultrasound device was used to measure the endometrial thickness and intrauterine depth before and after menopause. After fractional curettage by hysteroscopy, the patients in the observation group were performed with pathological examinations. The pathological change of endometrium before and after menopause was observed. Results: LH and FSH levels in the observation group were significantly lower than those in the control group, while E2 level was significantly higher than that in the control group. The comparison of endometrial thickness and intrauterine depth before and after menopause was not statistically significant. The occurrence rate of endometrial polyps, endometrial carcinoma, simple hyperplasia, and atypical hyperplasia in patients before menopause was significantly less than that in patients after menopause. Conclusions: Due to be in a special endocrine environment, during TAM administration period for perimenopause women with breast cancer, a regular determination of sex hormone levels, adoption of B ultrasound and hysteroscope to detect the endometrial thickness and pathological change are required in order to guide the clinical medication.
Keywords	Chemotherapy ; TAM ; Perimenopause women ; Breast cancer ; Sex hormone level ; Medicine ; R
Subject code	610
Language	English
Publishing date	2016-06-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
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Article ; Online: NLRRC: A Novel Clustering Method of Jointing Non-Negative LRR and Random Walk Graph Regularized NMF for Single-Cell Type Identification.

Wang, Juan / Wang, Lin-Ping / Yuan, Sha-Sha / Li, Feng / Liu, Jin-Xing / Shang, Jun-Liang

IEEE journal of biomedical and health informatics

2023 Volume 27, Issue 10, Page(s) 5199–5209

Abstract: The development of single-cell RNA sequencing (scRNA-seq) technology has opened up a new perspective for us to study disease mechanisms at the single cell level. Cell clustering reveals the natural grouping of cells, which is a vital step in scRNA-seq ... ...

Abstract	The development of single-cell RNA sequencing (scRNA-seq) technology has opened up a new perspective for us to study disease mechanisms at the single cell level. Cell clustering reveals the natural grouping of cells, which is a vital step in scRNA-seq data analysis. However, the high noise and dropout of single-cell data pose numerous challenges to cell clustering. In this study, we propose a novel matrix factorization method named NLRRC for single-cell type identification. NLRRC joins non-negative low-rank representation (LRR) and random walk graph regularized NMF (RWNMFC) to accurately reveal the natural grouping of cells. Specifically, we find the lowest rank representation of single-cell samples by non-negative LRR to reduce the difficulty of analyzing high-dimensional samples and capture the global information of the samples. Meanwhile, by using random walk graph regularization (RWGR) and NMF, RWNMFC captures manifold structure and cluster information before generating a cluster allocation matrix. The cluster assignment matrix contains cluster labels, which can be used directly to get the clustering results. The performance of NLRRC is validated on simulated and real single-cell datasets. The results of the experiments illustrate that NLRRC has a significant advantage in single-cell type identification.
MeSH term(s)	Humans ; Algorithms ; Cluster Analysis ; Single-Cell Analysis ; Gene Expression Profiling/methods
Language	English
Publishing date	2023-10-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2695320-1
ISSN	2168-2208 ; 2168-2194
ISSN (online)	2168-2208
ISSN	2168-2194
DOI	10.1109/JBHI.2023.3299748
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Article ; Online: Joint L

Wang, Tai-Ge / Shang, Jun-Liang / Liu, Jin-Xing / Li, Feng / Yuan, Shasha / Wang, Juan

Computer methods in biomechanics and biomedical engineering

2023 Volume 27, Issue 4, Page(s) 498–511

Abstract: The development and widespread utilization of high-throughput sequencing technologies in biology has fueled the rapid growth of single-cell RNA sequencing (scRNA-seq) data over the past decade. The development of scRNA-seq technology has significantly ... ...

Abstract	The development and widespread utilization of high-throughput sequencing technologies in biology has fueled the rapid growth of single-cell RNA sequencing (scRNA-seq) data over the past decade. The development of scRNA-seq technology has significantly expanded researchers' understanding of cellular heterogeneity. Accurate cell type identification is the prerequisite for any research on heterogeneous cell populations. However, due to the high noise and high dimensionality of scRNA-seq data, improving the effectiveness of cell type identification remains a challenge. As an effective dimensionality reduction method, Principal Component Analysis (PCA) is an essential tool for visualizing high-dimensional scRNA-seq data and identifying cell subpopulations. However, traditional PCA has some defects when used in mining the nonlinear manifold structure of the data and usually suffers from over-density of principal components (PCs). Therefore, we present a novel method in this paper called joint
MeSH term(s)	Single-Cell Gene Expression Analysis ; Principal Component Analysis ; Single-Cell Analysis/methods ; Algorithms ; Cluster Analysis
Language	English
Publishing date	2023-03-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2071764-7
ISSN	1476-8259 ; 1025-5842
ISSN (online)	1476-8259
ISSN	1025-5842
DOI	10.1080/10255842.2023.2188106
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Article ; Online: Inhibitory effect of acarbose on tyrosinase: application of molecular dynamics integrating inhibition kinetics.

Xu, Jie-Hao / Lee, Jinhyuk / Yin, Shang-Jun / Wang, Wei / Park, Yong-Doo

Journal of biomolecular structure & dynamics

2023 Volume 42, Issue 1, Page(s) 314–325

Abstract: Due to its clinical and cosmetic applications, investigators have paid attention to tyrosinase (TYR) inhibitor development. In this study, a TYR inhibition study with acarbose was investigated to gain insights into the regulation of the catalytic ... ...

Abstract	Due to its clinical and cosmetic applications, investigators have paid attention to tyrosinase (TYR) inhibitor development. In this study, a TYR inhibition study with acarbose was investigated to gain insights into the regulation of the catalytic function. Biochemical assay results indicated that acarbose was turned to be an inhibitor of TYR in a reversible binding manner and probed as a distinctive mixed-type inhibitor via measurement of double-reciprocal kinetic (
MeSH term(s)	Molecular Dynamics Simulation ; Monophenol Monooxygenase/metabolism ; Acarbose/pharmacology ; Enzyme Inhibitors/chemistry ; Catalytic Domain ; Molecular Docking Simulation ; Kinetics
Chemical Substances	Monophenol Monooxygenase (EC 1.14.18.1) ; Acarbose (T58MSI464G) ; Enzyme Inhibitors
Language	English
Publishing date	2023-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	49157-3
ISSN	1538-0254 ; 0739-1102
ISSN (online)	1538-0254
ISSN	0739-1102
DOI	10.1080/07391102.2023.2192800
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Article ; Online: Automated and fast online method for simultaneously determining a broad spectrum of per- and polyfluoroalkyl substances in a small volume of cerebrospinal fluid.

Shang, Jun / Gao, Ke / Chi, Bowen / Piao, Yingshi / Ma, Qiuying / Lu, Liping / Wang, Jiawei

Analytical and bioanalytical chemistry

2023 Volume 415, Issue 6, Page(s) 1221–1233

Abstract	Per- and polyfluoroalkyl substances (PFASs) are potentially neurotoxic compounds. Levels of PFASs in cerebrospinal fluid (CSF) could directly reflect their potential harm to the central nervous system. Because of the variety of PFASs and the rarity of CSF, there is an urgent need to establish a rapid online method to detect a broad spectrum of PFASs accurately and simultaneously by consuming a small amount of CSF. In this study, we developed a fast and automated method to analyze 52 PFASs in human CSF samples using online TurboFlow ultra-high-performance liquid chromatography-tandem mass spectrometry. Our method offered excellent matrix-matched standard curve linearity (correlation coefficient > 0.99), good limits of quantitation (MLOQs) (0.01 to 0.08 ng mL
MeSH term(s)	Humans ; Tandem Mass Spectrometry/methods ; Fluorocarbons/analysis ; Chromatography, High Pressure Liquid/methods ; Water Pollutants, Chemical/analysis
Chemical Substances	perfluoropentanoic acid ; Fluorocarbons ; perfluorobutyric acid (375-22-4) ; Water Pollutants, Chemical
Language	English
Publishing date	2023-01-12
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-022-04507-4
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Article ; Online: Study of diminutive granules as feed powders for manufacturability of high drug load minitablets.

Loo, Shang Jun / Seah, Xin Yun / Heng, Paul Wan Sia / Chan, Lai Wah

International journal of pharmaceutics

2023 Volume 638, Page(s) 122922

Abstract: The maximal amount of drug contained in a minitablet is limited. To reduce the total number of minitablets in a single dose, high drug load minitablets can be prepared from high drug load feed powders by various pharmaceutical processing techniques. Few ... ...

Abstract	The maximal amount of drug contained in a minitablet is limited. To reduce the total number of minitablets in a single dose, high drug load minitablets can be prepared from high drug load feed powders by various pharmaceutical processing techniques. Few researchers have however examined the influence of pharmaceutical processing techniques on the properties of high drug load feed powders, and consequently the manufacturability of high drug load minitablets. In this study, silicification of the high drug load physical mix feed powders alone did not yield satisfactory quality attributes and compaction parameters to produce good quality minitablets. The abrasive nature of fumed silica increased ejection force and damage to the compaction tools. Granulation of fine paracetamol powder was crucial for the preparation of good quality high drug load minitablets. The diminutive granules had superior powder packing and flow properties for homogenous and consistent filling of the small die cavities when preparing minitablets. Compared to the physical mix feed powders for direct compression, the granules which possessed higher plasticity, lower rearrangement and elastic energies, yielded better quality minitablets with high tensile strength and rapid disintegration time. High shear granulation demonstrated greater process robustness than fluid bed granulation, with less discernment on the quality attributes of feed powder. It could proceed without fumed silica, with the high shear forces reducing interparticulate cohesivity. An in-depth understanding on the properties of high drug load feed powders with inherently poor compactability and poor flowability is important for the manufacturability of high drug load minitablets.
MeSH term(s)	Technology, Pharmaceutical/methods ; Powders ; Tablets ; Tensile Strength ; Silicon Dioxide ; Drug Compounding ; Particle Size
Chemical Substances	Powders ; Tablets ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2023-04-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2023.122922
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