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Article: The association between peer relationship and learning engagement among adolescents: The chain mediating roles of self-efficacy and academic resilience.

Shao, Yanhong / Kang, Shumin

2022 Volume 13, Page(s) 938756

Abstract: Previous studies have shown that peer relationship affects learning engagement. And learning engagement plays a vital role in promoting knowledge acquisition and production, enhancing adolescents' academic success. However, few studies have focused on ... ...

Abstract	Previous studies have shown that peer relationship affects learning engagement. And learning engagement plays a vital role in promoting knowledge acquisition and production, enhancing adolescents' academic success. However, few studies have focused on the mechanism between peer relationship and learning engagement. As such, based on Social Cognitive Theory, this study attempts to explore how peer relationship of adolescents is linked to learning engagement through the chain mediating roles of self-efficacy and academic resilience. The participants were 250 students who were selected
Language	English
Publishing date	2022-08-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2563826-9
ISSN	1664-1078
ISSN	1664-1078
DOI	10.3389/fpsyg.2022.938756
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Article ; Online: How peer relationships affect academic achievement among junior high school students: The chain mediating roles of learning motivation and learning engagement.

Shao, Yanhong / Kang, Shumin / Lu, Quan / Zhang, Chao / Li, Ruoxi

BMC psychology

2024 Volume 12, Issue 1, Page(s) 278

Abstract: Background: Despite the recognition of the impact of peer relationships, learning motivation, and learning engagement on academic achievement, there is still a gap in understanding the specific mechanisms through which peer relationships impact academic ...

Abstract	Background: Despite the recognition of the impact of peer relationships, learning motivation, and learning engagement on academic achievement, there is still a gap in understanding the specific mechanisms through which peer relationships impact academic achievement via learning motivation and learning engagement. Methods: This study aims to investigate how peer relationships affect junior high school students' academic achievement through the chain mediating roles of learning motivation and learning engagement, employing the self-system model of motivational development as the theoretical framework. In January 2024, 717 participants were selected from two middle schools in eastern China (mean age = 13.49 years, SD = 0.5). The data analysis in this study was performed using the structural equation model (SEM) in AMOS 24.0 and SPSS 24.0. Results: The results showed that peer relationships were directly and significantly related to junior high school students' academic achievement, and that peer relationships were indirectly and positively related to junior high school students' academic achievement via learning motivation and learning engagement respectively. The results also revealed a significant indirect and positive relationship between peer relationships and junior high school students' academic achievement, mediated by the sequential mediating roles of learning motivation and learning engagement. Moreover, the path "peer relationship→learning motivation→academic achievement" has the strongest indirect effect. Conclusion: For junior high school students to achieve academic success, the appropriate interventions should be implemented to improve peer relationships, learning motivation, and learning engagement.
MeSH term(s)	Humans ; Adolescent ; Motivation ; Male ; Female ; Peer Group ; Academic Success ; Students/psychology ; Students/statistics & numerical data ; Learning ; China ; Interpersonal Relations ; Schools
Language	English
Publishing date	2024-05-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2705921-2
ISSN	2050-7283 ; 2050-7283
ISSN (online)	2050-7283
ISSN	2050-7283
DOI	10.1186/s40359-024-01780-z
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Article ; Online: The Clinical Application and Accuracy Evaluation of Noninvasive Prenatal Testing for Common Trisomy and Sex Chromosome Aneuploidy.

Wang, Yichao / Shao, Yanhong / Yu, Juan

Discovery medicine

2023 Volume 35, Issue 176, Page(s) 353–360

Abstract: Background: Noninvasive prenatal testing (NIPT) has been widely adopted in prenatal examination for fetal chromosomal aneuploidy. The present study aimed to evaluate the clinical features of NIPT for both common trisomy and sex chromosome aneuploidy ( ... ...

Abstract	Background: Noninvasive prenatal testing (NIPT) has been widely adopted in prenatal examination for fetal chromosomal aneuploidy. The present study aimed to evaluate the clinical features of NIPT for both common trisomy and sex chromosome aneuploidy (SCA). Methods: A total of 24,164 pregnant women with NIPT testing from July 2020 to June 2022 were recruited at the Linping Maternity and Child Health Care Hospital. Results: Ninety cases showed high risk of trisomy 21/18/13 with karyotype results available, and the sensitivity, specificity, and positive predictive value (PPV) were 98.41%, 99.88% and 68.89%, respectively. The three most important reasons for screening were advanced maternal age (AMA, 28.06%), intermediate risk of prenatal screening (20.34%) and Multiple of medium (MoM) abnormality of prenatal screening (17.38%). High risk of NIPT results with Z-score ≥15 have a higher PPV when compared to those with 3 ≤ Z-score < 10, and 10 ≤ Z-score < 15. Meanwhile, 97 pregnant women received positive results for fetal sex chromosome aneuploidy (SCA) in NIPT. In addition, the rate for further diagnostics of SCA was 64.95% and the PPV of SCA was 50.79%. Conclusions: Our data show that NIPT has a promising future in prenatal screening for genetic abnormalities of the fetus, and that the accuracy of NIPT is closely related to Z-score.
MeSH term(s)	Child ; Female ; Pregnancy ; Humans ; Trisomy/diagnosis ; Trisomy/genetics ; Noninvasive Prenatal Testing ; Sex Chromosome Aberrations ; Prenatal Diagnosis/methods ; Aneuploidy ; Trisomy 13 Syndrome/diagnosis ; Trisomy 13 Syndrome/genetics ; Sex Chromosomes/genetics
Language	English
Publishing date	2023-06-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2415544-5
ISSN	1944-7930 ; 1944-7930
ISSN (online)	1944-7930
ISSN	1944-7930
DOI	10.24976/Discov.Med.202335176.36
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Article: Development of the reading literacy questionnaire for EFL learners at primary schools.

Li, Weilai / Kang, Shumin / Shao, Yanhong

Frontiers in psychology

2023 Volume 14, Page(s) 1154076

Abstract: Previous studies have indicated that there are a variety of factors influencing reading literacy assessment, including linguistic, cognitive, and affective factors, but little has been done on how to integrate these influential factors reasonably in a ... ...

Abstract	Previous studies have indicated that there are a variety of factors influencing reading literacy assessment, including linguistic, cognitive, and affective factors, but little has been done on how to integrate these influential factors reasonably in a reading literacy instrument. As such, the purpose of this study is to develop and validate an English Reading Literacy Questionnaire (ERLQ) for English as foreign language (EFL) learners at the elementary level. The ERLQ was designed and revised through three rounds of validation with a sample of 784 pupils (Grades 3-6) in six primary schools from six provinces in China. Validity and reliability tests of the questionnaire were conducted with item analysis, Exploratory Factor Analysis (EFA), Confirmatory Factor Analysis (CFA), reliability test, and the analysis of criterion validity in SPSS 26.0 and AMOS 23.0. Results indicated that the revised ERLQ had high internal consistency, ranging from 0.729 to 0.823. The criterion validity of the ERLQ was supported by significant correlations to the Chinese Students' English Rating Scale verified by the authoritative department, with a correlation coefficient of 0.871. The study shows that the revised questionnaire, with 3 dimensions of 14 items, has high reliability and validity, which can be used as an assessment instrument for the intended audience. It also suggests that modifications may be made for further use in other regions and countries, depending on the background information of the learners.
Language	English
Publishing date	2023-05-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2563826-9
ISSN	1664-1078
ISSN	1664-1078
DOI	10.3389/fpsyg.2023.1154076
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Article ; Online: Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑D‑neuraminic acid.

Zhang, Qiong / Zhang, Jiao / Shao, Yanhong / Shang, Guangdong

Biotechnology letters

2023 Volume 45, Issue 11-12, Page(s) 1521–1528

Abstract: N‑Acetyl‑D‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of Neu5Ac involves N-acetyl-D-glucosamine 2-epimerase (AGE)-catalyzed epimerization of N-acetyl-D-glucosamine ( ... ...

Abstract	N‑Acetyl‑D‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of Neu5Ac involves N-acetyl-D-glucosamine 2-epimerase (AGE)-catalyzed epimerization of N-acetyl-D-glucosamine (GlcNAc) to N-acetyl-D-mannosamine (ManNAc), and aldolase-catalyzed condensation between ManNAc and pyruvate. Host optimization plays an important role in the whole-cell biotransformation of value-added compounds. In this study, via single-plasmid biotransformation system, we showed that the AGE gene BT0453, cloned from human gut microorganism Bacteroides thetaiotaomicron VPI-5482, showed the highest biotransformation yield among the AGE genes tested; and there is no clear Neu5Ac yield difference between the BT0453 coupled with one aldolase coding nanA gene and two nanA genes. Next, Escherichia coli chromosomal genes involved in substrate degradation, product exportation and pH change were deleted via recombineering and CRISPR/Cas9. With the final E. coli BL21(DE3) ΔnanA Δnag ΔpoxB as host, a significant 16.5% yield improvement was obtained. Furthermore, precursor (pyruvate) feeding resulted in 3.2% yield improvement, reaching 66.8% molar biotransformation. The result highlights the importance of host optimization, and set the stage for further metabolic engineering of whole-cell biotransformation of Neu5Ac.
MeSH term(s)	Humans ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Aldehyde-Lyases/metabolism ; Fructose-Bisphosphate Aldolase/metabolism ; Pyruvic Acid/metabolism ; Biotransformation ; N-Acetylneuraminic Acid/metabolism
Chemical Substances	Aldehyde-Lyases (EC 4.1.2.-) ; Fructose-Bisphosphate Aldolase (EC 4.1.2.13) ; Pyruvic Acid (8558G7RUTR) ; N-Acetylneuraminic Acid (GZP2782OP0)
Language	English
Publishing date	2023-09-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	423853-9
ISSN	1573-6776 ; 0141-5492
ISSN (online)	1573-6776
ISSN	0141-5492
DOI	10.1007/s10529-023-03426-3
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Article ; Online: Structure and allergenicity of α-lactalbumin: effects of ultrasonic prior to glycation and subsequent phosphorylation

Chen, Wenmei / Chen, Qiongzhen / Zhou, Houze / Shao, Yanhong / Wang, Yang / Liu, Jun / Tu, Zongcai

Food Science and Human Wellness. 2023 May, v. 12, no. 3 p.825-831

2023

Abstract: Bovine α-lactalbumin (BLA) induced severe cow's milk allergy. In this study, a novel strategy combining ultrasonication, performed before glycation, and phosphorylation was proposed to reduce BLA allergenicity. Result showed that IgE- and IgG-binding ... ...

Abstract	Bovine α-lactalbumin (BLA) induced severe cow's milk allergy. In this study, a novel strategy combining ultrasonication, performed before glycation, and phosphorylation was proposed to reduce BLA allergenicity. Result showed that IgE- and IgG-binding capacities and the release rates of histamine and interleukin-6 from RBL-2H3 were reduced. Moreover, intrinsic fluorescence intensity and surface hydrophobicity were decreased, whereas glycated sites (R10, N44, K79, K108, N102 and K114) and phosphorylated sites (Y36 and S112) of BLA were increased. Minimum allergenicity was detected during BLA treatment after ultrasonic prior to glycation and subsequent phosphorylation because of considerable increase in glycated and phosphorylated sites. Therefore, the decrease in allergenicity of BLA, the effect correlated well with the shielding effect of glycated sites combined with phosphorylated sites and the conformational changes. This study provides important theoretical foundations for improving and using the ultrasonic technology combined with protein modification in allergenic protein processing.
Keywords	allergenicity ; allergens ; cattle ; fluorescence ; food science ; glycation ; histamine ; humans ; hydrophobicity ; interleukin-6 ; milk ; milk allergy ; phosphorylation ; ultrasonic treatment ; ultrasonics ; Bovine α-lactalbumin ; Ultrasonic ; Protein modification
Language	English
Dates of publication	2023-05
Size	p. 825-831.
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2712869-6
ISSN	2213-4530
ISSN	2213-4530
DOI	10.1016/j.fshw.2022.09.021
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: l-lysine modified whey protein isolate: Mechanism on the improvement of the gel properties and its application as model fish sausage

Su, Ling-Xia / Chen, Jing / Liu, Jun / Shao, Yan-Hong / Tu, Zong-Cai

Food Hydrocolloids. 2023 Dec., v. 145 p.109114-

2023

Abstract: In this study, whey protein isolate (WPI) was used as raw material to study the effects of l-lysine (Lys) on the gel properties and structure of WPI, and the application of modified WPI in fish sausage was studied. The results showed that with the ... ...

Abstract	In this study, whey protein isolate (WPI) was used as raw material to study the effects of l-lysine (Lys) on the gel properties and structure of WPI, and the application of modified WPI in fish sausage was studied. The results showed that with the increase of Lys concentration, the gel properties and water holding capacity of WPI were greatly improved, while the Lys concentration exceeding 4 mg/mL would produce adverse reactions, such as the decrease of chromaticity and gel hardness. At Lys concentration of 1.6 mg/mL, WPI showed the highest texture properties and good water holding capacity, which depended on the dense microstructure and reduced pore size. This was because WPI crosslinks with Lys through electrostatic interaction, reducing electrostatic repulsion and promoting its crosslinking and polymerization, which was manifested by the change of WPI structure and the increase of particle size. In addition, the hardness, chewiness, and cooking loss capacity of the surimi added with modified WPI were better than those of the pure fish sausage, corn starch and WPI single treatment group. This study can provide a theoretical basis for the study of WPI modified gel and provide guidance for the modified WPI used as gelling agent in surimi products.
Keywords	chewiness ; color ; corn starch ; crosslinking ; electrostatic interactions ; fish ; gels ; hardness ; hydrocolloids ; lysine ; microstructure ; particle size ; polymerization ; porosity ; raw materials ; sausages ; surimi ; texture ; whey protein isolate ; WPI ; l-lysine ; Gel properties ; Surimi sausage ; Application
Language	English
Dates of publication	2023-12
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	742742-6
ISSN	1873-7137 ; 0268-005X
ISSN (online)	1873-7137
ISSN	0268-005X
DOI	10.1016/j.foodhyd.2023.109114
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Gut Microbiome-Serum Metabolism Revealed the Allergenicity of Ferulic Acid Combined with Glucose-Modified β-Lactoglobulin.

Wang, Yang / Zhang, Kai / Chen, Wen-Mei / Mao, Ji-Hua / Wang, Xu-Mei / Shao, Yan-Hong / Tu, Zong-Cai / Liu, Jun

Journal of agricultural and food chemistry

2024

Abstract: A novel strategy combining ferulic acid and glucose was proposed to reduce β-lactoglobulin (BLG) allergenicity and investigate whether the reduction in allergenicity was associated with gut microbiome and serum metabolism. As a result, the multistructure ...

Abstract	A novel strategy combining ferulic acid and glucose was proposed to reduce β-lactoglobulin (BLG) allergenicity and investigate whether the reduction in allergenicity was associated with gut microbiome and serum metabolism. As a result, the multistructure of BLG changed, and the modified BLG decreased significantly the contents of IgE, IgG, IgG
Language	English
Publishing date	2024-05-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.4c01545
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Zs.A 1172: Show issues

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Article: Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks.

Wang, Titi / Chen, Wenmei / Shao, Yanhong / Liu, Jun / Tu, Zongcai

Foods (Basel, Switzerland)

2022 Volume 11, Issue 7

Abstract: The present study aims to investigate the effects of ultrasound on the non-covalent interaction of β-lactoglobulin (β-LG) and luteolin (LUT) and to investigate the relationship between allergenicity and human intestinal microbiota. After treatment, the ... ...

Abstract	The present study aims to investigate the effects of ultrasound on the non-covalent interaction of β-lactoglobulin (β-LG) and luteolin (LUT) and to investigate the relationship between allergenicity and human intestinal microbiota. After treatment, the conformational structures of β-LG were changed, which reflected by the decrease in α-helix content, intrinsic fluorescence intensity and surface hydrophobicity, whereas the β-sheet content increased. Molecular docking studies revealed the non-covalent interaction of β-LG and LUT by hydrogen bond, van der Walls bond and hydrophobic bond. β-LG-LUT complex treated by ultrasound has a lower IgG/IgE binding ability and inhibits the allergic reaction of KU812 cells, depending on the changes in the conformational epitopes of β-LG. Meanwhile, the β-LG-LUT complex affected the composition of human intestinal microbiota, such as the relative abundance of
Language	English
Publishing date	2022-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods11070988
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Article ; Online: A comparative study on the allergenic potential of β-lactoglobulin conjugated to glucose, caffeic acid and caffeoyl glucopyranose.

Wang, Yang / Zhang, Kai / Mao, Yi-Ying / Shao, Yan-Hong / Liu, Jun / Tu, Zong-Cai

Food & function

2023 Volume 14, Issue 9, Page(s) 4354–4367

Abstract: This work intends to perform a comparative study on the allergenic potential of β-lactoglobulin (BLG)-glucose, BLG-caffeic acid and BLG-caffeoyl glucopyranose conjugates. The modifications changed the molecular weight and multi-structure of BLG and ... ...

Abstract	This work intends to perform a comparative study on the allergenic potential of β-lactoglobulin (BLG)-glucose, BLG-caffeic acid and BLG-caffeoyl glucopyranose conjugates. The modifications changed the molecular weight and multi-structure of BLG and destroyed the allergenic epitope, which resulted in a decrease in the IgE binding level and the release ability of histamine and IL-6 in KU812 cells. Compared with BLG, the conjugates reduced the serum levels of IgG, IgE, β-Hex and IL-4
MeSH term(s)	Humans ; Allergens ; Glucose ; Lactoglobulins ; Hypersensitivity ; Immunoglobulin E
Chemical Substances	Allergens ; Glucose (IY9XDZ35W2) ; Lactoglobulins ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2023-05-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2612033-1
ISSN	2042-650X ; 2042-6496
ISSN (online)	2042-650X
ISSN	2042-6496
DOI	10.1039/d3fo00195d
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