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  1. Article ; Online: Electrical Signals at the Plasma Membrane and Their Influence on Chlorophyll Fluorescence of Chara Chloroplasts in vivo.

    Bulychev, Alexander A / Shapiguzov, Stepan Yu / Alova, Anna V

    Biochemistry. Biokhimiia

    2023  Volume 88, Issue 10, Page(s) 1455–1466

    Abstract: Action potentials of plant cells are engaged in the regulation of many cell processes, including photosynthesis and cytoplasmic streaming. Excitable cells of characean algae submerged in a medium with an elevated K+ content are capable of generating ... ...

    Abstract Action potentials of plant cells are engaged in the regulation of many cell processes, including photosynthesis and cytoplasmic streaming. Excitable cells of characean algae submerged in a medium with an elevated K+ content are capable of generating hyperpolarizing electrical responses. These active responses of plasma membrane originate upon the passage of inward electric current comparable in strength to natural currents circulating in illuminated Chara internodes. So far, it remained unknown whether the hyperpolarizing electrical signals in Chara affect the photosynthetic activity. Here, we showed that the negative shift of cell membrane potential, which drives K+ influx into the cytoplasm, is accompanied by a delayed decrease in the actual yield of chlorophyll fluorescence F' and the maximal fluorescence yield F
    MeSH term(s) Chara/metabolism ; Fluorescence ; Hydrogen-Ion Concentration ; Chloroplasts/metabolism ; Photosynthesis ; Cell Membrane/metabolism ; Chlorophyll/metabolism
    Chemical Substances Chlorophyll (1406-65-1)
    Language English
    Publishing date 2023-12-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1109-5
    ISSN 1608-3040 ; 0006-2979 ; 0320-9717
    ISSN (online) 1608-3040
    ISSN 0006-2979 ; 0320-9717
    DOI 10.1134/S0006297923100048
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article ; Online: Plasma membrane-chloroplast interactions activated by the hyperpolarizing response in characean cells.

    Bulychev, Alexander A / Krupenina, Natalia A / Shapiguzov, Stepan Yu / Alova, Anna V

    Plant physiology and biochemistry : PPB

    2023  Volume 201, Page(s) 107836

    Abstract: Signaling pathways in plant cells often comprise electrical phenomena developing at the plasma membrane. The action potentials in excitable plants like characean algae have a marked influence on photosynthetic electron transport and ... ...

    Abstract Signaling pathways in plant cells often comprise electrical phenomena developing at the plasma membrane. The action potentials in excitable plants like characean algae have a marked influence on photosynthetic electron transport and CO
    Language English
    Publishing date 2023-06-13
    Publishing country France
    Document type Journal Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2023.107836
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Plasma membrane–chloroplast interactions activated by the hyperpolarizing response in characean cells

    Bulychev, Alexander A. / Krupenina, Natalia A. / Shapiguzov, Stepan Yu / Alova, Anna V.

    Plant Physiology and Biochemistry. 2023 Aug., v. 201 p.107836-

    2023  

    Abstract: Signaling pathways in plant cells often comprise electrical phenomena developing at the plasma membrane. The action potentials in excitable plants like characean algae have a marked influence on photosynthetic electron transport and CO₂ assimilation. The ...

    Abstract Signaling pathways in plant cells often comprise electrical phenomena developing at the plasma membrane. The action potentials in excitable plants like characean algae have a marked influence on photosynthetic electron transport and CO₂ assimilation. The internodal cells of Characeae can also generate active electrical signals of a different type. The so called hyperpolarizing response develops under the passage of electric current whose strength is comparable to physiological currents circulating between nonuniform cell regions. The plasma membrane hyperpolarization is involved in multiple physiological events in aquatic and terrestrial plants. The hyperpolarizing response may represent an unexplored tool for studying the plasma membrane–chloroplast interactions in vivo. This study shows that the hyperpolarizing response of Chara australis internodes whose plasmalemma was preliminary converted into the K⁺-conductive state induces transient changes in maximal (Fₘ′) and actual (F′) fluorescence yields of chloroplasts in vivo. These fluorescence transients were light dependent, suggesting their relation to photosynthetic electron and H⁺ transport. The cell hyperpolarization promoted H⁺ influx that was inactivated after a single electric stimulus. The results indicate that the plasma membrane hyperpolarization drives transmembrane ion fluxes and modifies the ionic composition of cytoplasm, which indirectly (via envelope transporters) affects the pH of chloroplast stroma and chlorophyll fluorescence. Remarkably, the functioning of envelope ion transporters can be revealed in short-term experiments in vivo, without growing plants on solutions with various mineral compositions.
    Keywords Chara ; carbon dioxide ; chlorophyll ; chloroplasts ; electric current ; fluorescence ; pH ; photosynthetic electron transport ; plant physiology ; plasma membrane ; Chara internodal cells ; Electrical Signaling ; Hyperpolarizing response ; K+-conductive state ; Chlorophyll fluorescence transients ; Plasma membrane H+/OH− transport ; Plasma membrane–chloroplast interactions
    Language English
    Dates of publication 2023-08
    Publishing place Elsevier Masson SAS
    Document type Article ; Online
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2023.107836
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2696: Show issues
    Z 2005/727: Show issues

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  4. Article ; Online: Effects of chloroplast-cytoplasm exchange and lateral mass transfer on slow induction of chlorophyll fluorescence in Characeae.

    Bulychev, Alexander A / Cherkashin, Alexander A / Shapiguzov, Stepan Yu / Alova, Anna V

    Physiologia plantarum

    2021  Volume 173, Issue 4, Page(s) 1901–1913

    Abstract: Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause ... ...

    Abstract Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause transient changes in chlorophyll fluorescence at cell areas that are exposed to dim light or placed shortly in darkness. The largest distance to which the photometabolites can be transported has not yet been determined. Neither is it known if lateral transport has an influence on the induction of chlorophyll fluorescence. In this study, the relations between spatial connectivity of anchored chloroplasts in characean internodes and fluorescence induction curves were examined. Connectivity between remote cell parts was pronounced upon illumination of a cell spot at a distance up to 10 mm from the area of fluorescence measurement, provided the spot was located upstream in the cytoplasmic flow. Spatial interactions between distant cell sites were also manifested in strikingly different slow stages of fluorescence induction caused by narrow- and wide-field illumination. Cytochalasin D, cooling of bath solution, and inactivation of light-dependent envelope transporters were used to disturb cyclosis-mediated spatial interactions. Although fluorescence induction curves induced by narrow- and wide-field illumination differed greatly under control conditions, they became similar after the inhibition of cyclosis with cytochalasin D. The results indicate that cytoplasmic streaming not only drives the lateral translocation of photometabolites but also promotes the export of reducing power from illuminated chloroplasts due to flushing the chloroplast surface and keeping sharp concentration gradients.
    MeSH term(s) Chara ; Characeae ; Chlorophyll ; Chloroplasts ; Cytoplasm ; Fluorescence ; Hydrogen-Ion Concentration
    Chemical Substances Chlorophyll (1406-65-1)
    Language English
    Publishing date 2021-08-26
    Publishing country Denmark
    Document type Journal Article
    ZDB-ID 2020837-6
    ISSN 1399-3054 ; 0031-9317
    ISSN (online) 1399-3054
    ISSN 0031-9317
    DOI 10.1111/ppl.13531
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Effects of chloroplast–cytoplasm exchange and lateral mass transfer on slow induction of chlorophyll fluorescence in Characeae

    Bulychev, Alexander A. / Cherkashin, Alexander A. / Shapiguzov, Stepan Yu / Alova, Anna V.

    Physiologia plantarum. 2021 Dec., v. 173, no. 4

    2021  

    Abstract: Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause ... ...

    Abstract Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause transient changes in chlorophyll fluorescence at cell areas that are exposed to dim light or placed shortly in darkness. The largest distance to which the photometabolites can be transported has not yet been determined. Neither is it known if lateral transport has an influence on the induction of chlorophyll fluorescence. In this study, the relations between spatial connectivity of anchored chloroplasts in characean internodes and fluorescence induction curves were examined. Connectivity between remote cell parts was pronounced upon illumination of a cell spot at a distance up to 10 mm from the area of fluorescence measurement, provided the spot was located upstream in the cytoplasmic flow. Spatial interactions between distant cell sites were also manifested in strikingly different slow stages of fluorescence induction caused by narrow‐ and wide‐field illumination. Cytochalasin D, cooling of bath solution, and inactivation of light‐dependent envelope transporters were used to disturb cyclosis‐mediated spatial interactions. Although fluorescence induction curves induced by narrow‐ and wide‐field illumination differed greatly under control conditions, they became similar after the inhibition of cyclosis with cytochalasin D. The results indicate that cytoplasmic streaming not only drives the lateral translocation of photometabolites but also promotes the export of reducing power from illuminated chloroplasts due to flushing the chloroplast surface and keeping sharp concentration gradients.
    Keywords Characeae ; chlorophyll ; chloroplasts ; cytochalasin D ; cytoplasmic streaming ; exports ; fluorescence ; light intensity ; lighting ; liquids ; mass transfer ; metabolites
    Language English
    Dates of publication 2021-12
    Size p. 1901-1913.
    Publishing place Blackwell Publishing Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2020837-6
    ISSN 1399-3054 ; 0031-9317
    ISSN (online) 1399-3054
    ISSN 0031-9317
    DOI 10.1111/ppl.13531
    Database NAL-Catalogue (AGRICOLA)

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