LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 14

Search options

  1. Article: Parafilm as an efficient transport matrix for corneal scrapings.

    Sharma, Chayan / Khurana, Sumeeta

    Tropical parasitology

    2023  Volume 13, Issue 2, Page(s) 84–88

    Abstract: Introduction: Acanthamoeba spp: Aim: The aim of the study was to explore better alternatives for transporting corneal scrapings from the point-of-care eye center to the concerned laboratories.: Materials and methods: The study used small Parafilm ( ...

    Abstract Introduction: Acanthamoeba spp
    Aim: The aim of the study was to explore better alternatives for transporting corneal scrapings from the point-of-care eye center to the concerned laboratories.
    Materials and methods: The study used small Parafilm (Bemis Company Inc., USA) squares (PSs) of 1 cm each prepared by cutting Parafilm using a surgical blade under sterile conditions. Each of the four different dilutions of
    Results and conclusion: All three diagnostic techniques, i.e. microscopy, culture, and PCR, detected the presence of
    Language English
    Publishing date 2023-09-18
    Publishing country India
    Document type Journal Article
    ZDB-ID 2628177-6
    ISSN 2229-7758 ; 2229-5070
    ISSN (online) 2229-7758
    ISSN 2229-5070
    DOI 10.4103/tp.tp_67_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Acanthamoeba keratitis: Different hues of the parasite.

    Khurana, Sumeeta / Sharma, Chayan

    Indian journal of medical microbiology

    2021  Volume 40, Issue 1, Page(s) 150–151

    MeSH term(s) Acanthamoeba ; Acanthamoeba Keratitis/diagnosis ; Amebiasis ; Animals ; Humans ; Parasites
    Language English
    Publishing date 2021-11-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.1016/j.ijmmb.2021.10.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2639: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: The gene expression and proteomic profiling of Acanthamoeba isolates.

    Sharma, Chayan / Khurana, Sumeeta / Bhatia, Alka / Arora, Amit / Gupta, Amit

    Experimental parasitology

    2023  Volume 255, Page(s) 108630

    Abstract: Introduction: The free-living protozoan Acanthamoeba can cause severe keratitis known as Acanthamoeba Keratitis (AK) and granulomatous amoebic encephalitis (GAE). The pathogenesis of Acanthamoeba includes intricate interactions between the organism and ... ...

    Abstract Introduction: The free-living protozoan Acanthamoeba can cause severe keratitis known as Acanthamoeba Keratitis (AK) and granulomatous amoebic encephalitis (GAE). The pathogenesis of Acanthamoeba includes intricate interactions between the organism and the host's immune system. The downstream analysis of a well-annotated genome assembly along with proteomic analysis can unravel several biological processes and aid in the identification of potential genes involved in pathogenicity.
    Methods: Based on the next-generation sequencing data analysis, genes including lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein were selected as probable pathogenic targets that were validated by conventional PCR in a total of 30 Acanthamoeba isolates. This was followed by real-time PCR for the evaluation of relative gene expression in the keratitis and amoebic encephalitis animal model induced using keratitis (CHA5), encephalitis (CHA24) and non-pathogenic environmental isolate (CHA36). In addition, liquid chromatography-mass spectrometry (LC-MS/MS) was performed for keratitis, encephalitis, and non-pathogenic environmental isolate before and after treatment with polyhexamethylene biguanide (PHMB).
    Results: The conventional PCR demonstrated the successful amplification of lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein genes in clinical and environmental isolates. The expression analysis revealed phospholipase, lysophospholipase, and mannose-binding genes to be significantly upregulated in the keratitis isolate (CHA 5) during AK in the animal model. In the case of the amoebic encephalitis model, phospholipase, lysophospholipase, S8/S53 peptidase, and carboxylesterase were significantly upregulated in the encephalitis isolate compared to the keratitis isolate. The proteomic data revealed differential protein expression in pathogenic versus non-pathogenic isolates in the pre and post-treatment with PHMB.
    Conclusion: The gene expression data suggests that lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein (MBP) could play a role in the contact-dependent and independent mechanisms of Acanthamoeba pathogenesis. In addition, the proteomic profiling of the 3 isolates revealed differential protein expression crucial for parasite growth, survival, and virulence. Our results provide baseline data for selecting possible pathogenic targets that could be utilized for designing knockout experiments in the future.
    MeSH term(s) Animals ; Acanthamoeba ; Mannose-Binding Lectin ; Lysophospholipase/genetics ; Chromatography, Liquid ; Proteomics ; Tandem Mass Spectrometry ; Acanthamoeba Keratitis/parasitology ; Amebiasis/parasitology ; Encephalitis ; Real-Time Polymerase Chain Reaction ; Gene Expression ; Peptide Hydrolases
    Chemical Substances Mannose-Binding Lectin ; Lysophospholipase (EC 3.1.1.5) ; polihexanide (322U039GMF) ; Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2023-10-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391089-1
    ISSN 1090-2449 ; 0014-4894
    ISSN (online) 1090-2449
    ISSN 0014-4894
    DOI 10.1016/j.exppara.2023.108630
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.159: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Real-time loop-mediated isothermal amplification for the detection of Giardia duodenalis in fecal specimens.

    Khurana, Sumeeta / Sharma, Chayan / Guleria, Sucheta / Mewara, Abhishek / Dutta, Usha

    Indian journal of medical microbiology

    2023  Volume 47, Page(s) 100492

    Abstract: Introduction: Giardiasis is a leading cause of subacute or chronic diarrhoea and is frequently associated with impaired physical, cognitive and psychosocial development, especially in children. The diagnosis relies mainly on the microscopic evaluation ... ...

    Abstract Introduction: Giardiasis is a leading cause of subacute or chronic diarrhoea and is frequently associated with impaired physical, cognitive and psychosocial development, especially in children. The diagnosis relies mainly on the microscopic evaluation of stool specimens that have a low sensitivity. In contrast, molecular advancements like the polymerase chain reaction and Real-time loop-mediated isothermal amplification (Real-time LAMP) are promising techniques and reportedly have better diagnostic characteristics.
    Methods: We have evaluated the performance of Real-time LAMP for detecting Giardia in ninety stool specimens compared to microscopy and nested PCR.
    Results: A total of 35 fecal samples were detected positive by microscopy, 41 by nested PCR and 43 by real-time LAMP. Microscopy and nested PCR detected 33, microscopy and real-time LAMP detected 35, and nested PCR and real-time LAMP detected 41 positive samples.
    Conclusion: The real-time LAMP assay was found suitable for the rapid and accurate detection of G. duodenalis with a better sensitivity in comparison to nested PCR and microscopy. Furthermore, besides being sensitive and rapid, LAMP had the advantage of an adequate rapid turn-around time of eleven to 15 ​min as compared to 5 ​h of nested PCR.
    MeSH term(s) Child ; Humans ; Giardia lamblia/genetics ; Sensitivity and Specificity ; Nucleic Acid Amplification Techniques/methods ; Polymerase Chain Reaction ; Molecular Diagnostic Techniques/methods
    Language English
    Publishing date 2023-11-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.1016/j.ijmmb.2023.100492
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2639: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Environmental water surveillance for free-living amoeba in North India.

    Krishnamoorthi, Sivanantham / Sharma, Chayan / Mewara, Abhishek / Khurana, Sumeeta

    Indian journal of medical microbiology

    2022  Volume 40, Issue 3, Page(s) 389–393

    Abstract: Purpose: Free-living amoebae (FLA) exist in various environmental sources and are the causative agents of many serious human diseases. We surveyed various natural and artificial water bodies in areas of north India to detect the presence of FLA species.! ...

    Abstract Purpose: Free-living amoebae (FLA) exist in various environmental sources and are the causative agents of many serious human diseases. We surveyed various natural and artificial water bodies in areas of north India to detect the presence of FLA species.
    Materials and methods: Surface water samples were collected from 29 water bodies and subjected to microscopy, non-nutrient agar (NNA) culture, and various polymerase chain reaction (PCR) assays for the detection of FLA.
    Results: FLA were found in 62.1% (18/29) of the natural and artificial water bodies by NNA culture. Based on PCR based detection, Acanthamoeba spp. was identified in 41.4% (12/29), Vannella spp./Vahlkampfia ovis in 17.2% (5/29) and Naegleria spp. in 20.7% (6/29) of the samples from different locations. PCR-sequencing of Naegleria positive samples identified them as Naegleria australiensis and Naegleria species.
    Conclusions: The present study reports widespread occurrence of medically important FLA species in various natural and artificial water bodies, and highlights the need for regular molecular surveillance of FLA in the environment for better control and prevention of the diseases caused by FLA.
    MeSH term(s) Acanthamoeba/genetics ; Amoeba ; Animals ; Humans ; Naegleria ; Polymerase Chain Reaction ; Sheep ; Water
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2022-05-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.1016/j.ijmmb.2022.05.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2639: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Assessment of pathogenic potential of Acanthamoeba isolates by in vitro and in vivo tests.

    Sharma, Chayan / Khurana, Sumeeta / Megha, Kirti / Thakur, Anchal / Bhatia, Alka / Gupta, Amit

    Parasitology research

    2023  Volume 122, Issue 9, Page(s) 2109–2118

    Abstract: Acanthamoeba are free-living protozoa present ubiquitously in numerous environmental reservoirs that exist as an actively feeding trophozoite or a dormant cyst stage. The pathogenic Acanthamoeba are known to cause Acanthamoeba keratitis (AK) and ... ...

    Abstract Acanthamoeba are free-living protozoa present ubiquitously in numerous environmental reservoirs that exist as an actively feeding trophozoite or a dormant cyst stage. The pathogenic Acanthamoeba are known to cause Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). Despite their omnipresence, the number of infections is quite low. The reason behind this low frequency of Acanthamoeba infections could be the existence of many non-pathogenic strains or a successful host immune response to these infections. Studies in the past have proposed a few physiological parameters for the differentiation of pathogenic and non-pathogenic strains. Additionally, in vivo experiments are known to play an essential role in understanding the virulence of parasites, immunological aspects, and disease pathogenesis. The thermotolerance (30 °C, 37 °C, and 40 °C) and osmotolerance (0.5 M, 1 M, and 1.5 M) tests were performed on 43 Acanthamoeba isolates from patients with keratitis (n = 22), encephalitis (n = 5), and water samples (n = 16). In addition, the genotype of 10 Acanthamoeba isolates (keratitis (n = 2), encephalitis (n = 2), water (n = 6)) was determined and were then evaluated for pathogenicity on mouse model by inducing Acanthamoeba keratitis and amoebic encephalitis. The results of the thermotolerance and osmotolerance assays categorized 29/43 (67.4%) isolates as pathogenic, 8 as low pathogenic (18.6%), and the remaining 6 (13.9%) as non-pathogenic. The 10 Acanthamoeba isolates were categorized as T11 (5 isolates), T5 (2 isolates), T4 (2 isolates), and T10 (1 isolate) genotypes. Out of 10 Acanthamoeba isolates, 9 were successful in establishing AK, amoebic encephalitis, or both in the mice model, and a single isolate was found non-pathogenic. Two isolates from water samples were non-pathogenic in the physiological tests but successfully established Acanthamoeba infection in the mice model. The results of the physiological assays and in vivo experiments were analogous for 7 isolates while 1 isolate from the water was low pathogenic in the physiological assays but failed to produce pathogenicity during in vivo experiments. The physiological parameters are not very dependable to test the pathogenic potential of Acanthamoeba isolates, and thus results must always be validated by in vivo experiments. There is no infallible approach for determining the potential pathogenicity of environmental isolates of Acanthamoeba because several parameters regulate the pathogenic potential.
    MeSH term(s) Animals ; Mice ; Acanthamoeba/genetics ; Acanthamoeba Keratitis/parasitology ; Amebiasis/parasitology ; Genotype ; Encephalitis/parasitology ; Water ; Infectious Encephalitis
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2023-07-07
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-023-07910-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.124: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: An Insight into the Genome of Pathogenic and Non-Pathogenic

    Sharma, Chayan / Khurana, Sumeeta / Arora, Amit / Bhatia, Alka / Gupta, Amit

    Pathogens (Basel, Switzerland)

    2022  Volume 11, Issue 12

    Abstract: Background: Acanthamoeba: Methods: Illumina and Nanopore sequencing were performed for keratitis, encephalitis, and non-pathogenic environmental isolates. Hybrid assembly was prepared for the AK and GAE isolates, while only the Illumina reads were ... ...

    Abstract Background: Acanthamoeba
    Methods: Illumina and Nanopore sequencing were performed for keratitis, encephalitis, and non-pathogenic environmental isolates. Hybrid assembly was prepared for the AK and GAE isolates, while only the Illumina reads were utilized for a non-pathogenic environmental isolate. Protein coding genes were identified using the GeneMark-ES program and BLASTx module of Diamond used for gene prediction. Additionally, the Kyoto Encyclopedia of Genes and Genomes annotation and cluster of orthologous group's annotation using RPS-blast against the CDD database was performed. The subsequent data analysis and validation will help identify probable pathogenic genes.
    Results: The genome assemblies of 9.67, 8.34, and 8.89 GBs were reported for GAE, AK, and non-pathogenic isolate, respectively. KEGG reported 22,946 in GAE, 24,231 in keratitis, and 9367 genes in the environmental isolate. The COG annotation revealed 3232 in GAE, 3403 in keratitis, and 1314 genes in the non-pathogenic isolate.
    Conclusion: The present study has attempted to generate de novo hybrid genome assemblies of
    Language English
    Publishing date 2022-12-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11121558
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Acanthamoeba keratitis in a mouse model using a novel approach.

    Sharma, Chayan / Thakur, Anchal / Bhatia, Alka / Gupta, Amit / Khurana, Sumeeta

    Indian journal of medical microbiology

    2021  Volume 39, Issue 4, Page(s) 523–527

    Abstract: Context: Acanthamoeba is increasingly implicated in causing keratitis in patients wearing contact lens or ocular trauma and has a poor prognosis. Establishment of an animal model is critical to study the disease pathology, pathogenesis and to evaluate ... ...

    Abstract Context: Acanthamoeba is increasingly implicated in causing keratitis in patients wearing contact lens or ocular trauma and has a poor prognosis. Establishment of an animal model is critical to study the disease pathology, pathogenesis and to evaluate anti-amoebic drugs. Some studies have used contact lenses to establish Acanthamoeba keratitis (AK) in a mouse model, which is expensive and not very successful as lenses get dislodged.
    Objective: To assess the feasibility of using parafilm (Bemis Company Inc., USA) as an alternative to contact lens for the establishment of AK in the mouse model.
    Methods: Thirty-six Balb/c mice in three groups of six mice each for two strains of Acanthamoeba were used to induce AK. Three experimental approaches used were; i) Acanthamoeba impregnated contact lens, ii) Acanthamoeba impregnated parafilm and iii) scratching followed by inoculation of Acanthamoeba suspension. In all three models, tarsorrhaphy was performed. Infection was evaluated by clinical examination and also through microscopic examination of corneal scrapings and corneal sections.
    Results: AK model was successfully established with parafilm whereas only one mouse developed AK with the use of contact lens and none with scratching and Acanthamoeba inoculation.
    Conclusion: The use of parafilm is convenient, reliable and cheaper and can be considered an alternative to contact lenses to induce AK in a mouse model.
    MeSH term(s) Acanthamoeba ; Acanthamoeba Keratitis ; Animals ; Contact Lenses/adverse effects ; Disease Models, Animal ; Humans ; Mice ; Mice, Inbred BALB C ; Paraffin
    Chemical Substances Paraffin (8002-74-2)
    Language English
    Publishing date 2021-01-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.1016/j.ijmmb.2021.01.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2639: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis.

    Mewara, Abhishek / Khunger, Sandhya / Sharma, Chayan / Krishnamoorthi, Sivanantham / Singh, Shreya / Yadav, Rakesh / Khurana, Sumeeta / Sehgal, Rakesh

    Letters in applied microbiology

    2023  Volume 76, Issue 10

    Abstract: We developed a rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for two common intestinal parasites-Entamoeba histolytica and Giardia duodenalis, where early detection may be helpful. The mLAMP assay was optimized for the detection of ...

    Abstract We developed a rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for two common intestinal parasites-Entamoeba histolytica and Giardia duodenalis, where early detection may be helpful. The mLAMP assay was optimized for the detection of DNA of E. histolytica (18S rRNA gene) and G. duodenalis (Elongation factor 1 alpha gene) from standard strains by using six specific primers FIP (forward inner primer), BIP (backward inner primer), F3 (forward outer primer), B3 (backward outer primer), loopF (forward loop primer), and loopB (backward loop primer) for each gene target. The amplification time was 16-26 min for E. histolytica and 10-15 min for G. duodenalis, and the parasites could be distinguished based on melting-curve analysis for specific annealing temperatures (Tm) of 84°C-86°C and 88°C-90°C for E. histolytica and G. duodenalis, respectively. The analytical sensitivity was one fg, and no cross-reactivity with other intestinal pathogens was observed. Thus, the mLAMP assay could detect and clearly distinguish E. histolytica and G. duodenalis with a rapid turnaround time and excellent analytical sensitivity and specificity.
    MeSH term(s) Giardia lamblia/genetics ; Entamoeba histolytica/genetics ; Feces/parasitology ; Nucleic Acid Amplification Techniques ; Sensitivity and Specificity
    Language English
    Publishing date 2023-09-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1093/lambio/ovad114
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2080: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Opportunistic microsporidiosis unveiled by fine-needle aspiration cytology of cervical lymph node with literature review.

    Singh, Brijdeep / Kundu, Reetu / Sharma, Chayan / Khurana, Sumeeta / Bhujade, Harish / Singla, Neeraj / Rudramurthy, Shivaprakash M

    Diagnostic cytopathology

    2023  Volume 52, Issue 3, Page(s) E63–E68

    Abstract: Microsporidia are highly specialized obligate intracellular organisms closely related to fungi, traditionally linked to diarrheal diseases in acquired immunodeficiency syndrome patients. Over the past two decades, an increasing incidence of ... ...

    Abstract Microsporidia are highly specialized obligate intracellular organisms closely related to fungi, traditionally linked to diarrheal diseases in acquired immunodeficiency syndrome patients. Over the past two decades, an increasing incidence of extraintestinal infections affecting various organ systems, especially in immunocompromised individuals, has been observed. The report presents a unique case of lymph node microsporidiosis in a 38-year-old male, positive for human immunodeficiency virus, with coinfections of hepatitis B and C. Fine-needle aspiration cytology (FNAC) from cervical lymph node yielded pus-like, necrotic material with periodic acid-Schiff stained smear uncovering small round to oval spores on microscopy suspicious for microsporidia. Based on polymerase chain reaction and sequencing done with aspiration material, the causative agent was identified as Vittaforma corneae. This rare encounter highlights the significance of recognizing unique morphological characteristics of infectious organisms and employing appropriate ancillary techniques for precise identification. The case underscores the crucial role of FNAC in diagnosing opportunistic infections involving the lymph nodes and the growing significance of molecular tests for specific pathogen confirmation.
    MeSH term(s) Male ; Humans ; Adult ; Biopsy, Fine-Needle/methods ; Lymph Nodes/pathology ; Microsporidiosis/diagnosis ; Microsporidiosis/epidemiology ; Microsporidiosis/pathology ; Neck
    Language English
    Publishing date 2023-12-07
    Publishing country United States
    Document type Review ; Case Reports ; Journal Article
    ZDB-ID 632710-2
    ISSN 1097-0339 ; 8755-1039
    ISSN (online) 1097-0339
    ISSN 8755-1039
    DOI 10.1002/dc.25262
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2117: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top