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  1. Article ; Online: Development of Gamified, Interactive, Low-Cost, Flexible Virtual Microbiology Labs that Promote Higher-Order Thinking during Pandemic Instruction

    Wendy Dustman / Rolando Marquez / Sharon King-Keller

    Journal of Microbiology & Biology Education, Vol 22, Iss

    2021  Volume 1

    Abstract: The COVID-19 pandemic radically and without warning changed the laboratory learning environment for students and instructors. Students were faced with having to be receptive to new learning methods; instructors scrambled to devise innovative ways of ... ...

    Abstract The COVID-19 pandemic radically and without warning changed the laboratory learning environment for students and instructors. Students were faced with having to be receptive to new learning methods; instructors scrambled to devise innovative ways of providing a realistic lab experience for students. The demand for creative online teaching strategies and the expansion of gamified training platforms created an opportunity for the development of new and interactive lab experiences. Current online labs offer some elements of a “real” lab experience, but a system that incorporates all the tools needed to create a realistic, immersive lab environment has yet to be developed. This study examines using different gamification elements implemented in a PowerPoint-based platform. There was no cost associated with the virtual lab and it could be easily downloaded, increasing accessibility. In true gaming style, a student could “play” without restriction, without the limitations that accompany wet labs. Students were challenged with various scenarios throughout the lab, making choices and receiving feedback through the process. These features positively impacted student outcomes and improved engagement, as expressed in end-of-course evaluations. The implementation also stressed the need for further development of embedded assessments, competitive and interactive opportunities for students, and access to detailed learning analytics for instructors.
    Keywords Special aspects of education ; LC8-6691 ; Biology (General) ; QH301-705.5
    Subject code 028
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher American Society for Microbiology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: An X-Domain Phosphoinositide Phospholipase C (PI-PLC-like) of Trypanosoma brucei Has a Surface Localization and Is Essential for Proliferation

    Núria W. Negrão / Logan P. Crowe / Brian S. Mantilla / Rodrigo P. Baptista / Sharon King-Keller / Guozhong Huang / Roberto Docampo

    Pathogens, Vol 12, Iss 386, p

    2023  Volume 386

    Abstract: Trypanosoma brucei is the causative agent of African trypanosomiasis, a deadly disease that affects humans and cattle. There are very few drugs to treat it, and there is evidence of mounting resistance, raising the need for new drug development. Here, we ...

    Abstract Trypanosoma brucei is the causative agent of African trypanosomiasis, a deadly disease that affects humans and cattle. There are very few drugs to treat it, and there is evidence of mounting resistance, raising the need for new drug development. Here, we report the presence of a phosphoinositide phospholipase C (TbPI-PLC-like), containing an X and a PDZ domain, that is similar to the previously characterized TbPI-PLC1. TbPI-PLC-like only possesses the X catalytic domain and does not have the EF-hand, Y, and C2 domains, having instead a PDZ domain. Recombinant TbPI-PLC-like does not hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP 2 ) and does not modulate TbPI-PLC1 activity in vitro. TbPI-PLC-like shows a plasma membrane and intracellular localization in permeabilized cells and a surface localization in non-permeabilized cells. Surprisingly, knockdown of TbPI-PLC-like expression by RNAi significantly affected proliferation of both procyclic and bloodstream trypomastigotes. This is in contrast with the lack of effect of downregulation of expression of TbPI-PLC1 .
    Keywords Phospholipase C ; plasma membrane ; RNA interference ; Trypanosoma brucei ; Medicine ; R
    Subject code 572
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Serosurvey for Brucella spp. and Coxiella burnetii in animals on Caribbean islands

    Jason W. Johnson / Helene Lucas / Sharon King / Tyler Caron / Chengming Wang / Patrick J. Kelly

    Veterinary Medicine and Science, Vol 6, Iss 1, Pp 39-

    2020  Volume 43

    Abstract: Abstract To determine the prevalence of antibodies to Brucella melitensis, Brucella abortus and Coxiella burnetii in animals on Caribbean islands we obtained sera from convenience samples of cattle (C), sheep (S), goats (G) and cats (F) from Dominica (C, ...

    Abstract Abstract To determine the prevalence of antibodies to Brucella melitensis, Brucella abortus and Coxiella burnetii in animals on Caribbean islands we obtained sera from convenience samples of cattle (C), sheep (S), goats (G) and cats (F) from Dominica (C, S, G), Grenada (C, S, G), Montserrat (C, S, G), Puerto Rico (C), Nevis (C, S, G), St Kitts (C, S, G, F) and St Lucia (C, G). The sera were tested for antibodies against the Brucella spp. using commercial ELISA kits. Some sera were also tested at 1/80 for antibodies to C. burnetii using an indirect fluorescent antibody test. Positive sera were also tested at 1/640. None of 599 cattle, 462 sheep or 434 goats were positive in the Brucella ELISAs. None of 230 cattle had antibodies against C. burnetii, but one of 299 sheep was positive at 1/80 (Dominica – 1/54, 2%, 95% CI (0%–5.6%)), as were two of 314 goats, at 1/80 (Grenada – 1/53, 2%, 95% CI (0%–7.5%)) and 1/640 (St Kitts − 1/18, 5.6%, 95% CI (0%–16.7%)), and one of 34 cats, at 1/80 (St Kitts − 1/34; 3%, 95% CI (0%–8.8%)). Our data suggests that there is a very low prevalence or absence of B. melitensis and B. abortus on Caribbean islands. Coxiella burnetii, however, is present but it appears to be present on only some islands and then only at low levels. Overall, there appears to be a low threat to human and animal health from these organisms in the Caribbean.
    Keywords Brucella ; Caribbean ; Coxiella ; serosurvey ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Health care quality information liability & privilege.

    Donohue, Sharon King

    Annals of health law

    2002  Volume 11, Page(s) 147–58, table of contents

    Abstract: As the General Counsel for the National Committee for Quality Assurance, which accredits health plans and measures outcome performance, Ms. Donohue is in a unique position to comment on the use of such data in litigation against health plans and ... ...

    Abstract As the General Counsel for the National Committee for Quality Assurance, which accredits health plans and measures outcome performance, Ms. Donohue is in a unique position to comment on the use of such data in litigation against health plans and providers. After reviewing the growing tide of class-action lawsuits in this area, she argues that such information should be protected under the common law privilege of self-critical analysis, extended to third parties, because such privilege would protect and encourage quality assessment. Ms. Donohue further argues that the sharing and reporting of this information on a system-wide level will allow plans and providers to make visible headway in improving the care provided to patients, and that this benefit to all consumers clearly justifies the creation of a federal privilege to combat the abundance of state-level lawsuits against plans and providers that attempt to base liability on third-party accreditation and outcome performance data.
    MeSH term(s) Access to Information/legislation & jurisprudence ; Accreditation/legislation & jurisprudence ; Accreditation/organization & administration ; Disclosure/legislation & jurisprudence ; Humans ; Information Dissemination/legislation & jurisprudence ; Liability, Legal ; Malpractice/legislation & jurisprudence ; Managed Care Programs/legislation & jurisprudence ; Managed Care Programs/standards ; Peer Review, Health Care/legislation & jurisprudence ; Quality Assurance, Health Care/legislation & jurisprudence ; Quality Assurance, Health Care/organization & administration ; Quality Indicators, Health Care ; Safety Management ; United States
    Language English
    Publishing date 2002-10-24
    Publishing country United States
    Document type Journal Article ; Legal Case
    ZDB-ID 1169895-0
    ISSN 1075-2994
    ISSN 1075-2994
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Improving access to health care: a consensus ethical framework to guide proposals for reform.

    Levine, Mark A / Wynia, Matthew K / Schyve, Paul M / Teagarden, J Russell / Fleming, David A / Donohue, Sharon King / Anderson, Ron J / Sabin, James / Emanuel, Ezekiel J

    The Hastings Center report

    2007  Volume 37, Issue 5, Page(s) 14–19

    MeSH term(s) Consensus ; Delivery of Health Care/legislation & jurisprudence ; Delivery of Health Care/organization & administration ; Health Care Reform/ethics ; Health Services Accessibility/ethics ; Humans ; United States
    Language English
    Publishing date 2007-10-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 194940-8
    ISSN 1552-146X ; 0093-0334
    ISSN (online) 1552-146X
    ISSN 0093-0334
    DOI 10.1353/hcr.2007.0076
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens

    Esther Willems / Jolein Gloerich / Anouk Suppers / Michiel van der Flier / Lambert P. van den Heuvel / Nicole van de Kar / Ria H.L.A. Philipsen / Maurice van Dael / Myrsini Kaforou / Victoria J. Wright / Jethro A. Herberg / Federico Martinon Torres / Michael Levin / Ronald de Groot / Alain J. van Gool / Dirk J. Lefeber / Hans J.C.T. Wessels / Marien I. de Jonge / Amina Abdulla /
    Christoph Aebi / Koen van Aerde / Rachel Agbeko / Philipp Agyeman / Umberto D’alessandro / Ladan Ali / Wynand Alkema / Karen Allen / Fernando Álvez González / Suzanne Anderson / Imran Ansari / Tasnim Araf / Tanja Avramoska / Bryan Baas / Natalija Bahovec / Cristina Balo Farto / Anda Balode / A.M. Barendregt / Ruth Barral-Arca / María Barreiro Castro / Arta Bārzdiņa / David Bath / Sebastian Bauchinger / Lucas Baumard / Hinrich Baumgart / Frances Baxter / Ashley Bell / Kathryn Bell / Xabier Bello / Evangelos Bellos / Martin Benesch / Mirian Ben García / Joshua Bennet / Christoph Berger / J.M. van den Berg / Sara Bernhard-Stirnemann / Sagida Bibi / Christoph Bidlingmaier / Alexander Binder / Vera Binder / Kalifa Bojang / Dorine M. Borensztajn / Ulrich von Both / Karen Brengel-Pesce / Bryan van den Broek / Judith Buschbeck / Leo Calvo-Bado / Sandra Carnota / Enitan D. Carrol / Michael J. Carter / Miriam Cebey-López / Samba Ceesay / Astrid Ceolotto / Adora Chan / Elizabeth Cocklin / Kalvin Collings / Stephen Crulley / Aubrey Cunnington / María José Curras-Tuala / Katharina Danhauser / Saffiatou Darboe / Sarah Darnell / Tisham De / Dārta Deksne / Kirsty Devine / Juan Emmanuel Dewez / Julia Dudley / Carlos Durán Suárez / Ernst Eber / Irini Eleftheriou / Marieke Emonts / Daniel Fabian / Tobias Feuchtinger / Katy Fidler / Colin Fink / A.M. van Furth / Rachel Galassini / Siegfried Gallistl / Luisa García Vicente / Dace Gardovska / J. Geissler / G.P.J.M. Gerrits / Eric Giannoni / Ilona van der Giessen / Alberto Gómez-Carballa / Jose Gómez Rial / Gunther Gores / Dagne Grāvele / Matthias Griese / Ilze Grope / Meeru Gurung / L. de Haan / Nikolaus Haas / Dominic Habgood-Coote / Nienke N. Hagedoorn / Harald Haidl / Shea Hamilton / Almuthe Hauer / J. Heidema / Ulrich Heininger / Stefanie Henriet / Jethro Herberg / Clive Hoggart / Susanne Hösele / Sara Hourmat / Christa Hude / Martijn Huijnen / Heather Jackson / Rebecca Jennings / Joanne Johnston / Ilse Jongerius / Rikke Jorgensen / Christian Kahlert / Rama Kandasamy / Matthias Kappler / Julia Keil / Markus Keldorfer / Dominic F. Kell / Eunjung Kim / Sharon King / Lieke Kloosterhuis / Daniela S. Kohlfürst / Benno Kohlmaier / Laura Kolberg / Mojca Kolnik / Larissa Krenn / Taco Kuijpers / M. van der Kuip / Pilar Leboráns Iglesias / Simon Leigh / Manuel Leitner / M. van Leur / Emma Lim / Naomi Lin / Ching-Chuan Liu / Sabine Löffler / Eberhard Lurz / Ian Maconochie / Christine Mackerness / François Mallet / Federico Martinón-Torres / Antonis Marmarinos / Alex Martin / Mike Martin / José María Martinón Sánchez / Nazareth Martinón-Torres / Paul McAlinden / Anne McDonnell / Sam McDonald / C.J. Miedema / Anija Meiere / Stephanie Menikou / G. van Mierlo / Alec Miners / Ravi Mistry / Henriëtte A. Moll / Marine Mommert / Belén Mosquera Pérez / David R. Murdoch / Sobia Mustafa / Giancarlo Natalucci / C. Neeleman / Karen Newall / Samuel Nichols / Tobias Niedrist / Anita Niederer-Loher / Ruud Nijman / Ieva Nokalna / Urzula Nora Urbāne / Gudrun Nordberg / C.C. Obihara / Daniel O'Connor / Wilma Oosthoek / Veronika Osterman / Alexandre Pachot / D. Pajkrt / Jacobo Pardo-Seco / Stéphane Paulus / Jana Pavāre / Ivonne Pena Paz / Salina Persand / Andreas Pfleger / Klaus Pfurtscheller / Ria Philipsen / Ailsa Pickering / Benjamin Pierce / Heidemarie Pilch / Lidia Piñeiro Rodríguez / Sara Pischedda / Tina Plankar Srovin / Marko Pokorn / Andrew J. Pollard / Lena Pölz / Klara M. Posfay-Barbe / Petra Prunk / Zanda Pučuka / Glorija Rajic / Aqeela Rashid / Lorenzo Redondo-Collazo / Christa Relly / Irene Rivero Calle / Sara Rey Vázquez / Mathew Rhodes / Vivien Richmond / Thomas Riedel / Anna RocaIsatou Sarr / Siegfried Rödl / Carmen Rodríguez-Tenreiro / Sam Romaine / Emily Rowlands / Miguel Sadiki Ora / Manfred G. Sagmeister / Momodou Saidykhan / Antonio Salas / Luregn J. Schlapbach / D. Schonenberg / Fatou Secka / Katrīna Selecka / Sonia Serén Fernández / Cristina Serén Trasorras / Priyen Shah / Ching-Fen Shen / Shrijana Shrestha / Aleksandra Sidorova / Andrea Skrabl-Baumgartner / Giselle D’Souza / Matthias Sperl / Evelien Sprenkeler / Nina A. Schweintzger / Laura Stampfer / Molly Stevens / Martin Stocker / Volker Strenger / Dace Svile / Kelly Syggelou / Maria Tambouratzi / Chantal Tan / Emma Tavliavini / Evelyn Thomson / Stephen Thorson / Holger Till / G.A. Tramper-Stranders / Andreas Trobisch / Maria Tsolia / Effua Usuf / Lucille Valentine / Clementien L. Vermont / Marisol Vilas Iglesias / Katarina Vincek / Marie Voice / Gabriella de Vries / Diane Wallia / Shih-Min Wang / Clare Wilson / Amanda Wood / Phil Woodsford / Victoria Wright / Marietta Xagorari / Shunmay Yeung / Joany Zachariasse / Dace Zavadska / Syed M.A. Zaman / Judith Zandstra / Werner Zenz / Christoph Zurl / Manuela Zwerenz

    iScience, Vol 26, Iss 8, Pp 107257- (2023)

    2023  

    Abstract: Summary: Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an ... ...

    Abstract Summary: Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic proteome-wide glycosylation in response to infection. The protein site-specific glycosylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpretation of relevant biological changes in response to infection.
    Keywords Health sciences ; Glycobiology ; Immunology ; Glycomics ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2023-08-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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