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  1. Book: Mitosis

    Sharp, David J.

    methods and protocols

    (Methods in molecular biology ; 1136)

    2014  

    Author's details ed. by David J. Sharp
    Series title Methods in molecular biology ; 1136
    Collection
    Keywords Mitosis ; Mitosis/Research/Methodology
    Subject code 571.844
    Language English
    Size X, 308 S. : Ill., graph. Darst., 27 cm
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Formerly CIP. ; Includes bibliographical references and index
    HBZ-ID HT018255112
    ISBN 978-1-4939-0328-3 ; 9781493903290 ; 1-4939-0328-4 ; 1493903292
    Database Catalogue ZB MED Medicine, Health

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  2. Article ; Online: Dementia after traumatic brain injury.

    Graham, Neil Sn / Sharp, David J

    BMJ (Clinical research ed.)

    2023  Volume 383, Page(s) 2065

    MeSH term(s) Humans ; Brain Injuries, Traumatic/etiology ; Brain Injuries, Traumatic/therapy ; Dementia/etiology ; Brain
    Language English
    Publishing date 2023-10-19
    Publishing country England
    Document type Editorial
    ZDB-ID 1362901-3
    ISSN 1756-1833 ; 0959-8154 ; 0959-8146 ; 0959-8138 ; 0959-535X ; 1759-2151
    ISSN (online) 1756-1833
    ISSN 0959-8154 ; 0959-8146 ; 0959-8138 ; 0959-535X ; 1759-2151
    DOI 10.1136/bmj.p2065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The fidgetin family: Shaking things up among the microtubule-severing enzymes.

    Smart, Karishma / Sharp, David J

    Cytoskeleton (Hoboken, N.J.)

    2023  Volume 81, Issue 2-3, Page(s) 151–166

    Abstract: The microtubule cytoskeleton is required for several crucial cellular processes, including chromosome segregation, cell polarity and orientation, and intracellular transport. These functions rely on microtubule stability and dynamics, which are regulated ...

    Abstract The microtubule cytoskeleton is required for several crucial cellular processes, including chromosome segregation, cell polarity and orientation, and intracellular transport. These functions rely on microtubule stability and dynamics, which are regulated by microtubule-binding proteins (MTBPs). One such type of regulator is the microtubule-severing enzymes (MSEs), which are ATPases Associated with Diverse Cellular Activities (AAA+ ATPases). The most recently identified family are the fidgetins, which contain three members: fidgetin, fidgetin-like 1 (FL1), and fidgetin-like 2 (FL2). Of the three known MSE families, the fidgetins have the most diverse range of functions in the cell, spanning mitosis/meiosis, development, cell migration, DNA repair, and neuronal function. Furthermore, they offer intriguing novel therapeutic targets for cancer, cardiovascular disease, and wound healing. In the two decades since their first report, there has been great progress in our understanding of the fidgetins; however, there is still much left unknown about this unusual family. This review aims to consolidate the present body of knowledge of the fidgetin family of MSEs and to inspire deeper exploration into the fidgetins and the MSEs as a whole.
    MeSH term(s) Microtubule-Associated Proteins/metabolism ; Microtubules/metabolism ; ATPases Associated with Diverse Cellular Activities/genetics ; Adenosine Triphosphatases/metabolism ; Neurons/metabolism
    Chemical Substances Microtubule-Associated Proteins ; ATPases Associated with Diverse Cellular Activities (EC 3.6.4.-) ; Adenosine Triphosphatases (EC 3.6.1.-)
    Language English
    Publishing date 2023-10-12
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2534372-5
    ISSN 1949-3592 ; 1949-3584
    ISSN (online) 1949-3592
    ISSN 1949-3584
    DOI 10.1002/cm.21799
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: In Vitro Screening Assay for Activators of T-Cell Migration to Solid Tumors.

    Dey, Abhinav / Sharp, David J

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2423, Page(s) 109–113

    Abstract: Recently, chimeric antigen receptor (CAR) T-cell therapy has begun to be used for solid tumors such as glioblastoma multiforme. Pediatric malignant brain tumors patients develop extensive long-term morbidity of intensive multimodal curative treatment. ... ...

    Abstract Recently, chimeric antigen receptor (CAR) T-cell therapy has begun to be used for solid tumors such as glioblastoma multiforme. Pediatric malignant brain tumors patients develop extensive long-term morbidity of intensive multimodal curative treatment. CAR T-cells treatments could potentially create favorable outcomes and reduce the toxicity of current treatment. T-cell infiltration of solid tumors has been associated with good prognosis. A largely overlooked area of CAR T-cell therapy targeting solid tumors is enhancing the ability of CAR T-cells to migrate and infiltrate solid tumors. A potential reason could be lack of standard in vitro assays which can screen for genetic modifications that result in enhanced T-cell migration in CAR T-cell therapies. We report a novel coculture assay using 3D tumor spheroids cocultured with T-cells to analyze the effect of activating CAR T-cell interventions on cell migration by a simple imaging based readout. This assay can be applied to several different kinds of cancer cell lines in higher throughput as well as toward measuring the efficiency of currently available CAR T-cell therapies in untested solid tumors.
    MeSH term(s) Cell Movement ; Child ; Glioblastoma/metabolism ; Glioblastoma/therapy ; Humans ; Immunotherapy, Adoptive/methods ; Receptors, Chimeric Antigen/genetics ; Receptors, Chimeric Antigen/metabolism ; T-Lymphocytes
    Chemical Substances Receptors, Chimeric Antigen
    Language English
    Publishing date 2022-01-03
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1952-0_11
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Clinical outcomes evolve years after traumatic brain injury.

    Sharp, David J / Graham, Neil S N

    Nature reviews. Neurology

    2023  Volume 19, Issue 10, Page(s) 579–580

    MeSH term(s) Humans ; Brain Injuries, Traumatic/therapy
    Language English
    Publishing date 2023-09-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 2491514-2
    ISSN 1759-4766 ; 1759-4758
    ISSN (online) 1759-4766
    ISSN 1759-4758
    DOI 10.1038/s41582-023-00868-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A PRISMA Systematic Review on the Safety and Efficacy of Patient-Controlled Analgesia (PCA) in Pediatrics.

    Sharp, David / Jaffrani, Adrianna

    Journal of pediatric nursing

    2021  Volume 61, Page(s) 219–223

    Abstract: Problem: Effective pain management is the key to improving not only patient outcomes but also patient satisfaction. Patient controlled analgesia (PCA) is a pain management method that allows the patient to self-administer their medication. Because of ... ...

    Abstract Problem: Effective pain management is the key to improving not only patient outcomes but also patient satisfaction. Patient controlled analgesia (PCA) is a pain management method that allows the patient to self-administer their medication. Because of the great variety of physical and cognitive abilities in the pediatric population, involvement of a nurse or parent proxy is necessary.
    Purpose: The purpose of this study was to ascertain the most effective approaches to PCA in pediatric settings.
    Eligibility criteria: Criteria for articles selection were as follows: (a) published in a peer-review journal, (b) between 2014 and 2019, (c) in English, (d) directly addressing the issues of safety and efficacy of patient-controlled analgesia by proxy in the pediatric patient population.
    Sample: Databases used in the search included CINAHL Plus with Full Text, DynaMed, MedLine with Full Text, and ScienceDirect. Combinations of the following keywords were used to search each database: "nurse controlled analgesia", "parent controlled analgesia", "patient controlled analgesia by proxy", "NCA", "P/NCA", "PCA by proxy", "pediatrics", "children", "pediatric patients". Database searches yielded 172 results. Articles that were duplicated, missing inclusion criteria or did not directly address the issues of safety and efficacy of PCA by proxy were removed. Eleven articles fit the selection criteria.
    Results: Eleven articles were included in the final report. The themes that emerged from the analysis included pain management of neonates and infants, children with developmental disabilities, children with cancer, as well as the sources and possible solutions to errors in medication preparation.
    Conclusions: It was concluded that PCA by proxy remains a safe and efficient method of pain administration for the pediatric population, with the exception of children suffering from developmental and neurological disabilities.
    Implications: PCA by proxy, although presenting challenges, remains a safe and efficient way of pain management across different pediatric populations, such as infants and neonates or children with cancer, both inpatient and outpatient, and new technologies could positively influence the safety of this method of pain management. Conversely, children with developmental and neurological disabilities do not benefit from this method of pain management and are more prone to experiencing adverse effects.
    MeSH term(s) Analgesia, Patient-Controlled ; Analgesics, Opioid ; Child ; Humans ; Infant ; Infant, Newborn ; Pain Management ; Pain, Postoperative ; Parents ; Patient Satisfaction ; Pediatrics
    Chemical Substances Analgesics, Opioid
    Language English
    Publishing date 2021-06-15
    Publishing country United States
    Document type Journal Article ; Systematic Review
    ZDB-ID 632731-x
    ISSN 1532-8449 ; 0882-5963
    ISSN (online) 1532-8449
    ISSN 0882-5963
    DOI 10.1016/j.pedn.2021.06.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: mRNA Localization and Local Translation of the Microtubule Severing Enzyme, Fidgetin-Like 2, in Polarization, Migration and Outgrowth.

    Birnbaum, Rayna / Biswas, Jeetayu / Singer, Robert H / Sharp, David J

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Cell motility requires strict spatiotemporal control of protein expression. During cell migration, mRNA localization and local translation in subcellular areas like the leading edge and protrusions are particularly advantageous for regulating the ... ...

    Abstract Cell motility requires strict spatiotemporal control of protein expression. During cell migration, mRNA localization and local translation in subcellular areas like the leading edge and protrusions are particularly advantageous for regulating the reorganization of the cytoskeleton. Fidgetin-Like 2 (FL2), a microtubule severing enzyme (MSE) that restricts migration and outgrowth, localizes to the leading edge of protrusions where it severs dynamic microtubules. FL2 is primarily expressed during development but in adulthood, is spatially upregulated at the leading edge minutes after injury. Here, we show mRNA localization and local translation in protrusions of polarized cells are responsible for FL2 leading edge expression after injury. The data suggests that the RNA binding protein IMP1 is involved in the translational regulation and stabilization of FL2 mRNA, in competition with the miRNA let-7. These data exemplify the role of local translation in microtubule network reorganization during migration and elucidate an unexplored MSE protein localization mechanism.
    Language English
    Publishing date 2023-04-17
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.04.17.537087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Validating the plate mapping method: Comparing drawn foods and actual foods of university students in a cafeteria.

    Sharp, David E

    Appetite

    2016  Volume 100, Page(s) 197–202

    Abstract: To examine effects of plate size on meals, I developed a method I label plate mapping. To validate plate mapping, a quasi-experimental between participants study was conducted that asked university students to accurately draw their lunch meal. ... ...

    Abstract To examine effects of plate size on meals, I developed a method I label plate mapping. To validate plate mapping, a quasi-experimental between participants study was conducted that asked university students to accurately draw their lunch meal. Participants were randomized into groups where they were asked either pre-consumption or post-consumption to draw their lunch on either a 9″ or 11″ paper plate. Coding plate drawings for total meal size revealed that students drew bigger meals on larger plates and participant meal size drawings were more accurate when the plate provided was the same size as the meal plate used. Gender moderated meal size drawings, with women generally drawing meals that were more highly correlated and similarly sized to actual meals when compared to the drawings of men. Overall, the size of plate drawings was highly correlated with the size of actual foods, which provides support for the validity of the method of plate mapping. These findings suggest that plate mapping can be applied to estimate meal size and assess sensitivity to plate size. Gaining a better understanding of the mechanisms and processes that influence food consumption habits can help increase awareness of these cues for both academics as well as for consumers of food and benefit short- and long-term health goals.
    MeSH term(s) Adolescent ; Adult ; Concept Formation ; Cues ; Female ; Food Services ; Health Knowledge, Attitudes, Practice ; Healthy Diet ; Humans ; Lunch ; Male ; Nutrition Assessment ; Ohio ; Patient Compliance ; Portion Size ; Postprandial Period ; Random Allocation ; Sex Characteristics ; Students ; Truth Disclosure ; Universities ; Young Adult
    Language English
    Publishing date 2016-05-01
    Publishing country England
    Document type Comparative Study ; Journal Article ; Validation Studies
    ISSN 1095-8304
    ISSN (online) 1095-8304
    DOI 10.1016/j.appet.2016.02.023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book: Mitosis

    Sharp, David

    methods and protocols

    (Methods in molecular biology, ; 1136 ; Springer protocols)

    2014  

    Author's details edited by David J. Sharp
    Series title Methods in molecular biology, ; 1136
    Springer protocols
    MeSH term(s) Mitosis/physiology
    Language English
    Size x, 308 pages :, illustrations ;, 27 cm.
    Document type Book
    ISBN 9781493903283 ; 1493903284 ; 9781493903290 ; 1493903292
    Database Catalogue of the US National Library of Medicine (NLM)

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  10. Book: Mitosis

    Sharp, David

    methods and protocols

    (Methods in molecular biology, ; 1136 ; Springer protocols)

    2014  

    Abstract: Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the ... ...

    Author's details edited by David J. Sharp
    Series title Methods in molecular biology, ; 1136
    Springer protocols
    Abstract Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division, and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.
    Keywords Mitosis. ; Mitosis/Research/Methodology.
    Language English
    Size x, 308 pages :, illustrations (some color) ;, 27 cm.
    Document type Book
    ISBN 9781493903283 ; 1493903284 ; 9781493903290 ; 1493903292
    Database NAL-Catalogue (AGRICOLA)

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