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Article: Niuhuang Qingxin Wan ameliorates depressive-like behaviors and improves hippocampal neurogenesis through modulating TrkB/ERK/CREB signaling pathway in chronic restraint stress or corticosterone challenge mice.

Du, Qiaohui / Gao, Chong / Tsoi, Bun / Wu, Meiling / Shen, Jiangang

2024 Volume 14, Page(s) 1274343

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2024-01-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1274343
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reactive nitrogen species as therapeutic targets for autophagy/mitophagy modulation to relieve neurodegeneration in multiple sclerosis: Potential application for drug discovery.

Li, Wenting / Wu, Meiling / Li, Yuzhen / Shen, Jiangang

Free radical biology & medicine

2023 Volume 208, Page(s) 37–51

Abstract: Multiple sclerosis (MS) is a neuroinflammatory disease with limited therapeutic effects, eventually developing into handicap. Seeking novel therapeutic strategies for MS is timely important. Active autophagy/mitophagy could mediate neurodegeneration, ... ...

Abstract	Multiple sclerosis (MS) is a neuroinflammatory disease with limited therapeutic effects, eventually developing into handicap. Seeking novel therapeutic strategies for MS is timely important. Active autophagy/mitophagy could mediate neurodegeneration, while its roles in MS remain controversial. To elucidate the exact roles of autophagy/mitophagy and reveal its in-depth regulatory mechanisms, we conduct a systematic literature study and analyze the factors that might be responsible for divergent results obtained. The dynamic change levels of autophagy/mitophagy appear to be a determining factor for final neuron fate during MS pathology. Excessive neuronal autophagy/mitophagy contributes to neurodegeneration after disease onset at the active MS phase. Reactive nitrogen species (RNS) serve as key regulators for redox-related modifications and participate in autophagy/mitophagy modulation in MS. Nitric oxide (
MeSH term(s)	Humans ; Mitophagy ; Reactive Nitrogen Species/metabolism ; Multiple Sclerosis/drug therapy ; Multiple Sclerosis/genetics ; Autophagy ; Nitric Oxide/pharmacology ; Drug Discovery
Chemical Substances	Reactive Nitrogen Species ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2023-08-01
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	807032-5
ISSN	1873-4596 ; 0891-5849
ISSN (online)	1873-4596
ISSN	0891-5849
DOI	10.1016/j.freeradbiomed.2023.07.032
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Article: Naringin Mediates Adult Hippocampal Neurogenesis for Antidepression via Activating CREB Signaling.

Gao, Chong / Wu, Meiling / Du, Qiaohui / Deng, Jiagang / Shen, Jiangang

Frontiers in cell and developmental biology

2022 Volume 10, Page(s) 731831

Abstract: The brain-derived neurotrophic factor/tropomyosin receptor kinase B/cAMP response element-binding protein (BDNF/TrkB/CREB) signaling pathway is a critical therapeutic target for inducing adult hippocampal neurogenesis and antidepressant therapy. In this ... ...

Abstract	The brain-derived neurotrophic factor/tropomyosin receptor kinase B/cAMP response element-binding protein (BDNF/TrkB/CREB) signaling pathway is a critical therapeutic target for inducing adult hippocampal neurogenesis and antidepressant therapy. In this study, we tested the hypothesis that naringin, a natural medicinal compound, could promote adult hippocampal neurogenesis and improve depression-like behaviors via regulating the BDNF/TrkB/CREB signaling pathway. We first investigated the effects of naringin on promoting adult hippocampal neurogenesis in both normal and chronic corticosterone (CORT)-induced depressive mice. Under physiological condition, naringin treatment enhanced the proliferation of neural stem/progenitor cells (NSPCs) and accelerated neuronal differentiation. In CORT-induced depression mouse model, naringin treatment promoted neuronal differentiation and maturation of NSPCs for hippocampal neurogenesis. Forced swim test, tail suspension test, and open field test confirmed the antidepressant and anxiolytic effects of naringin. Co-treatment of temozolomide (TMZ), a neurogenic inhibitor, abolished these antidepressant and anxiolytic effects. Meanwhile, naringin treatment increased phosphorylation of cAMP response element binding protein (CREB) but had no effect on the expression of brain-derived neurotrophic factor and phosphorylation of TrkB in the hippocampus of CORT-induced depressive mice. Co-treatment of CREB inhibitor 666-15, rather than TrkB inhibitor Cyc-B, abolished the neurogenesis-promoting and antidepressant effects of naringin. Taken together, naringin has antidepressant and anxiolytic effects, and the underlying mechanisms could be attributed to enhance hippocampal neurogenesis via activating CREB signaling.
Language	English
Publishing date	2022-04-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2022.731831
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Article ; Online: Targeting proteasome enhances anticancer activity of oncolytic HSV-1 in colorectal cancer.

Li, Xiaxi / Hu, Wei / Shen, Jiangang / Li, Mingsong / Gong, Wei

Virology

2022 Volume 578, Page(s) 13–21

Abstract: Herpes simplex virus 1 (HSV-1) has been widely used to treat various cancers, but its efficacy is limited. Studies indicated that combining HSV-1 and chemotherapy drugs can effectively improve the lethality of HSV-1 in tumor cells, which has a ... ...

Abstract	Herpes simplex virus 1 (HSV-1) has been widely used to treat various cancers, but its efficacy is limited. Studies indicated that combining HSV-1 and chemotherapy drugs can effectively improve the lethality of HSV-1 in tumor cells, which has a synergistic effect. Here, we explored the oncolytic effect and mechanism of bortezomib and HSV-1 on colorectal cancer cells, HCT116 and Caco-2. First, we selected four drugs to detect cell viability and found that the strongest HSV-1-promoting effect was achieved using bortezomib + HSV-1 treatment. Bortezomib combined with HSV-1 treatment significantly upregulated the expression of heat shock proteins, endoplasmic reticulum stress-related proteins and apoptosis-related proteins, while Bcl-2 was downregulated. JC-1 staining revealed that combining bortezomib and HSV-1 promotes cell apoptosis. In addition, bortezomib + oHSV-1 treatment effectively inhibit tumor growth. These results indicate that bortezomib combined with HSV-1 induce intense endoplasmic reticulum stress and activate the caspase-12 apoptosis pathway, killing tumor cells.
MeSH term(s)	Humans ; Herpesvirus 1, Human/physiology ; Bortezomib/pharmacology ; Proteasome Endopeptidase Complex ; Caco-2 Cells ; Apoptosis ; Colorectal Neoplasms/therapy ; Cell Line, Tumor ; Oncolytic Virotherapy
Chemical Substances	Bortezomib (69G8BD63PP) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2022-11-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	200425-2
ISSN	1096-0341 ; 0042-6822
ISSN (online)	1096-0341
ISSN	0042-6822
DOI	10.1016/j.virol.2022.11.002
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Article ; Online: Hypochlorous acid derived from microglial myeloperoxidase could mediate high-mobility group box 1 release from neurons to amplify brain damage in cerebral ischemia-reperfusion injury.

Chen, Shuang / Pan, Jingrui / Gong, Zhe / Wu, Meiling / Zhang, Xiaoni / Chen, Hansen / Yang, Dan / Qi, Suhua / Peng, Ying / Shen, Jiangang

Journal of neuroinflammation

2024 Volume 21, Issue 1, Page(s) 70

Abstract: Myeloperoxidase (MPO) plays critical role in the pathology of cerebral ischemia-reperfusion (I/R) injury via producing hypochlorous acid (HOCl) and inducing oxidative modification of proteins. High-mobility group box 1 (HMGB1) oxidation, particularly ... ...

Abstract	Myeloperoxidase (MPO) plays critical role in the pathology of cerebral ischemia-reperfusion (I/R) injury via producing hypochlorous acid (HOCl) and inducing oxidative modification of proteins. High-mobility group box 1 (HMGB1) oxidation, particularly disulfide HMGB1 formation, facilitates the secretion and release of HMGB1 and activates neuroinflammation, aggravating cerebral I/R injury. However, the cellular sources of MPO/HOCl in ischemic brain injury are unclear yet. Whether HOCl could promote HMGB1 secretion and release remains unknown. In the present study, we investigated the roles of microglia-derived MPO/HOCl in mediating HMGB1 translocation and secretion, and aggravating the brain damage and blood-brain barrier (BBB) disruption in cerebral I/R injury. In vitro, under the co-culture conditions with microglia BV cells but not the single culture conditions, oxygen-glucose deprivation/reoxygenation (OGD/R) significantly increased MPO/HOCl expression in PC12 cells. After the cells were exposed to OGD/R, MPO-containing exosomes derived from BV2 cells were released and transferred to PC12 cells, increasing MPO/HOCl in the PC12 cells. The HOCl promoted disulfide HMGB1 translocation and secretion and aggravated OGD/R-induced apoptosis. In vivo, SD rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) plus different periods of reperfusion. Increased MPO/HOCl production was observed at the reperfusion stage, accomplished with enlarged infarct volume, aggravated BBB disruption and neurological dysfunctions. Treatment of MPO inhibitor 4-aminobenzoic acid hydrazide (4-ABAH) and HOCl scavenger taurine reversed those changes. HOCl was colocalized with cytoplasm transferred HMGB1, which was blocked by taurine in rat I/R-injured brain. We finally performed a clinical investigation and found that plasma HOCl concentration was positively correlated with infarct volume and neurological deficit scores in ischemic stroke patients. Taken together, we conclude that ischemia/hypoxia could activate microglia to release MPO-containing exosomes that transfer MPO to adjacent cells for HOCl production; Subsequently, the production of HOCl could mediate the translocation and secretion of disulfide HMGB1 that aggravates cerebral I/R injury. Furthermore, plasma HOCl level could be a novel biomarker for indexing brain damage in ischemic stroke patients.
MeSH term(s)	Humans ; Rats ; Animals ; Hypochlorous Acid ; Microglia/metabolism ; HMGB1 Protein/metabolism ; Rats, Sprague-Dawley ; Brain Injuries/metabolism ; Brain Ischemia/metabolism ; Blood-Brain Barrier/metabolism ; Infarction, Middle Cerebral Artery/complications ; Infarction, Middle Cerebral Artery/pathology ; Neurons/metabolism ; Reperfusion Injury/metabolism ; Peroxidase/metabolism ; Ischemic Stroke ; Taurine ; Disulfides
Chemical Substances	Hypochlorous Acid (712K4CDC10) ; HMGB1 Protein ; Peroxidase (EC 1.11.1.7) ; Taurine (1EQV5MLY3D) ; Disulfides
Language	English
Publishing date	2024-03-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2156455-3
ISSN	1742-2094 ; 1742-2094
ISSN (online)	1742-2094
ISSN	1742-2094
DOI	10.1186/s12974-023-02991-8
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Article ; Online: Targeting proteasome enhances anticancer activity of oncolytic HSV-1 in colorectal cancer

Li, Xiaxi / Hu, Wei / Shen, Jiangang / Li, Mingsong / Gong, Wei

Virology. 2022 Nov. 03,

2022

Abstract	Herpes simplex virus 1 (HSV-1) has been widely used to treat various cancers, but its efficacy is limited. Studies indicated that combining HSV-1 and chemotherapy drugs can effectively improve the lethality of HSV-1 in tumor cells, which has a synergistic effect. Here, we explored the oncolytic effect and mechanism of bortezomib and HSV-1 on colorectal cancer cells, HCT116 and Caco-2. First, we selected four drugs to detect cell viability and found that the strongest HSV-1-promoting effect was achieved using bortezomib + HSV-1 treatment. Bortezomib combined with HSV-1 treatment significantly upregulated the expression of heat shock proteins, endoplasmic reticulum stress-related proteins and apoptosis-related proteins, while Bcl-2 was downregulated. JC-1 staining revealed that combining bortezomib and HSV-1 promotes cell apoptosis. In addition, bortezomib + oHSV-1 treatment effectively inhibit tumor growth. These results indicate that bortezomib combined with HSV-1 induce intense endoplasmic reticulum stress and activate the caspase-12 apoptosis pathway, killing tumor cells.
Keywords	Human alphaherpesvirus 1 ; antineoplastic activity ; apoptosis ; cell viability ; colorectal neoplasms ; death ; drug therapy ; endoplasmic reticulum ; endoplasmic reticulum stress ; heat stress ; proteasome endopeptidase complex ; synergism ; virology ; Bortezomib ; HSV-1 ; Drug combination ; Colorectal cancer cells ; Oncolysis
Language	English
Dates of publication	2022-1103
Publishing place	Elsevier Inc.
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	200425-2
ISSN	1096-0341 ; 0042-6822
ISSN (online)	1096-0341
ISSN	0042-6822
DOI	10.1016/j.virol.2022.11.002
Database	NAL-Catalogue (AGRICOLA)

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Article: In situ construction of graded porous carbon matrix to strengthen structural stability of NiFe2O4 nanoparticles as high-capacity anodes of Li-ion batteries

Zhao, Taolin / Huang, Xiyun / Meng, Yu / Shen, Jiangang / Zheng, Yingdi / Chang, Liyao / Chen, Shaokang

Journal of materials science. 2022 Aug., v. 57, no. 31

2022

Abstract: Transition metal oxides are considered as promising anode materials of high-performance lithium-ion batteries because of their higher specific capacities than that of commercial graphite. However, they still suffer from huge volume expansion/contraction ... ...

Abstract	Transition metal oxides are considered as promising anode materials of high-performance lithium-ion batteries because of their higher specific capacities than that of commercial graphite. However, they still suffer from huge volume expansion/contraction during cycling, leading to fast decay of the reversible capacity and poor cycle stability. In this work, a graded porous carbon matrix has been in situ constructed successfully to strengthen structural stability of NiFe₂O₄ nanoparticles via a facile and green low-temperature combustion method. The calcination temperature has a significant effect on the purity and electrochemical performances of the final NiFe₂O₄/C composites. NiFe₂O₄/C prepared at 350 °C shows a high first discharge capacity of 1385.8 mAh g⁻¹ at 200 mA g⁻¹, excellent cycle stability, and good rate capability. This excellent electrochemical performance may be attributed to its favorable graded porous structure. The carbon matrix can effectively protect the NiFe₂O₄ nanoparticles, buffer the surface stress caused by volume expansion/contraction, and facilitate the transmission of electrons and Li⁺ ions. The symbiotic relationship between NiFe₂O₄ active nanoparticles and graded porous carbon matrix strengthens the structural stability of the electrode, which expands the way of designing high-performance electrode materials for secondary rechargeable batteries.
Keywords	anodes ; combustion ; electrochemistry ; graphene ; symbiosis ; temperature
Language	English
Dates of publication	2022-08
Size	p. 14979-14992.
Publishing place	Springer US
Document type	Article
ZDB-ID	2015305-3
ISSN	1573-4803 ; 0022-2461
ISSN (online)	1573-4803
ISSN	0022-2461
DOI	10.1007/s10853-022-07576-z
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Ischemic postconditioning for stroke treatment: current experimental advances and future directions.

Chen, Hansen / Shen, Jiangang / Zhao, Heng

Conditioning medicine

2020 Volume 3, Issue 2, Page(s) 104–115

Abstract: Ischemic postconditioning (IPostC) protects against brain injury induced by stroke and is a potential strategy for ischemic stroke treatment. Understanding its underlying mechanisms and potential hurdles is essential for clinical translation. In this ... ...

Abstract	Ischemic postconditioning (IPostC) protects against brain injury induced by stroke and is a potential strategy for ischemic stroke treatment. Understanding its underlying mechanisms and potential hurdles is essential for clinical translation. In this review article, we will summarize the current advances in IPostC for stroke treatment and the underlying protective mechanisms. Strong evidence suggests that IPostC reduces brain infarct size, attenuates blood-brain barrier (BBB) damage and brain edema, and improves neurological outcomes. IPostC also promotes neurogenesis and angiogenesis at the recovery phase of ischemic stroke. The protective mechanisms involve its effects on anti-oxidative stress, anti-inflammation, and anti-apoptosis. In addition, it regulates neurotransmitter receptors, ion channels, heat shock proteins (HSP) 40/70, as well as growth factors such as BDNF and VEGF. Furthermore, IPostC modulates several cell signaling pathways, including the PI3K/Akt, MAPK, NF-κB, and the Gluk2/PSD95/MLK3/MKK7/JNK3 pathways. We also discuss the potential hurdles for IPostC's clinical translation, including insufficient IPostC algorithm studies, such as therapeutic time windows and ischemia-reperfusion periods and cycles, as well as its long-term protection. In addition, future studies should address confounding factors such as age, sex, and pre-existing conditions such as hypertension and hyperglycemia before stroke onset. At last, the combination of IPostC with other treatments, such as tissue plasminogen activator (t-PA), merits further exploration.
Language	English
Publishing date	2020-05-05
Publishing country	United States
Document type	Journal Article
ISSN	2577-3240
ISSN (online)	2577-3240
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Article ; Online: Combination of matrix solid phase dispersion and response surface evaluation for simultaneous detections of multiple bioactive constituents of traditional Chinese medicine formula: Using Baoyuan Capsule as an example.

Du, Qiaohui / Deng, Ruixia / Gao, Chong / Shen, Jiangang

Journal of pharmaceutical and biomedical analysis

2020 Volume 190, Page(s) 113495

Abstract: Traditional Chinese Medicine (TCM) formulae contain abundant chemical ingredients, leading to the development of high quality TCM productions be difficult. With different pharmacokinetic and pharmacodynamic parameters of the chemical ingredients in a TCM ...

Abstract	Traditional Chinese Medicine (TCM) formulae contain abundant chemical ingredients, leading to the development of high quality TCM productions be difficult. With different pharmacokinetic and pharmacodynamic parameters of the chemical ingredients in a TCM formula, it is desirable to simultaneously identify multiple ingredients for accurate quality control. In the present study, we introduce a novel strategy for TCM formula quality assessment by using the combined methods of the extraction condition optimization, quantitative analyzation, and response surface evaluation. We used Bao-Yuan Capsule (BYC), a patented TCM production, as a model system for quality assessment. We applied matrix solid phase dispersion (MSPD) as a rapid and efficient method to prepare sample extraction. Q-Trap-MS related accurate methods were applied to simultaneously analyze 13 bioactive constituents as bioactive markers in the BYC. Those methods revealed a high sensitivity to detect the target compounds at the concentrations ranging from 0.12 to 0.95 ng/mL for flavonoids and ginsenosides whose recoveries were ranged from 91.93%-105.84%. We employed the response surface methodology to optimize the extraction conditions including dispersant/sample ratio, solvent concentration, and elution volume based on the content of ginsenosides as the test samples. The results showed the high extraction efficiency of ginsenosides with dispersant/sample ratio at 3/4, methanol concentration at 85 %, and elution volume at 15 mL. Taken together, we conclude that the combination strategy of MSPD and response surface evaluation for simultaneous detections of multiple bioactive constituents could be a powerful and efficient method for performing accurately quality control of complex TCM production preparations.
MeSH term(s)	Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal/analysis ; Flavonoids/analysis ; Medicine, Chinese Traditional ; Solid Phase Extraction ; Solvents
Chemical Substances	Drugs, Chinese Herbal ; Flavonoids ; Solvents
Language	English
Publishing date	2020-08-02
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2020.113495
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Article: Targeting Myeloperoxidase (MPO) Mediated Oxidative Stress and Inflammation for Reducing Brain Ischemia Injury: Potential Application of Natural Compounds.

Chen, Shuang / Chen, Hansen / Du, Qiaohui / Shen, Jiangang

Frontiers in physiology

2020 Volume 11, Page(s) 433

Abstract: Oxidative stress and inflammation are two critical pathological processes of cerebral ischemia-reperfusion injury. Myeloperoxidase (MPO) is a critical inflammatory enzyme and therapeutic target triggering both oxidative stress and neuroinflammation in ... ...

Abstract	Oxidative stress and inflammation are two critical pathological processes of cerebral ischemia-reperfusion injury. Myeloperoxidase (MPO) is a critical inflammatory enzyme and therapeutic target triggering both oxidative stress and neuroinflammation in the pathological process of cerebral ischemia-reperfusion injury. MPO is presented in infiltrated neutrophils, activated microglial cells, neurons, and astrocytes in the ischemic brain. Activation of MPO can catalyze the reaction of chloride and H
Language	English
Publishing date	2020-05-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2020.00433
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