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Article ; Online: The function of hyperpolarization-activated cyclic nucleotide-gated channel in diabetic cystopathy.

He, P / Zhou, X-Z / Shen, W-H / Zhang, H / Li, L-K / Wang, Y-Q

European review for medical and pharmacological sciences

2018 Volume 22, Issue 20, Page(s) 6575–6582

Abstract: Objective: We aimed at investigating changes in the expression and physiological function of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in interstitial cells of Cajal (ICC) in diabetic state.: Materials and methods: Twenty ... ...

Abstract	Objective: We aimed at investigating changes in the expression and physiological function of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in interstitial cells of Cajal (ICC) in diabetic state. Materials and methods: Twenty adult female Sprague-Dawley (SD) rats were randomly assigned to control and Zucker diabetic fatty (ZDF) group. The protein and mRNA expression of HCN isoforms and C-kit in the rat bladders were detected using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). The bladder contraction was evaluated using a bladder smooth muscle strip test. Whole cell patch-clamp techniques were used to detect the activity of HCN channels. Immunofluorescent staining was used to the positive expression of HCN and C-kit in ICC. Results: cAMP, as HCN channel-specific stimulant, could increase the frequency and amplitude of spontaneous contractions in both group, while cAMP inducing contraction of ZDF rats, was still significantly lower compared with the control group. Acute bladder ICCs were isolated by collagenase digestion. Classic Ih current pattern was recorded on ICCs while Ih current amplitude of ICCs from ZDF diabetic rats was significantly lower than the control group. The expression and mRNA of HCN1-4 isoforms in ZDF diabetic rats were both significantly lower compared with the control group. Meanwhile, the number of c-kit positive cells in ZDF diabetic rats showed no significant differences compared with controls. The morphological structure of ICC in the bladder of ZDF rats was relatively loose and the number of their cell process was apparently decreased. Conclusions: The structure of ICCs in ZDF rats was relatively loose, their connection to each other was also diminished. The expression of HCN was down-regulated and its response to cAMP was also decreased. HCN channels in bladder ICCs might regulate detrusor contraction. Changes in HCN expression and activity in bladder ICCs might be one of the most important mechanisms of diabetic cystopathy.
MeSH term(s)	Animals ; Diabetes Complications/metabolism ; Diabetes Complications/pathology ; Diabetes Complications/physiopathology ; Female ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels/genetics ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels/metabolism ; Interstitial Cells of Cajal/metabolism ; Interstitial Cells of Cajal/pathology ; Muscle Contraction ; Muscle, Smooth/metabolism ; Muscle, Smooth/pathology ; Muscle, Smooth/physiopathology ; Proto-Oncogene Proteins c-kit/genetics ; Proto-Oncogene Proteins c-kit/metabolism ; Rats, Sprague-Dawley ; Rats, Zucker ; Signal Transduction ; Urinary Bladder/metabolism ; Urinary Bladder/pathology ; Urinary Bladder/physiopathology ; Urinary Bladder Diseases/metabolism ; Urinary Bladder Diseases/pathology ; Urinary Bladder Diseases/physiopathology ; Urodynamics
Chemical Substances	Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ; Proto-Oncogene Proteins c-kit (EC 2.7.10.1)
Language	English
Publishing date	2018-11-06
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201810_16131
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Article: NtSET1, a member of a newly identified subgroup of plant SET-domain-containing proteins, is chromatin-associated and its ectopic overexpression inhibits tobacco plant growth.

Shen, W H

The Plant journal : for cell and molecular biology

2001 Volume 28, Issue 4, Page(s) 371–383

Abstract: The SET- and chromo-domains are recognized as signature motifs for proteins that contribute to epigenetic control of gene expression through effects on the regional organization of chromatin structure. This paper reports the identification of a novel ... ...

Abstract	The SET- and chromo-domains are recognized as signature motifs for proteins that contribute to epigenetic control of gene expression through effects on the regional organization of chromatin structure. This paper reports the identification of a novel subgroup of SET-domain-containing proteins in tobacco and Arabidopsis, which show highest homologies with the Drosophila position-effect-variegation repressor protein SU(VAR)3-9 and the yeast centromer silencing protein CLR4. The tobacco SET-domain-containing protein (NtSET1) was fused to the green fluorescence protein (GFP) that serves as a visual marker for localization of the recombinant protein in living cells. Whereas control GFP protein alone was uniformly dispersed within the nucleus and cytoplasm, the NtSET1-GFP fusion protein showed a non-uniform localization to multiple nuclear regions in interphase tobacco TBY2 cells. During mitosis, the NtSET1-GFP associated with condensed chromosomes with a non-random distribution. The NtSET1 thus appears to have distinct target regions in the plant chromatin. Overexpression of the NtSET1-GFP in transgenic tobacco inhibited plant growth, implicating the possible involvement of the NtSET1 in transcriptional repression of growth control genes through the formation of higher-order chromatin domains.
MeSH term(s)	Amino Acid Sequence ; Arabidopsis/genetics ; Base Sequence ; Cell Cycle ; Cell Cycle Proteins/genetics ; Chromatin/chemistry ; Growth Inhibitors ; Histone-Lysine N-Methyltransferase ; Methyltransferases/genetics ; Molecular Sequence Data ; Plant Proteins/genetics ; Plant Proteins/isolation & purification ; Protein Structure, Tertiary ; Recombinant Fusion Proteins/isolation & purification ; Schizosaccharomyces pombe Proteins ; Sequence Homology, Amino Acid ; Nicotiana/genetics
Chemical Substances	Cell Cycle Proteins ; Chromatin ; Growth Inhibitors ; Plant Proteins ; Recombinant Fusion Proteins ; SET1 protein, Nicotiana tabacum ; Schizosaccharomyces pombe Proteins ; Methyltransferases (EC 2.1.1.-) ; SU(VAR)3-9 (EC 2.1.1.-) ; Histone-Lysine N-Methyltransferase (EC 2.1.1.43) ; clr4 protein, S pombe (EC 2.1.1.43)
Language	English
Publishing date	2001-09-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1046/j.1365-313x.2001.01135.x
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Article ; Online: PTEN: a new guardian of the genome.

Yin, Y / Shen, W H

Oncogene

2008 Volume 27, Issue 41, Page(s) 5443–5453

Abstract: The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) tumor suppressor is a phosphatase that antagonizes the phosphoinositol-3-kinase/AKT signaling pathway and suppresses cell survival as well as cell proliferation. PTEN is the second most ... ...

Abstract	The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) tumor suppressor is a phosphatase that antagonizes the phosphoinositol-3-kinase/AKT signaling pathway and suppresses cell survival as well as cell proliferation. PTEN is the second most frequently mutated gene in human cancer after p53. Germline mutations of PTEN have been found in cancer susceptibility syndromes, such as Cowden syndrome, in which over 80% of patients have mutations of PTEN. Homozygous deletion of Pten causes embryonic lethality, suggesting that PTEN is essential for embryonic development. Mice heterozygous for Pten develop spontaneous tumors in a variety of organs comparable with the spectrum of its mutations in human cancer. The mechanisms of PTEN functions in tumor suppression are currently under intense investigation. Recent studies demonstrate that PTEN plays an essential role in the maintenance of chromosomal stability and that loss of PTEN leads to massive alterations of chromosomes. The tumor suppressor p53 is known as a guardian of the genome that mediates the cellular response to environmental stress, leading to cell cycle arrest or cell death. Through completely different mechanisms, PTEN also protects the genome from instability. Thus, we propose that PTEN is a new guardian of the genome. In this review, we will discuss new discoveries on the role of PTEN in tumor suppression and explore mechanisms by which PTEN maintains genomic stability.
MeSH term(s)	Animals ; Cell Nucleus/metabolism ; Gene Regulatory Networks/physiology ; Genome, Human/physiology ; Genomic Instability/genetics ; Humans ; Lipid Metabolism/genetics ; Lipid Metabolism/physiology ; Models, Biological ; PTEN Phosphohydrolase/chemistry ; PTEN Phosphohydrolase/genetics ; PTEN Phosphohydrolase/metabolism ; PTEN Phosphohydrolase/physiology ; Phosphoprotein Phosphatases/genetics ; Phosphoprotein Phosphatases/physiology ; Tumor Suppressor Proteins/physiology
Chemical Substances	Tumor Suppressor Proteins ; Phosphoprotein Phosphatases (EC 3.1.3.16) ; PTEN Phosphohydrolase (EC 3.1.3.67)
Language	English
Publishing date	2008-09-18
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	639046-8
ISSN	1476-5594 ; 0950-9232
ISSN (online)	1476-5594
ISSN	0950-9232
DOI	10.1038/onc.2008.241
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Article: The plant E2F-Rb pathway and epigenetic control

Shen, W.H

Trends in plant science. Nov 2002. v. 7 (11)

2002

Keywords	Arabidopsis thaliana ; plant development ; plant proteins ; DNA-binding proteins ; transcription factors ; gene expression ; epigenetics ; cell cycle ; biochemical pathways ; chromatin ; chemical constituents of plants ; transcription (genetics)
Language	English
Dates of publication	2002-11
Size	p. 505-510.
Document type	Article
ZDB-ID	1305448-x
ISSN	1878-4372 ; 1360-1385
ISSN (online)	1878-4372
ISSN	1360-1385
Database	NAL-Catalogue (AGRICOLA)

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Article: Fault diagnosis based on PCA for sensors of laboratorial wastewater treatment process

Tao, E.P / Shen, W.H / Liu, T.L / Chen, X.Q

Chemometrics and intelligent laboratory systems. 2013 Oct. 15, v. 128

2013

Abstract: This paper presents a PCA (principal component analysis)-based diagnostic approach, combining the principal component scores with the principal component loadings, to determine the fault location of sensors in a pilot-scale SBR (sequencing batch reactor ... ...

Abstract	This paper presents a PCA (principal component analysis)-based diagnostic approach, combining the principal component scores with the principal component loadings, to determine the fault location of sensors in a pilot-scale SBR (sequencing batch reactor activated sludge process) wastewater treatment process. The PCA diagnostic model is firstly built with the historical normal data, and the determination of fault location of sensors in wastewater treatment process is further achieved through the combination of the scores with the loadings of principal components. The study results reveal that PCA model can be used to detect faults; the loadings of principal components can well represent the contributions of variables to the principal components; and the scores of principal components give a clear indication of the faulty samples. The feasibility and effectiveness of the application of the combination of score plots with loading plots for sensor fault diagnosis in the wastewater treatment process are well demonstrated in the study.
Keywords	activated sludge ; models ; principal component analysis ; wastewater treatment
Language	English
Dates of publication	2013-1015
Size	p. 49-55.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	55877-1
ISSN	0169-7439
ISSN	0169-7439
DOI	10.1016/j.chemolab.2013.07.012
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Article: NtSET1, a member of a newly identified subgroup of plant SET-domain-containing proteins, is chromatin-associated and its ectopic overexpression inhibits tobacco plant growth

Shen, W.H

Plant journal : for cell and molecular biology. Nov 2001. v. 28 (4)

2001

Abstract	The SET- and chromo-domains are recognized as signature motifs for proteins that contribute to epigenetic control of gene expression through effects on the regional organization of chromatin structure. This paper reports the identification of a novel subgroup of SET-domain-containing proteins in tobacco and Arabidopsis, which show highest homologies with the Drosophila position effect variegation repressor protein SU(VAR)3-9 and the yeast centromer silencing protein CLR4. The tobacco SET-domain-containing protein (NtSET1) was fused to the green fluorescence protein (GFP) that serves as a visual marker for localization of the recombinant protein in living cells. Whereas control GFP protein alone was uniformly dispersed within the nucleus and cytoplasm, the NtSET1-GFP fusion protein showed a non-uniform localization to multiple nuclear regions in interphase tobacco TBY2 cells. During mitosis, the NtSET1-GFP associated with condensed chromosomes with a non-random distribution. The NtSET1 thus appears to have distinct target regions in the plant chromatin. Overexpression of the NtSET1-GFP in transgenic tobacco inhibited plant growth, implicating the possible involvement of the NtSET1 in transcriptional repression of growth control genes through the formation of higher-order chromatin domains.
Keywords	Nicotiana tabacum ; plant proteins ; chromatin ; gene expression ; amino acid sequences ; animal proteins ; biomarkers ; cytoplasm ; chromosomes ; nucleotide sequences ; green fluorescent protein
Language	English
Dates of publication	2001-11
Size	p. 371-383.
Document type	Article
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
Database	NAL-Catalogue (AGRICOLA)

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Article: The plant cell cycle: G1/S regulation

Shen, W.H

Euphytica : Netherlands journal of plant breeding. 2001. v. 118 (2)

2001

Keywords	plants ; cells ; growth regulators ; proteolysis ; cell division ; cell differentiation ; biochemical pathways ; phosphorylation ; protein metabolism ; enzyme activity ; ubiquitin
Language	English
Size	p. 223-232.
Document type	Article
Note	In the special issue: Genetics--Better life for all / edited by S. Mohain Jain, B.S. Ahloowalia, G.S. Khush and Lihuang Zhu. Paper presented at a congress held August 10-15, 1998, Beijing, China.
ZDB-ID	216568-5
ISSN	0014-2336
ISSN	0014-2336
Database	NAL-Catalogue (AGRICOLA)

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Article: [Treatment of trigeminal neuralgia with cryotherapy: A preliminary evaluation in 27 cases]

Shen, W H

Shanghai kou qiang yi xue = Shanghai journal of stomatology

1993 Volume 2, Issue 4, Page(s) 199

Language	Chinese
Publishing date	1993-12
Publishing country	China
Document type	Journal Article
ZDB-ID	2269714-7
ISSN	1006-7248
ISSN	1006-7248
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Article: Fluorescence lifetimes of the tryptophan residues in ornithine transcarbamoylase.

Shen, W H

Biochemistry

1993 Volume 32, Issue 50, Page(s) 13925–13932

Abstract: Multifrequency (2-230 MHz) phase-modulation fluorescence measurements and site-directed mutagenesis have been employed to assign fluorescence lifetimes, quantum yields, and emission maxima to the four tryptophans in the enzyme ornithine transcarbamoylase ...

Abstract	Multifrequency (2-230 MHz) phase-modulation fluorescence measurements and site-directed mutagenesis have been employed to assign fluorescence lifetimes, quantum yields, and emission maxima to the four tryptophans in the enzyme ornithine transcarbamoylase from Escherichia coli (OTCase) (Trp-125, -92, -233, and -243). OTCase displays two apparent fluorescence lifetimes, 7.2 and 3.2 ns. Results on specific mutants show that Trp-233 has a lifetime of 7.1 ns, while TRP-125, -192, and -243 have lifetimes of 4.0, 3.6, and 4.9 ns, respectively. Thus, the specific conformational changes of the polypeptide segment involving Trp-233 may be monitored conveniently in the wild-type enzyme. On the basis of quantum yield values, Trp-233 is calculated to contribute approximately 43% of the fluorescence intensity of the enzyme, while direct measurements of the enzyme show that up to 65% of the total intensity is really emitted by this tryptophan. The discrepancy may arise from energy transfer from Trp-125 to Trp-233, with an efficiency of 20%. Application of the assigned tryptophan lifetimes to probe ligand-induced protein conformational changes has also been demonstrated.
MeSH term(s)	Amino Acid Sequence ; Chromatography, Gel ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli/enzymology ; Kinetics ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Ornithine Carbamoyltransferase/chemistry ; Ornithine Carbamoyltransferase/genetics ; Ornithine Carbamoyltransferase/isolation & purification ; Protein Conformation ; Spectrometry, Fluorescence ; Tryptophan/chemistry
Chemical Substances	Tryptophan (8DUH1N11BX) ; Ornithine Carbamoyltransferase (EC 2.1.3.3)
Language	English
Publishing date	1993-12-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1108-3
ISSN	1520-4995 ; 0006-2960
ISSN (online)	1520-4995
ISSN	0006-2960
DOI	10.1021/bi00213a023
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Article ; Online: Development and characterization of microsatellite loci in Excentrodendron hsienmu (Chun & How) H.T. Chang& R.H. Miau.

Shen, W H / Li, Z H / Peng, Y H / Yang, M H / Tan, Z Q / Zheng, W

Genetics and molecular research : GMR

2016 Volume 15, Issue 3

Abstract: Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were ... ...

Abstract	Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were characterized in E. hsienmu. The number of alleles per locus ranged from 2 to 9, with an average of 5.27. The expected heterozygosity value ranged from 0.053 to 0.780, with an average of 0.568 and the observed heterozygosity value ranged from 0 to 0.595, with an average of 0.268. The polymorphic information content value ranged from 0.051 to 0.740, with an average of 0.521. These newly designed markers will be of great potential significance and profound influence in future research related to the genetic diversity, population structure, and patterns of gene flow of this species, which will contribute to the implementation of conservation and management strategies for this species.
MeSH term(s)	Alleles ; DNA, Plant/genetics ; Endangered Species ; Genetic Loci ; Genetic Variation ; Heterozygote ; Microsatellite Repeats ; Polymorphism, Genetic ; Tiliaceae/genetics
Chemical Substances	DNA, Plant
Language	English
Publishing date	2016-08-19
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/gmr.15038060
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