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Article ; Online: RNA

Chen, Yan-Hua / Jiang, Tao / Yasen, Ayinuer / Fan, Bing-Yan / Zhu, Juan / Wang, Mei-Xian / Shen, Xing-Jia

2023 Volume 113, Issue 5, Page(s) 665–675

Abstract: Environment-induced epigenetics are involved in diapause regulation, but the molecular mechanism that epigenetically couples nutrient metabolism to diapause regulation remains unclear. In this study, we paid special attention to the significant ... ...

Abstract	Environment-induced epigenetics are involved in diapause regulation, but the molecular mechanism that epigenetically couples nutrient metabolism to diapause regulation remains unclear. In this study, we paid special attention to the significant differences in the level of
MeSH term(s)	Female ; Animals ; Bombyx/genetics ; Diapause, Insect/genetics ; Phosphatidate Phosphatase/metabolism ; RNA/metabolism ; Lipid Metabolism ; Diapause ; Adenosine/metabolism ; Ovum ; Insect Proteins/genetics ; Insect Proteins/metabolism
Chemical Substances	Phosphatidate Phosphatase (EC 3.1.3.4) ; RNA (63231-63-0) ; Adenosine (K72T3FS567) ; Insect Proteins
Language	English
Publishing date	2023-08-09
Publishing country	England
Document type	Journal Article
ZDB-ID	280516-9
ISSN	1475-2670 ; 0007-4853
ISSN (online)	1475-2670
ISSN	0007-4853
DOI	10.1017/S0007485323000330
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Article ; Online: m

Chen, Yan-Hua / Jiang, Tao / Yasen, Ayinuer / Fan, Bing-Yan / Zhu, Juan / Wang, Mei-Xian / Qian, Ping / Shen, Xing-Jia

Molecular biology reports

2023 Volume 50, Issue 6, Page(s) 5295–5306

Abstract: Background: Research has shown that epigenetic modification are involved the regulation of diapause in bivoltine silkworms (Bombyx mori), but it remains unclear how epigenetic modification in response to environmental signals precisely to regulate the ... ...

Abstract	Background: Research has shown that epigenetic modification are involved the regulation of diapause in bivoltine silkworms (Bombyx mori), but it remains unclear how epigenetic modification in response to environmental signals precisely to regulate the diapause processing of bivoltine B. mori. Methods and results: In this study, the diapause terminated eggs of bivoltine B. mori, Qiufeng (QF) were divided into two groups: a QFHT group incubated at 25 °C with a natural day/night cycle to produce diapause eggs, and a QFLT group incubated at 16.5 °C in darkness to produce non-diapause eggs. On the 3rd day of the pupal stage, the total RNAs of the eggs were extracted and their N6-adenosine methylation (m Conclusions: m
MeSH term(s)	Animals ; Female ; Bombyx/genetics ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Signal Transduction ; Juvenile Hormones/metabolism ; Ovum/metabolism
Chemical Substances	mevalonate kinase (EC 2.7.1.36) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-) ; Juvenile Hormones
Language	English
Publishing date	2023-05-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-023-08489-z
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Article: [Advances in m

Xue, Peng / Jiang, Tao / Shen, Xing Jia

Yi chuan = Hereditas

2019 Volume 41, Issue 5, Page(s) 404–412

Abstract: ... ...

Abstract	N
MeSH term(s)	Adenosine/analogs & derivatives ; Adenosine/chemistry ; Epigenesis, Genetic ; RNA, Messenger/chemistry ; RNA, Messenger/genetics ; Virus Replication ; Viruses
Chemical Substances	RNA, Messenger ; N-methyladenosine (CLE6G00625) ; Adenosine (K72T3FS567)
Language	Chinese
Publishing date	2019-04-26
Publishing country	China
Document type	Journal Article ; Review
ISSN	0253-9772
ISSN	0253-9772
DOI	10.16288/j.yczz.19-042
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Article ; Online: Cordyceps cicadae polysaccharides inhibit human cervical cancer hela cells proliferation via apoptosis and cell cycle arrest.

Xu, Jiao / Tan, Zhi-Cheng / Shen, Zhong-Yuan / Shen, Xing-Jia / Tang, Shun-Ming

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2021 Volume 148, Page(s) 111971

Abstract: The present study presented the extraction and purification of polysaccharides from artificially cultured Cordyceps cicadae and wild Cordyceps cicadae by pre-soaking ultrasonic water extraction. The effects of different concentrations of polysaccharides ... ...

Abstract	The present study presented the extraction and purification of polysaccharides from artificially cultured Cordyceps cicadae and wild Cordyceps cicadae by pre-soaking ultrasonic water extraction. The effects of different concentrations of polysaccharides on proliferation and cytotoxicity of Hela cells were detected by MTT and LDH methods. The results showed that the proliferation of Hela cells was inhibited by polysaccharides treatment (25 μg/mL-1600 μg/mL). The results of flow cytometry further confirmed that polysaccharides blocked the cell cycle in the S phase and promoted apoptosis. RT-qPCR and Western Blot were used to study the mRNA and protein expression of genes related to cell cycle and apoptosis signaling pathway. The results showed that polysaccharides treatment inhibited the expression of Cyclin E, Cyclin A and CDK2 and up regulated the expression of P53. Further, activation of Caspase cascade reaction, up regulation of death receptor, and the ratio of pro-apoptotic factor/anti-apoptotic factors, thus caused the cell cycle arrest and induced the apoptosis. The above research results lay a foundation for extending the anti-cancer effects of natural plant resources with low toxicity and high efficiency.
MeSH term(s)	Antineoplastic Agents/isolation & purification ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Cordyceps/chemistry ; Drug Screening Assays, Antitumor ; Gene Expression Regulation, Neoplastic/drug effects ; HeLa Cells ; Humans ; Mitochondria/metabolism ; Polysaccharides/isolation & purification ; Polysaccharides/pharmacology ; S Phase Cell Cycle Checkpoints/drug effects
Chemical Substances	Antineoplastic Agents ; Polysaccharides
Language	English
Publishing date	2021-01-06
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2021.111971
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Article ; Online: A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx mori.

Zhu, Juan / Chen, Yan-Rong / Geng, Tao / Tang, Shun-Ming / Zhao, Qiao-Ling / Shen, Xing-Jia

Gene

2021 Volume 777, Page(s) 145450

Abstract: The Bombyx mori Shadow gene (BmShadow) belongs to the superfamily of cytochrome P450 genes. To elucidate the function of the BmShadow gene and its association with diapause, we employed the CRISPR/Cas9 system to knock out the BmShadow gene in the ... ...

Abstract	The Bombyx mori Shadow gene (BmShadow) belongs to the superfamily of cytochrome P450 genes. To elucidate the function of the BmShadow gene and its association with diapause, we employed the CRISPR/Cas9 system to knock out the BmShadow gene in the bivoltine strain Qiufeng. The mutant (BmShadow
MeSH term(s)	Amino Acids/metabolism ; Animals ; Bombyx/genetics ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Ecdysterone/genetics ; Ecdysterone/metabolism ; Insect Proteins/genetics ; Larva/genetics ; Molting/genetics
Chemical Substances	Amino Acids ; Drosophila Proteins ; Insect Proteins ; Ecdysterone (5289-74-7) ; Cytochrome P-450 Enzyme System (9035-51-2) ; sad protein, Drosophila (EC 1.13.-)
Language	English
Publishing date	2021-01-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2021.145450
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Article: A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx mori

Zhu, Juan / Chen, Yan-Rong / Geng, Tao / Tang, Shun-Ming / Zhao, Qiao-ling / Shen, Xing-Jia

Gene. 2021 Apr. 20, v. 777

2021

Abstract	The Bombyx mori Shadow gene (BmShadow) belongs to the superfamily of cytochrome P450 genes. To elucidate the function of the BmShadow gene and its association with diapause, we employed the CRISPR/Cas9 system to knock out the BmShadow gene in the bivoltine strain Qiufeng. The mutant (BmShadow⁻/⁻) was obtained in G₂, exhibiting a 42-base deletion corresponded exactly to the amino acids regions from positions 155 to 168. The larvae of BmShadow⁻/⁻ cannot moult at the pre-moulting stage of the 2nd instar. When the BmShadow⁻/⁻ larvae were fed with 20E analogue at the late stage of the 2nd instar, they were rescued and developed into the 3rd instar. Rescue experiments indicated that the 20E concentration of BmShadow⁻/⁻ larvae was significantly lower than that in WT larvae, and the 20E concentration of BmShadow⁻/⁻ larvae which fed 20E analogue was restored to normal levels. Interestingly, the BmShadow⁻/⁻ larvae could not moult on the 1st instar when they hatched from eggs after being stored at 5 °C for 40 days or after hibernation, suggesting that the 20E transported from the mother was partially consumed in the diapause maintenance phase. Our study confirmed that BmShadow is involved in 20E synthesis and a 14-amino acids region from position 155 to 168 was essential for its function, also there appears to be no other compensation pathway in vivo, which offered an important potential target locus for the control of silkworm development and the biological control of agricultural and forestry pests.
Keywords	Bombyx mori ; CRISPR-Cas systems ; biological control ; cytochrome P-450 ; diapause ; forestry ; genes ; hibernation ; instars ; loci ; molting ; mutants ; silkworms
Language	English
Dates of publication	2021-0420
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2021.145450
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Article ; Online: Exorista sorbillans (Diptera: Tachinidae) parasitism shortens host larvae growth duration by regulating ecdysone and juvenile hormone titers in Bombyx mori (Lepidoptera: Bombycidae).

Wang, Shan-Shan / Wang, Lei-Lei / Pu, Yue-Xia / Liu, Ji-Yin / Wang, Mei-Xian / Zhu, Juan / Shen, Zhong-Yuan / Shen, Xing-Jia / Tang, Shun-Ming

Journal of insect science (Online)

2023 Volume 23, Issue 3

Abstract: The tachinid fly, Exorista sorbillans, is a notorious ovolarviparous endoparasitoid of the silkworm, Bombyx mori, causing severe damage to silkworm cocoon industry. Silkworm larvae show typically precocious wandering behavior after being parasitized by E. ...

Abstract	The tachinid fly, Exorista sorbillans, is a notorious ovolarviparous endoparasitoid of the silkworm, Bombyx mori, causing severe damage to silkworm cocoon industry. Silkworm larvae show typically precocious wandering behavior after being parasitized by E. sorbillans; however, the underlying molecular mechanism remains unexplored. Herein, we investigated the changes in the levels of 20-hydroxyecdysone (20E) and juvenile hormone (JH) titer, and they both increased in the hemolymph of parasitized silkworms. Furthermore, we verified the expression patterns of related genes, which showed an upregulation of 20E signaling and biosynthesis genes but a significant downregulation of ecdysone oxidase (EO), a 20E inactivation enzyme, in parasitized silkworms. In addition, related genes of the JH signaling were activated in parasitized silkworms, while related genes of the JH degradation pathway were suppressed, resulting in an increase in JH titer. Notably, the precocious wandering behavior of parasitized silkworms was partly recoverable by silencing the transcriptions of BmCYP302A1 or BmCYP307A1 genes. Our findings suggest that the developmental duration of silkworm post parasitism could be shortened by regulation of 20E and JH titers, which may help silkworm to resist the E. sorbillans infestation. These findings provide a basis for deeper insight into the interplay between silkworms and E. sorbillans and may serve as a reference for the development of a novel approach to control silkworm myiasis.
MeSH term(s)	Animals ; Bombyx ; Diptera/metabolism ; Larva ; Ecdysone/metabolism ; Lepidoptera/metabolism ; Juvenile Hormones/metabolism ; Manduca
Chemical Substances	Ecdysone (3604-87-3) ; Juvenile Hormones
Language	English
Publishing date	2023-06-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2049098-7
ISSN	1536-2442 ; 1536-2442
ISSN (online)	1536-2442
ISSN	1536-2442
DOI	10.1093/jisesa/iead034
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Article ; Online: Bombyx mori miR-2845 represses the expression of fibroin light chain gene both in vitro and in vivo.

Huang, JingYi / Chen, YanHua / Zhu, Juan / Wang, MeiXian / Tang, ShunMing / Yi, YongZhu / Shen, XingJia

PloS one

2021 Volume 16, Issue 12, Page(s) e0261391

Abstract: To study the regulatory function of Bombyx mori (B. mori) miRNAs (bmo-miR) on the expression of fibroin light chain gene (BmFib-L), the 3'UTR of BmFib-L mRNA was used as the target for online prediction of miRNAs from miRBase using RNAhybrid Software, ... ...

Abstract	To study the regulatory function of Bombyx mori (B. mori) miRNAs (bmo-miR) on the expression of fibroin light chain gene (BmFib-L), the 3'UTR of BmFib-L mRNA was used as the target for online prediction of miRNAs from miRBase using RNAhybrid Software, and miR-2845 was screened out. First, the expression profiles of miR-2845 and BmFib-L in larvae of the 5th instar were analyzed by Real-time quantitative PCR (RT-qPCR). Then recombinant plasmids (pcDNA3.0-pre-miR-2845 and pGL3.0-BmFib-L) were constructed to use for the expression of miR-2845 and BmFib-L 3'UTR, respectively. Cellular-level functional verification of miR-2845 on BmFib-L was carried out using multiple experimental methods (including dual luciferase reporter vectors, artificially synthesized mimics and inhibitors, and target site mutations). Finally, in vivo functional verification was performed by injecting the recombinant vector in 5th instar larvae. BmFib-L expression levels were detected using RT-qPCR in the posterior silk glands (PSG) of the injected larvae. Results showed that the expression of miR-2845 increased between the 1st and 5th day in 5th instar larvae, but began to decline on the 5th day, while the expression of the target gene BmFib-L increased sharply. This suggests that miR-2845 and BmFib-L expression levels show opposing trends, implying a negative regulatory relationship. In BmN cells, miR-2845 significantly down-regulated the expression of BmFib-L; the inhibitory effect of miR-2845 on BmFib-L was disappeared after mutation of the targeting site on 3'UTR of BmFib-L; in individuals, miR-2845 significantly down-regulated BmFib-L expression levels. Our results provide new experimental data for clarifying the molecular regulation mechanism of silk protein expression.
MeSH term(s)	3' Untranslated Regions ; Animals ; Bombyx/genetics ; China ; Computational Biology/methods ; Fibroins/genetics ; Fibroins/metabolism ; Gene Expression/genetics ; Gene Expression Regulation/genetics ; Insect Proteins/genetics ; Insect Proteins/metabolism ; Larva/genetics ; MicroRNAs/genetics ; Transcription Factors/metabolism
Chemical Substances	3' Untranslated Regions ; Insect Proteins ; L-chain, fibroin protein, insect ; MicroRNAs ; Transcription Factors ; fibroin, silkworm ; Fibroins (9007-76-5)
Language	English
Publishing date	2021-12-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0261391
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Article ; Online: Overview of research on Bombyx mori microRNA.

Wang, Xin / Tang, Shun-ming / Shen, Xing-jia

Journal of insect science (Online)

2014 Volume 14, Page(s) 133

Abstract: MicroRNAs (miRNAs) constitute some of the most significant regulatory factors involved at the post-transcriptional level after gene expression, contributing to the modulation of a large number of physiological processes such as development, metabolism, ... ...

Abstract	MicroRNAs (miRNAs) constitute some of the most significant regulatory factors involved at the post-transcriptional level after gene expression, contributing to the modulation of a large number of physiological processes such as development, metabolism, and disease occurrence. This review comprehensively and retrospectively explores the literature investigating silkworm, Bombyx mori L. (Lepidoptera: Bombicidae), miRNAs published to date, including discovery, identification, expression profiling analysis, target gene prediction, and the functional analysis of both miRNAs and their targets. It may provide experimental considerations and approaches for future study of miRNAs and benefit elucidation of the mechanisms of miRNAs involved in silkworm developmental processes and intracellular activities of other unknown non-coding RNAs.
MeSH term(s)	Animals ; Bombyx/genetics ; Bombyx/growth & development ; Bombyx/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; MicroRNAs
Chemical Substances	MicroRNAs
Language	English
Publishing date	2014-10-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2049098-7
ISSN	1536-2442 ; 1536-2442
ISSN (online)	1536-2442
ISSN	1536-2442
DOI	10.1093/jis/14.1.133
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Article: Biological functions of microRNAs.

Huang, Yong / Shen, Xing Jia / Zou, Quan / Zhao, Qiao Ling

Bioorganicheskaia khimiia

2011 Volume 36, Issue 6, Page(s) 747–752

Abstract: The small regulatory non-coding RNA molecules, known as microRNAs (miRNAs), have been recognized as potential regulator of gene expression and modulate the gene function at the post-transcriptional level. It is now clear that miRNA biogenesis and ... ...

Abstract	The small regulatory non-coding RNA molecules, known as microRNAs (miRNAs), have been recognized as potential regulator of gene expression and modulate the gene function at the post-transcriptional level. It is now clear that miRNA biogenesis and function are related to the molecular mechanisms of various clinical diseases, which can potentially regulate every aspect of cellular activity, including differentiation and development, metabolism, proliferation, apoptotic cell death viral infection and tumorgenesis. Here, we review recent work and provide insight into the diverse roles of miRNAs.
MeSH term(s)	Animals ; Apoptosis/genetics ; Cell Differentiation/genetics ; Cell Proliferation ; Cell Transformation, Neoplastic/genetics ; Cell Transformation, Neoplastic/metabolism ; Gene Expression Regulation ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Viruses/genetics ; Viruses/metabolism
Chemical Substances	MicroRNAs
Language	English
Publishing date	2011-02-03
Publishing country	Russia (Federation)
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	194993-7
ISSN	0132-3423
ISSN	0132-3423
DOI	10.1134/s1068162010060026
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