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Article ; Online: Protective efficacy of

Sun, Hong-Chao / Deng, Pu-Ming / Fu, Yuan / Deng, Jin-Hua / Xie, Rong-Hui / Huang, Jing / Qi, Meng / Shi, Tuan-Yuan

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1209755

Abstract: Background: Toxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses.: Methods: In the ... ...

Abstract	Background: Toxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses. Methods: In the current study, poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles were used as a delivery system for the T. gondii dense granule antigens GRA12 and GRA7. BALB/c mice were injected with the vaccines and protective efficacy was evaluated. Results: Mice immunized with PLGA+GRA12 exhibited significantly higher IgG, and a noticeable predominance of IgG2a over IgG1 was also observed. There was a 1.5-fold higher level of lymphocyte proliferation in PLGA+GRA12-injected mice compared to Alum+GRA12-immunized mice. Higher levels of IFN-g and IL-10 and a lower level of IL-4 were detected, indicating that Th1 and Th2 immune responses were induced but the predominant response was Th1. There were no significant differences between Alum+GRA7-immunized and PLGA+GRA7-immunized groups. Immunization with these four vaccines resulted in significantly reduced parasite loads, but they were lowest in PLGA+GRA12-immunized mice. The survival times of mice immunized with PLGA+GRA12 were also significantly longer than those of mice in the other vaccinated groups. Conclusion: The current study indicated that T. gondii GRA12 recombinant protein encapsulated in PLGA nanoparticles is a promising vaccine against acute toxoplasmosis, but PLGA is almost useless for enhancing the immune response induced by T. gondii GRA7 recombinant protein.
MeSH term(s)	Humans ; Animals ; Mice ; Toxoplasma ; Protozoan Proteins/genetics ; Antigens, Protozoan/genetics ; Toxoplasmosis ; Recombinant Proteins ; Lactic Acid ; Nanoparticles ; Mice, Inbred BALB C ; Protozoan Vaccines ; Antibodies, Protozoan
Chemical Substances	aluminum sulfate (34S289N54E) ; Protozoan Proteins ; Antigens, Protozoan ; Recombinant Proteins ; Lactic Acid (33X04XA5AT) ; Protozoan Vaccines ; Antibodies, Protozoan
Language	English
Publishing date	2023-07-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1209755
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Article ; Online: Molecular characterization and protective efficacy of vacuolar protein sorting 29 from

Shi, Tuan-Yuan / Li, Tian-En / Hao, Yun / Sun, Hong-Chao / Fu, Yuan / Yan, Wen-Chao / Hao, Li-Li

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1205782

Abstract: Introduction: Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and is essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 of : Methods: Here, we ... ...

Abstract	Introduction: Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and is essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 of Methods: Here, we cloned and prokaryotically expressed a partial sequence of Results: An EtVPS29 gene fragment with an ORF reading frame of 549 bp was cloned. The band size of the expressed recombinant protein, rEtVPS29, was approximately 39 kDa and was recognized by the chicken anti- Discussion: These results suggest that rEtVPS29 can induce a specific immune response and is a potential candidate for the development of novel vaccines against
MeSH term(s)	Animals ; Eimeria tenella/genetics ; Chickens ; Recombinant Proteins/metabolism ; Immunization ; Vaccination/veterinary ; Oocysts/metabolism ; Sporozoites ; Poultry Diseases/prevention & control ; Protozoan Vaccines/genetics
Chemical Substances	Recombinant Proteins ; Protozoan Vaccines
Language	English
Publishing date	2023-07-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1205782
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Article ; Online: Dominant infection of Cryptosporidium baileyi in broiler chickens in Zhejiang Province, China.

Feng, Xinwei / Deng, Jinhua / Zhang, Zhenjie / Yu, Fuchang / Zhang, Jianing / Shi, Tuanyuan / Sun, Hongchao / Qi, Meng / Liu, Xuehan

Parasitology research

2023 Volume 122, Issue 9, Page(s) 1993–2000

Abstract: Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. ... ...

Abstract	Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate of Cryptosporidium was 6.3% (51/812), and five of 11 farms were Cryptosporidium positive. Broilers aged > 90 days accounted for the highest infection rate of 16.1% (6/56), followed by those aged 30-60 days (10.6%, 38/358) and 60-90 days (4/378, 1.1%). Two Cryptosporidium species were identified by sequence analysis, with the predominant species being C. baileyi (96.1%, 49/51) and the minor infection being C. meleagridis (3.9%, 2/51). Based on the 60-kDa glycoprotein (gp60) gene, two C. meleagridis-positive isolates were identified as one known subtype, IIIbA24G1R1. This study indicated the common occurrence of C. baileyi in broiler chickens in this region and low zoonotic transmission potential of Cryptosporidium to humans.
MeSH term(s)	Humans ; Animals ; Cryptosporidium ; Chickens/parasitology ; Cryptosporidiosis/parasitology ; Poultry Diseases/parasitology ; China/epidemiology ; Feces/parasitology ; Genotype
Language	English
Publishing date	2023-06-22
Publishing country	Germany
Document type	Journal Article
ZDB-ID	284966-5
ISSN	1432-1955 ; 0932-0113 ; 0044-3255
ISSN (online)	1432-1955
ISSN	0932-0113 ; 0044-3255
DOI	10.1007/s00436-023-07898-0
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Article ; Online: Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China.

Sun, Hongchao / Su, Xinyao / Fu, Yuan / Hao, Lili / Zhou, Wei / Zhou, Zhijin / Huang, Jing / Wang, Yimin / Shi, Tuanyuan

Poultry science

2023 Volume 102, Issue 8, Page(s) 102845

Abstract: Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella ... ...

Abstract	Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella varies among geographic strains. At present, there are no reports related to the pathogenicity and drug resistance of E. tenella in Yiwu, Zhejiang province, China. A total of 600 fecal samples were collected from 10 farms in Zhejiang province, the overall oocyst prevalence was 54.2% (325/600). The prevalence was significantly higher (P < 0.01) in chickens under 40 d (97.5%) than that in chickens between 60 and 85-days-old (40.5%) and chickens over 90-days-old (24.5%). E. tenella stain was isolated from fecal samples of chickens in Yiwu and the pathogenicity of this isolate was determined, and then we recorded the survival rate, bloody stool score, lesion score, average weight gain. The results showed that all of the chickens infected with 5 × 10
MeSH term(s)	Animals ; Eimeria tenella ; Clopidol ; Nicarbazin ; Virulence ; Chickens ; Coccidiosis/epidemiology ; Coccidiosis/veterinary ; Weight Gain ; Drug Resistance ; Poultry Diseases/epidemiology
Chemical Substances	sulfachlorpyrazine (96R84UZ2XA) ; Clopidol (8J763HFF5N) ; Nicarbazin (11P9NUA12U)
Language	English
Publishing date	2023-06-05
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.102845
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Article ; Online: Enhancing Immune Responses to a DNA Vaccine Encoding

Sun, Hong-Chao / Huang, Jing / Fu, Yuan / Hao, Li-Li / Liu, Xin / Shi, Tuan-Yuan

Frontiers in cellular and infection microbiology

2021 Volume 11, Page(s) 787635

Abstract: Toxoplasma ... ...

Abstract	Toxoplasma gondii
MeSH term(s)	Animals ; Antibodies, Protozoan ; Antigens, Protozoan/genetics ; Calcium Phosphates ; Immunity, Humoral ; Mice ; Mice, Inbred BALB C ; Nanoparticles ; Protozoan Proteins/genetics ; Protozoan Vaccines ; Toxoplasma/genetics ; Vaccines, DNA
Chemical Substances	Antibodies, Protozoan ; Antigens, Protozoan ; Calcium Phosphates ; Protozoan Proteins ; Protozoan Vaccines ; Vaccines, DNA
Language	English
Publishing date	2021-12-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2021.787635
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Article: Molecular Detection and Genotyping of Toxoplasma gondii from Pigs for Human Consumption in Zhejiang and Jiangsu Provinces, Eastern China

Sun, Hong-Chao / Fu, Yuan / Yuan, Xiu-Fang / Li, Jun-Xing / Xu, Li-hua / Zhang, Jia-Ning / Yu, Bin / Huang, Jing / Qi, Meng / Shi, Tuan-Yuan

Foodborne pathogens & disease. 2022 Sept. 30,

2022

Abstract: Toxoplasma gondii infections are common in humans and animals worldwide. Ingestion of raw or undercooked meat containing tissue cysts of T. gondii is one major source of transmission of this parasite. It is important to guarantee the meat quality of ... ...

Abstract	Toxoplasma gondii infections are common in humans and animals worldwide. Ingestion of raw or undercooked meat containing tissue cysts of T. gondii is one major source of transmission of this parasite. It is important to guarantee the meat quality of China since our pork industry produces about half of the world's pork. In this study, a total of 746 pig samples were collected from Zhejiang and Jiangsu provinces in eastern China, and examined for T. gondii infection by PCR amplification targeting B1 gene. In this study, we found that 57 of 746 (7.6%) pigs were positive for B1 gene, with 8.5% (48/562) in Zhejiang province and 4.9% (9/184) in Jiangsu province, respectively. The positive DNA samples were further genotyped at 11 genetic markers, including SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico through PCR–restriction fragment length polymorphism (PCR-RFLP) technology. Two genotypes (ToxoDB 9 and ToxoDB 10) of T. gondii were identified by PCR-RFLP in Zhejiang province. However, both genotypes were not determined from Jiangsu province, which is speculated on the low DNA concentration and the small number of samples. These results indicate that T. gondii infection is endemic in pigs in eastern China and may raise public food safety concerns, suggesting more interventions for T. gondii-related risks are needed in the future.
Keywords	DNA ; Toxoplasma gondii ; food safety ; genes ; genotyping ; humans ; ingestion ; loci ; meat quality ; parasites ; pork ; pork industry ; swine ; China
Language	English
Dates of publication	2022-0930
Publishing place	Mary Ann Liebert, Inc.
Document type	Article
ZDB-ID	2148479-X
ISSN	1556-7125 ; 1535-3141
ISSN (online)	1556-7125
ISSN	1535-3141
DOI	10.1089/fpd.2022.0036
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Article ; Online: A rhoptry protein, localizing in the bulb region of rhoptries, could induce protective immunity against

Shi, Tuan-Yuan / Zhou, Si-Han / Kong, Ya-Ru / Fu, Yuan / Liu, Yan / Yan, Wen-Chao / Zhou, Yong-Xue / Zhang, Liang / Hao, Li-Li / Sun, Hong-Chao

Frontiers in immunology

2023 Volume 14, Page(s) 1277955

Abstract: Background: Rhoptry organelle proteins (ROPs) secreted by apicomplexan parasites play important roles during parasites invasion and survival in host cells, and are potential vaccine candidates against apicomplexan diseases. : Methods: In this study, ... ...

Abstract	Background: Rhoptry organelle proteins (ROPs) secreted by apicomplexan parasites play important roles during parasites invasion and survival in host cells, and are potential vaccine candidates against apicomplexan diseases. Methods: In this study, an Results: An Conclusion: These results indicate that r
MeSH term(s)	Animals ; Mice ; Eimeria tenella ; Protozoan Proteins ; Coccidiosis/prevention & control ; Coccidiosis/veterinary ; Chickens ; Recombinant Proteins ; Sporozoites ; Oocysts/metabolism
Chemical Substances	Protozoan Proteins ; Recombinant Proteins
Language	English
Publishing date	2023-12-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1277955
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Article ; Online: Transcriptome Analysis Reveals the Molecular Mechanism of Resting Cyst Formation in Colpoda aspera.

Jiang, Chuanqi / Wei, Wei / Yan, Guanxiong / Shi, Tuanyuan / Miao, Wei

The Journal of eukaryotic microbiology

2018 Volume 66, Issue 2, Page(s) 212–220

Abstract: Resting cyst formation is a remarkable survival strategy used by ciliates in response to the adverse environmental conditions. However, the mechanisms underlying encystment are poorly understood. Here, the genetic basis of encystment in Colpoda aspera ... ...

Abstract	Resting cyst formation is a remarkable survival strategy used by ciliates in response to the adverse environmental conditions. However, the mechanisms underlying encystment are poorly understood. Here, the genetic basis of encystment in Colpoda aspera was examined through RNA sequencing to identify transcriptome-wide changes in gene expression between vegetative and encystment stages. After de novo assembly, 49,543 transcripts were identified. Gene annotation and pathway mapping analysis revealed marked changes in biosynthesis, energy metabolism, and autophagy pathways during cyst formation. In addition, some differentially regulated genes were predicted to function in the interconnected cAMP, AMPK, mTOR, and PI3K/AKT signaling pathways, potentially forming a regulatory network for encystment. The present study conducted a large-scale assessment of Colpoda aspera genomic resources and provides new insight into the molecular mechanisms underlying cyst formation.
MeSH term(s)	Ciliophora/genetics ; Ciliophora/physiology ; Gene Expression Profiling ; Genes, Protozoan ; Sequence Analysis, RNA ; Transcriptome
Language	English
Publishing date	2018-07-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1147218-2
ISSN	1550-7408 ; 1066-5234
ISSN (online)	1550-7408
ISSN	1066-5234
DOI	10.1111/jeu.12643
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Article: An Investigation of the Relationship between

Shi, Tuanyuan / Yan, Xinlei / Sun, Hongchao / Fu, Yuan / Hao, Lili / Zhou, Yongxue / Liu, Yan / Han, Wenying / Bao, Guolian / Suo, Xun

Pathogens (Basel, Switzerland)

2021 Volume 10, Issue 7

Abstract: Cyniclomyces ... ...

Abstract	Cyniclomyces guttulatus
Language	English
Publishing date	2021-07-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10070880
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Article: An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Shi, Tuanyuan / Yan, Xinlei / Sun, Hongchao / Fu, Yuan / Hao, Lili / Zhou, Yongxue / Liu, Yan / Han, Wenying / Bao, Guolian / Suo, Xun

Pathogens. 2021 July 12, v. 10, no. 7

2021

Abstract: Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit ... ...

Abstract	Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 10⁸ vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.
Keywords	New Zealand White rabbit ; body weight changes ; coccidiosis ; culture media ; diarrhea ; digestive tract ; feces ; food intake ; gastrointestinal system ; opportunistic pathogens ; pH ; rabbits ; ribosomal DNA ; temperature ; yeasts ; China
Language	English
Dates of publication	2021-0712
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10070880
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