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Book ; Online: Human Attention Detection Using AM-FM Representations

Shi, Wenjing

2022

Abstract: Human activity detection from digital videos presents many challenges to the computer vision and image processing communities. Recently, many methods have been developed to detect human activities with varying degree of success. Yet, the general human ... ...

Abstract	Human activity detection from digital videos presents many challenges to the computer vision and image processing communities. Recently, many methods have been developed to detect human activities with varying degree of success. Yet, the general human activity detection problem remains very challenging, especially when the methods need to work 'in the wild' (e.g., without having precise control over the imaging geometry). The thesis explores phase-based solutions for (i) detecting faces, (ii) back of the heads, (iii) joint detection of faces and back of the heads, and (iv) whether the head is looking to the left or the right, using standard video cameras without any control on the imaging geometry. The proposed phase-based approach is based on the development of simple and robust methods that rely on the use of Amplitude Modulation- Frequency Modulation (AM-FM) models. The approach is validated using video frames extracted from the Advancing Out-of-school Learning in Mathematics and Engineering (AOLME) project. The dataset consisted of 13,265 images from ten students looking at the camera, and 6,122 images from five students looking away from the camera. For the students facing the camera, the method was able to correctly classify 97.1% of them looking to the left and 95.9% of them looking to the right. For the students facing the back of the camera, the method was able to correctly classify 87.6% of them looking to the left and 93.3% of them looking to the right. The results indicate that AM-FM based methods hold great promise for analyzing human activity videos. Comment: Masters thesis
Keywords	Computer Science - Computer Vision and Pattern Recognition ; Electrical Engineering and Systems Science - Image and Video Processing
Subject code	020
Publishing date	2022-03-08
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Calcium, phosphorus, magnesium levels in frequent respiratory tract infections.

Mao, Song / Wu, Liangxia / Shi, Wenjing

Annals of medicine

2024 Volume 55, Issue 2, Page(s) 2304661

Abstract: Background: It was well documented that calcium (Ca), phosphorus (P), and magnesium (Mg) participate in many physiological processes. We aimed to study the changing trend of serum levels of Ca, P, and Mg in frequent respiratory tract infections (FRTI) ... ...

Abstract	Background: It was well documented that calcium (Ca), phosphorus (P), and magnesium (Mg) participate in many physiological processes. We aimed to study the changing trend of serum levels of Ca, P, and Mg in frequent respiratory tract infections (FRTI) in children. Methods: A retrospective study was performed in our centre. A total of 213 FRTI cases and 33 controls were enrolled in our study. We analyzed the correlation between serum Ca/P/Mg levels and inflammatory indexes by using Spearman correlation analysis. Standard mean difference (SMD) was applied to test the differences of serum Ca/P/Mg levels between FRTI subgroups and controls. In terms of the findings of SMD between Ca/P/Mg status between FRTI subgroups and controls, receiver operating characteristics (ROC) curve analysis was further applied to test the association between serum Ca level and bronchitis, parainfluenza virus infection, influenza B virus infection and cytomegalovirus infection. Results: Serum Ca level was significantly associated with white blood cell (WBC), platelet (PLT) and procalcitonin (PCT) ( Conclusions: Serum levels of Ca and P may reflect the inflammatory status in children with FRTI. Alteration of serum Ca level may predict the risk of bronchitis and influenza B virus infection. Keeping the homeostasis of Ca, P, and Mg may be important for the prevention and treatment of FRTI.
MeSH term(s)	Child ; Humans ; Influenza, Human/epidemiology ; Calcium ; Respiratory Tract Infections ; Magnesium ; Phosphorus ; Retrospective Studies ; Bronchitis ; Paramyxoviridae Infections ; Cytomegalovirus Infections ; Herpesviridae Infections
Chemical Substances	Calcium (SY7Q814VUP) ; Magnesium (I38ZP9992A) ; Phosphorus (27YLU75U4W)
Language	English
Publishing date	2024-01-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1004226-x
ISSN	1365-2060 ; 1651-2219 ; 0785-3890 ; 1743-1387
ISSN (online)	1365-2060 ; 1651-2219
ISSN	0785-3890 ; 1743-1387
DOI	10.1080/07853890.2024.2304661
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Article ; Online: Community-embedded follow-up management intervention for geriatric primary care: a mixed-methods study of an integrated health services model.

Shi, Wenjing / Wu, Lingling / Li, Xiaodong / Qi, Feng / Ji, Wanyu

BMC health services research

2024 Volume 24, Issue 1, Page(s) 298

Abstract: Background: To propose a community-embedded follow-up management model to provide health services for elderly patients with osteoporosis who live alone.: Methods: Researchers randomly selected 396 people with osteoporosis living alone from five ... ...

Abstract	Background: To propose a community-embedded follow-up management model to provide health services for elderly patients with osteoporosis who live alone. Methods: Researchers randomly selected 396 people with osteoporosis living alone from five communities in Nantong, China, for the study. These participants were randomly assigned to control and intervention groups. Twenty-four community physicians in five communities provided professional support based on a community-embedded follow-up management model. Participants completed quantitative questionnaires at baseline and after the 6-month follow-up intervention, and some participants underwent semi-structured face-to-face interviews. The primary outcome is the effectiveness of the community-embedded follow-up management model in improving the quality of life of elderly patients with osteoporosis living alone. Based on an objective quantitative assessment, the qualitative study explains and adds essential components of this community-based follow-up management model. Results: The quantitative study showed that scores in physical functioning, ability to perform daily activities, self-efficacy, and mental status were significantly improved in the intervention group compared to the control group (p < 0.05). The most significant improvements were found in "mental status" (p = 0.012) and "self-care skills" (p = 0.003). The qualitative study reported the essential elements of a community healthcare model for older people living alone with osteoporosis, including professional support, personalized services, social support, and empowerment. Conclusions: Community-embedded follow-up management meets the need for elderly patients with osteoporosis living alone. It helps to improve health perception, promote physical and mental health, and optimize the quality of life in this population. Personalized services and professional support are two major contributing factors to effective embedded follow-up management in the community.
MeSH term(s)	Aged ; Humans ; Follow-Up Studies ; Quality of Life ; Health Services ; Osteoporosis/therapy ; Primary Health Care
Language	English
Publishing date	2024-03-06
Publishing country	England
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	2050434-2
ISSN	1472-6963 ; 1472-6963
ISSN (online)	1472-6963
ISSN	1472-6963
DOI	10.1186/s12913-024-10804-8
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Article: Penehyclidine hydrochloride alleviates LPS-induced inflammatory responses and oxidative stress via ROS/Nrf2/HO-1 activation in RAW264.7 cells.

Guo, Qiongmei / Zhang, Chunyan / Gao, Jingui / Shi, Wenjing / Liu, Xiaozhi

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

2024

Abstract: Background: Inflammation is a biological response of the immune system to harmful stimuli. Penehyclidine hydrochloride (PCH) can alleviate inflammation and oxidative stress by activating reactive oxygen species (ROS), nuclear factor erythroid 2-related ... ...

Abstract	Background: Inflammation is a biological response of the immune system to harmful stimuli. Penehyclidine hydrochloride (PCH) can alleviate inflammation and oxidative stress by activating reactive oxygen species (ROS), nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase 1 (HO-1) in animal models, but there is a lack of cellular evidence. Objectives: This study investigated the effects of PHC on lipopolysaccharide (LPS)-induced inflammation response and oxidative stress in RAW264.7 cells. Material and methods: RAW264.7 cells were treated with 1 μg/mL or 5 μg/mL of PHC, with interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), IL-1β, and prostaglandin E2 (PGE2) levels measured with enzyme-linked immunosorbent assay (ELISA) and nitric oxide (NO) measured using the Griess test. Reactive oxygen species were examined with flow cytometry and immunofluorescence, and b-related factor 2 (BRF-2) and NAD(P)H-quinone oxidoreductase 1 (NQO1) using western blot. Results: Penehyclidine hydrochloride partly, but substantially, reversed LPS-related NO and PGE2 production by RAW264.7 cells in a dose-dependent manner and suppressed LPS-induced expression of IL-6, TNF-α and IL-1β messenger ribonucleic acid (mRNA), secretion of IL-6, TNF-α and IL-1β, and ROS production. Lipopolysaccharide stimulation did not affect Nrf2, heme oxygenase 1 (HO-1) or NQO1 protein expression in RWA264.7 cells not treated with PHC. However, PHC treatment significantly elevated Nrf2, HO-1 and NQO1 protein in LPS-treated RWA264.7 cells, an effect that was dose-dependent. The ROS scavenging using N-acetyl-L-cysteine abolished the PHC-induced upregulation of Nrf2 and HO-1. Conclusions: Penehyclidine hydrochloride may alleviate LPS-induced inflammation and oxidative stress by activating Nrf2 signaling in RAW264.7 macrophages. These findings suggest that PHC could alleviate inflammation by targeting activated macrophages.
Language	English
Publishing date	2024-03-26
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2270257-X
ISSN	1899-5276 ; 1230-025X
ISSN	1899-5276 ; 1230-025X
DOI	10.17219/acem/183883
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Article ; Online: Construction and evaluation of near-infrared fluorescent probes for imaging lipid droplet and lysosomal viscosity.

Li, Yuming / Wang, Yuxuan / Li, Yanping / Shi, Wenjing / Yan, Jinwu

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2024 Volume 316, Page(s) 124356

Abstract: Microenvironmental viscosity is a crucial parameter for biological systems, and its abnormal fluctuations are closely associated with various functional disorders and diseases. However, it is still important and urgent to develop improved near-infrared ... ...

Abstract	Microenvironmental viscosity is a crucial parameter for biological systems, and its abnormal fluctuations are closely associated with various functional disorders and diseases. However, it is still important and urgent to develop improved near-infrared fluorescent probes for micro-viscosity with dual-organelle targeting properties, low background noise, and high sensitivity. Herein, two BODIPY-based small-molecule fluorescent probes were designed and synthesized, which were explored for their viscosity- and polarity-responsive properties, and were further applied to imaging sub-cellular viscosity in living cells. Interestingly, BSZ-Ph and BSZ-R displayed near-infrared fluorescence (more than 650 nm) and were sensitive to environmental viscosity and polarity due to the introduction of a benzothiazole at the 2-position and electron-rich aniline groups at the 5-position of the BODIPY core, respectively. The fluorescence intensity increased exponentially with the viscosity changes. Furthermore, the probe BSZ-Ph could successfully target lipid droplets and image cellular viscosity changes by treating lipopolysaccharides (LPS) and nystatin. Comparatively, the probe BSZ-R could successfully target the dual organelles of lipid droplets and lysosomes and image cellular viscosity changes by treating LPS and monensin. Therefore, in this work, we reported two new BODIPY-based near-infrared fluorescent probes, BSZ-Ph and BSZ-R, for cellular viscosity imaging, which could target lipid droplets and the dual organelles of lysosomes and lipid droplets, respectively. The study could provide a reference for the future development of fluorescent probes for viscosity in lipid droplets and lysosomes.
MeSH term(s)	Fluorescent Dyes/chemistry ; Fluorescent Dyes/chemical synthesis ; Viscosity ; Lysosomes/metabolism ; Lysosomes/chemistry ; Lipid Droplets/chemistry ; Lipid Droplets/metabolism ; Boron Compounds/chemistry ; Boron Compounds/chemical synthesis ; Humans ; Animals ; Mice ; HeLa Cells ; Optical Imaging
Chemical Substances	Fluorescent Dyes ; Boron Compounds ; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Language	English
Publishing date	2024-04-26
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2024.124356
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Article: Changing trend of neonatal Ca/P/Mg status in a Chinese population.

Mao, Song / Wu, Liangxia / Shi, Wenjing

Translational pediatrics

2023 Volume 12, Issue 9, Page(s) 1659–1667

Abstract: Background: Calcium (Ca), phosphorus (P), and magnesium (Mg) are essential elements for keeping the body homeostasis. We aimed to investigate the changing trend of serum levels of Ca/P/Mg in neonates.: Methods: We enrolled 82 premature newborns, 173 ... ...

Abstract	Background: Calcium (Ca), phosphorus (P), and magnesium (Mg) are essential elements for keeping the body homeostasis. We aimed to investigate the changing trend of serum levels of Ca/P/Mg in neonates. Methods: We enrolled 82 premature newborns, 173 neonatal sepsis, 50 neonatal hypoglycemia, 254 neonatal jaundice, 43 neonatal haemolytic disease, and 59 healthy controls in our retrospective study. Serum levels of Ca/P/Mg were collected and expressed in quarters. We analysed the association between neonatal disorders and Ca/P/Mg levels (fourth quarter Results: Binary logistic regression analyses showed that neonatal haemolytic disease, hypoglycemia, sepsis, jaundice, and prematurity were all significantly associated with the fourth quarter of Ca level (P<10 Conclusions: Neonatal disorders demonstrated a decreasing trend of serum Ca/P level. A significantly non-linear association was observed between birthweight/PCT and serum Ca/P levels.
Language	English
Publishing date	2023-09-11
Publishing country	China
Document type	Journal Article
ZDB-ID	2901309-4
ISSN	2224-4344 ; 2224-4344 ; 2224-4336
ISSN (online)	2224-4344
ISSN	2224-4344 ; 2224-4336
DOI	10.21037/tp-23-129
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Article: c-MYC-Induced AP4 Attenuates DREAM-Mediated Repression by p53.

Kaller, Markus / Shi, Wenjing / Hermeking, Heiko

Cancers

2023 Volume 15, Issue 4

Abstract: Background: The deregulated expression of the c-MYC oncogene activates p53, which is presumably mediated by ARF/INK4, as well as replication-stress-induced DNA damage. Here, we aimed to determine whether the c-MYC-inducible AP4 transcription factor ... ...

Abstract	Background: The deregulated expression of the c-MYC oncogene activates p53, which is presumably mediated by ARF/INK4, as well as replication-stress-induced DNA damage. Here, we aimed to determine whether the c-MYC-inducible AP4 transcription factor plays a role in this context using a genetic approach. Methods: We used a CRISPR/Cas9 approach to generate Results: Loss of Conclusions: Our results establish AP4 as a pivotal factor at the crossroads of c-MYC, E2F, and p53 target gene regulation.
Language	English
Publishing date	2023-02-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15041162
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Article ; Online: Predictive and prognostic value of leptin status in asthma.

Wang, Juan / Zhu, Ruochen / Shi, Wenjing / Mao, Song

NPJ primary care respiratory medicine

2023 Volume 33, Issue 1, Page(s) 10

Abstract: Asthma is closely associated with inflammation. We evaluated the predictive and prognostic value of leptin status in asthma. We searched the electronic databases for articles that determined the leptin level in asthma cases through May 2020. We compared ... ...

Abstract	Asthma is closely associated with inflammation. We evaluated the predictive and prognostic value of leptin status in asthma. We searched the electronic databases for articles that determined the leptin level in asthma cases through May 2020. We compared the differences of leptin level between asthma and non-asthma controls, as well as between severe and mild asthma cases. We also investigated the impact of age and gender on these differences by using meta-regression analysis. 59 studies were included in our pooled analysis. Asthma cases demonstrated significantly higher leptin level than that in non-asthma controls among overall populations (SMD:1.061, 95% CI: 0.784-1.338, p < 10
MeSH term(s)	Female ; Humans ; Male ; Asian ; Asthma/diagnosis ; Leptin ; Prognosis ; White People ; Black People
Chemical Substances	Leptin
Language	English
Publishing date	2023-03-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2780812-9
ISSN	2055-1010 ; 2055-1010
ISSN (online)	2055-1010
ISSN	2055-1010
DOI	10.1038/s41533-023-00332-z
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Article: ZEB1 promotes DNA homologous recombination repair and contributes to the 5-Fluorouracil resistance in colorectal cancer.

Wu, Chao / Shi, Wenjing / Zhang, Sen

American journal of cancer research

2023 Volume 13, Issue 9, Page(s) 4101–4114

Abstract: Chemotherapy resistance represents a significant obstacle in clinical practice of colorectal cancer (CRC). In this study we aim to clarify the underlying mechanism of chemotherapy resistance mediated by ZEB1 in CRC. shRNA-mediated repression of ZEB1 ... ...

Abstract	Chemotherapy resistance represents a significant obstacle in clinical practice of colorectal cancer (CRC). In this study we aim to clarify the underlying mechanism of chemotherapy resistance mediated by ZEB1 in CRC. shRNA-mediated repression of ZEB1 induced DNA damage in SW480 and RKO cells. Ectopic expression of ZEB1 suppressed the DNA damage caused by
Language	English
Publishing date	2023-09-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2589522-9
ISSN	2156-6976
ISSN	2156-6976
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Article ; Online: Risk prediction for liver injury in Epstein-Barr virus infection in pediatric respiratory tract infections.

Mao, Song / Wu, Liangxia / Shi, Wenjing

Italian journal of pediatrics

2023 Volume 49, Issue 1, Page(s) 138

Abstract: Background: Epstein-Barr virus (EBV) infection is likely to co-occur in pediatric respiratory tract infections (RTIs). Liver injury is the common complication of EBV infection. The detailed risk factors for liver injury in EBV infection remain elusive. ... ...

Abstract	Background: Epstein-Barr virus (EBV) infection is likely to co-occur in pediatric respiratory tract infections (RTIs). Liver injury is the common complication of EBV infection. The detailed risk factors for liver injury in EBV infection remain elusive. We aimed to investigate the incidence, characteristics and potential risk factors for liver injury in EBV infection for early risk prediction. Methods: We retrospectively recruited the pediatric RTIs cases with EBV infection according to a predefined criteria from our hospital between January 2015 and December 2017. We extracted the clinical and laboratory data from the electronical medical records. The impact of age, gender, and various parameters on the liver injury risk was investigated. Univariate logistic regression analysis was performed to analyse the association between clinical/laboratory parameters and liver injury. The related indexes were enrolled in the multivariate logistic regression analysis. Decision curve analysis was used to yield the value of related parameters in predicting liver injury. Receiver operating curve (ROC) analysis was applied to produce the C-index of white blood cell (WBC) count for liver injury. We also tested the non-linear association between WBC count and alanine aminotransferase (ALT). Results: A total of 216 pediatric RTIs with EBV infection were enrolled. EBV infection is more likely to occur during the winter season. Cytomegalovirus infection was independently associated with liver injury in EBV infection (OR = 6.972, 95% CI = 1.648-29.490, p = 0.008). WBC count was independently associated with liver injury in EBV infection (OR = 1.169, 95% CI = 1.051-1.301, p = 0.004). The P interaction value between WBC count and cytomegalovirus was 0.149. The decision curve analysis showed that WBC count had larger area under curve compared with platelet (PLT) and birthweight (BW). ROC analysis yielded the c-index of WBC count: 0.75 and cut-point of 8.3. The turning point of WBC count in its association with ALT was 16.8. The p value before and after the turning point was < 0.001 and 0.123, respectively. Conclusions: Cytomegalovirus co-infection demonstrated 5.972 more times of liver injury risk in EBV infection. WBC count was an independent biomarker for liver injury before the turning point of 16.8 in EBV infection. More attention should be paid to the risk of EBV infection in the winter. Cytomegalovirus infection and WBC count merit attention in the monitoring of possible liver injury in EBV infection among pediatric RTIs.
MeSH term(s)	Humans ; Child ; Epstein-Barr Virus Infections/complications ; Epstein-Barr Virus Infections/diagnosis ; Epstein-Barr Virus Infections/epidemiology ; Herpesvirus 4, Human ; Retrospective Studies ; Liver ; Cytomegalovirus Infections/complications ; Cytomegalovirus Infections/diagnosis ; Cytomegalovirus Infections/epidemiology ; Cytomegalovirus ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/epidemiology
Language	English
Publishing date	2023-10-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2088556-8
ISSN	1824-7288 ; 1720-8424
ISSN (online)	1824-7288
ISSN	1720-8424
DOI	10.1186/s13052-023-01546-0
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