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Article ; Online: Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H

Fuji, Saashia / Yamauchi, Shota / Sugiyama, Naoyuki / Kohchi, Takayuki / Nishihama, Ryuichi / Shimazaki, Ken-Ichiro / Takemiya, Atsushi

Nature communications

2024 Volume 15, Issue 1, Page(s) 1195

Abstract: Plasma membrane ... ...

Abstract	Plasma membrane H
MeSH term(s)	Phosphorylation ; Light ; Plant Stomata/metabolism ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Proton-Translocating ATPases/genetics ; Proton-Translocating ATPases/metabolism ; Cell Membrane/metabolism
Chemical Substances	Arabidopsis Proteins ; Proton-Translocating ATPases (EC 3.6.3.14)
Language	English
Publishing date	2024-02-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45236-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Determination of H

Yamauchi, Shota / Shimazaki, Ken-Ichiro

Bio-protocol

2017 Volume 7, Issue 24, Page(s) e2653

Abstract: The opening of stomata in plants in response to blue light is driven by the plasma membrane ... ...

Abstract	The opening of stomata in plants in response to blue light is driven by the plasma membrane H
Language	English
Publishing date	2017-12-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2833269-6
ISSN	2331-8325 ; 2331-8325
ISSN (online)	2331-8325
ISSN	2331-8325
DOI	10.21769/BioProtoc.2653
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Article ; Online: Arabidopsis phot1 and phot2 phosphorylate BLUS1 kinase with different efficiencies in stomatal opening.

Takemiya, Atsushi / Shimazaki, Ken-ichiro

Journal of plant research

2016 Volume 129, Issue 2, Page(s) 167–174

Abstract: In Arabidopsis thaliana, phototropins (phot1 and phot2), light-activated receptor kinases, redundantly regulate various photoresponses such as phototropism, chloroplast photorelocation movement, stomatal opening, and leaf flattening. However, it is still ...

Abstract	In Arabidopsis thaliana, phototropins (phot1 and phot2), light-activated receptor kinases, redundantly regulate various photoresponses such as phototropism, chloroplast photorelocation movement, stomatal opening, and leaf flattening. However, it is still unclear how phot1 and phot2 signals are integrated into a common target and regulate physiological responses. In the present study, we provide evidence that phot1 and phot2 phosphorylate BLUE LIGHT SIGNALING1 (BLUS1) kinase as a common substrate in stomatal opening. Biochemical analysis revealed that the recombinant phot2 protein directly phosphorylated BLUS1 in vitro in a blue light-dependent manner, as reported for phot1. BLUS1 phosphorylation was observed in both phot1 and phot2 mutants, and phot2 mutant exhibited higher phosphorylation of BLUS1 than did phot1 mutant. Transgenic plants expressing phot1-GFP (P1G) and phot2-GFP (P2G) at a similar level under the PHOT2 promoter demonstrated that P1G initiated higher phosphorylation of BLUS1 than P2G, suggesting that phot1 phosphorylates BLUS1 more efficiently. Similarly, P1G mediated a higher activation of the plasma membrane H(+)-ATPase and stomatal opening than P2G, indicating that the phosphorylation status of BLUS1 is a key determinant of physiological response. Together, these findings provide insights into the signal integration and different properties of phot1 and phot2 signaling.
MeSH term(s)	Arabidopsis/enzymology ; Arabidopsis/genetics ; Arabidopsis/physiology ; Arabidopsis/radiation effects ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Genes, Reporter ; Light ; Light Signal Transduction ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; Phosphorylation ; Phosphotransferases ; Phototropins/genetics ; Phototropins/metabolism ; Phototropism ; Plant Leaves/enzymology ; Plant Leaves/genetics ; Plant Leaves/physiology ; Plant Leaves/radiation effects ; Plant Stomata/enzymology ; Plant Stomata/genetics ; Plant Stomata/physiology ; Plant Stomata/radiation effects ; Plants, Genetically Modified ; Proton-Translocating ATPases/genetics ; Proton-Translocating ATPases/metabolism ; Recombinant Fusion Proteins
Chemical Substances	Arabidopsis Proteins ; BLUS1 protein, Arabidopsis ; NPH1 protein, Arabidopsis ; PHOT2 protein, Arabidopsis ; Phosphoproteins ; Phototropins ; Recombinant Fusion Proteins ; Phosphotransferases (EC 2.7.-) ; Proton-Translocating ATPases (EC 3.6.3.14)
Language	English
Publishing date	2016-03
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2077362-6
ISSN	1618-0860 ; 0918-9440
ISSN (online)	1618-0860
ISSN	0918-9440
DOI	10.1007/s10265-015-0780-1
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Article ; Online: Reconstitution of Abscisic Acid Signaling from the Receptor to DNA via bHLH Transcription Factors.

Takahashi, Yohei / Ebisu, Yuta / Shimazaki, Ken-Ichiro

Plant physiology

2017 Volume 174, Issue 2, Page(s) 815–822

Abstract: The plant hormone abscisic acid (ABA) confers drought tolerance in plants through stomatal closure and regulation of gene expression. The complex consisting of the ABA receptor PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR), type ... ...

Abstract	The plant hormone abscisic acid (ABA) confers drought tolerance in plants through stomatal closure and regulation of gene expression. The complex consisting of the ABA receptor PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR), type 2C protein phosphatase (PP2C), and SNF1-related protein kinase 2 (SnRK2) has a key role in ABA signaling. Basic helix-loop-helix (bHLH) transcriptional activator ABA-RESPONSIVE KINASE SUBSTRATE1 (AKS1, also known as FBH3) is released from DNA by phosphorylation-induced monomerization in response to ABA in guard cells. Here we reconstituted the release of AKS1 from DNA via the ABA signaling core complex in vitro. We first obtained evidence to confirm that AKS1 is an endogenous substrate for SnRK2s. Phosphorylation of AKS1 and activation of SnRK2 showed the same time course in response to ABA in guard cells. AKS1 was bound to SnRK2.6 in vivo. Three ABA-responsive SnRK2s (SnRK2.2/SRK2D, SnRK2.3/SRK2I, and SnRK2.6/SRK2E/OST1) phosphorylated AKS1 in vitro, and the phosphorylation was eliminated by the triple mutation of SnRK2s in plants. We reconstituted the AKS1 phosphorylation in vitro via the signaling complex containing the ABA receptor PYR1, a PP2C, HYPERSENSITIVE TO ABA1 (HAB1), and a protein kinase, SnRK2.6 in response to ABA We further reconstituted the release of AKS1 from the target gene of
Language	English
Publishing date	2017-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	208914-2
ISSN	1532-2548 ; 0032-0889
ISSN (online)	1532-2548
ISSN	0032-0889
DOI	10.1104/pp.16.01825
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Article: Guard cell photosynthesis is crucial in abscisic acid‐induced stomatal closure

Iwai, Sumio / Ogata, Sho / Yamada, Naotaka / Onjo, Michio / Sonoike, Kintake / Shimazaki, Ken‐ichiro

Plant direct. 2019 May, v. 3, no. 5

2019

Abstract: Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in ... ...

Abstract	Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA‐elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3‐(3, 4‐dichlorophenyl)‐1, 1‐dimethylurea and 2, 5‐dibromo‐3‐methyl‐6‐isopropyl‐p‐benzoquinone. These inhibitors reduced ABA‐induced stomatal closure in all three species, as well as in the NADPH oxidase‐lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA‐induced ROS in the chloroplasts, and ABA‐induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA‐induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.
Keywords	Arabidopsis thaliana ; Commelina benghalensis ; NAD(P)H oxidase (H2O2-forming) ; Vicia faba ; abscisic acid ; chloroplasts ; guard cells ; mutants ; photosynthetic electron transport ; reactive oxygen species ; stomatal movement
Language	English
Dates of publication	2019-05
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ISSN	2475-4455
DOI	10.1002/pld3.137
Database	NAL-Catalogue (AGRICOLA)

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Article: Stomatal response to blue light in crassulacean acid metabolism plants Kalanchoe pinnata and Kalanchoe daigremontiana

Gotoh, Eiji / Oiwamoto, Kohei / Inoue, Shin-ichiro / Shimazaki, Ken-ichiro / Doi, Michio

Journal of experimental botany. 2019 Feb. 20, v. 70, no. 4

2019

Abstract: Blue light (BL) is a fundamental cue for stomatal opening in both C3 and C4 plants. However, it is unknown whether crassulacean acid metabolism (CAM) plants open their stomata in response to BL. We investigated stomatal BL responses in the obligate CAM ... ...

Abstract	Blue light (BL) is a fundamental cue for stomatal opening in both C3 and C4 plants. However, it is unknown whether crassulacean acid metabolism (CAM) plants open their stomata in response to BL. We investigated stomatal BL responses in the obligate CAM plants Kalanchoe pinnata and Kalanchoe daigremontiana that characteristically open their stomata at night and close them for part of the day, as contrasted with C3 and C4 plants. Stomata opened in response to weak BL superimposed on background red light in both intact leaves and detached epidermal peels of K. pinnata and K. daigremontiana. BL-dependent stomatal opening was completely inhibited by tautomycin and vanadate, which repress type 1 protein phosphatase and plasma membrane H+-ATPase, respectively. The plasma membrane H+-ATPase activator fusicoccin induced stomatal opening in the dark. Both BL and fusicoccin induced phosphorylation of the guard cell plasma membrane H+-ATPase in K. pinnata. These results indicate that BL-dependent stomatal opening occurs in the obligate CAM plants K. pinnata and K. daigremontiana independently of photosynthetic CO2 assimilation mode.
Keywords	C3 plants ; C4 plants ; Crassulacean acid metabolism ; H-transporting ATP synthase ; Kalanchoe daigremontiana ; Kalanchoe pinnata ; blue light ; carbon dioxide ; fusicoccin ; guard cells ; phosphorylation ; plasma membrane ; red light ; stomata ; stomatal movement
Language	English
Dates of publication	2019-0220
Size	p. 1367-1374.
Publishing place	Oxford University Press
Document type	Article
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/ery450
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diverse responses to blue light via LOV photoreceptors.

Shimazaki, Ken-ichiro / Tokutomi, Satoru

Plant & cell physiology

2013 Volume 54, Issue 1, Page(s) 1–4

MeSH term(s)	Chlorophyta/physiology ; Light ; Photoreceptors, Plant/chemistry ; Photoreceptors, Plant/physiology ; Phototropins/physiology ; Protein Structure, Tertiary ; Signal Transduction
Chemical Substances	Photoreceptors, Plant ; Phototropins
Language	English
Publishing date	2013-01
Publishing country	Japan
Document type	Editorial ; Research Support, Non-U.S. Gov't
ZDB-ID	208907-5
ISSN	1471-9053 ; 0032-0781
ISSN (online)	1471-9053
ISSN	0032-0781
DOI	10.1093/pcp/pcs172
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Article ; Online: A BLUS1 kinase signal and a decrease in intercellular CO2 concentration are necessary for stomatal opening in response to blue light.

Hosotani, Sakurako / Yamauchi, Shota / Kobayashi, Haruki / Fuji, Saashia / Koya, Shigekazu / Shimazaki, Ken-Ichiro / Takemiya, Atsushi

The Plant cell

2021 Volume 33, Issue 5, Page(s) 1813–1827

Abstract: Light-induced stomatal opening stimulates CO2 uptake and transpiration in plants. Weak blue light under strong red light effectively induces stomatal opening. Blue light-dependent stomatal opening initiates light perception by phototropins, and the ... ...

Abstract	Light-induced stomatal opening stimulates CO2 uptake and transpiration in plants. Weak blue light under strong red light effectively induces stomatal opening. Blue light-dependent stomatal opening initiates light perception by phototropins, and the signal is transmitted to a plasma membrane H+-ATPase in guard cells via BLUE LIGHT SIGNALING 1 (BLUS1) kinase. However, it is unclear how BLUS1 transmits the signal to H+-ATPase. Here, we characterized BLUS1 signaling in Arabidopsis thaliana, and showed that the BLUS1 C-terminus acts as an auto-inhibitory domain and that phototropin-mediated Ser-348 phosphorylation within the domain removes auto-inhibition. C-Terminal truncation and phospho-mimic Ser-348 mutation caused H+-ATPase activation in the dark, but did not elicit stomatal opening. Unexpectedly, the plants exhibited stomatal opening under strong red light and stomatal closure under weak blue light. A decrease in intercellular CO2 concentration via red light-driven photosynthesis together with H+-ATPase activation caused stomatal opening. Furthermore, phototropins caused H+-ATPase dephosphorylation in guard cells expressing constitutive signaling variants of BLUS1 in response to blue light, possibly for fine-tuning stomatal opening. Overall, our findings provide mechanistic insights into the blue light regulation of stomatal opening.
MeSH term(s)	Arabidopsis/drug effects ; Arabidopsis/physiology ; Arabidopsis/radiation effects ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/metabolism ; Carbon Dioxide/pharmacology ; Light ; Models, Biological ; Mutation/genetics ; Phosphorylation/drug effects ; Phosphorylation/radiation effects ; Phosphoserine/metabolism ; Phosphotransferases/chemistry ; Phosphotransferases/metabolism ; Phototropins/metabolism ; Plant Stomata/drug effects ; Plant Stomata/physiology ; Plant Stomata/radiation effects ; Plants, Genetically Modified ; Protein Domains ; Proton-Translocating ATPases/metabolism
Chemical Substances	Arabidopsis Proteins ; BLUS1 protein, Arabidopsis ; Phototropins ; Carbon Dioxide (142M471B3J) ; Phosphoserine (17885-08-4) ; Phosphotransferases (EC 2.7.-) ; Proton-Translocating ATPases (EC 3.6.3.14)
Language	English
Publishing date	2021-03-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	623171-8
ISSN	1532-298X ; 1040-4651
ISSN (online)	1532-298X
ISSN	1040-4651
DOI	10.1093/plcell/koab067
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Article ; Online: Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure.

Iwai, Sumio / Ogata, Sho / Yamada, Naotaka / Onjo, Michio / Sonoike, Kintake / Shimazaki, Ken-Ichiro

Plant direct

2019 Volume 3, Issue 5, Page(s) e00137

Abstract	Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in
Language	English
Publishing date	2019-05-30
Publishing country	England
Document type	Journal Article
ISSN	2475-4455
ISSN (online)	2475-4455
DOI	10.1002/pld3.137
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Article ; Online: Stomatal Blue Light Response Is Present in Early Vascular Plants.

Doi, Michio / Kitagawa, Yuki / Shimazaki, Ken-ichiro

Plant physiology

2015 Volume 169, Issue 2, Page(s) 1205–1213

Abstract: Light is a major environmental factor required for stomatal opening. Blue light (BL) induces stomatal opening in higher plants as a signal under the photosynthetic active radiation. The stomatal BL response is not present in the fern species of ... ...

Abstract	Light is a major environmental factor required for stomatal opening. Blue light (BL) induces stomatal opening in higher plants as a signal under the photosynthetic active radiation. The stomatal BL response is not present in the fern species of Polypodiopsida. The acquisition of a stomatal BL response might provide competitive advantages in both the uptake of CO2 and prevention of water loss with the ability to rapidly open and close stomata. We surveyed the stomatal opening in response to strong red light (RL) and weak BL under the RL with gas exchange technique in a diverse selection of plant species from euphyllophytes, including spermatophytes and monilophytes, to lycophytes. We showed the presence of RL-induced stomatal opening in most of these species and found that the BL responses operated in all euphyllophytes except Polypodiopsida. We also confirmed that the stomatal opening in lycophytes, the early vascular plants, is driven by plasma membrane proton-translocating adenosine triphosphatase and K(+) accumulation in guard cells, which is the same mechanism operating in stomata of angiosperms. These results suggest that the early vascular plants respond to both RL and BL and actively regulate stomatal aperture. We also found three plant species that absolutely require BL for both stomatal opening and photosynthetic CO2 fixation, including a gymnosperm, C. revoluta, and the ferns Equisetum hyemale and Psilotum nudum.
MeSH term(s)	Arabidopsis/physiology ; Carbon Dioxide/metabolism ; Cycadopsida/physiology ; Ferns/physiology ; Light ; Magnoliopsida/physiology ; Plant Stomata/physiology ; Potassium/metabolism ; Proton-Translocating ATPases/metabolism
Chemical Substances	Carbon Dioxide (142M471B3J) ; Proton-Translocating ATPases (EC 3.6.3.14) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2015-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208914-2
ISSN	1532-2548 ; 0032-0889
ISSN (online)	1532-2548
ISSN	0032-0889
DOI	10.1104/pp.15.00134
Database	MEDical Literature Analysis and Retrieval System OnLINE

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