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  1. Article ; Online: Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea pallasii.

    Oda, Shoji / Igarashi, Yoshihiko / Ohtake, Hideki / Sakai, Kosuke / Shimizu, Nobuyoshi / Morisawa, Masaaki

    Development, growth & differentiation

    2023  Volume 37, Issue 3, Page(s) 257–261

    Abstract: Ripe unfertilized eggs of the Pacific herring, Clupea pallasii, release sperm-activating proteins into seawater at the time of fertilization. Five species of herring sperm-activating proteins (HSAP) with different pl values (4.8, 4.9, 5.0, 5.1 and 5.4) ... ...

    Abstract Ripe unfertilized eggs of the Pacific herring, Clupea pallasii, release sperm-activating proteins into seawater at the time of fertilization. Five species of herring sperm-activating proteins (HSAP) with different pl values (4.8, 4.9, 5.0, 5.1 and 5.4) were purified from the egg-conditioned medium by gel filtration and isoelectric focusing. Molecular mass of the HSAP (pl = 5.1), the major species of the five HSAP, was determined to be 8.1 kDa by mass spectrometry. Molecular weights of all of the HSAP were estimated to be 7700 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The first 20 amino acid sequences from N-terminal ends of three HSAP (pl = 4.9, 5.0 and 5.1) were almost identical, suggesting that the HSAP have similar structures.
    Language English
    Publishing date 2023-06-06
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 280433-5
    ISSN 1440-169X ; 0012-1592
    ISSN (online) 1440-169X
    ISSN 0012-1592
    DOI 10.1046/j.1440-169X.1995.t01-2-00003.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Aire gene is a central player in the autoimmune disease].

    Yamaguchi, Yoshitaka / Shimizu, Nobuyoshi

    Seikagaku. The Journal of Japanese Biochemical Society

    2015  Volume 87, Issue 3, Page(s) 362–372

    MeSH term(s) Animals ; Autoimmune Diseases/immunology ; Autoimmunity/genetics ; Autoimmunity/immunology ; Humans ; Polyendocrinopathies, Autoimmune/immunology ; Transcription Factors/immunology ; Transcription Factors/metabolism ; Transcriptional Activation/immunology ; AIRE Protein
    Chemical Substances Transcription Factors
    Language Japanese
    Publishing date 2015-11-24
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 282319-6
    ISSN 0037-1017
    ISSN 0037-1017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book: Aquatic genomics

    Shimizu, Nobuyoshi

    steps toward a great future

    2003  

    Author's details N. Shimizu ... [et al.], (eds.)
    MeSH term(s) Fishes/genetics ; Crustacea/genetics ; Genome ; Genomics/trends
    Language English
    Size xvi, 432 p. :, ill. (some col.) ;, 24 cm.
    Publisher Springer
    Publishing place Tokyo ; New York
    Document type Book
    ISBN 9784431703310 ; 4431703314
    Database Catalogue of the US National Library of Medicine (NLM)

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  4. Book: Aquatic genomics

    Shimizu, Nobuyoshi

    steps toward a great future

    2003  

    Author's details N. Shimizu ... (eds.)
    Keywords Fishes/Genetics ; Fishes/Genome mapping ; Shellfish/Genetics ; Shellfish/Genome mapping ; Genetik ; Wassertiere ; Fische
    Language English
    Size XVI, 432 S, Ill., graph. Darst., 24 cm
    Publisher Springer
    Publishing place Tokyo u.a.
    Document type Book
    Note Includes bibliographical references
    ISBN 4431703314 ; 9784431703310
    Database Former special subject collection: coastal and deep sea fishing

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  5. Article: [Drug discovery based on the human genome sciences].

    Shimizu, Nobuyoshi

    Gan to kagaku ryoho. Cancer & chemotherapy

    2002  Volume 29, Issue 1, Page(s) 1–6

    MeSH term(s) Base Sequence ; Chromosome Mapping/trends ; DNA/genetics ; Drugs, Essential/standards ; Human Genome Project ; Humans
    Chemical Substances Drugs, Essential ; DNA (9007-49-2)
    Language Japanese
    Publishing date 2002-01
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 604842-0
    ISSN 0385-0684
    ISSN 0385-0684
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Progress in the field of pulmonary medicine in the last 100 years: Lung transplantation].

    Shimizu, Nobuyoshi

    Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine

    2002  Volume 91, Issue 6, Page(s) 1695–1697

    MeSH term(s) Brain Death ; History, 20th Century ; Humans ; Living Donors ; Lung Diseases/history ; Lung Diseases/surgery ; Lung Transplantation/adverse effects ; Lung Transplantation/history ; Lung Transplantation/methods ; Patient Selection ; Tissue Donors ; Tissue and Organ Harvesting ; Transplantation
    Language Japanese
    Publishing date 2002-06-10
    Publishing country Japan
    Document type Historical Article ; Journal Article ; Review
    ZDB-ID 952816-7
    ISSN 1883-2083 ; 0021-5384
    ISSN (online) 1883-2083
    ISSN 0021-5384
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Dual promoter expression system with insulator ensures a stringent tissue-specific regulation of two reporter genes in the transgenic fish

    Shimizu, Atsushi / Shimizu, Nobuyoshi

    Transgenic research. 2013 Apr., v. 22, no. 2

    2013  

    Abstract: The precise control of spatiotemporal expression of target genes is crucial when establishing transgenic animals, and the introduction of genes for fluorescent marker proteins is inevitable for accelerating research at molecular levels. To assist this, ... ...

    Abstract The precise control of spatiotemporal expression of target genes is crucial when establishing transgenic animals, and the introduction of genes for fluorescent marker proteins is inevitable for accelerating research at molecular levels. To assist this, we constructed a novel dual promoter expression vector for two independent reporter genes, green fluorescent protein (GFP) and red fluorescent protein (mCherry). Their expression is designed under the control of two distinct tissue-specific promoters, e.g. zebrafish cardiac muscle-specific promoter (cmlc2) and medaka skeletal muscle-specific promoter (myl2) derived from the myosin light chain 2 genes, and they are placed in a head-to-head orientation. After microinjecting the dual promoter expression vector into fertilized eggs of medaka, the developing fish embryos and the resulting transgenic fish lines showed strong GFP signal in the whole body (skeletal muscle) and mCherry signal in the heart (cardiac muscle). However, weak GFP signal was observed in the heart, indicating a leakiness of the skeletal muscle promoter. To improve the stringency of dual promoter expression, we inserted two chicken-derived insulators, e.g. tandem copies of the core sequence (250 bp) of cHS4 (5′-hypersensitive site-4 chicken beta-globin insulator), in the boundary of two promoters. The dual promoter expression vector with insulator now ensured the stringent tissue-specific expression in the transgenic fish lines. Thus, our dual promoter expression system with insulator is compatible to the conventional IRES and fused reporter gene systems and will be an alternative method to produce the transgenic fishes.
    Keywords chickens ; eggs ; fish ; gene expression ; green fluorescent protein ; myocardium ; myosin light chains ; reporter genes ; skeletal muscle ; transgenic animals
    Language English
    Dates of publication 2013-04
    Size p. 435-444.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 31620-9
    ISSN 1573-9368 ; 0962-8819
    ISSN (online) 1573-9368
    ISSN 0962-8819
    DOI 10.1007/s11248-012-9653-8
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Dual promoter expression system with insulator ensures a stringent tissue-specific regulation of two reporter genes in the transgenic fish.

    Shimizu, Atsushi / Shimizu, Nobuyoshi

    Transgenic research

    2012  Volume 22, Issue 2, Page(s) 435–444

    Abstract: The precise control of spatiotemporal expression of target genes is crucial when establishing transgenic animals, and the introduction of genes for fluorescent marker proteins is inevitable for accelerating research at molecular levels. To assist this, ... ...

    Abstract The precise control of spatiotemporal expression of target genes is crucial when establishing transgenic animals, and the introduction of genes for fluorescent marker proteins is inevitable for accelerating research at molecular levels. To assist this, we constructed a novel dual promoter expression vector for two independent reporter genes, green fluorescent protein (GFP) and red fluorescent protein (mCherry). Their expression is designed under the control of two distinct tissue-specific promoters, e.g. zebrafish cardiac muscle-specific promoter (cmlc2) and medaka skeletal muscle-specific promoter (myl2) derived from the myosin light chain 2 genes, and they are placed in a head-to-head orientation. After microinjecting the dual promoter expression vector into fertilized eggs of medaka, the developing fish embryos and the resulting transgenic fish lines showed strong GFP signal in the whole body (skeletal muscle) and mCherry signal in the heart (cardiac muscle). However, weak GFP signal was observed in the heart, indicating a leakiness of the skeletal muscle promoter. To improve the stringency of dual promoter expression, we inserted two chicken-derived insulators, e.g. tandem copies of the core sequence (250 bp) of cHS4 (5'-hypersensitive site-4 chicken beta-globin insulator), in the boundary of two promoters. The dual promoter expression vector with insulator now ensured the stringent tissue-specific expression in the transgenic fish lines. Thus, our dual promoter expression system with insulator is compatible to the conventional IRES and fused reporter gene systems and will be an alternative method to produce the transgenic fishes.
    MeSH term(s) Animals ; Animals, Genetically Modified/growth & development ; Chickens/genetics ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins/genetics ; Insulator Elements/genetics ; Luminescent Proteins/genetics ; Organ Specificity/genetics ; Oryzias/genetics ; Promoter Regions, Genetic ; Zebrafish/genetics ; Zebrafish/growth & development ; Red Fluorescent Protein
    Chemical Substances Luminescent Proteins ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2012-09-16
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 31620-9
    ISSN 1573-9368 ; 0962-8819
    ISSN (online) 1573-9368
    ISSN 0962-8819
    DOI 10.1007/s11248-012-9653-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: CancerProView: a graphical image database of cancer-related genes and proteins.

    Mitsuyama, Susumu / Shimizu, Nobuyoshi

    Genomics

    2012  Volume 100, Issue 2, Page(s) 81–92

    Abstract: We have developed a graphical image database CancerProView (URL: http://cancerproview.dmb.med.keio.ac.jp/php/cpv.html) to assist the search for alterations of the motifs/domains in the cancer-related proteins that are caused by mutations in the ... ...

    Abstract We have developed a graphical image database CancerProView (URL: http://cancerproview.dmb.med.keio.ac.jp/php/cpv.html) to assist the search for alterations of the motifs/domains in the cancer-related proteins that are caused by mutations in the corresponding genes. For the CancerProView, we have collected various kinds of data on 180 cancer-related proteins in terms of the motifs/domains, genomic structures of corresponding genes, and 109 charts of the protein interaction pathways. Moreover, we have collected the relevant data on 1041 reference genes including 197 non-cancer disease-associated genes, and the nucleotide sequences for 2011 full-length cDNA's and the alternatively spliced transcript variants. Thus, the CancerProView database system would provide valuable information to facilitate basic cancer research as well as for designing new molecular diagnosis and drug discovery for cancers. The CancerProView database can be operated via Internet with any Web browser, and the system is freely available to interested users without ID and password.
    MeSH term(s) Databases, Genetic ; Genes, Neoplasm ; Genetic Predisposition to Disease ; Humans ; Internet ; Mutation ; Neoplasm Proteins/genetics ; Neoplasms/diagnosis ; Neoplasms/genetics ; User-Computer Interface
    Chemical Substances Neoplasm Proteins
    Language English
    Publishing date 2012-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 356334-0
    ISSN 1089-8646 ; 0888-7543
    ISSN (online) 1089-8646
    ISSN 0888-7543
    DOI 10.1016/j.ygeno.2012.05.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book: Aquatic genomics

    Shimizu, Nobuyoshi

    step toward a great future

    2002  

    Author's details N. Shimizu ... [et al.] (eds.)
    Keywords Fishes/Genome mapping. ; Shellfish/Genome mapping. ; Fishes/Genetics. ; Shellfish/Genetics.
    Language English
    Size xvi, 432 p. :, 120 fig. (12 col.) ;, 24 cm.
    Publisher Springer
    Publishing place New York
    Document type Book
    ISBN 4431703314 ; 9784431703310
    Database NAL-Catalogue (AGRICOLA)

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