LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Epigenetics in cardiovascular disease.

    Shirodkar, Apurva V / Marsden, Philip A

    Current opinion in cardiology

    2011  Volume 26, Issue 3, Page(s) 209–215

    Abstract: Purpose of review: To provide an overview of the biological processes implicated in chromatin-based pathways that control endothelial gene expression patterns in both health and disease and highlight how these processes are relevant to cardiovascular ... ...

    Abstract Purpose of review: To provide an overview of the biological processes implicated in chromatin-based pathways that control endothelial gene expression patterns in both health and disease and highlight how these processes are relevant to cardiovascular disease.
    Recent findings: Epigenetics refers to chromatin-based pathways important in the regulation of gene expression and includes three distinct, but highly interrelated, mechanisms: DNA methylation, histone density and posttranslational modifications, and RNA-based mechanisms. It is of great interest that epigenetic regulation of genes enriched in the vascular endothelium is a prominent regulatory pathway. How environmental cues within the vasculature, such as hemodynamic forces or hypoxia, influence these epigenetic mechanisms will be reviewed.
    Summary: Although a newer area for study, exciting new evidence identifies that epigenetic processes are highly dynamic and respond to a myriad of environmental stimuli. Integrating chromatin-based pathways into our understanding of gene expression offers newer insight into disease processes.
    MeSH term(s) Cardiovascular Diseases/genetics ; Chromatin ; DNA Methylation ; Endothelium, Vascular ; Epigenesis, Genetic ; Epigenomics ; Gene Expression ; Gene Regulatory Networks/genetics ; Hemodynamics ; Histones/metabolism ; Humans ; Hypertension/complications ; Shear Strength
    Chemical Substances Chromatin ; Histones
    Language English
    Publishing date 2011-02-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 645186-x
    ISSN 1531-7080 ; 0268-4705
    ISSN (online) 1531-7080
    ISSN 0268-4705
    DOI 10.1097/HCO.0b013e328345986e
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2556: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: A mechanistic role for DNA methylation in endothelial cell (EC)-enriched gene expression: relationship with DNA replication timing.

    Shirodkar, Apurva V / St Bernard, Rosanne / Gavryushova, Anna / Kop, Anna / Knight, Britta J / Yan, Matthew Shu-Ching / Man, Hon-Sum Jeffrey / Sud, Maneesh / Hebbel, Robert P / Oettgen, Peter / Aird, William C / Marsden, Philip A

    Blood

    2013  Volume 121, Issue 17, Page(s) 3531–3540

    Abstract: Proximal promoter DNA methylation has been shown to be important for regulating gene expression. However, its relative contribution to the cell-specific expression of endothelial cell (EC)-enriched genes has not been defined. We used methyl-DNA ... ...

    Abstract Proximal promoter DNA methylation has been shown to be important for regulating gene expression. However, its relative contribution to the cell-specific expression of endothelial cell (EC)-enriched genes has not been defined. We used methyl-DNA immunoprecipitation and bisulfite conversion to analyze the DNA methylation profile of EC-enriched genes in ECs vs nonexpressing cell types, both in vitro and in vivo. We show that prototypic EC-enriched genes exhibit functional differential patterns of DNA methylation in proximal promoter regions of most (eg, CD31, von Willebrand factor [vWF], VE-cadherin, and intercellular adhesion molecule-2), but not all (eg, VEGFR-1 and VEGFR-2), EC-enriched genes. Comparable findings were evident in cultured ECs, human blood origin ECs, and murine aortic ECs. Promoter-reporter episomal transfection assays for endothelial nitric oxide synthase, VE-cadherin, and vWF indicated functional promoter activity in cell types where the native gene was not active. Inhibition of DNA methyltransferase activity indicated important functional relevance. Importantly, profiling DNA replication timing patterns indicated that EC-enriched gene promoters with differentially methylated regions replicate early in S-phase in both expressing and nonexpressing cell types. Collectively, these studies highlight the functional importance of promoter DNA methylation in controlling vascular EC gene expression.
    MeSH term(s) Animals ; Antigens, CD/genetics ; Aorta/cytology ; Aorta/metabolism ; Cadherins/genetics ; Cattle ; Cell Adhesion Molecules/genetics ; Cells, Cultured ; Chromatin Immunoprecipitation ; DNA Methylation ; DNA Replication Timing ; Dermis/cytology ; Dermis/metabolism ; Endothelium, Vascular/cytology ; Endothelium, Vascular/metabolism ; Gene Expression Regulation ; Hepatocytes/cytology ; Hepatocytes/metabolism ; Human Umbilical Vein Endothelial Cells/cytology ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Keratinocytes/cytology ; Keratinocytes/metabolism ; Mice ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/metabolism ; Nitric Oxide Synthase Type III/genetics ; Promoter Regions, Genetic/genetics ; S Phase/physiology ; Vascular Endothelial Growth Factor Receptor-1/genetics ; Vascular Endothelial Growth Factor Receptor-2/genetics ; von Willebrand Factor/genetics
    Chemical Substances Antigens, CD ; Cadherins ; Cell Adhesion Molecules ; cadherin 5 ; von Willebrand Factor ; Nitric Oxide Synthase Type III (EC 1.14.13.39) ; Vascular Endothelial Growth Factor Receptor-1 (EC 2.7.10.1) ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
    Language English
    Publishing date 2013-02-28
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood-2013-01-479170
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.140: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 364: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top