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  1. Article ; Online: Drought and Oxidative Stress in Flax (Linum usitatissimum L.) Entails Harnessing Non-Canonical Reference Gene for Precise Quantification of qRT-PCR Gene Expression

    Dash, Prasanta K. / Rai, Rhitu / Pradhan, Sharat Kumar / Shivaraj, Sheelavanta Matha / Deshmukh, Rupesh / Sreevathsa, Rohini / Singh, Nagendra K.

    Antioxidants. 2023 Apr. 18, v. 12, no. 4

    2023  

    Abstract: Flax (Linum usitatissimum L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent. Being a cool (Rabi) season crop, it is affected by unprecedented climatic changes ... ...

    Abstract Flax (Linum usitatissimum L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent. Being a cool (Rabi) season crop, it is affected by unprecedented climatic changes such as high temperature, drought, and associated oxidative stress that, globally, impede its growth, production, and productivity. To precisely assess the imperative changes that are inflicted by drought and associated oxidative stress, gene expression profiling of predominant drought-responsive genes (AREB, DREB/CBF, and ARR) was carried out by qRT-PCR. Nevertheless, for normalization/quantification of data obtained from qRT-PCR results, a stable reference gene is mandatory. Here, we evaluated a panel of four reference genes (Actin, EF1a, ETIF5A, and UBQ) and assessed their suitability as stable reference genes for the normalization of gene expression data obtained during drought-induced oxidative stress in flax. Taking together, from the canonical expression of the proposed reference genes in three different genotypes, we report that EF1a as a stand-alone and EF1a and ETIF5A in tandem are suitable reference genes to be used for the real-time visualization of cellular impact of drought and oxidative stress on flax.
    Keywords Linum usitatissimum ; actin ; diploidy ; drought ; flax ; gene expression ; genes ; oils ; oxidative stress ; self-pollination ; solvents ; temperature
    Language English
    Dates of publication 2023-0418
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12040950
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Drought and Oxidative Stress in Flax (

    Dash, Prasanta K / Rai, Rhitu / Pradhan, Sharat Kumar / Shivaraj, Sheelavanta Matha / Deshmukh, Rupesh / Sreevathsa, Rohini / Singh, Nagendra K

    Antioxidants (Basel, Switzerland)

    2023  Volume 12, Issue 4

    Abstract: Flax ( ...

    Abstract Flax (
    Language English
    Publishing date 2023-04-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12040950
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.

    Rasoolizadeh, Aliyeh / Santhanam, Parthasarathy / Labbé, Caroline / Shivaraj, Sheelavanta Matha / Germain, Hugo / Bélanger, Richard R

    Journal of experimental botany

    2020  Volume 71, Issue 21, Page(s) 6844–6855

    Abstract: In plant-pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host- ... ...

    Abstract In plant-pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host-pathogen interactions, but its mode of action remains a source of debate. Its deposition in the apoplastic area of plant cells suggests that it might interfere with receptor-effector recognition. In this study, soybean plants treated or not with Si were inoculated with Phytophthora sojae and differences in the ensuing infection process were assessed through different microscopy techniques, transcript analysis of effector and defense genes, and effector (Avr6) localization through immunolocalization and fluorescence labeling. In plants grown without Si, the results showed the rapid (4 d post-inoculation) host recognition by P. sojae through the development of haustorium-like bodies, followed by expression and release of effectors into the apoplastic region. In contrast, Si treatment resulted in limited pathogen development, and significantly lower expression and presence of Avr6 in the apoplastic region. Based on immunolocalization and quantification of Avr6 through fluorescence labeling, our results suggest that the presence of Si in the apoplast interferes with host recognition and/or limits receptor-effector interactions, which leads to an incompatible interaction.
    MeSH term(s) Phytophthora ; Plant Diseases ; Plant Proteins/genetics ; Silicon ; Glycine max/genetics
    Chemical Substances Plant Proteins ; Silicon (Z4152N8IUI)
    Language English
    Publishing date 2020-02-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    DOI 10.1093/jxb/eraa101
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch Pathogen

    Yadav, Sagar / Raazi, Zarrine / Shivaraj, Sheelavanta Matha / Somani, Deepika / Prashant, Ramya / Kulkarni, Abhijeet / Kumar, Rajeev / Biradar, Suma / Desai, Shreenivas / Kadoo, Narendra

    Pathogens (Basel, Switzerland)

    2022  Volume 12, Issue 1

    Abstract: Spot blotch is a highly destructive disease in wheat caused by the fungal ... ...

    Abstract Spot blotch is a highly destructive disease in wheat caused by the fungal pathogen
    Language English
    Publishing date 2022-12-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12010001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Si permeability of a deficient Lsi1 aquaporin in tobacco can be enhanced through a conserved residue substitution.

    Coskun, Devrim / Deshmukh, Rupesh / Sonah, Humira / Shivaraj, Sheelavanta Matha / Frenette-Cotton, Rachelle / Tremblay, Laurence / Isenring, Paul / Bélanger, Richard R

    Plant direct

    2019  Volume 3, Issue 8, Page(s) e00163

    Abstract: Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si-permeable Lsi1 (NIP2-1) aquaporin in its roots. Structure- ... ...

    Abstract Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si-permeable Lsi1 (NIP2-1) aquaporin in its roots. Structure-function analyses of Lsi1 channels from higher plants have thus far revealed two key molecular determinants of Si permeability: (a) the amino acid motif GSGR in the aromatic/arginine selectivity filter and (b) 108 amino acids between two highly conserved NPA domains. Curiously, tobacco (
    Language English
    Publishing date 2019-08-21
    Publishing country England
    Document type Journal Article
    ISSN 2475-4455
    ISSN (online) 2475-4455
    DOI 10.1002/pld3.163
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Si permeability of a deficient Lsi1 aquaporin in tobacco can be enhanced through a conserved residue substitution

    Coskun, Devrim / Deshmukh, Rupesh / Sonah, Humira / Shivaraj, Sheelavanta Matha / Frenette‐Cotton, Rachelle / Tremblay, Laurence / Isenring, Paul / Bélanger, Richard R.

    Plant direct. 2019 Aug., v. 3, no. 8

    2019  

    Abstract: Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si‐permeable Lsi1 (NIP2‐1) aquaporin in its roots. Structure‐ ... ...

    Abstract Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si‐permeable Lsi1 (NIP2‐1) aquaporin in its roots. Structure‐function analyses of Lsi1 channels from higher plants have thus far revealed two key molecular determinants of Si permeability: (a) the amino acid motif GSGR in the aromatic/arginine selectivity filter and (b) 108 amino acids between two highly conserved NPA domains. Curiously, tobacco (Nicotiana sylvestris) stands as a rare exception as it possesses an Lsi1 (NsLsi1) with these molecular signatures but is reported as a low Si accumulator. The aim of this study was therefore to identify whether additional determinants influence Si permeability via Lsi1 channels, focusing on the role of residues that differ uniquely in NsLsi1 relative to functional Lsi1 homologs. We observed tobacco indeed absorbed Si poorly (0.1% dw), despite NsLsi1 being expressed constitutively in planta. Si influx measured in NsLsi1‐expressing Xenopus oocytes was very low (<13% that of OsLsi1 from rice (Oryza sativa) over a 3‐hr time course), which likely explains why tobacco is a low Si accumulator. Interestingly, NsLsi1ᴾ¹²⁵F displayed a significant gain of function (threefold increase in Si influx relative to NsLsi1ᵂᵀ), which coincided with a threefold increase in plasma membrane localization in planta, as measured by transient expression of GFP constructs in Nicotiana benthamiana leaves. These findings thus reveal a novel molecular determinant of Si transport in plants and inform breeding, biotechnological, and agricultural practices to effectively utilize Si in the context of plant resilience to environmental stress.
    Keywords Nicotiana benthamiana ; Nicotiana sylvestris ; Oryza sativa ; Xenopus ; aquaporins ; arginine ; gain-of-function mutation ; oocytes ; permeability ; plasma membrane ; rice ; silicon ; tobacco
    Language English
    Dates of publication 2019-08
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ISSN 2475-4455
    DOI 10.1002/pld3.163
    Database NAL-Catalogue (AGRICOLA)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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