Article ; Online: Coordinated changes in glycosylation regulate the germinal center through CD22.
2022 Volume 38, Issue 11, Page(s) 110512
Abstract: Germinal centers (GCs) are essential for antibody affinity maturation. GC B cells have a unique repertoire of cell surface glycans compared with naive B cells, yet functional roles for changes in glycosylation in the GC have yet to be ascribed. Detection ...
| Abstract | Germinal centers (GCs) are essential for antibody affinity maturation. GC B cells have a unique repertoire of cell surface glycans compared with naive B cells, yet functional roles for changes in glycosylation in the GC have yet to be ascribed. Detection of GCs by the antibody GL7 reflects a downregulation in ligands for CD22, an inhibitory co-receptor of the B cell receptor. To test a functional role for downregulation of CD22 ligands in the GC, we generate a mouse model that maintains CD22 ligands on GC B cells. With this model, we demonstrate that glycan remodeling plays a critical role in the maintenance of B cells in the GC. Sustained expression of CD22 ligands induces higher levels of apoptosis in GC B cells, reduces memory B cell and plasma cell output, and delays affinity maturation of antibodies. These defects are CD22 dependent, demonstrating that downregulation of CD22 ligands on B cells plays a critical function in the GC. |
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| MeSH term(s) | Animals ; B-Lymphocytes ; Germinal Center ; Glycosylation ; Ligands ; Mice ; Polysaccharides/metabolism ; Receptors, Antigen, B-Cell/metabolism |
| Chemical Substances | Ligands ; Polysaccharides ; Receptors, Antigen, B-Cell |
| Language | English |
| Publishing date | 2022-03-15 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2649101-1 |
| ISSN | 2211-1247 ; 2211-1247 |
| ISSN (online) | 2211-1247 |
| ISSN | 2211-1247 |
| DOI | 10.1016/j.celrep.2022.110512 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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