Article ; Online: Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing.
STAR protocols
2024 Volume 5, Issue 1, Page(s) 102809
Abstract: Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and ... ...
Abstract | Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and barcode retrieval. We then describe procedures for sequencing library construction while providing options for sample multiplexing and data analysis. This BdLT-Seq technique enables the assessment of clonal evolution in a directional manner while preserving isogeneity, thus allowing the comparison of non-genetic molecular features between isogenic cell lineages. For complete details on the use and execution of this protocol, please refer to Shlyakhtina et al. (2023). |
---|---|
MeSH term(s) | Inheritance Patterns ; Cell Lineage/genetics ; Clonal Evolution ; Cloning, Molecular ; Data Analysis |
Language | English |
Publishing date | 2024-01-04 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 2666-1667 |
ISSN (online) | 2666-1667 |
DOI | 10.1016/j.xpro.2023.102809 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.