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  1. Article: Autoimmune Effect of Antibodies against the SARS-CoV-2 Nucleoprotein

    Matyushkina, Daria / Shokina, Varvara / Tikhonova, Polina / Manuvera, Valentin / Shirokov, Dmitry / Kharlampieva, Daria / Lazarev, Vasily / Varizhuk, Anna / Vedekhina, Tatiana / Pavlenko, Alexander / Penkin, Leonid / Arapidi, Georgij / Pavlov, Konstantin / Pushkar, Dmitry / Kolontarev, Konstantin / Rumyantsev, Alexander / Rumyantsev, Sergey / Rychkova, Lyubov / Govorun, Vadim

    Viruses. 2022 May 25, v. 14, no. 6

    2022  

    Abstract: COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an ... ...

    Abstract COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it is associated with the autoimmune reactions, so debates about the development of new vaccines and the need for vaccination/revaccination continue. In this study we performed an analysis of the antibody response of patients with COVID-19 to linear and conformational epitopes of viral proteins using ELISA, chip array and western blot with analysis of correlations between antibody titer, disease severity, and complications. We have shown that the presence of IgG antibodies to the nucleoprotein can deteriorate the course of the disease, induce multiple direct COVID-19 symptoms, and contribute to long-term post-covid symptoms. We analyzed the cross reactivity of antibodies to SARS-CoV-2 with own human proteins and showed that antibodies to the nucleocapsid protein can bind to human proteins. In accordance with the possibility of HLA presentation, the main possible targets of the autoantibodies were identified. People with HLA alleles A01:01; A26:01; B39:01; B15:01 are most susceptible to the development of autoimmune processes after COVID-19.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; Western blotting ; antibody formation ; autoantibodies ; cross reaction ; disease severity ; epitopes ; humans ; nucleocapsid proteins ; nucleoproteins ; people ; vaccination
    Language English
    Dates of publication 2022-0525
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061141
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Microarray Profiling of Vaccination-Induced Antibody Responses to SARS-CoV-2 Variants of Interest and Concern.

    Svetlova, Julia / Gustin, Dmitry / Manuvera, Valentin / Shirokov, Dmitriy / Shokina, Varvara / Prusakov, Kirill / Aldarov, Konstantin / Kharlampieva, Daria / Matyushkina, Daria / Bespyatykh, Julia / Varizhuk, Anna / Lazarev, Vassili / Vedekhina, Tatiana

    International journal of molecular sciences

    2022  Volume 23, Issue 21

    Abstract: Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines ... ...

    Abstract Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines and justify revaccination, rapid evaluation of antibody (Ab) responses to currently circulating SARS-CoV-2 variants of interest (VOI) and concern (VOC) is needed. Here, we developed a multiplex protein microarray-based system for rapid profiling of anti-SARS-CoV-2 Ab levels in human sera. The microarray system was validated using sera samples from SARS-CoV-2-free donors and those diagnosed with COVID-19 based on PCR and enzyme immunoassays. Microarray-based profiling of vaccinated donors revealed a substantial difference in anti-VOC Ab levels elicited by the replication-deficient adenovirus vector-base (Sputnik V) and whole-virion (CoviVac Russia COVID-19) vaccines. Whole-virion vaccine-induced Abs showed minor but statistically significant cross-reactivity with the human blood coagulation factor 1 (fibrinogen) and thrombin. However, their effects on blood clotting were negligible, according to thrombin time tests, providing evidence against the concept of pronounced cross-reactivity-related side effects of the vaccine. Importantly, all samples were collected in the pre-Omicron period but showed noticeable responses to the receptor-binding domain (RBD) of the Omicron spike protein. Thus, using the new express Ab-profiling system, we confirmed the inter-variant cross-reactivity of the anti-SARS-CoV-2 Abs and demonstrated the relative potency of the vaccines against new VOCs.
    MeSH term(s) Humans ; Antibodies, Neutralizing ; Antibodies, Viral ; Antibody Formation/genetics ; COVID-19/prevention & control ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/genetics ; Vaccination ; Viral Vaccines/genetics ; Viral Vaccines/pharmacology ; COVID-19 Vaccines/genetics ; COVID-19 Vaccines/pharmacology ; Microarray Analysis
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Viral Vaccines ; COVID-19 Vaccines
    Language English
    Publishing date 2022-10-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232113220
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Autoimmune Effect of Antibodies against the SARS-CoV-2 Nucleoprotein.

    Matyushkina, Daria / Shokina, Varvara / Tikhonova, Polina / Manuvera, Valentin / Shirokov, Dmitry / Kharlampieva, Daria / Lazarev, Vasily / Varizhuk, Anna / Vedekhina, Tatiana / Pavlenko, Alexander / Penkin, Leonid / Arapidi, Georgij / Pavlov, Konstantin / Pushkar, Dmitry / Kolontarev, Konstantin / Rumyantsev, Alexander / Rumyantsev, Sergey / Rychkova, Lyubov / Govorun, Vadim

    Viruses

    2022  Volume 14, Issue 6

    Abstract: COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an ... ...

    Abstract COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it is associated with the autoimmune reactions, so debates about the development of new vaccines and the need for vaccination/revaccination continue. In this study we performed an analysis of the antibody response of patients with COVID-19 to linear and conformational epitopes of viral proteins using ELISA, chip array and western blot with analysis of correlations between antibody titer, disease severity, and complications. We have shown that the presence of IgG antibodies to the nucleoprotein can deteriorate the course of the disease, induce multiple direct COVID-19 symptoms, and contribute to long-term post-covid symptoms. We analyzed the cross reactivity of antibodies to SARS-CoV-2 with own human proteins and showed that antibodies to the nucleocapsid protein can bind to human proteins. In accordance with the possibility of HLA presentation, the main possible targets of the autoantibodies were identified. People with HLA alleles A01:01; A26:01; B39:01; B15:01 are most susceptible to the development of autoimmune processes after COVID-19.
    MeSH term(s) Antibodies, Viral ; COVID-19/complications ; Humans ; Nucleoproteins ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Viral ; Nucleoproteins ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-05-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061141
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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