LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Draft Genome Assemblies of Phage AP50c-Resistant Derivatives of Bacillus anthracis Sterne Strain 7702 Lacking Plasmid pXO2.

    Sholes, Samantha L / Harrison, Sarah / Forrest, Samantha / Ton, Sarah / Grady, Sarah L / Verratti, Kathleen / Plaut, Roger D / Goodwin, Bruce G / Sozhamannan, Shanmuga

    Microbiology resource announcements

    2023  Volume 12, Issue 2, Page(s) e0131322

    Abstract: Mutants of the attenuated Bacillus anthracis (Sterne) strain 7702 that are resistant to phage AP50c have been previously described. Here, we report the draft genome assemblies of the parent strain, several phage-resistant derivatives, and mutants of ... ...

    Abstract Mutants of the attenuated Bacillus anthracis (Sterne) strain 7702 that are resistant to phage AP50c have been previously described. Here, we report the draft genome assemblies of the parent strain, several phage-resistant derivatives, and mutants of genes in the pathways for synthesis and assembly of the S-layer.
    Language English
    Publishing date 2023-01-31
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.01313-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Human telomere length is chromosome specific and conserved across individuals.

    Karimian, Kayarash / Groot, Aljona / Huso, Vienna / Kahidi, Ramin / Tan, Kar-Tong / Sholes, Samantha / Keener, Rebecca / McDyer, John F / Alder, Jonathan K / Li, Heng / Rechtsteiner, Andreas / Greider, Carol W

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Short telomeres cause age-related disease and long telomeres predispose to cancer; however, the mechanisms regulating telomere length are unclear. To probe these mechanisms, we developed a nanopore sequencing method, Telomere Profiling, that is easy to ... ...

    Abstract Short telomeres cause age-related disease and long telomeres predispose to cancer; however, the mechanisms regulating telomere length are unclear. To probe these mechanisms, we developed a nanopore sequencing method, Telomere Profiling, that is easy to implement, precise, and cost effective with broad applications in research and the clinic. We sequenced telomeres from individuals with short telomere syndromes and found similar telomere lengths to the clinical FlowFISH assay. We mapped telomere reads to specific chromosome end and identified both chromosome end-specific and haplotype-specific telomere length distributions. In the T2T HG002 genome, where the average telomere length is 5kb, we found a remarkable 6kb difference in lengths between some telomeres. Further, we found that specific chromosome ends were consistently shorter or longer than the average length across 147 individuals. The presence of conserved chromosome end-specific telomere lengths suggests there are new paradigms in telomere biology that are yet to be explored. Understanding the mechanisms regulating length will allow deeper insights into telomere biology that can lead to new approaches to disease.
    Language English
    Publishing date 2024-01-13
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.12.21.572870
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Human telomere length is chromosome end-specific and conserved across individuals.

    Karimian, Kayarash / Groot, Aljona / Huso, Vienna / Kahidi, Ramin / Tan, Kar-Tong / Sholes, Samantha / Keener, Rebecca / McDyer, John F / Alder, Jonathan K / Li, Heng / Rechtsteiner, Andreas / Greider, Carol W

    Science (New York, N.Y.)

    2024  Volume 384, Issue 6695, Page(s) 533–539

    Abstract: Short telomeres cause age-related disease, and long telomeres contribute to cancer; however, the mechanisms regulating telomere length are unclear. We developed a nanopore-based method, which we call Telomere Profiling, to determine telomere length at ... ...

    Abstract Short telomeres cause age-related disease, and long telomeres contribute to cancer; however, the mechanisms regulating telomere length are unclear. We developed a nanopore-based method, which we call Telomere Profiling, to determine telomere length at nearly single-nucleotide resolution. Mapping telomere reads to chromosome ends showed chromosome end-specific length distributions that could differ by more than six kilobases. Examination of telomere lengths in 147 individuals revealed that certain chromosome ends were consistently longer or shorter. The same rank order was found in newborn cord blood, suggesting that telomere length is determined at birth and that chromosome end-specific telomere length differences are maintained as telomeres shorten with age. Telomere Profiling makes precision investigation of telomere length widely accessible for laboratory, clinical, and drug discovery efforts and will allow deeper insights into telomere biology.
    MeSH term(s) Humans ; Telomere/genetics ; Infant, Newborn ; Fetal Blood ; Telomere Homeostasis ; Telomere Shortening ; Chromosomes, Human/genetics ; Nanopore Sequencing/methods ; Male ; Adult
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.ado0431
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 27: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 77: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing.

    Sholes, Samantha L / Karimian, Kayarash / Gershman, Ariel / Kelly, Thomas J / Timp, Winston / Greider, Carol W

    Genome research

    2021  Volume 32, Issue 4, Page(s) 616–628

    Abstract: We developed a method to tag telomeres and measure telomere length by nanopore sequencing in the ... ...

    Abstract We developed a method to tag telomeres and measure telomere length by nanopore sequencing in the yeast
    MeSH term(s) Intracellular Signaling Peptides and Proteins/genetics ; Nanopore Sequencing ; Protein Serine-Threonine Kinases ; Repressor Proteins/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Telomerase/genetics ; Telomerase/metabolism ; Telomere/genetics ; Telomere/metabolism ; Telomere-Binding Proteins/genetics ; Telomere-Binding Proteins/metabolism
    Chemical Substances Intracellular Signaling Peptides and Proteins ; Repressor Proteins ; Saccharomyces cerevisiae Proteins ; Telomere-Binding Proteins ; RIF1 protein, S cerevisiae (146589-82-4) ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; TEL1 protein, S cerevisiae (EC 2.7.11.1) ; Telomerase (EC 2.7.7.49)
    Language English
    Publishing date 2021-10-26
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1284872-4
    ISSN 1549-5469 ; 1088-9051 ; 1054-9803
    ISSN (online) 1549-5469
    ISSN 1088-9051 ; 1054-9803
    DOI 10.1101/gr.275868.121
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3729: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 8: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Genetic evidence for the interaction between

    Forrest, Samantha / Ton, Sarah / Sholes, Samantha L / Harrison, Sarah / Plaut, Roger D / Verratti, Kathleen / Wittekind, Michael / Ettehadieh, Elham / Necciai, Bryan / Sozhamannan, Shanmuga / Grady, Sarah L

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1278791

    Abstract: Bacteriophages such as γ and AP50c have been shown to infect strains ... ...

    Abstract Bacteriophages such as γ and AP50c have been shown to infect strains of
    Language English
    Publishing date 2023-10-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1278791
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: The importance of Ile716 toward the mutagenicity of 8-Oxo-2'-deoxyguanosine with Bacillus fragment DNA polymerase.

    Hamm, Michelle L / Garcia, Anarosa A / Gilbert, Rachel / Johri, Manavi / Ricart, Miranda / Sholes, Samantha L / Murray-Nerger, Laura A / Wu, Eugene Y

    DNA repair

    2020  Volume 89, Page(s) 102826

    Abstract: 8-oxo-2'-deoxyguanosine (OdG) is a prominent DNA lesion that can direct the incorporation of dCTP or dATP during replication. As the latter reaction can lead to mutation, the ratio of dCTP/dATP incorporation can significantly affect the mutagenic ... ...

    Abstract 8-oxo-2'-deoxyguanosine (OdG) is a prominent DNA lesion that can direct the incorporation of dCTP or dATP during replication. As the latter reaction can lead to mutation, the ratio of dCTP/dATP incorporation can significantly affect the mutagenic potential of OdG. Previous work with the A-family polymerase BF and seven analogues of OdG identified a major groove amino acid, Ile716, which likely influences the dCTP/dATP incorporation ratio opposite OdG. To further probe the importance of this amino acid, dCTP and dATP incorporations opposite the same seven analogues were tested with two BF mutants, I716M and I716A. Results from these studies support the presence of clashing interactions between Ile716 and the C8-oxygen and C2-amine during dCTP and dATP incorporations, respectively. Crystallographic analysis suggests that residue 716 alters the conformation of the template base prior to insertion into the active site, thereby affecting enzymatic efficiency. These results are also consistent with previous work with A-family polymerases, which indicate they have tight, rigid active sites that are sensitive to template perturbations.
    MeSH term(s) 8-Hydroxy-2'-Deoxyguanosine/metabolism ; Bacillus/enzymology ; Crystallography ; DNA-Directed DNA Polymerase/genetics ; DNA-Directed DNA Polymerase/metabolism ; Models, Molecular ; Molecular Conformation ; Mutagens/metabolism ; Mutation ; Protein Conformation
    Chemical Substances Mutagens ; 8-Hydroxy-2'-Deoxyguanosine (88847-89-6) ; DNA-Directed DNA Polymerase (EC 2.7.7.7)
    Language English
    Publishing date 2020-02-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2071608-4
    ISSN 1568-7856 ; 1568-7864
    ISSN (online) 1568-7856
    ISSN 1568-7864
    DOI 10.1016/j.dnarep.2020.102826
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5555: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top