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Article: Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report.

Chen, Shu-Yi / Chen, Qun-Wei / Shou, Liu-Mei / Pan, Hong / Ruan, Shan-Ming / Liang, Zhe-Hao / Shu, Qi-Jin

2021 Volume 19, Issue 6, Page(s) 555–560

Abstract: Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) is a rare adverse cutaneous reaction with a low incidence and high mortality. Despite posing a serious threat to patients' health and lives, there is no high-quality evidence for a standard ... ...

Abstract	Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) is a rare adverse cutaneous reaction with a low incidence and high mortality. Despite posing a serious threat to patients' health and lives, there is no high-quality evidence for a standard treatment regimen. Here we report the case of a 62-year-old man with stage IV pancreatic cancer who experienced immunotherapy-induced SJS/TEN. After consensus-based regular treatments at a local hospital, his symptoms became worse. Thus, he consented to receive Chinese herbal medicine (CHM) therapy. The affected parts of the patient were treated with the CHM Pi-Yan-Ning which was applied externally for 20 min twice a day. After 7 days of treatment, the dead skin began peeling away from the former lesions that had covered his hands, feet, and lips, indicating that skin had regenerated. After 12 days of treatment, the patient's skin was completely recovered. In this case, SJS/TEN was successfully treated with Pi-Yan-Ning, suggesting that there might be tremendous potential for the use of Pi-Yan-Ning in the treatment of severe skin reactions to drug treatments. Further basic investigations and clinical trials to explore the mechanism and efficacy are needed.
MeSH term(s)	Drugs, Chinese Herbal/therapeutic use ; Humans ; Immunologic Factors ; Incidence ; Male ; Middle Aged ; Skin ; Stevens-Johnson Syndrome/drug therapy ; Stevens-Johnson Syndrome/etiology
Chemical Substances	Drugs, Chinese Herbal ; Immunologic Factors
Language	English
Publishing date	2021-10-11
Publishing country	Netherlands
Document type	Case Reports
ZDB-ID	2705612-0
ISSN	2095-4964
ISSN	2095-4964
DOI	10.1016/j.joim.2021.10.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Values of applying white blood cell counts in the prognostic evaluation of resectable colorectal cancer.

Wu, Jing / Ge, Xin-Xin / Zhu, Wenyu / Zhi, Qiaoming / Xu, Meng-Dan / Duan, Weiming / Chen, Kai / Gong, Fei-Ran / Tao, Min / Shou, Liu-Mei / Wu, Meng-Yao / Wang, Wen-Jie

Molecular medicine reports

2019 Volume 19, Issue 3, Page(s) 2330–2340

Abstract: The count and classification of white blood cells (WBCs) may be used as prognostic markers in certain types of cancer. The present study investigated the prognostic potential of the counts of WBCs, including lymphocytes (LYs), monocytes (MOs), ... ...

Abstract	The count and classification of white blood cells (WBCs) may be used as prognostic markers in certain types of cancer. The present study investigated the prognostic potential of the counts of WBCs, including lymphocytes (LYs), monocytes (MOs), neutrophils (NEs), eosinophils (EOs) and basophils (BAs), in the prognosis of resectable colorectal cancer. The present study recruited 153 resectable colorectal cancer cases retrospectively, which were pathologically confirmed. All patients were divided into two groups, according to the median value of LY (low LY, ≤1.632x109/l or high LY, >1.632x109/l), MO (low MO, ≤0.330x109/l or high MO, >0.330x109/l), NE (low NE, ≤3.600x109/l or high NE, >3.600x109/l), EO (low EO, ≤0.085x109/l or high EO, >0.085x109/l), BA (low BA, ≤0.010x109/l or high BA, >0.010x109/l), or WBC (low WBC, ≤5.780x109/l or high WBC, >5.780x109/l). To evaluate the alterations in WBC counts following surgery and adjuvant chemotherapy; all samples received oxiplatin and capecitabine (XELOX) for 6‑8 cycles or 5‑fluorouracil, leucovorin and oxaliplatin (mFOLFOX6) for 10‑12 cycles. XELOX included oxaliplatin administered intravenously at a dose of 130 mg/m2 on day 1 and 850‑1,250 mg/m2 capecitabine twice daily for days 1‑14, repeated every 3 weeks. mFOLFOX6 included oxaliplatin administered intravenously at a dose of 85 mg/m2, 400 mg/m2 leucovorin and 400 mg/m2 5‑FU on day 1 followed by 1,200 mg/m2/days continuous infusion for 2 days (in total, 2,400 mg/m2 over 46‑48 h), repeated every 2 weeks. The present study investigated the post/pre‑treatment of LY, MO, NE, EO, BA and WBC ratios (≤1 indicated that LY, MO, NE, EO, BA and WBC counts were not increased following therapy; whereas, >1 suggested increased counts). Kaplan‑Meier curves were constructed to demonstrate overall survival (OS). A multivariate and univariate logistic regression analyses model was employed to identify the independent risk factors. Low pre‑treatment BA counts were associated with larger tumor size (>5 cm); pre‑treatment BA levels were positively associated with OS. Surgery significantly decreased the count of BAs and increased the count of EOs; whereas, no effect was observed on LYs, MOs, NEs or WBCs. Adjuvant chemotherapy markedly decreased the counts of LY, NE and WBC; whereas, no notable effects on MOs, EOs or BAs were observed. Whole course treatment (surgery combined with adjuvant chemotherapy) significantly decreased the values of LY, NE and WBC; however, increased the value of EO; no effects on the MO or BA counts were observed. An increased post‑/pre‑treatment NE ratio suggested poorer prognosis. Multivariate Cox regression analysis revealed that sex, tumor size, pre‑treatment BA count and the post‑/pre‑treatment NE ratio were independent prognostic factors affecting OS. The results of the present study suggested that the pre‑treatment BA count and post‑/pre‑treatment NE ratio may be potential prognostic factors for resectable colorectal cancer.
MeSH term(s)	Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols/administration & dosage ; Basophils/drug effects ; Capecitabine ; Chemotherapy, Adjuvant ; Colorectal Neoplasms/blood ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/pathology ; Deoxycytidine/administration & dosage ; Deoxycytidine/analogs & derivatives ; Eosinophils/drug effects ; Female ; Fluorouracil/administration & dosage ; Fluorouracil/analogs & derivatives ; Humans ; Leucovorin/administration & dosage ; Leukocyte Count ; Lymphocytes/drug effects ; Male ; Middle Aged ; Neutrophils/drug effects ; Organoplatinum Compounds/administration & dosage ; Oxaloacetates ; Prognosis
Chemical Substances	Organoplatinum Compounds ; Oxaloacetates ; Deoxycytidine (0W860991D6) ; Capecitabine (6804DJ8Z9U) ; Leucovorin (Q573I9DVLP) ; Fluorouracil (U3P01618RT)
Language	English
Publishing date	2019-01-10
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2469505-1
ISSN	1791-3004 ; 1791-2997
ISSN (online)	1791-3004
ISSN	1791-2997
DOI	10.3892/mmr.2019.9844
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Prognostic Values of Platelet-Associated Indicators in Resectable Lung Cancers.

Wang, Jing-Jing / Wang, Yin-Ling / Ge, Xin-Xin / Xu, Meng-Dan / Chen, Kai / Wu, Meng-Yao / Gong, Fei-Ran / Tao, Min / Wang, Wen-Jie / Shou, Liu-Mei / Li, Wei

Technology in cancer research & treatment

2019 Volume 18, Page(s) 1533033819837261

Abstract: Background: Lung cancer is the leading cause of cancer death. Platelet-related indictors, including platelet count, plateletcrit, mean platelet volume, and platelet distribution width, not only associate with morphology and functions of platelet but ... ...

Abstract	Background: Lung cancer is the leading cause of cancer death. Platelet-related indictors, including platelet count, plateletcrit, mean platelet volume, and platelet distribution width, not only associate with morphology and functions of platelet but also correlate with tumor development and metastasis. In the present study, we investigated the values of platelet-related indictors in the prognosis evaluation of resectable lung cancers. Methods: In total, 101 patients with resectable lung cancer were recruited in this study. Patients were divided into 2 groups according to the median pretreatment values. To evaluate the individual value changes after treatment, we introduced the concept of post-/pretreatment ratio (≤1 indicated value was not increased after treatment, while >1 suggested increased value). Results: The high pretreatment platelet count level was correlated with larger tumor size. High pretreatment plateletcrit level was associated with more lymph nodes metastasis. Patients with high pretreatment plateletcrit level had worse overall survival, whereas pretreatment platelet count, mean platelet volume, and platelet distribution width levels were not correlated with outcomes. Surgery had no impact on the values of platelet count, plateletcrit, mean platelet volume, or platelet distribution width. Adjuvant chemotherapy significantly decreased the values of platelet count and plateletcrit, whereas it had no effect on the values of mean platelet volume or platelet distribution width. Whole course of treatment (surgery combined with adjuvant chemotherapy) significantly decreased the values of platelet count and platelet distribution width, whereas it had no effect on the values of plateletcrit or mean platelet volume. Post-/pretreatment platelet count, plateletcrit, mean platelet volume, and platelet distribution width ratios were not correlated with outcomes. Univariate analyses demonstrated that American Joint Committee on Cancer stage and pretreatment plateletcrit level were significant risk factors for prognosis. Cox regression analysis revealed that no factor independently associated with worse survival. Conclusion: Pretreatment plateletcrit level could be a potential prognostic factor in resectable lung cancers.
MeSH term(s)	Blood Platelets/metabolism ; Blood Platelets/pathology ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/secondary ; Carcinoma, Non-Small-Cell Lung/surgery ; Female ; Follow-Up Studies ; Humans ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Lung Neoplasms/surgery ; Lymphatic Metastasis ; Male ; Mean Platelet Volume ; Middle Aged ; Neoplasm Invasiveness ; Prognosis ; Retrospective Studies ; Small Cell Lung Carcinoma/metabolism ; Small Cell Lung Carcinoma/secondary ; Small Cell Lung Carcinoma/surgery ; Survival Rate
Language	English
Publishing date	2019-03-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2146365-7
ISSN	1533-0338 ; 1533-0346
ISSN (online)	1533-0338
ISSN	1533-0346
DOI	10.1177/1533033819837261
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The combination of cantharidin and antiangiogenic therapeutics presents additive antitumor effects against pancreatic cancer.

Xu, Meng-Dan / Liu, Lu / Wu, Meng-Yao / Jiang, Min / Shou, Liu-Mei / Wang, Wen-Jie / Wu, Jing / Zhang, Yan / Gong, Fei-Ran / Chen, Kai / Tao, Min / Zhi, Qiaoming / Li, Wei

Oncogenesis

2018 Volume 7, Issue 11, Page(s) 94

Abstract: Cantharidin, one of the active components of mylabris, is believed to have antitumor activity. Cantharidin selectively inhibits protein phosphatase 2A (PP2A), which can repress multiple oncogenic kinases (ERK, JNK, PKC, and NF-κB). Researches in vitro ... ...

Abstract	Cantharidin, one of the active components of mylabris, is believed to have antitumor activity. Cantharidin selectively inhibits protein phosphatase 2A (PP2A), which can repress multiple oncogenic kinases (ERK, JNK, PKC, and NF-κB). Researches in vitro have shown that cantharidin suppresses cell viability and metastasis in pancreatic cancer cells. This study aims to investigate the effects of cantharidin on pancreatic cancer xenografts in vivo. Xenograft models were established using cells stably expressing luciferase. Xenograft growth was evaluated by living imaging. Gene expression was determined using a microarray, real-time PCR, a RayBiotech antibody array, and the Milliplex assay. Surprisingly, cantharidin significantly accelerated xenograft growth. Living imaging showed a rapid distribution of D-luciferin in cantharidin-treated xenografts, suggesting a rich blood supply. Immunohistochemistry confirmed increased angiogenesis. Microarray and antibody array identified upregulated proangiogenic and downregulated antiangiogenic factors. The Milliplex assay suggested elevated secretion of IL-6, IL-8, TNF-α, and VEGF. Inhibitors of ERK, JNK, PKC, and NF-κB pathway attenuated the cantharidin-induced changes to proangiogenic gene expression. PKC pathway-inhibiting tamoxifen or antiangiogenic therapeutics, including Ginsenoside Rg3, bevacizumab, Apatinib, and Endostar, antagonized the proangiogenic effect of cantharidin or its derivatives. These regimens presented remarkable additive antitumor effects in vivo. Although cantharidin presents antitumor effects in vitro and has been applied in clinical practice, we revealed an unfavorable proangiogenic side effect. We recommend that the clinical application of cantharidin should be performed on the premise of antivascularization therapy.
Language	English
Publishing date	2018-11-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2674437-5
ISSN	2157-9024
ISSN	2157-9024
DOI	10.1038/s41389-018-0102-2
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Article ; Online: The values of applying classification and counts of white blood cells to the prognostic evaluation of resectable gastric cancers.

Wang, Yin-Ling / Ge, Xin-Xin / Wang, Yi / Xu, Meng-Dan / Gong, Fei-Ran / Tao, Min / Wang, Wen-Jie / Shou, Liu-Mei / Chen, Kai / Wu, Meng-Yao / Li, Wei

BMC gastroenterology

2018 Volume 18, Issue 1, Page(s) 99

Abstract: Background: The classifications and counts of white blood cells (WBCs) have been proved to be able to be used as prognostic markers in cancer cases. The present study investigated the potential values of the classifications and counts of WBC, including ... ...

Abstract	Background: The classifications and counts of white blood cells (WBCs) have been proved to be able to be used as prognostic markers in cancer cases. The present study investigated the potential values of the classifications and counts of WBC, including lymphocyte (LY), monocyte (MO), neutrophil (NE), eosinophil (EO), and basophil (BA) in the prognosis of resectable gastric cancers (GCs). Methods: This retrospective study recruited 104 resectable GC cases which were pathologically confirmed. The patients were divided into two groups according to the median pre-treatment values. To evaluate the changes in WBC counts and classification after treatment, we introduced the concept of post/pre-treatment ratios (≤ 1 indicated count was not increased after therapy, while > 1 suggested increased count). Results: Pre-treatment NE and total WBC counts were negatively correlated with overall survival (OS). Surgery significantly decreased the level of NE count, but increased the level of EO, whereas had no effect on the levels of LY, MO, BAor total WBC. Adjuvant chemotherapy significantly decreased the level of BA. Whole course of treatment (surgery combined with adjuvant chemotherapy) had no significant effect on the counts of LY, MO, NE, EO, BA or total WBC. Post/pre-treatment ratios of LY, MO NE, EO, BA and total WBC levels had no effects on OS. Univariate analysis indicated that AJCC stage (III) and higher level of pre-treatment total WBC count were prognostic factors affecting OS. Multivariate Cox regression analysis revealed that AJCC stage (III) and higher level of pre-treatment total WBC count were independent prognostic factors. Conclusions: Pre-treatment NE count and pre-treatment total WBC count may be potential prognostic factors for the prognostic evaluation of GCs.
MeSH term(s)	Adult ; Aged ; Chemotherapy, Adjuvant ; Female ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Stomach Neoplasms/classification ; Stomach Neoplasms/immunology ; Stomach Neoplasms/pathology ; Stomach Neoplasms/surgery ; Survival Analysis
Language	English
Publishing date	2018-06-28
Publishing country	England
Document type	Journal Article
ISSN	1471-230X
ISSN (online)	1471-230X
DOI	10.1186/s12876-018-0812-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Artesunate induces G2/M cell cycle arrest through autophagy induction in breast cancer cells.

Chen, Kai / Shou, Liu-Mei / Lin, Fang / Duan, Wei-Ming / Wu, Meng-Yao / Xie, Xin / Xie, Yu-Feng / Li, Wei / Tao, Min

Anti-cancer drugs

2014 Volume 25, Issue 6, Page(s) 652–662

Abstract: We found that artesunate (ART) inhibited the growth of MCF-7 and MDA-MB-231 breast cancer cells. ART arrested the cell cycle in the G2/M phase, which was accompanied by an upregulation of p21. ART upregulated the expression of Beclin1, an initiator of ... ...

Abstract	We found that artesunate (ART) inhibited the growth of MCF-7 and MDA-MB-231 breast cancer cells. ART arrested the cell cycle in the G2/M phase, which was accompanied by an upregulation of p21. ART upregulated the expression of Beclin1, an initiator of autophagy (type II programmed cell death). In addition, ART stimulated the aggregation of LC3, which is considered to be a marker of autophagosome formation. We further verified the transformation of LC3 from type I into type II. 3-MA, a classical autophagy inhibitor, attenuated ART-induced autophagosome formation, cell growth repression, G2/M arrest, and p21 upregulation. Autophagy induction and p21 upregulation were also repressed by knockdown of Beclin1. Furthermore, ART sensitized breast cancer cells to the chemotherapeutic agent epirubicin through an autophagy-dependent cascade. Our study showed that ART induced autophagy in breast cancer cells and indicated that the anticancer effects of ART were exerted through an autophagy pathway. Moreover, ART sensitized breast cancer cells to epirubicin chemotherapy. Our results provide a basis for further development of ART as a novel therapeutic agent for the treatment of breast cancer.
MeSH term(s)	Antineoplastic Agents/pharmacology ; Apoptosis Regulatory Proteins/metabolism ; Artemisinins/pharmacology ; Autophagy/drug effects ; Beclin-1 ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Drug Synergism ; Epirubicin/pharmacology ; Female ; G2 Phase Cell Cycle Checkpoints/drug effects ; Humans ; Membrane Proteins/metabolism
Chemical Substances	Antineoplastic Agents ; Apoptosis Regulatory Proteins ; Artemisinins ; BECN1 protein, human ; Beclin-1 ; Membrane Proteins ; Epirubicin (3Z8479ZZ5X) ; artesunate (60W3249T9M)
Language	English
Publishing date	2014-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1065301-6
ISSN	1473-5741 ; 0959-4973
ISSN (online)	1473-5741
ISSN	0959-4973
DOI	10.1097/CAD.0000000000000089
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3125: Show issues

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Article: TNF-α sensitizes chemotherapy and radiotherapy against breast cancer cells.

Wu, Xiao / Wu, Meng-Yao / Jiang, Min / Zhi, Qiaoming / Bian, Xiaojie / Xu, Meng-Dan / Gong, Fei-Ran / Hou, Juan / Tao, Min / Shou, Liu-Mei / Duan, Weiming / Chen, Kai / Shen, Meng / Li, Wei

Cancer cell international

2017 Volume 17, Page(s) 13

Abstract: Purpose: Despite new developments in cancer therapy, chemotherapy and radiotherapy remain the cornerstone of breast cancer treatment. Therefore, finding ways to reduce the toxicity and increase sensitivity is particularly important. Tumor necrosis ... ...

Abstract	Purpose: Despite new developments in cancer therapy, chemotherapy and radiotherapy remain the cornerstone of breast cancer treatment. Therefore, finding ways to reduce the toxicity and increase sensitivity is particularly important. Tumor necrosis factor alpha (TNF-α) exerts multiple functions in cell proliferation, differentiation and apoptosis. In the present study, we investigated whether TNF-α could enhance the effect of chemotherapy and radiotherapy against breast cancer cells. Methods: Cell growth was determined by MTT assay in vitro, and by using nude mouse tumor xenograft model in vivo. Cell cycle and apoptosis/necrosis were evaluated by flow cytometry. DNA damage was visualized by phospho-Histone H2A.X staining. mRNA expression was assessed by using real-time PCR. Protein expression was tested by Western blot assay. Results: TNF-α strengthened the cytotoxicity of docetaxel, 5-FU and cisplatin against breast cancer cells both in vitro and in vivo. TNF-α activated NF-κB pathway and dependently up-regulated expressions of CyclinD1, CyclinD2, CyclinE, CDK2, CDK4 and CDK6, the key regulators participating in G1→S phase transition. As a result, TNF-α drove cells out of quiescent G0/G1 phase, entering vulnerable proliferating phases. Treatment of TNF-α brought more DNA damage after Cs Conclusion: TNF-α presented radiotherapy- and chemotherapy-sensitizing effects against breast cancer cells.
Language	English
Publishing date	2017-01-23
Publishing country	England
Document type	Journal Article
ISSN	1475-2867
ISSN	1475-2867
DOI	10.1186/s12935-017-0382-1
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Article ; Online: Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after in-solution hybrid capture.

Xu, Meng-Dan / Liu, Shu-Ling / Feng, Yi-Zhong / Liu, Qiang / Shen, Meng / Zhi, Qiaoming / Liu, Zeyi / Gu, Dong-Mei / Yu, Jie / Shou, Liu-Mei / Gong, Fei-Ran / Zhu, Qi / Duan, Weiming / Chen, Kai / Zhang, Junning / Wu, Meng-Yao / Tao, Min / Li, Wei

Oncotarget

2017 Volume 8, Issue 9, Page(s) 14620–14635

Abstract: Squamous cell carcinoma (SCC) of pancreas is a rare histotype of pancreatic ductal carcinoma which is distinct from pancreatic adenocarcinoma (AC). Although there are standard treatments for pancreatic AC, no precise therapies exist for pancreatic SCC. ... ...

Abstract	Squamous cell carcinoma (SCC) of pancreas is a rare histotype of pancreatic ductal carcinoma which is distinct from pancreatic adenocarcinoma (AC). Although there are standard treatments for pancreatic AC, no precise therapies exist for pancreatic SCC. Here, we screened 1033 cases of pancreatic cancer and identified 2 cases of pure SCC, which were pathologically diagnosed on the basis of finding definite intercellular bridges and/or focal keratin peal formation in the tumor cells. Immunohistochemistry assay confirmed the positive expression of CK5/6 and p63 in pancreatic SCC. To verify the genomic characteristics of pancreatic SCC, we employed in-solution hybrid capture targeting 137 cancer-related genes accompanied by high throughput sequencing (HTS) to compare the different genetic variants in SCC and AC of pancreas. We compared the genetic alterations of known biomarkers of pancreatic adenocarcinoma in different pancreatic cancer tissues, and identified nine mutated genes in SCC of pancreas: C7orf70, DNHD1, KPRP, MDM4, MUC6, OR51Q1, PTPRD, TCF4, TET2, and nine genes (ABCB1, CSF1R, CYP2C18, FBXW7, ITPA, KIAA0748, SOD2, SULT1A2, ZNF142) that are mutated in pancreatic AC. This study may have taken one step forward on the discovery of potential biomarkers for the targeted treatment of SCC of the pancreas.
MeSH term(s)	Adenocarcinoma/diagnosis ; Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Carcinoma, Squamous Cell/diagnosis ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/metabolism ; Diagnosis, Differential ; Gene Expression Regulation, Neoplastic ; Gene Ontology ; Genomics/methods ; High-Throughput Nucleotide Sequencing/methods ; Humans ; INDEL Mutation ; Immunohistochemistry ; Keratin-5/metabolism ; Keratin-6/metabolism ; Mutation ; Pancreatic Neoplasms/diagnosis ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/metabolism ; Polymorphism, Single Nucleotide ; Transcription Factors/metabolism ; Tumor Suppressor Proteins/metabolism
Chemical Substances	Biomarkers, Tumor ; Keratin-5 ; Keratin-6 ; TP63 protein, human ; Transcription Factors ; Tumor Suppressor Proteins
Language	English
Publishing date	2017-02-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.14678
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The association between expressions of Ras and CD68 in the angiogenesis of breast cancers.

Li, Wei / Liang, Rong-Rui / Zhou, Chong / Wu, Meng-Yao / Lian, Lian / Yuan, Gao-Feng / Wang, Ming-Yun / Xie, Xin / Shou, Liu-Mei / Gong, Fei-Ran / Chen, Kai / Duan, Wei-Ming / Tao, Min

Cancer cell international

2015 Volume 15, Issue 1, Page(s) 17

Abstract: Objective: Angiogenesis is a critical step of breast cancer metastasis. Oncogenic Ras promotes the remodeling of cancer microenviroment. Tumor-associated macrophages (TAMs) are a prominent inflammatory cell population emerging in the microenviroment and ...

Abstract	Objective: Angiogenesis is a critical step of breast cancer metastasis. Oncogenic Ras promotes the remodeling of cancer microenviroment. Tumor-associated macrophages (TAMs) are a prominent inflammatory cell population emerging in the microenviroment and facilitating the angiogenesis and metastasis. In the present study, we tried to investigate the relationship between the expression of Ras and infiltration of TAM, both of which could further promote angiogenesis. Methods: Expressions of Ras, CD68 and CD34 were assessed by immunohistochemistry. The infiltration of macrophages was evaluated by counting the number of CD68(+) cells. Vessel endothelial cells were defined as CD34(+) cells. Angiogenesis vascularity was defined by microvessel density (MVD) assay through counting the number of vessels per field counted in the area of highest vascular density. The Kaplan-Meier survival analysis was used to estimate the overall survival (OS). Macrophages were derived from monocytes in the presence of macrophage colony-stimulating-factor (MCSF). Breast cancer cells were treated with macrophage-conditioned medium (MCM) and tested the expressions of K-, H- and N-Ras by using realtime-PCR. Results: Ras positive status was correlated with ER, PR and Her-2 positivity, larger tumour size and lymph node metastasis, as well as higher TNM stages. A higher number of CD68(+) cells was correlated with larger tumour size, higher TNM stages and Her-2 positivity. Both Ras positivity and infiltration of CD68(+) macrophages correlated with poor OS. The number of CD68(+) cells was positively correlated with the expression of Ras. Treatment with MCM did not up-regulate but repressed the expression of Ras. Both up-regulation of Ras and infiltration of TAMs correlated with increased MVD. Conclusion: Expression of Ras and infiltration of TAM were positively correlated, and both participated in angiogenesis. Elevated Ras could be responsible for the infiltration of TAM.
Language	English
Publishing date	2015-02-07
Publishing country	England
Document type	Journal Article
ISSN	1475-2867
ISSN	1475-2867
DOI	10.1186/s12935-015-0169-1
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Article: Tamoxifen enhances the anticancer effect of cantharidin and norcantharidin in pancreatic cancer cell lines through inhibition of the protein kinase C signaling pathway.

Xie, Xin / Wu, Meng-Yao / Shou, Liu-Mei / Chen, Long-Pei / Gong, Fei-Ran / Chen, Kai / Li, Dao-Ming / Duan, Wei-Ming / Xie, Yu-Feng / Mao, Yi-Xiang / Li, Wei / Tao, Min

Oncology letters

2014 Volume 9, Issue 2, Page(s) 837–844

Abstract: Cantharidin is an active constituent of mylabris, a traditional Chinese therapeutic agent. Cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A). Cantharidin has been previously reported to efficiently repress the growth of ... ...

Abstract	Cantharidin is an active constituent of mylabris, a traditional Chinese therapeutic agent. Cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A). Cantharidin has been previously reported to efficiently repress the growth of pancreatic cancer cells. However, excessively activated protein kinase C (PKC) has been shown to improve cell survival following the adminstration of cantharidin. Tamoxifen is widely used in the treatment of estrogen receptor-positive breast cancer. In addition, an increasing number of studies have found that tamoxifen selectively inhibits PKC and represses growth in estrogen receptor-negative cancer cells. Administration of a combination of PKC inhibitor and PP2A inhibitors has been demonstrated to exert a synergistic anticancer effect. The proliferation of pancreatic cancer cells was analyzed by 3-(4,5-dimethyltiazol-2-yl]2, 5-diphenyltetrazo-lium bromide assay. The expression levels of ERα and ERβ in various pancreatic cancer cell lines were determined by reverse transcription polymerase chain reaction. In addition, the protein levels of PKCα and phosphorylated PKCα in pancreatic cell lines were analyzed by western blot analysis. In the present study, tamoxifen was found to exert a cytotoxic effect against pancreatic cancer cells independent of the hormone receptor status. Tamoxifen repressed the phosphorylation of PKC, and amplified the anticancer effect induced by cantharidin and norcantharidin. The findings reveal a novel potential strategy against pancreatic cancer using co-treatment with tamoxifen plus cantharidin or cantharidin derivatives.
Language	English
Publishing date	2014-11-19
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2573196-8
ISSN	1792-1082 ; 1792-1074
ISSN (online)	1792-1082
ISSN	1792-1074
DOI	10.3892/ol.2014.2711
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In stock of ZB MED Cologne/Königswinter

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