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Article ; Online: Analysis of energy loss in the helical hedge flow channel of fruit tree root emitter

Jun Zhang / Xu Li / Shouping Zhang / Mengli Zhang

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 12

Abstract: Abstract This paper proposes the design of a helical hedge flow channel with a high energy loss, which shows promising potential for application in fruit tree root emitters. The aim is to investigate the relationship between the energy loss form in the ... ...

Abstract	Abstract This paper proposes the design of a helical hedge flow channel with a high energy loss, which shows promising potential for application in fruit tree root emitters. The aim is to investigate the relationship between the energy loss form in the channel and its influencing factors. The hydraulic performance testing method is employed to analyze the factors that affect energy loss. The main influencing factors are determined using the response surface methodology (RSM) for experimental design. Based on the obtained experimental results, the energy loss form and influencing factors are analyzed, and a prediction model for the energy loss coefficient (ξ) is established. The results indicate that the ξ exhibits a decreasing trend with an increase in the diversion angle (α), a trend of first increasing and then decreasing with an increase in the channel width (b), and an increasing trend with an increase in the number of channel units (n). The effects of the straight section length (l 1), convergence section length (l 2), and bend radius (r) on the ξ can be neglected. The ranking of the geometric parameters' influence on the ξ is as follows: n > b > α > l 1 > r > l 2. The experimental results reveal that the ξ ranges from 19.2 to 234.3. Furthermore, the head loss along the flow channel constitutes merely 0.06–0.47% of the local head loss, The main form of energy loss in the spiral counter flow channel is local head loss. There is a significant linear relationship between α, b, n and the ξ, The established prediction model (R2 = 0.9691) can accurately predict the ξ of the channel.
Keywords	Medicine ; R ; Science ; Q
Subject code	600
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Associations Between Forkhead Box L2 Expression and Ovary Development in Laying Hens

Shouping ZHANG / Xiaojing XIA / Lirong WANG / Renfeng LI / Meng YANG / Sanhu WANG

Kafkas Universitesi Veteriner Fakultesi Dergisi, Vol 25, Iss 3, Pp 305-

2019 Volume 309

Abstract: Make sure healthy ovary or follicle is critical for extending egg laying performance in poultry. Transcription factor forkhead box L2 (FOXL2) gene have key role in regulate development of ovary. In the present research, different aged Hy-line Brown hens ... ...

Abstract	Make sure healthy ovary or follicle is critical for extending egg laying performance in poultry. Transcription factor forkhead box L2 (FOXL2) gene have key role in regulate development of ovary. In the present research, different aged Hy-line Brown hens were maintained to explore relationships between ovarian developing and FOXL2 expression. Through histological observation, different quantities of follicles from various phases of age were observed. It was displayed that FOXL2 expression and number mature follicle were increased as the days of age increased and then decreased. In comparison, the expression of FOXL2 in hypothalamus and eyelid were remained in a relative stable level. Taken together, these data in our research establish a framework for understanding the potential functions of FOXL2 in regulate chicken ovarian developing and may provide a new perspective on the theory and practice to increase egg production or others.
Keywords	foxl2 ; follicle ; ovary ; laying ; poultry ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2019-05-01T00:00:00Z
Publisher	University of Kafkas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: DNAzymes Dz13 target the c-jun possess antiviral activity against influenza A viruses

Zhang, Zhaopei / Sanhu Wang / Shouping Zhang

Microbial pathogenesis. 2017 Feb., v. 103

2017

Abstract: The emergence of anti-influenza A virus drugs resistant strain highlights the need for more effective therapy. Our earlier study demonstrated that c-jun, a downstream molecule of JNK, might be important in viral infections and inflammatory responses. In ... ...

Abstract	The emergence of anti-influenza A virus drugs resistant strain highlights the need for more effective therapy. Our earlier study demonstrated that c-jun, a downstream molecule of JNK, might be important in viral infections and inflammatory responses. In the present study, we explored the function of DNAzymes Dz13 that target c-jun in influenza A virus infected mice. Dz13 displayed non-toxic side effects on A549 cells and BALB/c mice. Moreover, Dz13-treated mice had enhanced survival after influenza compared with untreated mice. Simultaneously, the pulmonary inflammatory responses and viral burden were decreased in Dz13 treated mice. Furthermore, proliferation levels of infection-induced CD4+ and CD8+ T cells were impaired. These data demonstrated that Dz13 could reduce viral replication and inflammatory response in vivo, suggesting that Dz13 may potentially be used to treat influenza A viral infection.
Keywords	adverse effects ; antiviral properties ; CD8-positive T-lymphocytes ; inflammation ; influenza ; Influenza A virus ; mice ; mitogen-activated protein kinase ; therapeutics ; viral load ; virus replication ; viruses
Language	English
Dates of publication	2017-02
Size	p. 155-161.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2016.12.024
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Development of a SYBR Green Real-Time PCR Assay with Melting Curve Analysis for Simultaneous Detection of Actinobacillus pleuropneumoniae and Haemophilus parasuis

Bin HU / Shouping ZHANG / Yanhua XU / Zhichen WANG / Qiuxuan REN / Jingfei XU / Yongjun DONG / Lirong WANG

Kafkas Universitesi Veteriner Fakultesi Dergisi, Vol 26, Iss 5, Pp 665-

2020 Volume 670

Abstract: In the present study, a duplex SYBR Green real-time PCR assay was developed in order to indentify Actinobacillus pleuropneumoniae and Haemophilus parasuis infection in one reaction, through a melting curve analysis. This method utilized two pairs of ... ...

Abstract	In the present study, a duplex SYBR Green real-time PCR assay was developed in order to indentify Actinobacillus pleuropneumoniae and Haemophilus parasuis infection in one reaction, through a melting curve analysis. This method utilized two pairs of specific primers that allowed the amplification of highly conserved regions of A. pleuropneumoniae Apx IV and H. parasuis omp P2 gene. Reconstitution experiments were conducted by using PMD - 19T plasmid in order to determine the sensitivity of the assay. The results showed that the Tm values of the melting curves of A. pleuropneumoniae and H. parasuis were 83.36±0.09ºC and 76.48±0.17ºC, respectively that could accurately distinguish these two pathogens. And no cross reaction were observed between other respiratory pathogens, which suggested a high specificity of two primers. The detection sensitivity of the assay was 127 and 96 copies/μL which was higher than that of the ordinary PCR detection methods. This rapid technique may present a simple, useful option for simultaneous detection of A. pleuropneumoniae and H. parasuis, which would be feasible and attractive for clinical samples diagnosis and epidemiological investigations.
Keywords	actinobacillus pleuropneumoniae ; haemophilus parasuis ; sybr green ι ; real-time pcr ; melting curve ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	University of Kafkas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The role of natural antimicrobial peptides during infection and chronic inflammation

Xia, Xiaojing / Likun Cheng / Shouping Zhang / Lei Wang / Jianhe Hu

Antonie van Leeuwenhoek. 2018 Jan., v. 111, no. 1

2018

Abstract: Natural antimicrobial peptides (AMPs), a family of small polypeptides that are produced by constitutive or inducible expression in organisms, are integral components of the host innate immune system. In addition to their broad-spectrum antibacterial ... ...

Abstract	Natural antimicrobial peptides (AMPs), a family of small polypeptides that are produced by constitutive or inducible expression in organisms, are integral components of the host innate immune system. In addition to their broad-spectrum antibacterial activity, natural AMPs also have many biological activities against fungi, viruses and parasites. Natural AMPs exert multiple immunomodulatory roles that may predominate under physiological conditions where they lose their microbicidal properties in serum and tissue environments. Increased drug resistance among microorganisms is occurring far more quickly than the discovery of new antibiotics. Natural AMPs have shown promise as ‘next generation antibiotics’ due to their broad-spectrum curative effects, low toxicity, the fact that they are not residual in animals, and the low rates of resistance exhibited by many pathogens. Many types of synthetic AMPs are currently being tested in clinical trials for the prevention and treatment of various diseases such as chemotherapy-associated infections, diabetic foot ulcers, catheter-related infections, and other conditions. Here, we provide an overview of the types and functions of natural AMPs and their role in combating microorganisms and different infectious and inflammatory diseases.
Keywords	animals ; antibacterial properties ; antibiotics ; antimicrobial peptides ; blood serum ; clinical trials ; diabetic foot ; drug resistance ; fungi ; inflammation ; innate immunity ; parasites ; pathogens ; polypeptides ; toxicity ; viruses
Language	English
Dates of publication	2018-01
Size	p. 5-26.
Publishing place	Springer International Publishing
Document type	Article
Note	Review
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-017-0929-0
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice

Chunling Zhu / Yaya Zhao / Xueqin Zhao / Shanqin Liu / Xiaojing Xia / Shouping Zhang / Yimin Wang / Huihui Zhang / Yanzhao Xu / Shijun Chen / Jinqing Jiang / Yundi Wu / Xilong Wu / Gaiping Zhang / Yueyu Bai / Jianhe Hu / Hanna Fotina / Lei Wang / Xueming Zhang

Frontiers in Veterinary Science, Vol

2022 Volume 9

Abstract: Staphylococcus aureus is a common pathogen that can cause pneumonia and a variety of skin diseases. Skin injuries have a high risk of colonization by S. aureus, which increases morbidity and mortality. Due to the emergence of multidrug-resistant strains, ...

Abstract	Staphylococcus aureus is a common pathogen that can cause pneumonia and a variety of skin diseases. Skin injuries have a high risk of colonization by S. aureus, which increases morbidity and mortality. Due to the emergence of multidrug-resistant strains, antimicrobial peptides are considered to be among the best alternatives to antibiotics due to their unique mechanism of action and other characteristics. MPX is an antibacterial peptide extracted from wasp venom that has antibacterial activity against a variety of bacteria. This study revealed that MPX has good bactericidal activity against S. aureus and that its minimum inhibitory concentration (MIC) is 0.08 μM. MPX (4×MIC) can kill 99.9% of bacteria within 1 h, and MPX has good stability. The research on the bactericidal mechanism found that MPX could destroy the membrane integrity, increase the membrane permeability, change the membrane electromotive force, and cause cellular content leakage, resulting in bactericidal activity. Results from a mouse scratch model experiment results show that MPX can inhibit colonization by S. aureus, which reduces the wound size, decreases inflammation, and promotes wound healing. This study reports the activity of MPX against S. aureus and its mechanism and reveals the ability of MPX to treat S. aureus infection in mice, laying the foundation for the development of new drugs for bacterial infections.
Keywords	antimicrobial peptide MPX ; Staphylococcus aureus ; membrane destruction ; wound healing ; inflammation ; Veterinary medicine ; SF600-1100
Subject code	571
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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Article ; Online: HbF Levels in Sickle Cell Disease Are Associated with Proportion of Circulating Hematopoietic Stem and Progenitor Cells and CC-Chemokines

Caterina P. Minniti / Seda S. Tolu / Kai Wang / Zi Yan / Karl Robert / Shouping Zhang / Andrew S. Crouch / Joan Uehlinger / Deepa Manwani / Eric E. Bouhassira

Cells, Vol 9, Iss 2199, p

2020 Volume 2199

Abstract: The concentration of circulating hematopoietic stem and progenitor cells has not been studied longitudinally. Here, we report that the proportions of Lin-CD34+38- hematopoietic multipotent cells (HMCs) and of Lin-CD34+CD38+ hematopoietic progenitors ... ...

Abstract	The concentration of circulating hematopoietic stem and progenitor cells has not been studied longitudinally. Here, we report that the proportions of Lin-CD34+38- hematopoietic multipotent cells (HMCs) and of Lin-CD34+CD38+ hematopoietic progenitors cells (HPCs) are highly variable between individuals but stable over long periods of time, in both healthy individuals and sickle cell disease (SCD) patients. This suggests that these proportions are regulated by genetic polymorphisms or by epigenetic mechanisms. We also report that in SCD patients treated with hydroxyurea, the proportions of circulating HMCs and HPCs show a strong positive and negative correlation with fetal hemoglobin (HbF) levels, respectively. Titration of 65 cytokines revealed that the plasma concentration of chemokines CCL2, CCL11, CCL17, CCL24, CCL27, and PDGF-BB were highly correlated with the proportion of HMCs and HPCs and that a subset of these cytokines were also correlated with HbF levels. A linear model based on four of these chemokines could explain 80% of the variability in the proportion of circulating HMCs between individuals. The proportion of circulating HMCs and HPCs and the concentration of these chemokines might therefore become useful biomarkers for HbF response to HU in SCD patients. Such markers might become increasingly clinically relevant, as alternative treatment modalities for SCD are becoming available.
Keywords	sickle cell disease ; hematopoietic stem and progenitor ; chemokine ; fetal hemoglobin ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Development of PPA-ELISA for Diagnosing Streptococcus suis Infection Using Recombinant Sao-M Protein As Diagnostic Antigen

Xiaojing XIA / Huihui ZHANG / Likun CHENG / Shouping ZHANG / Lei WANG / Shuguang LI / Zhiqiang SHEN / Gaiping ZHANG / Jianhe HU

Kafkas Universitesi Veteriner Fakultesi Dergisi, Vol 23, Iss 6, Pp 989-

2017 Volume 996

Abstract: Streptococcus suis, an important zoonotic agent, is responsible for outbreaks of human infections. The accurate and rapid detection of S. suis may help control the potential outbreak and ameliorate patient outcomes. In the present study, Sao-M was used ... ...

Abstract	Streptococcus suis, an important zoonotic agent, is responsible for outbreaks of human infections. The accurate and rapid detection of S. suis may help control the potential outbreak and ameliorate patient outcomes. In the present study, Sao-M was used to establish a horseradish peroxidase enzyme-linked staphylococcal protein A immunosorbent assay (PPA-ELISA) for the diagnosis of S. suis infection. Results of chessboard titration test showed that the optimal concentration of coating antigen and dilution of serum were 8 µg/ml and 1:80, respectively. The cut-off was confirmed as OD450≥0.351 for positive response. The specificity of test indicated that rSao-M had no cross-reaction with antisera against the other 6 species of pathogens. The variation coefficient of intra-batch and inter-batch in the repeating tests was less than 9.5%. Comparative analysis by using conventional ELISA kit and established GDH-based ELISA showed that the present PPA-ELISA has higher specificity and sensitivity than GDH-based ELISA. A total seroprevalence of 6.6% in 500 pig serum samples indicated the method’s applicability to detect S. suis infection. Cumulatively, the results suggested that the PPA-ELISA is a rapid, sensitive and specific diagnostic method and could be used as a new tool for large-scale epidemiological surveys and serological diagnosis of S. suis infection.
Keywords	streptococcus suis ; ppa-elisa ; sao-m ; diagnosis ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2017-11-01T00:00:00Z
Publisher	University of Kafkas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Transcription profiles of the responses of chicken bursae of Fabricius to IBDV in different timing phases

Ou, Changbo / Qiuxia Wang / Yanhong Zhang / Weili Kong / Shouping Zhang / Yan Yu / Jinyou Ma / Xingyou Liu / Xianghui Kong

Virology journal. 2017 Dec., v. 14, no. 1

2017

Abstract: BACKGROUND: Infectious bursal disease virus (IBDV) infection causes immunosuppression in chickens and increases their susceptibility to secondary infections. To explore the interaction between host and IBDV, RNA-Seq was applied to analyse the ... ...

Abstract	BACKGROUND: Infectious bursal disease virus (IBDV) infection causes immunosuppression in chickens and increases their susceptibility to secondary infections. To explore the interaction between host and IBDV, RNA-Seq was applied to analyse the transcriptional profiles of the responses of chickens’ bursas of Fabricius in the early stage of IBDV infection. RESULTS: The results displayed that a total of 15546 genes were identified in the chicken bursa libraries. Among the annotated genes, there were 2006 and 4668 differentially expressed genes in the infection group compared with the mock group on day 1 and day 3 post inoculation (1 and 3 dpi), respectively. Moreover, there were 676 common up-regulated and 83 common down-regulated genes in the bursae taken from the chickens infected with IBDV on both 1 and 3 dpi. Meanwhile, there were also some characteristic differentially expressed genes on 1 and 3 dpi. On day 1 after inoculation with IBDV, host responses mainly displayed immune response processes, while metabolic pathways played an important role on day three post infection. Six genes were confirmed by quantitative reverse transcription-PCR. CONCLUSIONS: In conclusion, the differential gene expression profile demonstrated with RNA-Seq might offer a better understanding of the molecular interactions between host and IBDV during the early stage of infection.
Keywords	Infectious bursal disease virus ; biochemical pathways ; bursa of Fabricius ; chickens ; gene expression regulation ; genes ; immune response ; immunosuppression ; reverse transcriptase polymerase chain reaction ; secondary infection ; sequence analysis ; transcription (genetics)
Language	English
Dates of publication	2017-12
Size	p. 93.
Publishing place	BioMed Central
Document type	Article
ISSN	1743-422X
DOI	10.1186/s12985-017-0757-x
Database	NAL-Catalogue (AGRICOLA)

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Article: Mice with type 1 diabetes exhibit increased susceptibility to influenza A virus

Huo, Caiyun / Hong Dong / Jin Xiao / Ming Wang / Peng Qi / Shouping Zhang / Siyi Zhang / Yanxin Hu

Microbial pathogenesis. 2017 Dec., v. 113

2017

Abstract: Type 1 diabetes (T1D) is a metabolic disease induced by abnormal insulin secretions from damaged islet B cells. Clinical observations have shown that T1D patients are more easily infected by influenza A virus (IAV) and suffer more serious symptoms than ... ...

Abstract	Type 1 diabetes (T1D) is a metabolic disease induced by abnormal insulin secretions from damaged islet B cells. Clinical observations have shown that T1D patients are more easily infected by influenza A virus (IAV) and suffer more serious symptoms than non-T1D patients. To investigate the susceptibility of T1D mice to IAV, a T1D mouse model was built by intraperitoneal injection of diluted streptozotocin (STZ) over 5 consecutive days, followed by infection with three subtypes of IAV (H1N1/H5N1/H7N2). The T1D-infected mice showed more serious clinical symptoms and lower survival rates than the non-T1D infected mice. The hematoxylin and eosin (H&E) staining results revealed an increase in serious pathological damage to the lung and pancreas in T1D-infected mice. Immunohistochemistry results indicated higher IAV loads and a more extensive distribution of positive signals in the lungs and pancreas of T1D-infected mice than in those of non-T1D infected mice. Furthermore, according to real-time quantitative polymerase chain reaction (PCR) results, viral replication appeared to occur more easily in the lungs of T1D-infected mice. Thus, T1D-infected mice exhibited higher susceptibility to IAV than did normal mice. This study contributes a mouse model suitable for T1D research as well as valuable information about the mechanism underlying T1D patients' increased susceptibility to IAV.
Keywords	animal models ; B-lymphocytes ; immunohistochemistry ; Influenza A virus ; insulin-dependent diabetes mellitus ; intraperitoneal injection ; lungs ; mice ; pancreas ; patients ; polymerase chain reaction ; staining ; streptozotocin ; survival rate ; virus replication
Language	English
Dates of publication	2017-12
Size	p. 233-241.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2017.10.026
Database	NAL-Catalogue (AGRICOLA)

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