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  1. Article ; Online: Metabolic and Oxidative Stress Effects on the Spectroelectrochemical Behavior of Single

    Cutri, Allison R / Shrout, Joshua D / Bohn, Paul W

    Chemical & biomedical imaging

    2023  Volume 1, Issue 7, Page(s) 659–666

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2023-10-12
    Publishing country United States
    Document type Journal Article
    ISSN 2832-3637
    ISSN (online) 2832-3637
    DOI 10.1021/cbmi.3c00083
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  2. Article ; Online: Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.

    Parmar, Dharmeshkumar / Rosado-Rosa, Joenisse M / Shrout, Joshua D / Sweedler, Jonathan V

    Methods (San Diego, Calif.)

    2024  Volume 224, Page(s) 21–34

    Abstract: Biofilms are dense aggregates of bacterial colonies embedded inside a self-produced polymeric matrix. Biofilms have received increasing attention in medical, industrial, and environmental settings due to their enhanced survival. Their characterization ... ...

    Abstract Biofilms are dense aggregates of bacterial colonies embedded inside a self-produced polymeric matrix. Biofilms have received increasing attention in medical, industrial, and environmental settings due to their enhanced survival. Their characterization using microscopy techniques has revealed the presence of structural and cellular heterogeneity in many bacterial systems. However, these techniques provide limited chemical detail and lack information about the molecules important for bacterial communication and virulence. Mass spectrometry imaging (MSI) bridges the gap by generating spatial chemical information with unmatched chemical detail, making it an irreplaceable analytical platform in the multi-modal imaging of biofilms. In the last two decades, over 30 species of biofilm-forming bacteria have been studied using MSI in different environments. The literature conveys both analytical advancements and an improved understanding of the effects of environmental variables such as host surface characteristics, antibiotics, and other species of microorganisms on biofilms. This review summarizes the insights from frequently studied model microorganisms. We share a detailed list of organism-wide metabolites, commonly observed mass spectral adducts, culture conditions, strains of bacteria, substrate, broad problem definition, and details of the MS instrumentation, such as ionization sources and matrix, to facilitate future studies. We also compared the spatial characteristics of the secretome under different study designs to highlight changes because of various environmental influences. In addition, we highlight the current limitations of MSI in relation to biofilm characterization to enable cross-comparison between experiments. Overall, MSI has emerged to become an important approach for the spatial/chemical characterization of bacterial biofilms and its use will continue to grow as MSI becomes more accessible.
    MeSH term(s) Mass Spectrometry ; Biofilms ; Bacteria/genetics ; Diagnostic Imaging
    Language English
    Publishing date 2024-01-29
    Publishing country United States
    Document type Review ; Journal Article
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2024.01.014
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3239: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Spectroelectrochemical behavior of parallel arrays of single vertically oriented Pseudomonas aeruginosa cells.

    Cutri, Allison R / Sundaresan, Vignesh / Shrout, Joshua D / Bohn, Paul W

    Cell reports. Physical science

    2023  Volume 4, Issue 4

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2023-04-06
    Publishing country United States
    Document type Journal Article
    ISSN 2666-3864
    ISSN (online) 2666-3864
    DOI 10.1016/j.xcrp.2023.101368
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  4. Article ; Online: A fantastic voyage for sliding bacteria.

    Shrout, Joshua D

    Trends in microbiology

    2015  Volume 23, Issue 5, Page(s) 244–246

    Abstract: A recent study showed that Salmonella enterica serovar Typhimurium exhibits sliding motility under magnesium-limited conditions. Overall, bacteria that exhibit this passive surface movement described as sliding share few common traits. This discovery ... ...

    Abstract A recent study showed that Salmonella enterica serovar Typhimurium exhibits sliding motility under magnesium-limited conditions. Overall, bacteria that exhibit this passive surface movement described as sliding share few common traits. This discovery provides an opportunity to revisit and better characterize appendage-independent bacterial motility.
    MeSH term(s) Bacterial Physiological Phenomena ; Bacterial Proteins/genetics ; Bacterial Proteins/physiology ; Flagella/physiology ; Genes, Bacterial ; Movement ; Salmonella typhimurium/genetics ; Salmonella typhimurium/physiology
    Chemical Substances Bacterial Proteins
    Language English
    Publishing date 2015-05
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2015.03.001
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3738: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Alkyl quinolones mediate heterogeneous colony biofilm architecture that improves community-level survival.

    Weaver, Abigail A / Jia, Jin / Cutri, Allison R / Madukoma, Chinedu S / Vaerewyck, Catherine M / Bohn, Paul W / Shrout, Joshua D

    Journal of bacteriology

    2024  Volume 206, Issue 4, Page(s) e0009524

    Abstract: Bacterial communities exhibit complex self-organization that contributes to their survival. To better understand the molecules that contribute to transforming a small number of cells into a heterogeneous surface biofilm community, we studied acellular ... ...

    Abstract Bacterial communities exhibit complex self-organization that contributes to their survival. To better understand the molecules that contribute to transforming a small number of cells into a heterogeneous surface biofilm community, we studied acellular aggregates, structures seen by light microscopy in
    MeSH term(s) Humans ; Quinolones/metabolism ; Biofilms ; Pseudomonas Infections/microbiology ; Virulence ; Pseudomonas aeruginosa/metabolism
    Chemical Substances Quinolones
    Language English
    Publishing date 2024-04-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00095-24
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Differential Spreading of Rhamnolipid Congeners from

    Weaver, Abigail A / Parmar, Dharmeshkumar / Junker, Ella A / Sweedler, Jonathan V / Shrout, Joshua D

    ACS applied bio materials

    2023  Volume 6, Issue 11, Page(s) 4914–4921

    Abstract: Rhamnolipids are surfactants produced by many Pseudomonad bacteria, including the ... ...

    Abstract Rhamnolipids are surfactants produced by many Pseudomonad bacteria, including the species
    MeSH term(s) Pseudomonas aeruginosa ; Glycolipids/chemistry ; Biofilms ; Bacteria ; Surface-Active Agents/chemistry
    Chemical Substances rhamnolipid ; Glycolipids ; Surface-Active Agents
    Language English
    Publishing date 2023-10-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.3c00641
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  7. Article ; Online: The 24th Annual Midwest Microbial Pathogenesis Meeting.

    Champion, Patricia A / Shrout, Joshua D

    Journal of bacteriology

    2018  Volume 200, Issue 11, Page(s) e000950–18

    Abstract: The 24th Annual Midwest Microbial Pathogenesis Conference (MMPC) was held at the University of Notre Dame from August 25-27, 2017. The conference provided an opportunity for scientists from the Midwest to discuss new advances in microbial pathogenesis, ... ...

    Abstract The 24th Annual Midwest Microbial Pathogenesis Conference (MMPC) was held at the University of Notre Dame from August 25-27, 2017. The conference provided an opportunity for scientists from the Midwest to discuss new advances in microbial pathogenesis, including how pathogens promote disease, and how they interact with each other, the microbiome and the host. This commentary highlights the MMPC history, the topics presented at the conference and the reports in this issue.
    Language English
    Publishing date 2018-02-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.00095-18
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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  8. Article ; Online: Electrochemical and spectroelectrochemical characterization of bacteria and bacterial systems.

    Sundaresan, Vignesh / Do, Hyein / Shrout, Joshua D / Bohn, Paul W

    The Analyst

    2021  Volume 147, Issue 1, Page(s) 22–34

    Abstract: Microbes, such as bacteria, can be described, at one level, as small, self-sustaining chemical factories. Based on the species, strain, and even the environment, bacteria can be useful, neutral or pathogenic to human life, so it is increasingly important ...

    Abstract Microbes, such as bacteria, can be described, at one level, as small, self-sustaining chemical factories. Based on the species, strain, and even the environment, bacteria can be useful, neutral or pathogenic to human life, so it is increasingly important that we be able to characterize them at the molecular level with chemical specificity and spatial and temporal resolution in order to understand their behavior. Bacterial metabolism involves a large number of internal and external electron transfer processes, so it is logical that electrochemical techniques have been employed to investigate these bacterial metabolites. In this mini-review, we focus on electrochemical and spectroelectrochemical methods that have been developed and used specifically to chemically characterize bacteria and their behavior. First, we discuss the latest mechanistic insights and current understanding of microbial electron transfer, including both direct and mediated electron transfer. Second, we summarize progress on approaches to spatiotemporal characterization of secreted factors, including both metabolites and signaling molecules, which can be used to discern how natural or external factors can alter metabolic states of bacterial cells and change either their individual or collective behavior. Finally, we address
    MeSH term(s) Bacteria/genetics ; Humans ; Mass Gatherings
    Language English
    Publishing date 2021-12-20
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d1an01954f
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    Zs.A 2423: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article: Spatiotemporal distribution of chemical signatures exhibited by Myxococcus xanthus in response to metabolic conditions

    Do, Hyein / Madukoma, Chinedu S. / Sundaresan, Vignesh / Shrout, Joshua D. / Hoffman, Anthony J. / Bohn, Paul W.

    Analytical and bioanalytical chemistry. 2022 Feb., v. 414, no. 4

    2022  

    Abstract: Myxococcus xanthus is a common soil bacterium with a complex life cycle, which is known for production of secondary metabolites. However, little is known about the effects of nutrient availability on M. xanthus metabolite production. In this study, we ... ...

    Abstract Myxococcus xanthus is a common soil bacterium with a complex life cycle, which is known for production of secondary metabolites. However, little is known about the effects of nutrient availability on M. xanthus metabolite production. In this study, we utilize confocal Raman microscopy (CRM) to examine the spatiotemporal distribution of chemical signatures secreted by M. xanthus and their response to varied nutrient availability. Ten distinct spectral features are observed by CRM from M. xanthus grown on nutrient-rich medium. However, when M. xanthus is constrained to grow under nutrient-limited conditions, by starving it of casitone, it develops fruiting bodies, and the accompanying Raman microspectra are dramatically altered. The reduced metabolic state engendered by the absence of casitone in the medium is associated with reduced, or completely eliminated, features at 1140 cm⁻¹, 1560 cm⁻¹, and 1648 cm⁻¹. In their place, a feature at 1537 cm⁻¹ is observed, this feature being tentatively assigned to a transitional phase important for cellular adaptation to varying environmental conditions. In addition, correlating principal component analysis heat maps with optical images illustrates how fruiting bodies in the center co-exist with motile cells at the colony edge. While the metabolites responsible for these Raman features are not completely identified, three M. xanthus peaks at 1004, 1151, and 1510 cm⁻¹ are consistent with the production of lycopene. Thus, a combination of CRM imaging and PCA enables the spatial mapping of spectral signatures of secreted factors from M. xanthus and their correlation with metabolic conditions.
    Keywords Myxococcus xanthus ; Raman imaging ; analytical chemistry ; heat ; lycopene ; nutrient availability ; principal component analysis ; secondary metabolites ; soil bacteria
    Language English
    Dates of publication 2022-02
    Size p. 1691-1698.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ISSN 1618-2642
    DOI 10.1007/s00216-021-03795-6
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  10. Article: A fantastic voyage for sliding bacteria

    Shrout, Joshua D

    Trends in microbiology. 2015 May, v. 23, no. 5

    2015  

    Abstract: A recent study showed that Salmonella enterica serovar Typhimurium exhibits sliding motility under magnesium-limited conditions. Overall, bacteria that exhibit this passive surface movement described as sliding share few common traits. This discovery ... ...

    Abstract A recent study showed that Salmonella enterica serovar Typhimurium exhibits sliding motility under magnesium-limited conditions. Overall, bacteria that exhibit this passive surface movement described as sliding share few common traits. This discovery provides an opportunity to revisit and better characterize appendage-independent bacterial motility.
    Keywords bacteria ; bacterial motility ; Salmonella Typhimurium
    Language English
    Dates of publication 2015-05
    Size p. 244-246.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2015.03.001
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    In stock of ZB MED Bonn / Germany

    Z 2005/714: Show issues

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