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Article ; Online: Serum Pentaxin 3 (PTX3) Promotes NLRP3 Inflammasome and Pyroptosis in Patients with Up-Regulated Myasthenia Gravis

Yao Li / Shuhui Wang / Yunpeng Liu / Yongbo Zhang

Frontiers in Bioscience-Landmark, Vol 28, Iss 11, p

2023 Volume 306

Abstract: Background: Myasthenia gravis (MG) is an autoantibodies-mediated autoimmune disease with the complications of neuromuscular junction transmission. In this study, we aimed to investigate the molecular regulatory roles of pentaxin 3 (PTX3) in patients and ... ...

Abstract	Background: Myasthenia gravis (MG) is an autoantibodies-mediated autoimmune disease with the complications of neuromuscular junction transmission. In this study, we aimed to investigate the molecular regulatory roles of pentaxin 3 (PTX3) in patients and in animal model with MG and to explore its underlying mechanism. Methods: Patients with MG were identified and enrolled at our designated hospital and animal model was utilized for the proposed study. Enzyme-linked immunosorbent assay (ELISA) kit were used to quantify the IL-1β, IL-6, INF-γ, IL-17, TNF-α, anti-TAChR IgG/IgG1/IgG2b/IgG2c levels. Results: Serum PTX3 expression level in patients with MG was up-regulated as compared to normal. Furthermore, we found increased expression level of mRNA and protein product of PTX3 in the mice with MG. PTX3 promoted inflammation, pyroptosis in patients as well as in the MG mouse model. In addition, PTX3 induced the STAT3/NLRP3 inflammasome and promoted gene synthesis of STAT3. We found that METTL3-mediated m6A modification decreases PTX3 stability. Conclusions: Our study suggests that the PTX3 is associated with the enhancement of inflammation and pyroptosis through regulating the STAT3/NLRP3 inflammasome signaling pathway at the early stage of the disease. The pro-inflammatory PTX3 facilitates the development of MG and it can be used as a potantial MG-associated diagnostic biomarker for MG.
Keywords	myasthenia gravis ; nlrp3 ; inflammasome ; ptx3 ; neuromuscular disease ; Biochemistry ; QD415-436 ; Biology (General) ; QH301-705.5
Subject code	610 ; 616
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	IMR Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: HDL Is Not Dead Yet.

Lorkowski, Shuhui Wang / Smith, Jonathan D

Biomedicines

2022 Volume 10, Issue 1

Abstract: High-density lipoprotein cholesterol (HDL-C) levels are inversely correlated with coronary heart disease (CHD) in multiple epidemiological studies, but whether HDL is causal or merely associated with CHD is unclear. Recent trials for HDL-raising drugs ... ...

Abstract	High-density lipoprotein cholesterol (HDL-C) levels are inversely correlated with coronary heart disease (CHD) in multiple epidemiological studies, but whether HDL is causal or merely associated with CHD is unclear. Recent trials for HDL-raising drugs were either not effective in reducing CHD events or, if beneficial in reducing CHD events, were not conclusive as the findings could be attributed to the drugs' LDL-reducing activity. Furthermore, the first large Mendelian randomization study did not causally relate HDL-C levels to decreased CHD. Thus, the hypothesis that HDL is protective against CHD has been rightfully challenged. However, subsequent Mendelian randomization studies found HDL characteristics that are causally related to decreased CHD. Many aspects of HDL structure and function, especially in reverse cholesterol transport, may be better indicators of HDL's protective activity than simply measuring HDL-C. Cholesterol efflux capacity is associated with lower levels of prevalent and incident CHD, even after adjustment for HDL-C and apolipoprotein A-1 levels. Also, subjects with very high levels of HDL-C, including those with rare mutations that disrupt hepatic HDL uptake and reverse cholesterol transport, may be at higher risk for CHD than those with moderate levels. We describe here several cell-based and cell-free in vitro assays of HDL structure and function that may be used in clinical studies to determine which of HDL's functions are best associated with protection against CHD. We conclude that the HDL hypothesis may need revision based on studies of HDL structure and function, but that the HDL hypothesis is not dead yet.
Language	English
Publishing date	2022-01-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10010128
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Article: Dual-Proxy Modeling for Masked Face Recognition.

Shuhui, Wang / Xiaochen, Mao

Procedia computer science

2022 Volume 208, Page(s) 145–151

Abstract: With the recent worldwide COVID-19 pandemic, almost everyone wears a mask daily, leading to severe degradation in the accuracy of conventional face recognition systems. Several works improve the performance of masked faces by adopting synthetic masked ... ...

Abstract	With the recent worldwide COVID-19 pandemic, almost everyone wears a mask daily, leading to severe degradation in the accuracy of conventional face recognition systems. Several works improve the performance of masked faces by adopting synthetic masked face images for training. However, such methods often cause performance degradation on unmasked faces, raising the contradiction between the face recognition system's accuracy on unmasked and masked faces. In this paper, we propose a dual-proxy face recognition training method to improve masked faces' performance while maintaining unmasked faces' performance. Specifically, we design two fully-connected layers as the unmasked and masked feature space proxies to alleviate the significant difference between the two data distributions. The cross-space constraints are adopted to ensure the intra-class compactness and inter-class discrepancy. Extensive experiments on popular unmasked face benchmarks and masked face benchmarks, including real-world mask faces and the generated mask faces, demonstrate our method's superiority over the state-of-the-art methods on masked faces without incurring a notable accuracy degradation on unmasked faces.
Language	English
Publishing date	2022-11-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2557358-5
ISSN	1877-0509
ISSN	1877-0509
DOI	10.1016/j.procs.2022.10.022
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Article ; Online: The Characterization and Differential Analysis of m 6 A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat

Gang Luo / Yaotian Ai / Lin Yu / Shuhui Wang / Zhanjun Ren

Animals, Vol 13, Iss 446, p

2023 Volume 446

Abstract: N6-methyladenosine (m 6 A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m 6 A regulating intramuscular fat deposition in rabbits. In this study, m 6 A ... ...

Abstract	N6-methyladenosine (m 6 A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m 6 A regulating intramuscular fat deposition in rabbits. In this study, m 6 A modification of Hycole rabbit muscle and adipose tissues were detected by MeRIP-Seq. In this case, 3 methylases and 12 genes modified by m 6 A were found to be significantly different between muscle and adipose tissues. At the same time, we found 3 methylases can regulate the expression of 12 genes in different ways and the function of 12 genes is related to fat deposition base on existing studies. 12 genes were modified by m 6 A methylase in rabbit muscle and adipose tissues. These results suggest that 3 methylases may regulate the expression of 12 genes through different pathways. In addition, the analysis of results showed that 6 of the 12 genes regulated eight signaling pathways, which regulated intramuscular fat deposition. RT-qPCR was used to validate the sequencing results and found the expression results of RT-qPCR and sequencing results are consistent. In summary, METTL4 , ZC3H13 and IGF2BP2 regulated intramuscular fat by m 6 A modified gene/signaling pathways. Our work provided a new molecular basis and a new way to produce rabbit meat with good taste.
Keywords	Hycole rabbit ; m 6 A ; gene expression ; methylase ; intramuscula fat ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Subject code	333
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Correction to: The practical utility of AI-assisted molecular profiling in the diagnosis and management of cancer of unknown primary: An updated review.

Lorkowski, Shuhui Wang / Dermawan, Josephine K / Rubin, Brian P

Virchows Archiv : an international journal of pathology

2023 Volume 484, Issue 2, Page(s) 377

Language	English
Publishing date	2023-12-07
Publishing country	Germany
Document type	Published Erratum
ZDB-ID	1184867-4
ISSN	1432-2307 ; 0945-6317
ISSN (online)	1432-2307
ISSN	0945-6317
DOI	10.1007/s00428-023-03728-x
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Article ; Online: The practical utility of AI-assisted molecular profiling in the diagnosis and management of cancer of unknown primary: an updated review.

Lorkowski, Shuhui Wang / Dermawan, Josephine K / Rubin, Brian P

Virchows Archiv : an international journal of pathology

2023 Volume 484, Issue 2, Page(s) 369–375

Abstract: Cancer of unknown primary (CUP) presents a complex diagnostic challenge, characterized by metastatic tumors of unknown tissue origin and a dismal prognosis. This review delves into the emerging significance of artificial intelligence (AI) and machine ... ...

Abstract	Cancer of unknown primary (CUP) presents a complex diagnostic challenge, characterized by metastatic tumors of unknown tissue origin and a dismal prognosis. This review delves into the emerging significance of artificial intelligence (AI) and machine learning (ML) in transforming the landscape of CUP diagnosis, classification, and treatment. ML approaches, trained on extensive molecular profiling data, have shown promise in accurately predicting tissue of origin. Genomic profiling, encompassing driver mutations and copy number variations, plays a pivotal role in CUP diagnosis by providing insights into tumor type-specific oncogenic alterations. Mutational signatures (MS), reflecting somatic mutation patterns, offer further insights into CUP diagnosis. Known MS with established etiology, such as ultraviolet (UV) light-induced DNA damage and tobacco exposure, have been identified in cases of dedifferentiated/transdifferentiated melanoma and carcinoma. Deep learning models that integrate gene expression data and DNA methylation patterns offer insights into tissue lineage and tumor classification. In digital pathology, machine learning algorithms analyze whole-slide images to aid in CUP classification. Finally, precision oncology, guided by molecular profiling, offers targeted therapies independent of primary tissue identification. Clinical trials assigning CUP patients to molecularly guided therapies, including targetable alterations and tumor mutation burden as an immunotherapy biomarker, have resulted in improved overall survival in a subset of patients. In conclusion, AI- and ML-driven approaches are revolutionizing CUP management by enhancing diagnostic accuracy. Precision oncology utilizing enhanced molecular profiling facilitates the identification of targeted therapies that transcend the need to identify the tissue of origin, ultimately improving patient outcomes.
MeSH term(s)	Humans ; Neoplasms, Unknown Primary/diagnosis ; Neoplasms, Unknown Primary/genetics ; Neoplasms, Unknown Primary/therapy ; Gene Expression Profiling/methods ; Artificial Intelligence ; DNA Copy Number Variations ; Precision Medicine
Language	English
Publishing date	2023-11-24
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1184867-4
ISSN	1432-2307 ; 0945-6317
ISSN (online)	1432-2307
ISSN	0945-6317
DOI	10.1007/s00428-023-03708-1
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Article ; Online: HDL Is Not Dead Yet

Shuhui Wang Lorkowski / Jonathan D. Smith

Biomedicines, Vol 10, Iss 128, p

2022 Volume 128

Abstract	High-density lipoprotein cholesterol (HDL-C) levels are inversely correlated with coronary heart disease (CHD) in multiple epidemiological studies, but whether HDL is causal or merely associated with CHD is unclear. Recent trials for HDL-raising drugs were either not effective in reducing CHD events or, if beneficial in reducing CHD events, were not conclusive as the findings could be attributed to the drugs’ LDL-reducing activity. Furthermore, the first large Mendelian randomization study did not causally relate HDL-C levels to decreased CHD. Thus, the hypothesis that HDL is protective against CHD has been rightfully challenged. However, subsequent Mendelian randomization studies found HDL characteristics that are causally related to decreased CHD. Many aspects of HDL structure and function, especially in reverse cholesterol transport, may be better indicators of HDL’s protective activity than simply measuring HDL-C. Cholesterol efflux capacity is associated with lower levels of prevalent and incident CHD, even after adjustment for HDL-C and apolipoprotein A-1 levels. Also, subjects with very high levels of HDL-C, including those with rare mutations that disrupt hepatic HDL uptake and reverse cholesterol transport, may be at higher risk for CHD than those with moderate levels. We describe here several cell-based and cell-free in vitro assays of HDL structure and function that may be used in clinical studies to determine which of HDL’s functions are best associated with protection against CHD. We conclude that the HDL hypothesis may need revision based on studies of HDL structure and function, but that the HDL hypothesis is not dead yet.
Keywords	HDL function ; reverse cholesterol transport ; apoA1 exchange rate ; Mendelian randomization ; inflammation ; coronary heart disease ; Biology (General) ; QH301-705.5
Subject code	616
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Assessment of influencing factors on non-point source pollution critical source areas in an agricultural watershed

Shuhui Wang / Yunqi Wang / Yujie Wang / Zhen Wang

Ecological Indicators, Vol 141, Iss , Pp 109084- (2022)

2022

Abstract: Critical Source Areas (CSAs) are areas that contribute disproportionate high levels of non-point source (NPS) pollution to receiving waters, and their occurrence is the result of the complex interaction between the factors related to the sources and ... ...

Abstract	Critical Source Areas (CSAs) are areas that contribute disproportionate high levels of non-point source (NPS) pollution to receiving waters, and their occurrence is the result of the complex interaction between the factors related to the sources and transport processes of NPS pollution. A systematic understanding of how these influencing factors affect CSAs is essential for successful watershed management. In this study, we applied a statistical data mining technique boosted regression tree model to quantify the contribution of eight influencing factors (soil type, slope, elevation, RUSLE LS factor, RUSLE K factor, runoff, fertilizer application rate and land use) on two types of CSAs (TN-CSAs and TP-CSAs), as well as the marginal effects and potential thresholds of influencing factors on the occurrence of CSAs. Results show that land use (37.35%, 25.03%), fertilizer application (36.93%, 57.83%) and soil type (17.59%, 13.70%) have higher importance in determining the occurrence of TN-CSAs and TP-CSAs; and the incidence of TN-CSAs is positively correlated with most factors before the threshold for each influencing factor, after which the marginal effect largely leveled off or dropped slightly; TP-CSAs have essentially the same characteristics as TN-CSAs, but TP-CSAs are more likely to occur in areas with an annual runoff of around 244.92 mm. In addition, this study discussed the application of machine learning techniques in predicting CSAs under climate change without physical-based models, as well as a preliminary watershed management planning for NPS pollution control in the study watershed. These results provided important information for nutrient management regulations.
Keywords	Non-point source pollution ; Critical source areas ; AnnAGNPS model ; Boosted regression tree ; Watershed planning ; Ecology ; QH540-549.5
Subject code	333
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

Ruiguang Gong / Xiangyang Ye / Shuhui Wang / Zhanjun Ren

PLoS ONE, Vol 16, Iss

2021 Volume 11

Abstract: In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment ... ...

Abstract	In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm—2.5 mm, the diameter of the bacteria was 0.5 μm—1.0 μm × 2.0 μm—6.3 μm, and the spore diameter was 1 μm—1.2 μm × 1 μm—1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit.

Ruiguang Gong / Xiangyang Ye / Shuhui Wang / Zhanjun Ren

PLoS ONE, Vol 16, Iss 11, p e

2021 Volume 0259715

Abstract	In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm-2.5 mm, the diameter of the bacteria was 0.5 μm-1.0 μm × 2.0 μm-6.3 μm, and the spore diameter was 1 μm-1.2 μm × 1 μm-1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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