LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article: Biochemical parameters and 18S rRNA gene sequence analysis amongst green microalgal strains from selected aquatic sites of Eastern India.

    Vishwakarma, Rashi / Dhar, Dolly Wattal / Jena, Mrutyunjay / Shukla, Madhulika

    Water science and technology : a journal of the International Association on Water Pollution Research

    2020  Volume 82, Issue 6, Page(s) 1205–1216

    Abstract: In the present study, 24 green microalgae strains were isolated from selected aquatic sites of India. These were microscopically identified as Chlamydomonas sp., Scenedesmus sp., Chlorella sp., Dictyosphaerium sp. and Dunaliella sp. Nannochloropsis sp. ( ... ...

    Abstract In the present study, 24 green microalgae strains were isolated from selected aquatic sites of India. These were microscopically identified as Chlamydomonas sp., Scenedesmus sp., Chlorella sp., Dictyosphaerium sp. and Dunaliella sp. Nannochloropsis sp. (MCC 25), was used as a reference strain. Results showed that Dictyosphaerium sp. (MCC 10 and MCC 12) showed relatively higher nutritive content. The total soluble proteins in the reference strain was 21.4%, whereas it showed carbohydrate content of 17.2% and the lipids were 3.4% on a dry weight basis. Best performing strains were identified by biochemical characterization. Five genera were selected for molecular identification since they were the most representative based upon their area of isolation and their optimum content of total soluble proteins, carbohydrates and lipids. 18S rRNA sequencing authenticated their identification as Scenedesmus sp., Dictyosphaerium sp. and Chlorella sp. The sequences of these have been submitted in NCBI database with accession numbers as KT808247-KT808251. The correlation matrix showed positive correlation between carbohydrates and lipids, while negative correlation was seen between proteins and carbohydrates and between proteins and lipids. This study emphasizes the need for complete compositional analysis of the biomass for the possible applicability in the area of value addition.
    MeSH term(s) Chlorella/genetics ; Genes, rRNA ; India ; Microalgae/genetics ; RNA, Ribosomal, 18S/genetics ; Sequence Analysis
    Chemical Substances RNA, Ribosomal, 18S
    Language English
    Publishing date 2020-10-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 764273-8
    ISSN 1996-9732 ; 0273-1223
    ISSN (online) 1996-9732
    ISSN 0273-1223
    DOI 10.2166/wst.2020.195
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Einzelsignatur: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Vemurafenib and Obinutuzumab as Frontline Therapy for Hairy Cell Leukemia.

    Park, Jae H / Devlin, Sean / Durham, Benjamin H / Winer, Eric S / Huntington, Scott / von Keudell, Gottfried / Vemuri, Shreya / Shukla, Madhulika / Falco, Victoria / Cuello, Bernadette / Gore, Steven / Stone, Richard / Abdel-Wahab, Omar / Tallman, Martin S

    NEJM evidence

    2023  Volume 2, Issue 10, Page(s) EVIDoa2300074

    Abstract: Vemurafenib and Obinutuzumab for Hairy Cell LeukemiaIn this study of vemurafenib plus obinutuzumab of patients with previously untreated hairy cell leukemia, treatment was administered for four cycles, and the primary end point was complete remission ... ...

    Abstract Vemurafenib and Obinutuzumab for Hairy Cell LeukemiaIn this study of vemurafenib plus obinutuzumab of patients with previously untreated hairy cell leukemia, treatment was administered for four cycles, and the primary end point was complete remission rate. Twenty-seven of 30 patients completed all four cycles of treatment and achieved complete remission. No dose-limiting toxicity was observed.
    MeSH term(s) Humans ; Vemurafenib ; Leukemia, Hairy Cell/chemically induced ; Antibodies, Monoclonal, Humanized/adverse effects ; Remission Induction
    Chemical Substances Vemurafenib (207SMY3FQT) ; obinutuzumab (O43472U9X8) ; Antibodies, Monoclonal, Humanized
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Journal Article
    ISSN 2766-5526
    ISSN (online) 2766-5526
    DOI 10.1056/EVIDoa2300074
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Influence of light intensity, temperature and CO₂ concentration on growth and lipids in green algae and cyanobacteria.

    Shukla, Madhulika / Tabassum, Rizwana / Singh, Rajendra / Dhar, Dolly Wattal

    Indian journal of experimental biology

    2016  Volume 54, Issue 7, Page(s) 482–487

    Abstract: Effects of the environmental variables such as light intensity (Vmol photons m⁻² S⁻¹), temperature (*C) and CO₂ concentration (ppm) on chlorophyll, total soluble proteins and lipids were studied in selected microalgal strains from Chlorophyceae ( ... ...

    Abstract Effects of the environmental variables such as light intensity (Vmol photons m⁻² S⁻¹), temperature (*C) and CO₂ concentration (ppm) on chlorophyll, total soluble proteins and lipids were studied in selected microalgal strains from Chlorophyceae (Chlamydomonas sp., Scenedesmus sp., Chlorella sp., Kirchneriella sp.) and cyanobacteria (Nostoc sp.1, Anabaena sp., Nostoc sp. 2, Cylindrospermum sp.). Cultures were grown under controlled conditions at the National Phytotron Facility, Indian Agricultural Research Institute (IARI), New Delhi. Our results showed that chlorophyll concentration enhanced with increased C0₂. Chlorella exhibited the highest chlorophyll at 850 ppm CO₂ and 28*C; for Chlamydomonas it was at 78 μmol photons m⁻² S⁻¹ light intensity. In Cylindrospernum, total soluble proteins decreased with enhanced C0₂, and were highest at 18*C. In Anabaena, a light intensity of 65 μmol photons m⁻² S⁻¹ was best for maximum total soluble proteins. In Chlorella, CO₂ @ 850 ppm was most suited for maximum lipid accumulation. In Kirchneriella, increase in temperature, from 18*C up to 370C, increased total lipids; the highest was at 28'C. In Chlamydomonas, the light intensity of 78 μmol photons m⁻² S⁻¹ was optimum for lipid accumulation and the maximum total lipids was 30.8 (% dry wt.).
    MeSH term(s) Carbon Dioxide/pharmacology ; Chlorophyta/chemistry ; Chlorophyta/growth & development ; Chlorophyta/metabolism ; Cyanobacteria/chemistry ; Cyanobacteria/growth & development ; Cyanobacteria/metabolism ; Light ; Lipid Metabolism ; Lipids/analysis ; Temperature
    Chemical Substances Lipids ; Carbon Dioxide (142M471B3J)
    Language English
    Publishing date 2016-07
    Publishing country India
    Document type Journal Article
    ZDB-ID 416061-7
    ISSN 0975-1009 ; 0019-5189
    ISSN (online) 0975-1009
    ISSN 0019-5189
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 800: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Long-term outcomes in patients with relapsed or refractory hairy cell leukemia treated with vemurafenib monotherapy.

    Handa, Shivani / Lee, Jeong-Ok / Derkach, Andriy / Stone, Richard M / Saven, Alan / Altman, Jessica K / Grever, Michael R / Rai, Kanti R / Shukla, Madhulika / Vemuri, Shreya / Montoya, Skye / Taylor, Justin / Abdel-Wahab, Omar / Tallman, Martin S / Park, Jae H

    Blood

    2022  Volume 140, Issue 25, Page(s) 2663–2671

    Abstract: Vemurafenib, an oral BRAF inhibitor, has demonstrated high response rates in relapsed/refractory (R/R) hairy cell leukemia (HCL). However, little is known about long-term outcomes and response to retreatment. Herein, we report the results of 36 patients ... ...

    Abstract Vemurafenib, an oral BRAF inhibitor, has demonstrated high response rates in relapsed/refractory (R/R) hairy cell leukemia (HCL). However, little is known about long-term outcomes and response to retreatment. Herein, we report the results of 36 patients with R/R HCL treated with vemurafenib from the United States arm of the phase 2 clinical trial (NCT01711632). The best overall response rate was 86%, including 33% complete response (CR) and 53% partial response (PR). After a median follow-up of 40 months, 21 of 31 responders (68%) experienced relapse with a median relapse-free survival (RFS) of 19 months (range, 12.5-53.9 months). There was no significant difference in the RFS for patients with CR vs PR. Fourteen of 21 (67%) relapsed patients were retreated with vemurafenib, with 86% achieving complete hematologic response. Two patients acquired resistance to vemurafenib with the emergence of new KRAS and CDKN2A mutations, respectively. Six of 12 (50%) responders to vemurafenib retreatment experienced another relapse with a median RFS of 12.7 months. Overall survival (OS) was 82% at 4 years, with a significantly shorter OS in patients who relapsed within 1 year of initial treatment with vemurafenib. Higher cumulative doses or a longer duration of treatment did not lengthen the durability of response. All adverse events in the retreatment cohort were grade 1/2 except for 1 case of a grade 3 rash and 1 grade 3 fever/pneumonia. Our data suggest that vemurafenib retreatment is a safe and effective option for patients with R/R HCL.
    MeSH term(s) Humans ; Vemurafenib/therapeutic use ; Leukemia, Hairy Cell/drug therapy ; Leukemia, Hairy Cell/genetics ; Proto-Oncogene Proteins B-raf/genetics ; Protein Kinase Inhibitors/therapeutic use ; Remission Induction ; Antineoplastic Agents/adverse effects
    Chemical Substances Vemurafenib (207SMY3FQT) ; Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; Protein Kinase Inhibitors ; Antineoplastic Agents
    Language English
    Publishing date 2022-08-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood.2022016183
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.140: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 364: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Pediatric-inspired chemotherapy incorporating pegaspargase is safe and results in high rates of minimal residual disease negativity in adults up to age 60 with Philadelphia chromosome-negative acute lymphoblastic leukemia.

    Geyer, Mark B / Ritchie, Ellen K / Rao, Arati V / Vemuri, Shreya / Flynn, Jessica / Hsu, Meier / Devlin, Sean M / Roshal, Mikhail / Gao, Qi / Shukla, Madhulika / Salcedo, Jose M / Maslak, Peter / Tallman, Martin S / Douer, Dan / Park, Jae H

    Haematologica

    2021  Volume 106, Issue 8, Page(s) 2086–2094

    Abstract: Administration of pediatric-inspired chemotherapy to adults up to age 60 with acute lymphoblastic leukemia (ALL) is challenging in part due to toxicities of asparaginase as well as myelosuppression. We conducted a multicenter phase II clinical trial ( ... ...

    Abstract Administration of pediatric-inspired chemotherapy to adults up to age 60 with acute lymphoblastic leukemia (ALL) is challenging in part due to toxicities of asparaginase as well as myelosuppression. We conducted a multicenter phase II clinical trial (NCT01920737) investigating a pediatric-inspired regimen, based on the augmented arm of the Children's Cancer Group 1882 protocol, incorporating 6 doses of pegaspargase 2000 IU/m2, rationally synchronized to avoid overlapping toxicity with other agents. We treated 39 adults ages 20-60 years (median, 38 years) with newly-diagnosed ALL (n=31) or lymphoblastic lymphoma (n=8). Grade 3-4 hyperbilirubinemia occurred frequently and at higher rates in patients 40-60 (n=18) vs 18-39 (n=21) years (44 vs 10%, p=0.025). However, 8/9 patients re-challenged with pegaspargase did not experience recurrent grade 3-4 hyperbilirubinemia. Grade 3-4 hypertriglyceridemia and hypofibrinogenemia were common (each 59%). Asparaginase activity at 7-days post-infusion reflected levels associated with adequate asparagine depletion, even among those with antibodies to pegaspargase. Complete response (CR)/CR with incomplete hematologic recovery was observed post-induction in 38/39 (97%) patients. Among patients with ALL, rates of MRD negativity by multiparameter flow cytometry were 33% and 83% following Induction Phase I and Phase II, respectively. Event-free and overall survival at 3 years (67.8 and 76.4%) compare favorably to outcomes observed in other series. These results demonstrate pegaspargase can be administered in the context of intensive multi-agent chemotherapy to adults age ≤60 with manageable toxicity. This regimen may serve as an effective backbone into which novel agents may be incorporated in future frontline studies.
    MeSH term(s) Adult ; Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Asparaginase/adverse effects ; Child ; Humans ; Middle Aged ; Neoplasm, Residual ; Philadelphia Chromosome ; Polyethylene Glycols/adverse effects ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Young Adult
    Chemical Substances Polyethylene Glycols (3WJQ0SDW1A) ; pegaspargase (7D96IR0PPM) ; Asparaginase (EC 3.5.1.1)
    Language English
    Publishing date 2021-08-01
    Publishing country Italy
    Document type Journal Article ; Multicenter Study
    ZDB-ID 2333-4
    ISSN 1592-8721 ; 0017-6567 ; 0390-6078
    ISSN (online) 1592-8721
    ISSN 0017-6567 ; 0390-6078
    DOI 10.3324/haematol.2020.251686
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.76: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Scope of algae as third generation biofuels.

    Behera, Shuvashish / Singh, Richa / Arora, Richa / Sharma, Nilesh Kumar / Shukla, Madhulika / Kumar, Sachin

    Frontiers in bioengineering and biotechnology

    2015  Volume 2, Page(s) 90

    Abstract: An initiative has been taken to develop different solid, liquid, and gaseous biofuels as the alternative energy resources. The current research and technology based on the third generation biofuels derived from algal biomass have been considered as the ... ...

    Abstract An initiative has been taken to develop different solid, liquid, and gaseous biofuels as the alternative energy resources. The current research and technology based on the third generation biofuels derived from algal biomass have been considered as the best alternative bioresource that avoids the disadvantages of first and second generation biofuels. Algal biomass has been investigated for the implementation of economic conversion processes producing different biofuels such as biodiesel, bioethanol, biogas, biohydrogen, and other valuable co-products. In the present review, the recent findings and advance developments in algal biomass for improved biofuel production have been explored. This review discusses about the importance of the algal cell contents, various strategies for product formation through various conversion technologies, and its future scope as an energy security.
    Language English
    Publishing date 2015-02-11
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2014.00090
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top