Article: Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells
Phytomedicine. 2015 July 15, v. 22
2015
Abstract: Glycyrrhizin, silymarin, and ursodeoxycholic acid are widely used hepatoprotectants for the treatment of liver disorders, such as hepatitis C virus infection, primary biliary cirrhosis, and hepatocellular carcinoma.The gene expression profiles of HepG2 ... ...
| Abstract | Glycyrrhizin, silymarin, and ursodeoxycholic acid are widely used hepatoprotectants for the treatment of liver disorders, such as hepatitis C virus infection, primary biliary cirrhosis, and hepatocellular carcinoma.The gene expression profiles of HepG2 cells responsive to glycyrrhizin, silymarin, and ursodeoxycholic acid were analyzed in this study.HepG2 cells were treated with 25 µM hepatoprotectants for 24 h. Gene expression profiles of hepatoprotectants-treated cells were analyzed by oligonucleotide microarray in triplicates. Nuclear factor-κB (NF-κB) activities were assessed by luciferase assay.Among a total of 30,968 genes, 252 genes were commonly regulated by glycyrrhizin, silymarin, and ursodeoxycholic acid. These compounds affected the expression of genes relevant various biological pathways, such as neurotransmission, and glucose and lipid metabolism. Genes involved in hepatocarcinogenesis, apoptosis, and anti-oxidative pathways were differentially regulated by all compounds. Moreover, interaction networks showed that NF-κB might play a central role in the regulation of gene expression. Further analysis revealed that these hepatoprotectants inhibited NF-κB activities in a dose-dependent manner.Our data suggested that glycyrrhizin, silymarin, and ursodeoxycholic acid regulated the expression of genes relevant to apoptosis and oxidative stress in HepG2 cells. Moreover, the regulation by these hepatoprotectants might be relevant to the suppression of NF-κB activities. |
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| Keywords | apoptosis ; dose response ; gene expression ; gene expression regulation ; genes ; glucose ; glycyrrhizin ; Hepatitis C virus ; human cell lines ; lipid metabolism ; liver ; luciferase ; microarray technology ; oligonucleotides ; oxidative stress ; silymarin ; transcription factor NF-kappa B ; ursodeoxycholic acid | |||||
| Language | English | |||||
| Dates of publication | 2015-0715 | |||||
| Size | p. 768-777. | |||||
| Publishing place | Elsevier GmbH | |||||
| Document type | Article | |||||
| ZDB-ID | 1205240-1 | |||||
| ISSN | 1618-095X ; 0944-7113 | |||||
| ISSN (online) | 1618-095X | |||||
| ISSN | 0944-7113 | |||||
| DOI | 10.1016/j.phymed.2015.05.053 | |||||
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| Database | NAL-Catalogue (AGRICOLA) |
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