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  1. Article ; Online: Matrix metalloproteinase-2 and matrix metalloproteinase-9 in mice with ocular toxocariasis.

    Shyu, Ling-Yuh / Chen, Ke-Min / Lai, Shih-Chan

    Parasitology research

    2019  Volume 118, Issue 2, Page(s) 483–491

    Abstract: In ocular toxocariasis, Toxocara canis-induced inflammatory reaction can lead to eye destruction and granuloma, which is formed by immune cell infiltration and concurrent extensive remodeling tissue. Herein, the histomorphology of granuloma and ... ...

    Abstract In ocular toxocariasis, Toxocara canis-induced inflammatory reaction can lead to eye destruction and granuloma, which is formed by immune cell infiltration and concurrent extensive remodeling tissue. Herein, the histomorphology of granuloma and proteinase production in the eye of T. canis-infected BALB/c mice were investigated. Pathological effects substantially increased after the infection culminated in a severe leukocyte infiltration and granuloma formation from days 4 to 56 post-inoculation. The matrix metalloproteinase (MMP)-2 and MMP-9 activities remarkably increased, compared with those of uninfected control, by gelatin zymography and Western blot analysis in ocular toxocariasis. Granuloma formation had a remarkably positive correlation with MMP-2 and MMP-9 levels. We suggested that T. canis larvae and leukocytes infiltrated from blood vessel both migrated into corpus adiposum orbitae. Activated leukocytes secreted MMP-2 and MMP-9, leading to fibronectin degradation. The imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 may play a role in inflammatory cell infiltration and extracellular matrix degradation, forming granuloma, in ophthalmological pathogenesis of T. canis infection.
    MeSH term(s) Animals ; Blotting, Western ; Fibronectins/metabolism ; Granuloma/enzymology ; Granuloma/etiology ; Granuloma/physiopathology ; Inflammation ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/metabolism ; Mice ; Mice, Inbred BALB C ; Tissue Inhibitor of Metalloproteinase-1/metabolism ; Tissue Inhibitor of Metalloproteinase-2/metabolism ; Toxocara canis/immunology ; Toxocara canis/metabolism ; Toxocariasis/complications ; Toxocariasis/enzymology
    Chemical Substances Fibronectins ; Tissue Inhibitor of Metalloproteinase-1 ; Tissue Inhibitor of Metalloproteinase-2 (127497-59-0) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 2019-01-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-018-06196-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Regulation of Proinflammatory Enzymes by Peroxisome Proliferator-Activated Receptor Gamma in Astroglia Infected with Toxoplasma gondii.

    Shyu, Ling-Yuh / Chen, Ke-Min / Lu, Cheng-You / Lai, Shih-Chan

    The Journal of parasitology

    2020  Volume 106, Issue 5, Page(s) 564–571

    Abstract: Peroxisome proliferator-activated receptor gamma (PPARγ) regulates neuroinflammation, and its agonists act as neuroprotective agents. This study aims to investigate the correlation between PPARγ and proinflammatory enzyme expression in astroglia infected ...

    Abstract Peroxisome proliferator-activated receptor gamma (PPARγ) regulates neuroinflammation, and its agonists act as neuroprotective agents. This study aims to investigate the correlation between PPARγ and proinflammatory enzyme expression in astroglia infected with Toxoplasma gondii tachyzoite in vitro. Our results showed that matrix metalloprotease (MMP)-2, MMP-9, cyclooxygenase-2 (COX-2), prostaglandin (PGE)-2, inducible nitric-oxide synthase (iNOS), and nitric oxide (NO) were significantly increased in T. gondii-infected astroglia. Furthermore, the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO were significantly decreased by rosiglitazone-a PPARγ agonist. By contrast, the treatment with GW9662, a PPARγ antagonist, efficiently increased the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO. These results suggested that the treatment with rosiglitazone offers a potential strategy for controlling the inflammatory factors in T. gondii infection.
    MeSH term(s) Animals ; Astrocytes/enzymology ; Astrocytes/parasitology ; Brain/cytology ; Cell Line ; Cyclooxygenase 2/metabolism ; Dinoprostone/metabolism ; Fibroblasts/parasitology ; Foreskin/cytology ; Humans ; Male ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/metabolism ; Nitric Oxide/analysis ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; PPAR gamma/physiology ; Rabbits ; Rosiglitazone/pharmacology ; Toxoplasma/physiology
    Chemical Substances PPAR gamma ; Rosiglitazone (05V02F2KDG) ; Nitric Oxide (31C4KY9ESH) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Dinoprostone (K7Q1JQR04M)
    Language English
    Publishing date 2020-08-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 300870-8
    ISSN 1937-2345 ; 0022-3395
    ISSN (online) 1937-2345
    ISSN 0022-3395
    DOI 10.1645/18-184
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Angiostrongylus cantonensis infection induces MMP-9 and causes tight junction protein disruption associated with Purkinje cell degeneration.

    Lai, Shih-Chan / Lu, Cheng-You / Shyu, Ling-Yuh / Chen, Ke-Min

    Parasitology research

    2020  Volume 119, Issue 10, Page(s) 3433–3441

    Abstract: Angiostrongylus cantonensis causes a human central nervous system (CNS) infection characterized by eosinophilic meningitis or meningoencephalitis. Individuals infected with A. cantonensis exhibit unbalanced walking. The mechanism of extensive ... ...

    Abstract Angiostrongylus cantonensis causes a human central nervous system (CNS) infection characterized by eosinophilic meningitis or meningoencephalitis. Individuals infected with A. cantonensis exhibit unbalanced walking. The mechanism of extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. Tight junction proteins (e.g., claudin-5 and zonula occludens-1) are the most important regulators of paracellular permeability and cellular adhesion. In a previous study, we found that increased matrix metalloproteinase-9 (MMP-9) activity may be associated with blood-CNS barrier disruption and/or the degeneration of Purkinje cells in eosinophilic meningitis caused by A. cantonensis. In the present study, the co-localization of MMP-9 and tight junction proteins on the degeneration of Purkinje cells was measured via confocal laser scanning immunofluorescence microscopy. The statistical evidence indicated that MMP-9 correlated between tight junction protein disruption and Purkinje cell degeneration at 20 days post-infection with A. cantonensis. In conclusion, Purkinje cell degeneration is highly correlated with tight junction protein disruption via the MMP-9 activation pathway.
    MeSH term(s) Angiostrongylus cantonensis/physiology ; Animals ; Disease Models, Animal ; Larva/physiology ; Matrix Metalloproteinase 9/metabolism ; Mice ; Purkinje Cells/metabolism ; Purkinje Cells/parasitology ; Purkinje Cells/pathology ; Strongylida Infections/metabolism ; Strongylida Infections/parasitology ; Strongylida Infections/pathology ; Tight Junction Proteins/metabolism
    Chemical Substances Tight Junction Proteins ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 2020-08-13
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-020-06840-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Peroxisome-Proliferator Activator Receptor γ in Mouse Model with Meningoencephalitis Caused by Angiostrongylus cantonensis.

    Chen, Ke-Min / Peng, Chi-Yang / Shyu, Ling-Yuh / Lan, Kuang-Pin / Lai, Shih-Chan

    The Journal of parasitology

    2021  Volume 107, Issue 2, Page(s) 205–213

    Abstract: Peroxisome-proliferator activator receptor γ (PPARγ) has an anti-inflammatory role that inhibits the nuclear factor-κB (NF-κB) pathway and regulates the expressions of pro-inflammatory proteins, whereas its role in parasitic meningoencephalitis remains ... ...

    Abstract Peroxisome-proliferator activator receptor γ (PPARγ) has an anti-inflammatory role that inhibits the nuclear factor-κB (NF-κB) pathway and regulates the expressions of pro-inflammatory proteins, whereas its role in parasitic meningoencephalitis remains unknown. In this study we investigated the role of PPARγ and related mechanisms in eosinophilic meningoencephalitis caused by the rat lungworm Angiostrongylus cantonensis. We observed increased protein NF-κB expression in mouse brain tissue using GW9662, which is the specific antagonist of PPARγ, in a mouse model of angiostrongyliasis. Then we investigated NF-κB-related downstream proteins, such as COX-2, NOSs, and IL-1β, with Western blot or enzyme-linked immunosorbent assay and found that the protein expression was upregulated. The results of gelatin zymography also showed that the MMP-9 activities were upregulated. Treatment with GW9662 increased the permeability of the blood-brain barrier and the number of eosinophils in cerebrospinal fluid. These results suggested that in angiostrongyliasis, PPARγ may play an anti-inflammation role in many inflammatory mediators, including NOS-related oxidative stress, cytokines, and matrix metalloproteinase cascade by decreasing the NF-κB action.
    MeSH term(s) Angiostrongylus cantonensis/pathogenicity ; Animals ; Blood-Brain Barrier/metabolism ; Cyclooxygenase 2/metabolism ; Disease Models, Animal ; Male ; Matrix Metalloproteinase 2/cerebrospinal fluid ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/cerebrospinal fluid ; Matrix Metalloproteinase 9/metabolism ; Meningoencephalitis/metabolism ; Meningoencephalitis/parasitology ; Mice ; Mice, Inbred BALB C ; PPAR gamma/metabolism ; Random Allocation ; Strongylida Infections/metabolism ; Strongylida Infections/parasitology ; Taiwan
    Chemical Substances PPAR gamma ; Cyclooxygenase 2 (EC 1.14.99.1) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 2021-03-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 300870-8
    ISSN 1937-2345 ; 0022-3395
    ISSN (online) 1937-2345
    ISSN 0022-3395
    DOI 10.1645/19-182
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Regulation of Proinflammatory Enzymes by Peroxisome Proliferator–Activated Receptor Gamma in Astroglia Infected with Toxoplasma gondii

    Shyu, Ling-Yuh / Chen, Ke-Min / Lu, Cheng-You / Lai, Shih-Chan

    Journal of parasitology. 2020 Sept. 8, v. 106, no. 5

    2020  

    Abstract: Peroxisome proliferator–activated receptor gamma (PPARγ) regulates neuroinflammation, and its agonists act as neuroprotective agents. This study aims to investigate the correlation between PPARγ and proinflammatory enzyme expression in astroglia infected ...

    Abstract Peroxisome proliferator–activated receptor gamma (PPARγ) regulates neuroinflammation, and its agonists act as neuroprotective agents. This study aims to investigate the correlation between PPARγ and proinflammatory enzyme expression in astroglia infected with Toxoplasma gondii tachyzoite in vitro. Our results showed that matrix metalloprotease (MMP)-2, MMP-9, cyclooxygenase-2 (COX-2), prostaglandin (PGE)-2, inducible nitric-oxide synthase (iNOS), and nitric oxide (NO) were significantly increased in T. gondii–infected astroglia. Furthermore, the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO were significantly decreased by rosiglitazone—a PPARγ agonist. By contrast, the treatment with GW9662, a PPARγ antagonist, efficiently increased the expression levels of MMP-2, MMP-9, COX-2, PGE-2, iNOS, and NO. These results suggested that the treatment with rosiglitazone offers a potential strategy for controlling the inflammatory factors in T. gondii infection.
    Keywords Toxoplasma gondii ; agonists ; antagonists ; astrocytes ; metalloproteinases ; nitric oxide ; parasitology ; peroxisome proliferator-activated receptor gamma ; prostaglandin synthase ; prostaglandins ; tachyzoites
    Language English
    Dates of publication 2020-0908
    Size p. 564-571.
    Publishing place American Society of Parasitologists
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 300870-8
    ISSN 1937-2345 ; 0022-3395
    ISSN (online) 1937-2345
    ISSN 0022-3395
    DOI 10.1645/18-184
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Angiostrongylus cantonensis infection induces MMP-9 and causes tight junction protein disruption associated with Purkinje cell degeneration

    Lai, Shih-Chan / Lu, Cheng-You / Shyu, Ling-Yuh / Chen, Ke-Min

    Parasitology research. 2020 Oct., v. 119, no. 10

    2020  

    Abstract: Angiostrongylus cantonensis causes a human central nervous system (CNS) infection characterized by eosinophilic meningitis or meningoencephalitis. Individuals infected with A. cantonensis exhibit unbalanced walking. The mechanism of extensive ... ...

    Abstract Angiostrongylus cantonensis causes a human central nervous system (CNS) infection characterized by eosinophilic meningitis or meningoencephalitis. Individuals infected with A. cantonensis exhibit unbalanced walking. The mechanism of extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. Tight junction proteins (e.g., claudin-5 and zonula occludens-1) are the most important regulators of paracellular permeability and cellular adhesion. In a previous study, we found that increased matrix metalloproteinase-9 (MMP-9) activity may be associated with blood–CNS barrier disruption and/or the degeneration of Purkinje cells in eosinophilic meningitis caused by A. cantonensis. In the present study, the co-localization of MMP-9 and tight junction proteins on the degeneration of Purkinje cells was measured via confocal laser scanning immunofluorescence microscopy. The statistical evidence indicated that MMP-9 correlated between tight junction protein disruption and Purkinje cell degeneration at 20 days post-infection with A. cantonensis. In conclusion, Purkinje cell degeneration is highly correlated with tight junction protein disruption via the MMP-9 activation pathway.
    Keywords Parastrongylus cantonensis ; cell adhesion ; central nervous system ; fluorescence microscopy ; gelatinase B ; humans ; meningoencephalitis ; parasitology ; permeability ; research ; tight junctions
    Language English
    Dates of publication 2020-10
    Size p. 3433-3441.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-020-06840-y
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  7. Article: Peroxisome-Proliferator Activator Receptor γ in Mouse Model with Meningoencephalitis Caused by Angiostrongylus cantonensis

    Chen, Ke-Min / Peng, Chi-Yang / Shyu, Ling-Yuh / Lan, Kuang-Pin / Lai, Shih-Chan

    Journal of parasitology. 2021 Mar. 8, v. 107, no. 2

    2021  

    Abstract: Peroxisome-proliferator activator receptor γ (PPARγ) has an anti-inflammatory role that inhibits the nuclear factor-jB (NF-jB) pathway and regulates the expressions of pro-inflammatory proteins, whereas its role in parasitic meningoencephalitis remains ... ...

    Abstract Peroxisome-proliferator activator receptor γ (PPARγ) has an anti-inflammatory role that inhibits the nuclear factor-jB (NF-jB) pathway and regulates the expressions of pro-inflammatory proteins, whereas its role in parasitic meningoencephalitis remains unknown. In this study we investigated the role of PPARγ and related mechanisms in eosinophilic meningoencephalitis caused by the rat lungworm Angiostrongylus cantonensis. We observed increased protein NF-jB expression in mouse brain tissue using GW9662, which is the specific antagonist of PPARγ, in a mouse model of angiostrongyliasis. Then we investigated NF-jB-related downstream proteins, such as COX-2, NOSs, and IL-1b, with Western blot or enzyme-linked immunosorbent assay and found that the protein expression was upregulated. The results of gelatin zymography also showed that the MMP-9 activities were upregulated. Treatment with GW9662 increased the permeability of the blood-brain barrier and the number of eosinophils in cerebrospinal fluid. These results suggested that in angiostrongyliasis, PPARγ may play an anti-inflammation role in many inflammatory mediators, including NOS-related oxidative stress, cytokines, and matrix metalloproteinase cascade by decreasing the NF-jB action.
    Keywords Parastrongylus cantonensis ; Western blotting ; antagonists ; blood-brain barrier ; brain ; cerebrospinal fluid ; cytokines ; enzyme-linked immunosorbent assay ; eosinophils ; gelatin ; lungworms ; meningoencephalitis ; metalloproteinases ; mice ; oxidative stress ; parasitology ; permeability ; protein synthesis ; rats
    Language English
    Dates of publication 2021-0308
    Size p. 205-213.
    Publishing place American Society of Parasitologists
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 300870-8
    ISSN 1937-2345 ; 0022-3395
    ISSN (online) 1937-2345
    ISSN 0022-3395
    DOI 10.1645/19-182
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  8. Article: Matrix metalloproteinase-2 and matrix metalloproteinase-9 in mice with ocular toxocariasis

    Shyu, Ling-Yuh / Ke-Min Chen / Shih-Chan Lai

    Parasitology research. 2019 Feb., v. 118, no. 2

    2019  

    Abstract: In ocular toxocariasis, Toxocara canis-induced inflammatory reaction can lead to eye destruction and granuloma, which is formed by immune cell infiltration and concurrent extensive remodeling tissue. Herein, the histomorphology of granuloma and ... ...

    Abstract In ocular toxocariasis, Toxocara canis-induced inflammatory reaction can lead to eye destruction and granuloma, which is formed by immune cell infiltration and concurrent extensive remodeling tissue. Herein, the histomorphology of granuloma and proteinase production in the eye of T. canis-infected BALB/c mice were investigated. Pathological effects substantially increased after the infection culminated in a severe leukocyte infiltration and granuloma formation from days 4 to 56 post-inoculation. The matrix metalloproteinase (MMP)-2 and MMP-9 activities remarkably increased, compared with those of uninfected control, by gelatin zymography and Western blot analysis in ocular toxocariasis. Granuloma formation had a remarkably positive correlation with MMP-2 and MMP-9 levels. We suggested that T. canis larvae and leukocytes infiltrated from blood vessel both migrated into corpus adiposum orbitae. Activated leukocytes secreted MMP-2 and MMP-9, leading to fibronectin degradation. The imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 may play a role in inflammatory cell infiltration and extracellular matrix degradation, forming granuloma, in ophthalmological pathogenesis of T. canis infection.
    Keywords Toxocara canis ; Western blotting ; blood vessels ; extracellular matrix ; eyes ; fibronectins ; gelatin ; gelatinase A ; gelatinase B ; granuloma ; larvae ; leukocytes ; mice ; pathogenesis ; toxocariasis
    Language English
    Dates of publication 2019-02
    Size p. 483-491.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-018-06196-4
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    In stock of ZB MED Bonn / Germany

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  9. Article ; Online: Proteasome serves as pivotal regulator in Angiostrongylus cantonensis-induced eosinophilic meningoencephalitis.

    Chen, An-Chih / Shyu, Ling-Yuh / Lin, Yi-Chieh / Chen, Ke-Min / Lai, Shih-Chan

    PloS one

    2019  Volume 14, Issue 8, Page(s) e0220503

    Abstract: Proteasome primarily degrades the unneeded or damaged proteins by proteolysis. Disruption of the brain barrier and its resulting meningoencephalitis caused by Angiostrongylus cantonensis are important pathological events in non-permissive hosts. In this ... ...

    Abstract Proteasome primarily degrades the unneeded or damaged proteins by proteolysis. Disruption of the brain barrier and its resulting meningoencephalitis caused by Angiostrongylus cantonensis are important pathological events in non-permissive hosts. In this study, the results showed upregulated proteasome during A. cantonensis infection. Occludin degradation and matrix metalloproteinase-9 (MMP-9) activity were significantly increased in infected mice than in uninfected mice. Moreover, confocal immunoflourescence microscopy showed that occludin was co-localized with MMP-9. The infected-mice were treated with proteasomal activity inhibitor MG132 by 1.5 and 3.0 mg/kg/day, which resulted in significantly reduced protein levels of phosphorylated IκBα (P<0.05) compared with the untreated control. The phosphorylated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) showed similar result. In addition, MMP-9 activity and occludin degradation were reduced because of MG132 treatment. These results suggested that the proteasome in A. cantonensis infection degraded phosphorylated IκBα, modulated phosphorylated NF-κB, and then regulated the activation of MMP-9 and occludin degradation. Proteasome alterations were presented in eosinophilic meningitis of BALB/c mice and may contribute to the pathophysiology of eosinophilic meningitis by increasing occludin degradation. This molecule would serve as pivotal regulator in A. cantonensis-induced eosinophilic meningoencephalitis.
    MeSH term(s) Angiostrongylus cantonensis ; Animals ; Brain/drug effects ; Brain/metabolism ; Cysteine Proteinase Inhibitors/pharmacology ; Disease Models, Animal ; Leupeptins/pharmacology ; Male ; Matrix Metalloproteinase 9/metabolism ; Meningoencephalitis/metabolism ; Meningoencephalitis/parasitology ; Mice ; NF-kappa B/metabolism ; Occludin/metabolism ; Phosphorylation ; Proteasome Endopeptidase Complex/metabolism ; Strongylida Infections/metabolism ; Up-Regulation
    Chemical Substances Cysteine Proteinase Inhibitors ; Leupeptins ; NF-kappa B ; Occludin ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Proteasome Endopeptidase Complex (EC 3.4.25.1) ; benzyloxycarbonylleucyl-leucyl-leucine aldehyde (RF1P63GW3K)
    Language English
    Publishing date 2019-08-15
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0220503
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Resveratrol relieves Angiostrongylus cantonensis - Induced meningoencephalitis by activating sirtuin-1.

    Chen, An-Chih / Shyu, Ling-Yuh / Hsin, Yue-Loong / Chen, Ke-Min / Lai, Shih-Chan

    Acta tropica

    2017  Volume 173, Page(s) 76–84

    Abstract: Resveratrol, a natural herbal compound found in high levels in grapes and red wine, is frequently used as activator of sirtuin-1. This study investigated the potential function of sirtuin-1 in regulating angiostrongyliasis meningoencephalitis in ... ...

    Abstract Resveratrol, a natural herbal compound found in high levels in grapes and red wine, is frequently used as activator of sirtuin-1. This study investigated the potential function of sirtuin-1 in regulating angiostrongyliasis meningoencephalitis in resveratrol-treated mice. Mice were subjected to meningoencephalitis to study the protective effect of resveratrol against meningoencephalitis and investigate the effects of sirtuin-1 activation on brain. Results demonstrated that sirtuin-1 level decreased in mice with meningoencephalitis and significantly increased in resveratrol-treated mice. Moreover, resveratrol treatment significantly reduced eosinophil counts, p65, Interferon-γ, interleukin (IL)-5, IL-33, and tumor necrosis factor-α levels, matrix metalloproteinase-9 activity, claudin-5 degradation, and blood-brain barrier permeability. By contrast, the anti-inflammatory factor IL-10 was significantly increased in resveratrol-treated mice. Resveratrol treatment was partially beneficial in controlling the pathological processes of angiostrongyliasis meningoencephalitis. The results demonstrate the neuroprotective and anti-inflammatory effects of resveratrol against Angiostrongylus cantonensis-induced eosinophilic meningoencephalitis in mice. Treatment with sirtuin-1 agonist was given within a therapeutic window after A. cantonensis infection.
    Language English
    Publishing date 2017-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2017.05.023
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