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  1. Article ; Online: IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo.

    Sierakowiak, Adam / Henriques-Normark, Birgitta / Iovino, Federico

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1968, Page(s) 195–202

    Abstract: Imaging through the IVIS Spectrum CT system does not provide the resolution at cellular level like the high-resolution or super-resolution microscopy. Rather, it detects bacterial infections in specific anatomical compartments/organs of the animals. The ... ...

    Abstract Imaging through the IVIS Spectrum CT system does not provide the resolution at cellular level like the high-resolution or super-resolution microscopy. Rather, it detects bacterial infections in specific anatomical compartments/organs of the animals. The IVIS Spectrum imaging system is a unique imaging technology that allows for real-time monitoring of disease progression in living animals through the use of either bioluminescent or fluorescent probes.
    MeSH term(s) Animals ; Bacterial Infections/diagnosis ; Luminescent Measurements/methods ; Pneumococcal Infections/diagnosis ; Tomography, X-Ray Computed/methods
    Language English
    Publishing date 2019-03-30
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9199-0_16
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  2. Article ; Online: Cytotoxic Alkylynols of the Sponge

    Kovalerchik, Dimitry / Zovko, Ana / Hååg, Petra / Sierakowiak, Adam / Viktorsson, Kristina / Lewensohn, Rolf / Ilan, Micha / Carmeli, Shmuel

    Marine drugs

    2022  Volume 20, Issue 4

    Abstract: A series of twenty-three linear and branched chain mono acetylene lipids were isolated from the Caribbean Sea ... ...

    Abstract A series of twenty-three linear and branched chain mono acetylene lipids were isolated from the Caribbean Sea sponge
    MeSH term(s) Acetylene/therapeutic use ; Alkanes ; Antineoplastic Agents/chemistry ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/metabolism ; Humans ; Lung Neoplasms/drug therapy ; Lung Neoplasms/metabolism ; Structure-Activity Relationship
    Chemical Substances Alkanes ; Antineoplastic Agents ; Acetylene (OC7TV75O83)
    Language English
    Publishing date 2022-04-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md20040265
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  3. Article ; Online: Default mode network, motor network, dorsal and ventral basal ganglia networks in the rat brain: comparison to human networks using resting state-fMRI.

    Sierakowiak, Adam / Monnot, Cyril / Aski, Sahar Nikkhou / Uppman, Martin / Li, Tie-Qiang / Damberg, Peter / Brené, Stefan

    PloS one

    2015  Volume 10, Issue 3, Page(s) e0120345

    Abstract: Rodent models are developed to enhance understanding of the underlying biology of different brain disorders. However, before interpreting findings from animal models in a translational aspect to understand human disease, a fundamental step is to first ... ...

    Abstract Rodent models are developed to enhance understanding of the underlying biology of different brain disorders. However, before interpreting findings from animal models in a translational aspect to understand human disease, a fundamental step is to first have knowledge of similarities and differences of the biological systems studied. In this study, we analyzed and verified four known networks termed: default mode network, motor network, dorsal basal ganglia network, and ventral basal ganglia network using resting state functional MRI (rsfMRI) in humans and rats. Our work supports the notion that humans and rats have common robust resting state brain networks and that rsfMRI can be used as a translational tool when validating animal models of brain disorders. In the future, rsfMRI may be used, in addition to short-term interventions, to characterize longitudinal effects on functional brain networks after long-term intervention in humans and rats.
    MeSH term(s) Adult ; Animals ; Basal Ganglia/physiology ; Brain/diagnostic imaging ; Brain/physiology ; Brain Diseases/physiopathology ; Brain Mapping ; Humans ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging ; Male ; Models, Animal ; Nerve Net/physiology ; Radiography ; Rats ; Rats, Sprague-Dawley
    Language English
    Publishing date 2015-03-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0120345
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  4. Article: MRI evidence of endolymphatic impermeability to the gadolinium molecule in the in vivo mouse inner ear at 9.4 tesla.

    Counter, S Allen / Nikkhou, Sahar / Brené, Stefan / Damberg, Peter / Sierakowiak, Adam / Klason, Tomas / Berglin, Cecilia Engmér / Laurell, Göran

    The open neuroimaging journal

    2013  Volume 7, Page(s) 27–31

    Abstract: Objective: Previous in vivo experimental magnetic resonance imaging (MRI) investigations of the mammalian inner ear at 4.7 Tesla have indicated that intravenously injected gadolinium (Gd) penetrates the perilymphatic labyrinth, but not the endolymphatic ...

    Abstract Objective: Previous in vivo experimental magnetic resonance imaging (MRI) investigations of the mammalian inner ear at 4.7 Tesla have indicated that intravenously injected gadolinium (Gd) penetrates the perilymphatic labyrinth, but not the endolymphatic membranous labyrinth. In the present study, high field MRI at 9.4T was used to visualize the in vivo mouse vestibulo-cochlea system, and to determine whether the endolymphatic system is permeable to a Gd complex.
    Methods: A 9.4 T Varian magnet equipped with a 12 cm inner diameter gradient system with maximum gradient strength of 600 mT/m, a millipede coil (Varian design) and a Gd contrast agent were used for image acquisition in the normal C57 BL-6 mouse.
    Results: High-resolution 2D and 3D images of the mouse cochlea were acquired within 80 minutes following intravenous injection of Gd. Gd initially permeated the perilymphatic scala tympani and scala vestibuli, and permitted visualization of both cochlear turns from base to apex. The superior, inferior and lateral semicircular canals were subsequently visualized in 3 planes. The membranous endolymphatic labyrinth was impermeable to intravenously injected Gd, and thus showed no apparent uptake of Gd at 9.4T.
    Conclusion: The 9.4T field strength MRI permitted acquisition of high resolution images of anatomical and physiological features of the normal, wild type mouse perilymphatic inner ear in vivo, and provided further evidence that the endolymphatic system is impermeable to intravenously injected Gd.
    Language English
    Publishing date 2013-06-28
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2395976-9
    ISSN 1874-4400
    ISSN 1874-4400
    DOI 10.2174/1874440001307010027
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  5. Article ; Online: Melphalan-flufenamide is cytotoxic and potentiates treatment with chemotherapy and the Src inhibitor dasatinib in urothelial carcinoma.

    Viktorsson, Kristina / Shah, Carl-Henrik / Juntti, Therese / Hååg, Petra / Zielinska-Chomej, Katarzyna / Sierakowiak, Adam / Holmsten, Karin / Tu, Jessica / Spira, Jack / Kanter, Lena / Lewensohn, Rolf / Ullén, Anders

    Molecular oncology

    2016  Volume 10, Issue 5, Page(s) 719–734

    Abstract: Background: Chemotherapy options in advanced urothelial carcinoma (UC) remain limited. Here we evaluated the peptide-based alkylating agent melphalan-flufenamide (mel-flufen) for UC.: Methods: UC cell lines J82, RT4, TCCsup and 5637 were treated with ...

    Abstract Background: Chemotherapy options in advanced urothelial carcinoma (UC) remain limited. Here we evaluated the peptide-based alkylating agent melphalan-flufenamide (mel-flufen) for UC.
    Methods: UC cell lines J82, RT4, TCCsup and 5637 were treated with mel-flufen, alone or combined with cisplatin, gemcitabine, dasatinib or bestatin. Cell viability (MTT assay), intracellular drug accumulation (liquid chromatography) apoptosis induction (apoptotic cell nuclei morphology, western blot analysis of PARP-1/caspase-9 cleavage and Bak/Bax activation) were evaluated. Kinome alterations were characterized by PathScan array and phospho-Src validated by western blotting. Aminopeptidase N (ANPEP) expression was evaluated in UC clinical specimens in relation to patient outcome.
    Results: In J82, RT4, TCCsup and 5637 UC cells, mel-flufen amplified the intracellular loading of melphalan in part via aminopeptidase N (ANPEP), resulting in increased cytotoxicity compared to melphalan alone. Mel-flufen induced apoptosis seen as activation of Bak/Bax, cleavage of caspase-9/PARP-1 and induction of apoptotic cell nuclei morphology. Combining mel-flufen with cisplatin or gemcitabine in J82 cells resulted in additive cytotoxic effects and for gemcitabine also increased apoptosis induction. Profiling of mel-flufen-induced kinome alterations in J82 cells revealed that mel-flufen alone did not inhibit Src phosphorylation. Accordingly, the Src inhibitor dasatinib sensitized for mel-flufen cytotoxicity. Immunohistochemical analysis of the putative mel-flufen biomarker ANPEP demonstrated prominent expression levels in tumours from 82 of 83 cystectomy patients. Significantly longer median overall survival was found in patients with high ANPEP expression (P = 0.02).
    Conclusion: Mel-flufen alone or in combination with cisplatin, gemcitabine or Src inhibition holds promise as a novel treatment for UC.
    MeSH term(s) Antineoplastic Agents, Alkylating/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Apoptosis/drug effects ; Cell Line, Tumor ; Dasatinib/pharmacology ; Humans ; Melphalan/analogs & derivatives ; Melphalan/pharmacology ; Phenylalanine/analogs & derivatives ; Phenylalanine/pharmacology ; Protein Kinase Inhibitors/pharmacology ; Urologic Neoplasms/drug therapy ; Urologic Neoplasms/pathology ; Urothelium/drug effects ; Urothelium/pathology ; bcl-2 Homologous Antagonist-Killer Protein/metabolism ; bcl-2-Associated X Protein/metabolism ; src-Family Kinases/antagonists & inhibitors
    Chemical Substances Antineoplastic Agents, Alkylating ; BAK1 protein, human ; Protein Kinase Inhibitors ; bcl-2 Homologous Antagonist-Killer Protein ; bcl-2-Associated X Protein ; melflufen (3412470A0V) ; Phenylalanine (47E5O17Y3R) ; src-Family Kinases (EC 2.7.10.2) ; Melphalan (Q41OR9510P) ; Dasatinib (RBZ1571X5H)
    Language English
    Publishing date 2016-01-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2415106-3
    ISSN 1878-0261 ; 1574-7891
    ISSN (online) 1878-0261
    ISSN 1574-7891
    DOI 10.1016/j.molonc.2015.12.013
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6637: Show issues Location:
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  6. Article: Hippocampal morphology in a rat model of depression: the effects of physical activity.

    Sierakowiak, Adam / Mattsson, Anna / Gómez-Galán, Marta / Feminía, Teresa / Graae, Lisette / Aski, Sahar Nikkhou / Damberg, Peter / Lindskog, Mia / Brené, Stefan / Åberg, Elin

    The open neuroimaging journal

    2015  Volume 9, Page(s) 1–6

    Abstract: Accumulating in vivo and ex vivo evidences show that humans suffering from depression have decreased hippocampal volume and altered spine density. Moreover, physical activity has an antidepressant effect in humans and in animal models, but to what extent ...

    Abstract Accumulating in vivo and ex vivo evidences show that humans suffering from depression have decreased hippocampal volume and altered spine density. Moreover, physical activity has an antidepressant effect in humans and in animal models, but to what extent physical activity can affect hippocampal volume and spine numbers in a model for depression is not known. In this study we analyzed whether physical activity affects hippocampal volume and spine density by analyzing a rodent genetic model of depression, Flinders Sensitive Line Rats (FSL), with Magnetic Resonance Imaging (MRI) and ex vivo Golgi staining. We found that physical activity in the form of voluntary wheel running during 5 weeks increased hippocampal volume. Moreover, runners also had larger numbers of thin spines in the dentate gyrus. Our findings support that voluntary wheel running, which is antidepressive in FSL rats, is associated with increased hippocampal volume and spine numbers.
    Language English
    Publishing date 2015-01-30
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2395976-9
    ISSN 1874-4400
    ISSN 1874-4400
    DOI 10.2174/1874440001509010001
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  7. Article ; Online: Orexin-corticotropin-releasing factor receptor heteromers in the ventral tegmental area as targets for cocaine.

    Navarro, Gemma / Quiroz, César / Moreno-Delgado, David / Sierakowiak, Adam / McDowell, Kimberly / Moreno, Estefanía / Rea, William / Cai, Ning-Sheng / Aguinaga, David / Howell, Lesley A / Hausch, Felix / Cortés, Antonio / Mallol, Josefa / Casadó, Vicent / Lluís, Carme / Canela, Enric I / Ferré, Sergi / McCormick, Peter J

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2015  Volume 35, Issue 17, Page(s) 6639–6653

    Abstract: Release of the neuropeptides corticotropin-releasing factor (CRF) and orexin-A in the ventral tegmental area (VTA) play an important role in stress-induced cocaine-seeking behavior. We provide evidence for pharmacologically significant interactions ... ...

    Abstract Release of the neuropeptides corticotropin-releasing factor (CRF) and orexin-A in the ventral tegmental area (VTA) play an important role in stress-induced cocaine-seeking behavior. We provide evidence for pharmacologically significant interactions between CRF and orexin-A that depend on oligomerization of CRF1 receptor (CRF1R) and orexin OX1 receptors (OX1R). CRF1R-OX1R heteromers are the conduits of a negative crosstalk between orexin-A and CRF as demonstrated in transfected cells and rat VTA, in which they significantly modulate dendritic dopamine release. The cocaine target σ1 receptor (σ1R) also associates with the CRF1R-OX1R heteromer. Cocaine binding to the σ1R-CRF1R-OX1R complex promotes a long-term disruption of the orexin-A-CRF negative crosstalk. Through this mechanism, cocaine sensitizes VTA cells to the excitatory effects of both CRF and orexin-A, thus providing a mechanism by which stress induces cocaine seeking.
    MeSH term(s) Animals ; Arrestins/metabolism ; Cocaine/pharmacology ; Cyclic AMP/metabolism ; Dendrites/drug effects ; Dendrites/metabolism ; Dopamine/metabolism ; Dopamine Uptake Inhibitors/pharmacology ; Gene Expression Regulation/drug effects ; HEK293 Cells ; Humans ; In Vitro Techniques ; MAP Kinase Signaling System/drug effects ; MAP Kinase Signaling System/physiology ; Male ; Oncogene Protein v-akt/metabolism ; Orexin Receptors/genetics ; Orexin Receptors/metabolism ; Phosphorylation/drug effects ; Protein Binding/drug effects ; Rats ; Rats, Sprague-Dawley ; Receptors, Corticotropin-Releasing Hormone/genetics ; Receptors, Corticotropin-Releasing Hormone/metabolism ; Time Factors ; Ventral Tegmental Area/cytology ; Ventral Tegmental Area/drug effects ; beta-Arrestins
    Chemical Substances Arrestins ; CRF receptor type 1 ; Dopamine Uptake Inhibitors ; Orexin Receptors ; Receptors, Corticotropin-Releasing Hormone ; beta-Arrestins ; Cyclic AMP (E0399OZS9N) ; Oncogene Protein v-akt (EC 2.7.11.1) ; Cocaine (I5Y540LHVR) ; Dopamine (VTD58H1Z2X)
    Language English
    Publishing date 2015-04-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.4364-14.2015
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1668: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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