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  1. Book: Vibrio cholerae

    Sikora, Aleksandra E.

    methods and protocols

    (Methods in molecular biology ; 1839 ; Springer protocols)

    2018  

    Author's details edited by Aleksandra E. Sikora
    Series title Methods in molecular biology ; 1839
    Springer protocols
    Collection
    Keywords Vibrio cholerae / Laboratory manuals ; Vibrio cholerae
    Language English
    Size x, 180 Seiten, Illustrationen
    Publisher Humana Press
    Publishing place New York
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT019861556
    ISBN 978-1-4939-8684-2 ; 9781493986859 ; 1-4939-8684-8 ; 1493986856
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1838- verfügbar in Königswinter Königswinter

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  2. Article ; Online: A Critical Need for Research on Gonorrhea Vaccine Acceptability.

    Harvey, S Marie / Gibbs, Susannah E / Sikora, Aleksandra E

    Sexually transmitted diseases

    2021  Volume 48, Issue 8, Page(s) e116–e118

    MeSH term(s) Bacterial Vaccines ; Gonorrhea/epidemiology ; Gonorrhea/prevention & control ; Humans ; Neisseria gonorrhoeae
    Chemical Substances Bacterial Vaccines
    Language English
    Publishing date 2021-01-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 435191-5
    ISSN 1537-4521 ; 0148-5717
    ISSN (online) 1537-4521
    ISSN 0148-5717
    DOI 10.1097/OLQ.0000000000001331
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Phenotypic MicroArray Screening of Neisseria gonorrhoeae in Chemically Defined Liquid Medium.

    Baarda, Benjamin I / Sikora, Aleksandra E

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1997, Page(s) 207–216

    Abstract: Phenotype MicroArrays (PMs) provide a considerable benefit to the evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function. Nearly 2000 conditions can be screened relatively quickly either to search for phenotypes ... ...

    Abstract Phenotype MicroArrays (PMs) provide a considerable benefit to the evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function. Nearly 2000 conditions can be screened relatively quickly either to search for phenotypes associated with the loss of a protein or to understand metabolic differences between closely related bacterial isolates. The fastidious organism Neisseria gonorrhoeae presents an experimental challenge for phenotypic screening due to its nutrient restrictions and its autolytic activity upon reaching the stationary phase of growth. These limitations can be mitigated by modulating screening parameters. In this chapter, we describe a technique optimized for the phenotypic screening of N. gonorrhoeae FA1090 and isogenic mutant strains. Inoculum size and culturing times have been adjusted for growth in chemically defined, protein-free Graver-Wade liquid medium in the 96-well microtiter plate format employed by the PMs. With the conditions presented, highly reproducible gonococcal growth is achieved, and autolysis prior to the experimental endpoint is minimized.
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Culture Media/chemistry ; High-Throughput Screening Assays/methods ; Microarray Analysis/methods ; Mutation ; Neisseria gonorrhoeae/genetics ; Neisseria gonorrhoeae/metabolism ; Phenotype
    Chemical Substances Bacterial Proteins ; Culture Media
    Language English
    Publishing date 2019-05-23
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9496-0_13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Methods for Assessments of Collagenolytic Activity of the Vibrio cholerae Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin.

    Weber, Bo R / Sikora, Aleksandra E

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1839, Page(s) 153–169

    Abstract: Secreted proteases have been linked to facilitating the survival of Vibrio cholerae in different environmental niches. Examination of protease activity using various measures is critical to determine the substrate specificity of a given proteolytic ... ...

    Abstract Secreted proteases have been linked to facilitating the survival of Vibrio cholerae in different environmental niches. Examination of protease activity using various measures is critical to determine the substrate specificity of a given proteolytic enzyme and its biological function. Here, we describe methods to assess collagenolytic activity of the V. cholerae extracellular proteases using synthetic and natural substrates in quantitative and qualitative assays. Purification of the secreted V. cholerae collagenase VchC and extraction of type I collagen from fish skin are also described.
    MeSH term(s) Animals ; Cholera/microbiology ; Collagen Type I/metabolism ; Extracellular Space/metabolism ; Fishes ; Gelatin/metabolism ; Peptide Hydrolases/metabolism ; Proteolysis ; Skin/metabolism ; Substrate Specificity ; Swine ; Vibrio cholerae/physiology
    Chemical Substances Collagen Type I ; Gelatin (9000-70-8) ; Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2018-07-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8685-9_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Proteins secreted via the type II secretion system: smart strategies of Vibrio cholerae to maintain fitness in different ecological niches.

    Sikora, Aleksandra E

    PLoS pathogens

    2013  Volume 9, Issue 2, Page(s) e1003126

    MeSH term(s) Animals ; Aquatic Organisms ; Bacterial Proteins/metabolism ; Cholera/microbiology ; Cholera Toxin/metabolism ; Gene Expression Regulation, Bacterial ; Host-Pathogen Interactions ; Humans ; Substrate Specificity ; Vibrio cholerae/metabolism ; Vibrio cholerae/pathogenicity ; Vibrio cholerae/physiology ; Virulence Factors/metabolism
    Chemical Substances Bacterial Proteins ; Virulence Factors ; Cholera Toxin (9012-63-9)
    Language English
    Publishing date 2013-02-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1003126
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Comprehensive Bioinformatic Assessments of the Variability of Neisseria gonorrhoeae Vaccine Candidates.

    Baarda, Benjamin I / Zielke, Ryszard A / Holm, Alaina K / Sikora, Aleksandra E

    mSphere

    2021  Volume 6, Issue 1

    Abstract: A protective vaccine is the only viable way to stop the spread of gonorrhea in the face of rising antibiotic resistance. However, the notorious phase and antigenic variation ... ...

    Abstract A protective vaccine is the only viable way to stop the spread of gonorrhea in the face of rising antibiotic resistance. However, the notorious phase and antigenic variation of
    MeSH term(s) Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/genetics ; Computational Biology/methods ; Computational Biology/standards ; Gonorrhea/prevention & control ; Humans ; Neisseria gonorrhoeae/classification ; Neisseria gonorrhoeae/genetics ; Neisseria gonorrhoeae/immunology ; Neisseria meningitidis/genetics ; Phylogeny ; Polymorphism, Genetic
    Chemical Substances Antigens, Bacterial ; Bacterial Vaccines
    Language English
    Publishing date 2021-02-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.00977-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Development of a Tag/Catcher-mediated capsid virus-like particle vaccine presenting the conserved

    Martinez, Fabian G / Zielke, Ryszard A / Fougeroux, Cyrielle E / Li, Lixin / Sander, Adam F / Sikora, Aleksandra E

    Infection and immunity

    2023  Volume 91, Issue 12, Page(s) e0024523

    Abstract: Virus-like particles (VLPs) are promising nanotools for the development of subunit vaccines due to high immunogenicity and safety. Herein, we explored the versatile and effective Tag/Catcher-AP205 capsid VLP (cVLP) vaccine platform to address the urgent ... ...

    Abstract Virus-like particles (VLPs) are promising nanotools for the development of subunit vaccines due to high immunogenicity and safety. Herein, we explored the versatile and effective Tag/Catcher-AP205 capsid VLP (cVLP) vaccine platform to address the urgent need for the development of an effective and safe vaccine against gonorrhea. The benefits of this clinically validated cVLP platform include its ability to facilitate unidirectional, high-density display of complex/full-length antigens through an effective split-protein Tag/Catcher conjugation system. To assess this modular approach for making cVLP vaccines, we used a conserved surface lipoprotein, SliC, that contributes to the
    MeSH term(s) Animals ; Female ; Humans ; Mice ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Capsid ; Immunoglobulin A ; Immunoglobulin G ; Mice, Inbred BALB C ; Muramidase ; Neisseria gonorrhoeae/genetics ; Neisseria gonorrhoeae/immunology ; Vaccines, Virus-Like Particle/genetics ; Vaccines, Virus-Like Particle/immunology
    Chemical Substances Antigens, Bacterial ; Immunoglobulin A ; Immunoglobulin G ; Muramidase (EC 3.2.1.17) ; Vaccines, Virus-Like Particle
    Language English
    Publishing date 2023-11-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00245-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Infection: Proof of principle for effectiveness of a gonorrhoea vaccine.

    Unemo, Magnus / Sikora, Aleksandra E

    Nature reviews. Urology

    2017  Volume 14, Issue 11, Page(s) 643–644

    MeSH term(s) Case-Control Studies ; Gonorrhea ; Humans ; Meningococcal Vaccines ; Neisseria gonorrhoeae ; New Zealand ; Retrospective Studies
    Chemical Substances Meningococcal Vaccines
    Language English
    Publishing date 2017-08-31
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2493737-X
    ISSN 1759-4820 ; 1759-4812
    ISSN (online) 1759-4820
    ISSN 1759-4812
    DOI 10.1038/nrurol.2017.139
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  9. Article ; Online: Bioinformatics Workflow for Gonococcal Proteomics.

    El-Rami, Fadi E / Sikora, Aleksandra E

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1997, Page(s) 185–205

    Abstract: High-throughput quantitative proteomics unravels secrets of Neisseria gonorrhoeae biology by profiling proteome responses to environmental and endogenous cues and opens translational research paths through identification of vaccine candidates, drug ... ...

    Abstract High-throughput quantitative proteomics unravels secrets of Neisseria gonorrhoeae biology by profiling proteome responses to environmental and endogenous cues and opens translational research paths through identification of vaccine candidates, drug targets/virulence factors, and biomarkers. Bioinformatics tools and databases are indispensable for downstream analysis of proteomic datasets to generate biologically meaningful outcomes. In this chapter, we present a workflow for proteomic data analysis with emphasis on publicly available resources, software systems, and tools that predict protein subcellular localization (CELLO, PSORTb v3.0, SOSUI-GramN, SignalP 4.1, LipoP 1.0, TMHMM 2.0) and functional annotation (EggNOG-mapper 4.5.1., DAVID v6.8, and KEGG) of N. gonorrhoeae proteins. This computational step-by-step procedure may help to foster new hypotheses and to provide insights into the structure-function relationship of proteins.
    MeSH term(s) Bacterial Proteins/genetics ; Databases, Protein ; Datasets as Topic ; Gene Ontology ; Molecular Sequence Annotation ; Neisseria gonorrhoeae/genetics ; Proteome/genetics ; Proteomics/methods ; Software
    Chemical Substances Bacterial Proteins ; Proteome
    Language English
    Publishing date 2019-05-23
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9496-0_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Development and validation of multiplex assays for mouse and human IgG and IgA to Neisseria gonorrhoeae antigens.

    Stover, Erica L / Little, Marguerite B / Connolly, Kristie L / Li, Lixin / Nicholas, Robert A / Sikora, Aleksandra E / Jerse, Ann E / Hobbs, Marcia M / Duncan, J Alex / Macintyre, Andrew N

    The Journal of infectious diseases

    2024  

    Abstract: There is an urgent need for vaccines against Neisseria gonorrhoeae (Ng), the causative agent of gonorrhea. Vaccination with an outer-membrane vesicle (OMV)-based Neisseria meningitidis (Nm) vaccine provides some protection from Ng; however, the ... ...

    Abstract There is an urgent need for vaccines against Neisseria gonorrhoeae (Ng), the causative agent of gonorrhea. Vaccination with an outer-membrane vesicle (OMV)-based Neisseria meningitidis (Nm) vaccine provides some protection from Ng; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against Ng antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and inter-assay variability, and robustness to sample storage conditions. The assay was then used to test samples from mice and humans immunized with an Nm-OMV vaccine.
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiae153
    Database MEDical Literature Analysis and Retrieval System OnLINE

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