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  1. Article ; Online: Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF).

    Alteri, Claudia / Piermatteo, Lorenzo / Silberstein, Francesca Ceccherini / Svicher, Valentina / Perno, Carlo Federico

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2407, Page(s) 91–96

    Abstract: Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of ... ...

    Abstract Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the "hard-core" reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte-macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.
    MeSH term(s) Cells, Cultured ; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology ; HIV Infections/pathology ; HIV-1 ; Humans ; Macrophages/virology ; Monocytes/virology
    Chemical Substances Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
    Language English
    Publishing date 2022-01-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1871-4_8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Key mutations in the C-terminus of the HBV surface glycoprotein correlate with lower HBsAg levels

    Salpini, Romina / Battisti, Arianna / Piermatteo, Lorenzo / Carioti, Luca / Anastasiou, Olympia E / Gill, Upkar S / Di Carlo, Domenico / Colagrossi, Luna / Duca, Leonardo / Bertoli, Ada / La Rosa, Katia Yu / Fabeni, Lavinia / Iuvara, Alessandra / Malagnino, Vincenzo / Cerva, Carlotta / Lichtner, Miriam / Mastroianni, Claudio M / De Sanctis, Giuseppe Maria / Paoloni, Maurizio /
    Marignani, Massimo / Pasquazzi, Caterina / Iapadre, Nerio / Parruti, Giustino / Vecchiet, Jacopo / Sarmati, Loredana / Andreoni, Massimo / Angelico, Mario / Grelli, Sandro / T Kennedy, Patrick / Verheyen, Jens / Aquaro, Stefano / Silberstein, Francesca Ceccherini / Perno, Carlo Federico / Svicher, Valentina

    Emerging microbes & infections

    2020  Volume 9, Issue 1, Page(s) 928–939

    Abstract: Increasing evidences suggest that HBsAg-production varies across HBV-genotypes. HBsAg C-terminus plays a crucial role for HBsAg-secretion. Here, we evaluate HBsAg-levels in different HBV-genotypes in HBeAg-negative chronic infection, the correlation of ... ...

    Abstract Increasing evidences suggest that HBsAg-production varies across HBV-genotypes. HBsAg C-terminus plays a crucial role for HBsAg-secretion. Here, we evaluate HBsAg-levels in different HBV-genotypes in HBeAg-negative chronic infection, the correlation of specific mutations in HBsAg C-terminus with HBsAg-levels
    MeSH term(s) Adult ; Female ; Genotype ; Hepatitis B Surface Antigens/analysis ; Hepatitis B Surface Antigens/genetics ; Hepatitis B virus/genetics ; Hepatitis B virus/pathogenicity ; Hepatitis B, Chronic/diagnosis ; Humans ; Male ; Middle Aged ; Mutation
    Chemical Substances Hepatitis B Surface Antigens
    Language English
    Publishing date 2020-04-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2681359-2
    ISSN 2222-1751 ; 2222-1751
    ISSN (online) 2222-1751
    ISSN 2222-1751
    DOI 10.1080/22221751.2020.1757998
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin for patients with hepatitis C virus genotype 1 or 4 infection with cirrhosis (ABACUS): a prospective observational study.

    Petta, Salvatore / Marzioni, Marco / Russo, Pierluigi / Aghemo, Alessio / Alberti, Alfredo / Ascione, Antonio / Antinori, Andrea / Bruno, Raffaele / Bruno, Savino / Chirianni, Antonio / Gaeta, Giovanni Battista / Giannini, Edoardo G / Merli, Manuela / Messina, Vincenzo / Montilla, Simona / Perno, Carlo Federico / Puoti, Massimo / Raimondo, Giovanni / Rendina, Maria /
    Silberstein, Francesca Ceccherini / Villa, Erica / Zignego, Anna Linda / Pani, Luca / Craxì, Antonio

    The lancet. Gastroenterology & hepatology

    2017  Volume 2, Issue 6, Page(s) 427–434

    Abstract: Background: We ran a compassionate use nationwide programme (ABACUS) to provide access to ombitasvir, paritaprevir, and ritonavir, with dasabuvir, plus ribavirin for hepatitis C virus (HCV) genotype 1 infection and ombitasvir, paritaprevir, and ... ...

    Abstract Background: We ran a compassionate use nationwide programme (ABACUS) to provide access to ombitasvir, paritaprevir, and ritonavir, with dasabuvir, plus ribavirin for hepatitis C virus (HCV) genotype 1 infection and ombitasvir, paritaprevir, and ritonavir, plus ribavirin for HCV genotype 4 infection in patients with cirrhosis at high risk of decompensation while approval of these regimens was pending in Italy.
    Methods: In this prospective observational study, we collected data from a compassionate use nationwide programme from March 17, 2014, to May 28, 2015. Patients with HCV genotype 1 infection and cirrhosis at high risk of decompensation were given coformulated ombitasvir (25 mg), paritaprevir (150 mg), and ritonavir (100 mg) once daily and dasabuvir (250 mg) twice daily for 12 weeks (patients with HCV genotype 1b infection) or 24 weeks (patients with HCV genotype 1a infection). Patients with HCV genotype 4 infection were given coformulated ombitasvir (25 mg), paritaprevir (150 mg), and ritonavir (100 mg) once per day for 24 weeks. All patients were given weight-based ribavirin. The primary efficacy endpoint was sustained virological response at week 12 after the end of treatment (SVR12), analysed by intention-to-treat. Univariate and multivariate logistic regression analyses were used to identify baseline characteristics associated with SVR12. Adverse events were recorded throughout the study.
    Findings: 728 (96%) of 762 patients with cirrhosis who were given ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin therapy for 12 or 24 weeks achieved SVR12. Logistic regression analyses identified that bilirubin concentrations of less than 2 mg/dL were associated with SVR12 (odds ratio [OR] 4·76 [95% CI 1·83-12·3]; p=0·001). 166 (23%) of 734 patients included in safety analyses had an adverse event. 25 (3%) patients discontinued treatment because of adverse events. Asthenia was the most commonly reported adverse event, occurring in 36 (5%) patients.
    Interpretation: Our findings suggest that the safety and effectiveness of ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin in patients with HCV genotype 1 or 4 infection and cirrhosis at high risk of decompensation in a real-life setting are similar to those reported in clinical trials. The concordance with clinical trials provides reassurance that the reported efficacy of this treatment in clinical trials will translate to its use in routine clinical practice.
    Funding: Dipartimento Biomedico di Medicina Interna e Specialistica dell'Universita di Palermo.
    MeSH term(s) Adult ; Aged ; Anilides/adverse effects ; Anilides/therapeutic use ; Antiviral Agents/adverse effects ; Antiviral Agents/therapeutic use ; Carbamates/adverse effects ; Carbamates/therapeutic use ; Compassionate Use Trials ; Drug Therapy, Combination ; Female ; Genotype ; Hepatitis C, Chronic/complications ; Hepatitis C, Chronic/drug therapy ; Hepatitis C, Chronic/genetics ; Humans ; Liver Cirrhosis/complications ; Longitudinal Studies ; Macrocyclic Compounds/adverse effects ; Macrocyclic Compounds/therapeutic use ; Male ; Middle Aged ; Prospective Studies ; Ribavirin/adverse effects ; Ribavirin/therapeutic use ; Ritonavir/adverse effects ; Ritonavir/therapeutic use ; Sulfonamides/adverse effects ; Sulfonamides/therapeutic use ; Treatment Outcome ; Uracil/adverse effects ; Uracil/analogs & derivatives ; Uracil/therapeutic use
    Chemical Substances Anilides ; Antiviral Agents ; Carbamates ; Macrocyclic Compounds ; Sulfonamides ; ombitasvir (2302768XJ8) ; Ribavirin (49717AWG6K) ; Uracil (56HH86ZVCT) ; dasabuvir (DE54EQW8T1) ; Ritonavir (O3J8G9O825) ; paritaprevir (OU2YM37K86)
    Language English
    Publishing date 2017-04-10
    Publishing country Netherlands
    Document type Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
    ISSN 2468-1253
    ISSN (online) 2468-1253
    DOI 10.1016/S2468-1253(17)30048-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: results from the DREAM programme.

    Bellocchi, Maria Concetta / Forbici, Federica / Palombi, Leonardo / Gori, Caterina / Coelho, Elisabeth / Svicher, Valentina / D'Arrigo, Roberta / Emberti-Gialloreti, Leonardo / Ceffa, Susanna / Erba, Fulvio / Marazzi, Maria Cristina / Silberstein, Francesca Ceccherini / Perno, Carlo-Federico

    Journal of medical virology

    2005  Volume 76, Issue 4, Page(s) 452–458

    Abstract: Phylogenetic analysis and evaluation of drug-resistance were carried out upon 59 plasma samples from 58 treatment-naïve HIV-1 infected patients from Mozambique, enrolled in a free antiviral-therapy protocol in the frame of Drug-Resource-Enhancement ... ...

    Abstract Phylogenetic analysis and evaluation of drug-resistance were carried out upon 59 plasma samples from 58 treatment-naïve HIV-1 infected patients from Mozambique, enrolled in a free antiviral-therapy protocol in the frame of Drug-Resource-Enhancement against AIDS and Malnutrition (DREAM) programme. Sequencing of the first 1,300 bases of the pol-gene shows that all virus strains cluster within clade C, with the exception of a single patient carrying a G-subtype virus. Relevant mutations in the reverse transcriptase (RT) are rare: 118A/I/L/G (four patients), 179E/D/I (three patients), 333E/D (two patients), 101R, and 210F (one patient each). In Protease (PR), V82I (10.3%) is the only relevant mutation, while natural polymorphisms/secondary mutations are found, some at very high frequency: 20R (25.9%), 36I (91.4%), 36L (8.6%), 60E (31.0%), 63P (29.3%), and 93L (96.6%). Among them, mutations with a frequency >25% were further investigated to assess their covariation pattern with PI resistance associated mutations. The pattern of covariation observed for K20R and D60E (but not L63P and M36I) was different between C and B subtype isolates from PR-inhibitor-treated patients. The sequences were also analyzed to calculate the ratio of non-synonymous to synonymous substitution. The ratio for PR and RT was 0.116 and 0.093, respectively, suggesting a greater conservation in RT than PR in both subtypes B and C HIV strains. Taken together, the results demonstrate a consistent clade-homogeneity of viral strains circulating in Mozambique, and the very limited presence, in drug-naïve patients, of mutations associated with resistance to RT-inhibitors. The high frequency of secondary mutations/polymorphisms in HIV-PR deserves further studies to evaluate its relevance in clinical settings.
    MeSH term(s) Amino Acid Substitution/genetics ; Anti-HIV Agents/pharmacology ; Anti-HIV Agents/therapeutic use ; Drug Resistance, Viral/genetics ; Genes, pol ; Genotype ; HIV Infections/drug therapy ; HIV Infections/virology ; HIV Protease/genetics ; HIV Reverse Transcriptase/genetics ; HIV-1/classification ; HIV-1/drug effects ; HIV-1/genetics ; HIV-1/isolation & purification ; Humans ; Mozambique ; Mutation ; Phylogeny
    Chemical Substances Anti-HIV Agents ; HIV Reverse Transcriptase (EC 2.7.7.49) ; HIV Protease (EC 3.4.23.-)
    Language English
    Publishing date 2005-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.20382
    Database MEDical Literature Analysis and Retrieval System OnLINE

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