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  1. Article: Control of Enzymatic Browning in Strawberry, Apple, and Pear by Physical Food Preservation Methods: Comparing Ultrasound and High-Pressure Inactivation of Polyphenoloxidase.

    Silva, Filipa Vinagre Marques / Sulaiman, Alifdalino

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 13

    Abstract: Polyphenoloxidase (PPO) enzyme can be found in fruits, vegetables and crustaceans. Its activity, promoted by oxygen, causes food browning with subsequent loss of quality and limited shelf life. Foods are pasteurized with conventional and novel physical ... ...

    Abstract Polyphenoloxidase (PPO) enzyme can be found in fruits, vegetables and crustaceans. Its activity, promoted by oxygen, causes food browning with subsequent loss of quality and limited shelf life. Foods are pasteurized with conventional and novel physical methods to inactivate spoilage enzymes, thus avoiding the addition of unhealthy chemical preservatives. Ultrasound and high- pressure processing (HPP) are non-thermal technologies capable of retaining vitamins, bioactives and sensory components of fresh fruits. Enzyme residual activity vs. processing time were plotted for strawberry, apple, and pear purees subjected to thermosonication (1.3 W/g-71 °C), HPP-thermal (600 MPa-71 °C) and heat treatment alone at 71 °C. The PPO residual activities after treatments were highly variable. TS was the most effective for inactivating PPO, followed by thermal processing. HPP-thermal did not improve the inactivation compared with thermal treatment at 71 °C. The resistance of the three fruits' PPOs exhibited the same pattern for the three technologies: pear PPO was the most resistant enzyme, followed by apple PPO and, lastly, strawberry PPO. However, the resistance of the three PPOs to TS was lower and very similar. Given the huge variability of PPO resistance, it is important to run inactivation tests for different fruits/cultivars. The results can assist manufacturers to avoid browning during processing, storage and distribution of fruit purees, juices and concentrates.
    Language English
    Publishing date 2022-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11131942
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The Effect of Processing Methods on Food Quality and Human Health: Latest Advances and Prospects.

    Houška, Milan / Silva, Filipa Vinagre Marques

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 4

    Abstract: This Special Issue is focused on the use of modern food processing technologies to retain the highest possible content of health-promoting compounds in raw foods [ ... ]. ...

    Abstract This Special Issue is focused on the use of modern food processing technologies to retain the highest possible content of health-promoting compounds in raw foods [...].
    Language English
    Publishing date 2022-02-21
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11040611
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Ageing in the brain: mechanisms and rejuvenating strategies.

    Gaspar-Silva, Filipa / Trigo, Diogo / Magalhaes, Joana

    Cellular and molecular life sciences : CMLS

    2023  Volume 80, Issue 7, Page(s) 190

    Abstract: Ageing is characterized by the progressive loss of cellular homeostasis, leading to an overall decline of the organism's fitness. In the brain, ageing is highly associated with cognitive decline and neurodegenerative diseases. With the rise in life ... ...

    Abstract Ageing is characterized by the progressive loss of cellular homeostasis, leading to an overall decline of the organism's fitness. In the brain, ageing is highly associated with cognitive decline and neurodegenerative diseases. With the rise in life expectancy, characterizing the brain ageing process becomes fundamental for developing therapeutic interventions against the increased incidence of age-related neurodegenerative diseases and to aim for an increase in human life span and, more importantly, health span. In this review, we start by introducing the molecular/cellular hallmarks associated with brain ageing and their impact on brain cell populations. Subsequently, we assess emerging evidence on how systemic ageing translates into brain ageing. Finally, we revisit the mainstream and the novel rejuvenating strategies, discussing the most successful ones in delaying brain ageing and related diseases.
    MeSH term(s) Humans ; Aging ; Brain ; Longevity ; Neurodegenerative Diseases
    Language English
    Publishing date 2023-06-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-023-04832-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The Effect of Processing Methods on Food Quality and Human Health: Latest Advances and Prospects

    Houška, Milan / Silva, Filipa Vinagre Marques

    Foods. 2022 Feb. 21, v. 11, no. 4

    2022  

    Abstract: This Special Issue is focused on the use of modern food processing technologies to retain the highest possible content of health-promoting compounds in raw foods [ ... ] ...

    Abstract This Special Issue is focused on the use of modern food processing technologies to retain the highest possible content of health-promoting compounds in raw foods [...]
    Keywords food quality ; health promotion ; human health
    Language English
    Dates of publication 2022-0221
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11040611
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Control of Enzymatic Browning in Strawberry, Apple, and Pear by Physical Food Preservation Methods: Comparing Ultrasound and High-Pressure Inactivation of Polyphenoloxidase

    Silva, Filipa Vinagre Marques / Sulaiman, Alifdalino

    Foods. 2022 June 29, v. 11, no. 13

    2022  

    Abstract: Polyphenoloxidase (PPO) enzyme can be found in fruits, vegetables and crustaceans. Its activity, promoted by oxygen, causes food browning with subsequent loss of quality and limited shelf life. Foods are pasteurized with conventional and novel physical ... ...

    Abstract Polyphenoloxidase (PPO) enzyme can be found in fruits, vegetables and crustaceans. Its activity, promoted by oxygen, causes food browning with subsequent loss of quality and limited shelf life. Foods are pasteurized with conventional and novel physical methods to inactivate spoilage enzymes, thus avoiding the addition of unhealthy chemical preservatives. Ultrasound and high- pressure processing (HPP) are non-thermal technologies capable of retaining vitamins, bioactives and sensory components of fresh fruits. Enzyme residual activity vs. processing time were plotted for strawberry, apple, and pear purees subjected to thermosonication (1.3 W/g—71 °C), HPP-thermal (600 MPa—71 °C) and heat treatment alone at 71 °C. The PPO residual activities after treatments were highly variable. TS was the most effective for inactivating PPO, followed by thermal processing. HPP-thermal did not improve the inactivation compared with thermal treatment at 71 °C. The resistance of the three fruits’ PPOs exhibited the same pattern for the three technologies: pear PPO was the most resistant enzyme, followed by apple PPO and, lastly, strawberry PPO. However, the resistance of the three PPOs to TS was lower and very similar. Given the huge variability of PPO resistance, it is important to run inactivation tests for different fruits/cultivars. The results can assist manufacturers to avoid browning during processing, storage and distribution of fruit purees, juices and concentrates.
    Keywords apples ; bioactive compounds ; catechol oxidase ; cultivars ; fruits ; oxygen ; pears ; shelf life ; sonication ; spoilage ; strawberries ; ultrasonics
    Language English
    Dates of publication 2022-0629
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11131942
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Non-Thermal Processing of a Protein Functional Beverage Using Pulsed Electric Fields: Escherichia coli Inactivation and Effect on Proteins

    Alzahrani, Mona / Silva, Filipa V. M. / Alkhafaji, Sally

    Beverages. 2022 Nov. 01, v. 8, no. 4

    2022  

    Abstract: The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field ...

    Abstract The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field intensities were applied at different frequencies (400–900 Hz) to investigate the effect of different PEF conditions on the microbial population and proteins relevant to this functional beverage. The treatment temperature was kept below the lethal temperature of the microorganism under investigation. As power consumption plays an important role in the efficiency of the PEF application, the dissipated power was also estimated. Four log reductions in the E. coli population were obtained with 10 pulses at a 40 kV/cm field intensity and 25 pulses at a 25 kV/cm field intensity. PEF-treated whey-protein concentrates showed less denaturation in proteins than thermally treated concentrates, especially for lower electric field intensities (0% denaturation ± 0.007 at 25 kV/cm and 900 Hz, 4.41% denaturation ± 0.008 at 40 kV/cm and 400 Hz). Soy protein isolates manifested high sensitivity to PEF processing and resulted in denaturation and aggregation in the protein structure.
    Keywords Escherichia coli ; beverages ; denaturation ; electric field ; energy use and consumption ; protein structure ; temperature ; whey protein
    Language English
    Dates of publication 2022-1101
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2785444-9
    ISSN 2306-5710
    ISSN 2306-5710
    DOI 10.3390/beverages8040068
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Chemotherapy-free treatment of recurrent advanced ovarian cancer: myth or reality?

    Marques, Cristiana / Ferreira da Silva, Filipa / Sousa, Isabel / Nave, Mónica

    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

    2023  Volume 33, Issue 4, Page(s) 607–618

    Abstract: Advanced ovarian cancer remains a leading cause of death from gynecologic malignancy. Surgery and, in most cases, platinum-based chemotherapy with or without maintenance with bevacizumab and/or poly-ADP ribose polymerase inhibitors (PARPi) represent the ... ...

    Abstract Advanced ovarian cancer remains a leading cause of death from gynecologic malignancy. Surgery and, in most cases, platinum-based chemotherapy with or without maintenance with bevacizumab and/or poly-ADP ribose polymerase inhibitors (PARPi) represent the mainstay of treatment, but the disease typically recurs. The treatment of these patients represents a clinical challenge because sequential chemotherapy regimens are often used, with suboptimal outcomes and cumulative toxicity. Chemotherapy-free regimens, based on combinations of PARPi, vascular endothelial growth factor receptor inhibitors, anti-programmed cell death protein-1/programmed death-ligand 1, and anti-cytotoxic T-lymphocyte-associated protein-4 antibodies, among others, represent a valid option, with manageable toxicity profile and ease of administration. This review addresses this new strategy in the management of recurrent ovarian cancer and discusses its feasibility in the treatment landscape of the disease.
    MeSH term(s) Humans ; Female ; Ovarian Neoplasms/pathology ; Vascular Endothelial Growth Factor A ; Neoplasm Recurrence, Local/drug therapy ; Neoplasm Recurrence, Local/pathology ; Carcinoma, Ovarian Epithelial ; Bevacizumab
    Chemical Substances Vascular Endothelial Growth Factor A ; Bevacizumab (2S9ZZM9Q9V)
    Language English
    Publishing date 2023-04-03
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1070385-8
    ISSN 1525-1438 ; 1048-891X
    ISSN (online) 1525-1438
    ISSN 1048-891X
    DOI 10.1136/ijgc-2022-003719
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: From Therapy Resistance to Targeted Therapies in Prostate Cancer.

    Moreira-Silva, Filipa / Henrique, Rui / Jerónimo, Carmen

    Frontiers in oncology

    2022  Volume 12, Page(s) 877379

    Abstract: Prostate cancer (PCa) is the second most common malignancy among men worldwide. Although early-stage disease is curable, advanced stage PCa is mostly incurable and eventually becomes resistant to standard therapeutic options. Different genetic and ... ...

    Abstract Prostate cancer (PCa) is the second most common malignancy among men worldwide. Although early-stage disease is curable, advanced stage PCa is mostly incurable and eventually becomes resistant to standard therapeutic options. Different genetic and epigenetic alterations are associated with the development of therapy resistant PCa, with specific players being particularly involved in this process. Therefore, identification and targeting of these molecules with selective inhibitors might result in anti-tumoral effects. Herein, we describe the mechanisms underlying therapy resistance in PCa, focusing on the most relevant molecules, aiming to enlighten the current state of targeted therapies in PCa. We suggest that selective drug targeting, either alone or in combination with standard treatment options, might improve therapeutic sensitivity of resistant PCa. Moreover, an individualized analysis of tumor biology in each PCa patient might improve treatment selection and therapeutic response, enabling better disease management.
    Language English
    Publishing date 2022-05-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2022.877379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Ultrasound assisted thermal inactivation of spores in foods: Pathogenic and spoilage bacteria, molds and yeasts

    Evelyn / Silva, Filipa V.M

    Trends in food science & technology. 2020 Nov., v. 105

    2020  

    Abstract: Pasteurization aims to achieve the reduction of vegetative microorganisms and resistant spores which are of risk to public health. Power ultrasound has been identified as a potential technology for non-thermal food pasteurization. It relies on the ... ...

    Abstract Pasteurization aims to achieve the reduction of vegetative microorganisms and resistant spores which are of risk to public health. Power ultrasound has been identified as a potential technology for non-thermal food pasteurization. It relies on the propagation of pressure waves (frequency ranging from 20 to 100 kHz) of high intensity sound/acoustic energy (10–1000 W/cm²) inside a liquid or semisolid food, causing inactivation of pathogenic and spoilage microorganisms. This technology can also be used in simultaneous combination with temperature >80 °C (thermosonication, TS) to inactivate microbial spores.The current knowledge on the effect of ultrasound alone (US), TS, and ultrasound followed by a thermal process (US→T) on different type of spores relevant for low- and high-acidic foods was reviewed, including the kinetic models describing their inactivation in specific foods.US at room temperature (without heat) has no effect on microbial spores. In terms of microbial spore inactivation efficiency, TS was the best method, followed by US→T (non-simultaneous application) and finally heat treatment without US (T). TS employing temperatures between 90 °C and <100 °C is required for spore inactivation, as at the boiling point of water ultrasound intensity is reduced. There is a need to design efficient ultrasound probes which can withstand higher temperatures or incorporate pressure to improve gas bubble implosion, thus more results can be produced at higher temperatures and with different type of microorganisms. When reporting results from TS experiments, standardized ultrasound processing conditions including the actual power delivered to the food and expressed in acoustic power density (power/volume of liquid food, W/mL) or specific acoustic power (W/g food) should be used to allow the comparison of results from different studies. TS inactivation kinetics of some spores of bacteria and molds exhibited non-linear trends, thus TS should be examined in detail for reliable food processing.
    Keywords acoustics ; ambient temperature ; energy ; heat ; heat inactivation ; liquids ; pasteurization ; public health ; risk ; sonication ; spoilage ; spores ; ultrasonics
    Language English
    Dates of publication 2020-11
    Size p. 402-415.
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1049246-x
    ISSN 1879-3053 ; 0924-2244
    ISSN (online) 1879-3053
    ISSN 0924-2244
    DOI 10.1016/j.tifs.2020.09.020
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Resistant moulds as pasteurization target for cold distributed high pressure and heat assisted high pressure processed fruit products

    Silva, Filipa V.M / Evelyn

    Journal of food engineering. 2020 Oct., v. 282

    2020  

    Abstract: High pressure processing (HPP), also known as high hydrostatic pressure (HHP) is a modern method of food pasteurization used commercially in many countries. It relies on the application of very high pressures (up to 600 MPa) to the food/beverage to ... ...

    Abstract High pressure processing (HPP), also known as high hydrostatic pressure (HHP) is a modern method of food pasteurization used commercially in many countries. It relies on the application of very high pressures (up to 600 MPa) to the food/beverage to inactivate microorganisms. Since no heat or mild heat is applied, most of the original food sensory, nutrient and functional properties are retained after processing, and fresh-like fruit products with longer shelf-life are produced. In this study, a review of the resistance to HPP and HPTP (high pressure thermal process) of key bacteria, moulds and yeasts which often contaminate fruit products was undertaken. Spores of moulds Byssochlamys nivea - anamorph name Paecilomyces niveus or Neosartorya fischeri - anamorph name Aspergillus fischeri, are very resistant. A HPTP process of 600 MPa-75°C-15 min only caused a reduction of 1.4 log. Moulds are able to grow at temperatures between 10 and 43 °C, water activity between 0.892 and 0.992, over a wide range of pH (3–8), under reduced oxygen conditions inside food packs and in carbonated beverages, sometimes producing mycotoxins. Furthermore, HPP treated fruit products are cold stored, and therefore moulds can be an issue as they grow at temperatures as low as 10 °C. Therefore, in view of the acidity of fruit products, the high resistance to HPTP in particular older spores, the use of B. nivea or N. fischeri spores as reference microorganisms in the design of new HPP and HPTP processes with fruit products was proposed.
    Keywords Aspergillus fischeri ; Byssochlamys nivea ; Paecilomyces ; acidity ; anamorphs ; bacteria ; carbonated beverages ; cold ; cold storage ; fruit products ; functional properties ; heat ; high pressure treatment ; molds (fungi) ; mycotoxins ; oxygen ; pH ; pasteurization ; shelf life ; spores ; temperature ; water activity ; yeasts
    Language English
    Dates of publication 2020-10
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 622518-4
    ISSN 0260-8774
    ISSN 0260-8774
    DOI 10.1016/j.jfoodeng.2020.109998
    Database NAL-Catalogue (AGRICOLA)

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