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Article ; Online: Nanoimmunosensor for the electrochemical detection of oncostatin M receptor and monoclonal autoantibodies in systemic sclerosis.

Avelino, Karen Y P S / Silva-Junior, Alberto G / Pitta, Maira G R / Errachid, Abdelhamid / Oliveira, Maria D L / Andrade, César A S

Talanta

2023 Volume 256, Page(s) 124285

Abstract: Systemic sclerosis (SSc) is a chronic, autoimmune disease that primarily affects connective tissue. SSc can be classified into limited cutaneous (lSSc) and diffuse cutaneous (dSSc). Oncostatin M receptor (sOSMR) is an important inflammatory biomarker ... ...

Abstract	Systemic sclerosis (SSc) is a chronic, autoimmune disease that primarily affects connective tissue. SSc can be classified into limited cutaneous (lSSc) and diffuse cutaneous (dSSc). Oncostatin M receptor (sOSMR) is an important inflammatory biomarker expressed in the serum of patients with autoimmune diseases. A nanoengineered immunosensor surface was developed. The biosensor was composed of a conductive layer of polypyrrole, electrodeposited gold nanoparticles, and sOSMR protein for anti-human OSMR monoclonal antibody biorecognition. The electrochemical response evaluated by cyclic voltammetry and electrochemical impedance spectroscopy indicated the detection of the target analyte present in clinical samples from lSSc and dSSc patients. The voltammetric anodic shift for lSSc specimens was 82.7% ± 0.9-93.6% ± 3.2, and dSSc specimens was 118.7 ± 2.6 to 379.6 ± 2.6, revealing a differential diagnostic character for SSc subtypes. The sensor platform was adapted for identifying sOSMR, using anti-OSMR antibodies as bioreceptors. With a linear response range estimated from 0.005 to 500 pg mL
MeSH term(s)	Humans ; Autoantibodies ; Biomarkers ; Biosensing Techniques ; Gold ; Immunoassay ; Metal Nanoparticles ; Polymers ; Pyrroles ; Receptors, Oncostatin M ; Scleroderma, Systemic/diagnosis ; Electrochemical Techniques
Chemical Substances	Autoantibodies ; Biomarkers ; Gold (7440-57-5) ; Polymers ; Pyrroles ; Receptors, Oncostatin M
Language	English
Publishing date	2023-01-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.124285
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Article ; Online: Electrochemical detection of gram-negative bacteria through mastoparan-capped magnetic nanoparticle.

da Silva Junior, Alberto G / Frias, Isaac A M / Lima-Neto, Reginaldo G / Franco, Octávio L / Oliveira, Maria D L / Andrade, César A S

Enzyme and microbial technology

2022 Volume 160, Page(s) 110088

Abstract: The increasing number of multidrug resistance microorganisms is an alarming threat, and their rapid detection is essential to prevent nosocomial, foodborne, or waterborne infections. Many peptides derived from the venom of wasp Synoeca surinama have ... ...

Abstract	The increasing number of multidrug resistance microorganisms is an alarming threat, and their rapid detection is essential to prevent nosocomial, foodborne, or waterborne infections. Many peptides derived from the venom of wasp Synoeca surinama have antimicrobial activity against Gram-positive and Gram-negative bacteria. Synoeca-MP, an antimicrobial peptide (AMP) from mastoparan family, seems to increase bacterial membrane permeability, promoting cytotoxicity and membrane disruption. Here Synoeca-MP was evaluated as biorecognition element tethered over chitosan-coated magnetic nanoparticles (Fe
MeSH term(s)	Adenosine Monophosphate ; Anti-Bacterial Agents/pharmacology ; Biosensing Techniques/methods ; Electrochemical Techniques/methods ; Gold/chemistry ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Intercellular Signaling Peptides and Proteins ; Magnetite Nanoparticles/chemistry ; Wasp Venoms
Chemical Substances	Anti-Bacterial Agents ; Intercellular Signaling Peptides and Proteins ; Magnetite Nanoparticles ; Wasp Venoms ; Adenosine Monophosphate (415SHH325A) ; mastoparan (72093-21-1) ; Gold (7440-57-5)
Language	English
Publishing date	2022-06-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	423729-8
ISSN	1879-0909 ; 0141-0229
ISSN (online)	1879-0909
ISSN	0141-0229
DOI	10.1016/j.enzmictec.2022.110088
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Article: Electrochemical detection of gram-negative bacteria through mastoparan-capped magnetic nanoparticle

da Silva Junior, Alberto G. / Frias, Isaac A.M. / Lima-Neto, Reginaldo G. / Franco, Octávio L. / Oliveira, Maria D.L. / Andrade, César A.S.

Enzyme and microbial technology. 2022 June 16,

2022

Abstract	The increasing number of multidrug resistance microorganisms is an alarming threat, and their rapid detection is essential to prevent nosocomial, foodborne, or waterborne infections. Many peptides derived from the venom of wasp Synoeca surinama have antimicrobial activity against Gram-positive and Gram-negative bacteria. Synoeca-MP, an antimicrobial peptide (AMP) from mastoparan family, seems to increase bacterial membrane permeability, promoting cytotoxicity and membrane disruption. Here Synoeca-MP was evaluated as biorecognition element tethered over chitosan-coated magnetic nanoparticles (Fe₃O₄-Chit). The transducing layer of the biosensor was developed from the self-assembling of 4-mercaptobenzoic acid (4-MBA) monolayer onto gold substrate. Atomic force microscopy (AFM) analyses confirmed the biointeraction between AMP and different pathogens membranes. The fabrication and performance of the biosensing assembly were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Detection of Enterococcus faecalis (G+), Klebsiella pneumoniae (G-), Pseudomonas aeruginosa (G-), and Candida tropicalis was assessed in a recognition range from 10¹ to 10⁵ CFU.mL⁻¹. An instrumental limit of detection of 10 CFU.mL⁻¹ was obtained for each specimen. However, the device presented a preferential selectivity towards Gram-negative bacteria. The proposed biosensor is a sensitive, fast, and straightforward platform for microbial detection in aqueous samples, envisaged for environmental monitoring applications.
Keywords	Candida tropicalis ; Enterococcus faecalis ; Klebsiella pneumoniae ; Pseudomonas aeruginosa ; antimicrobial peptides ; antimicrobial properties ; atomic force microscopy ; biosensors ; cross infection ; cytotoxicity ; detection limit ; dielectric spectroscopy ; electrochemistry ; enzymes ; gold ; magnetism ; membrane permeability ; microbial detection ; multiple drug resistance ; rapid methods ; venoms ; voltammetry ; wasps
Language	English
Dates of publication	2022-0616
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	423729-8
ISSN	1879-0909 ; 0141-0229
ISSN (online)	1879-0909
ISSN	0141-0229
DOI	10.1016/j.enzmictec.2022.110088
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Article ; Online: Concanavalin A differentiates gram-positive bacteria through hierarchized nanostructured transducer.

da Silva Junior, Alberto G / Frias, Isaac A M / Lima-Neto, Reginaldo G / Sá, Sandra R / Oliveira, Maria D L / Andrade, César A S

Microbiological research

2021 Volume 251, Page(s) 126834

Abstract: Biosensors are pre-prepared diagnostic devices composed of at least one biological probe. These devices are envisaged for the practical identification of specific targets of microbiological interest. In recent years, the use of narrow-specific probes ... ...

Abstract	Biosensors are pre-prepared diagnostic devices composed of at least one biological probe. These devices are envisaged for the practical identification of specific targets of microbiological interest. In recent years, the use of narrow-specific probes such as lectins has been proven to distinguish bacteria and glycoproteins based on their superficial glycomic pattern. For instance, Concanavalin A is a carbohydrate-binding lectin indicated as a narrow-specific biological probe for Gram-negative bacteria. As a drawback, Gram-positive bacteria are frequently overlooked from lectin-based biosensing studies because their identification results in low resolution and overlapped signals. In this work, the authors explore the effect that platform nanostructuration has over the electrochemical response of ConA-based platforms constructed for bacterial detection; one is formed of chitosan-capped magnetic nanoparticles, and another is composed of gold nanoparticle-decorated magnetic nanoparticles. The biosensing platforms were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) as a function of bacterial concentration. Our results show that probe-target interaction causes variations in the electrical responses of nanostructured transducers. Moreover, the association of gold nanoparticles to magnetic nanoparticles resulted in an electrical enhancement capable of overcoming low resolution and overlapping Gram-positive identification. Both platforms attained a limit of detection of 10 ° CFU mL
MeSH term(s)	Biosensing Techniques/methods ; Concanavalin A/pharmacology ; Gold ; Gram-Positive Bacteria/classification ; Gram-Positive Bacteria/drug effects ; Metal Nanoparticles ; Transducers
Chemical Substances	Concanavalin A (11028-71-0) ; Gold (7440-57-5)
Language	English
Publishing date	2021-07-31
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1189614-0
ISSN	1618-0623 ; 0944-5013
ISSN (online)	1618-0623
ISSN	0944-5013
DOI	10.1016/j.micres.2021.126834
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Article: Concanavalin A differentiates gram-positive bacteria through hierarchized nanostructured transducer

da Silva Junior, Alberto G. / Frias, Isaac A.M. / Lima-Neto, Reginaldo G. / Sá, Sandra R. / Oliveira, Maria D.L. / Andrade, César A.S.

Microbiological research. 2021 Oct., v. 251

2021

Abstract	Biosensors are pre-prepared diagnostic devices composed of at least one biological probe. These devices are envisaged for the practical identification of specific targets of microbiological interest. In recent years, the use of narrow-specific probes such as lectins has been proven to distinguish bacteria and glycoproteins based on their superficial glycomic pattern. For instance, Concanavalin A is a carbohydrate-binding lectin indicated as a narrow-specific biological probe for Gram-negative bacteria. As a drawback, Gram-positive bacteria are frequently overlooked from lectin-based biosensing studies because their identification results in low resolution and overlapped signals. In this work, the authors explore the effect that platform nanostructuration has over the electrochemical response of ConA-based platforms constructed for bacterial detection; one is formed of chitosan-capped magnetic nanoparticles, and another is composed of gold nanoparticle-decorated magnetic nanoparticles. The biosensing platforms were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) as a function of bacterial concentration. Our results show that probe-target interaction causes variations in the electrical responses of nanostructured transducers. Moreover, the association of gold nanoparticles to magnetic nanoparticles resulted in an electrical enhancement capable of overcoming low resolution and overlapping Gram-positive identification. Both platforms attained a limit of detection of 10 ° CFU mL⁻¹, which is useful for water analyses and sanitation concerns, where low CFU mL⁻¹ are always expected. Although both platforms were able to detect Gram-negative bacteria, Gram-positives were only correctly differentiated by the gold nanoparticle-decorated magnetic nanoparticles, thus demonstrating the positive influence of hierarchically nanostructured platforms.
Keywords	biosensors ; carbohydrate binding ; concanavalin A ; detection limit ; dielectric spectroscopy ; electrochemistry ; glycomics ; glycoproteins ; gold ; magnetism ; microbial detection ; nanogold ; research ; sanitation ; voltammetry
Language	English
Dates of publication	2021-10
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1189614-0
ISSN	1618-0623 ; 0944-5013
ISSN (online)	1618-0623
ISSN	0944-5013
DOI	10.1016/j.micres.2021.126834
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Article ; Online: Lectin-based impedimetric biosensor for differentiation of pathogenic candida species.

Sá, Sandra R / Silva Junior, Alberto G / Lima-Neto, Reginaldo G / Andrade, Cesar A S / Oliveira, Maria D L

Talanta

2020 Volume 220, Page(s) 121375

Abstract: Fungi stand out as primary pathogens present in healthcare-acquired infections, presenting an increased number of cases even using appropriate antifungal therapy. Candida spp. is a predominant microorganism among several fungal pathogens present in the ... ...

Abstract	Fungi stand out as primary pathogens present in healthcare-acquired infections, presenting an increased number of cases even using appropriate antifungal therapy. Candida spp. is a predominant microorganism among several fungal pathogens present in the healthcare setting. Candidemia and candidiasis are fungal infections responsible for high morbidity and mortality among ill patients in hospitals. It is noticeable that prolonged hospital stays lead to a higher economic impact and increased risk for developing secondary fungal or even bacterial infections. New fast and sensitive approaches for the detection of Candida species is highly required. Electrochemical biosensors are an excellent alternative to conventional techniques by combining fast analyte detection, low cost, and the possibility of miniaturization. Lectins are carbohydrate-binding proteins with the capability to reach out to the microorganism cell wall. In this work, we proposed the development of an impedimetric biosensor for Candida spp. based on Concanavalin A (ConA) and wheat germ agglutinin (WGA) as recognition agents of the yeast cells. Atomic force microscopy images indicate changes in the biosensor surface after assembly of the molecules and exposure to fungal samples. Electrochemical impedance spectroscopy results revealed a proportional increase of charge transfer resistance (R
MeSH term(s)	Antifungal Agents ; Biosensing Techniques ; Candida ; Cell Differentiation ; Humans ; Lectins ; Microbial Sensitivity Tests ; Pichia
Chemical Substances	Antifungal Agents ; Lectins
Language	English
Publishing date	2020-07-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2020.121375
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Article: Lectin-based impedimetric biosensor for differentiation of pathogenic candida species

Sá, Sandra R / Silva Junior, Alberto G / Lima-Neto, Reginaldo G / Andrade, Cesar A.S / Oliveira, Maria D.L

Talanta. 2020 Dec. 01, v. 220

2020

Abstract	Fungi stand out as primary pathogens present in healthcare-acquired infections, presenting an increased number of cases even using appropriate antifungal therapy. Candida spp. is a predominant microorganism among several fungal pathogens present in the healthcare setting. Candidemia and candidiasis are fungal infections responsible for high morbidity and mortality among ill patients in hospitals. It is noticeable that prolonged hospital stays lead to a higher economic impact and increased risk for developing secondary fungal or even bacterial infections. New fast and sensitive approaches for the detection of Candida species is highly required. Electrochemical biosensors are an excellent alternative to conventional techniques by combining fast analyte detection, low cost, and the possibility of miniaturization. Lectins are carbohydrate-binding proteins with the capability to reach out to the microorganism cell wall. In this work, we proposed the development of an impedimetric biosensor for Candida spp. based on Concanavalin A (ConA) and wheat germ agglutinin (WGA) as recognition agents of the yeast cells. Atomic force microscopy images indicate changes in the biosensor surface after assembly of the molecules and exposure to fungal samples. Electrochemical impedance spectroscopy results revealed a proportional increase of charge transfer resistance (RCT) as fungal CFU increased, where four Candida species were evaluated (Candida krusei, Candida tropicalis, Candida parapsilosis and Candida albicans). The biosensor is useful to differentiate Candida spp. with a detection limit between 10² to 10⁶ CFU mL⁻¹. The obtained biosensor appears as an innovative candidate for the detection and differentiation of pathogenic Candida spp.
Keywords	Candida albicans ; Candida krusei ; Candida parapsilosis ; Candida tropicalis ; agglutinins ; atomic force microscopy ; bacterial infections ; biosensors ; candidiasis ; carbohydrate binding ; cell walls ; concanavalin A ; detection limit ; dielectric spectroscopy ; economic impact ; health services ; hospitals ; morbidity ; mortality ; pathogens ; patients ; therapeutics ; wheat germ ; yeasts
Language	English
Dates of publication	2020-1201
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2020.121375
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Article ; Online: Nanostructured sensor platform based on organic polymer conjugated to metallic nanoparticle for the impedimetric detection of SARS-CoV-2 at various stages of viral infection.

Avelino, Karen Y P S / Dos Santos, Giselle S / Frías, Isaac A M / Silva-Junior, Alberto G / Pereira, Michelly C / Pitta, Maira G R / de Araújo, Breno C / Errachid, Abdelhamid / Oliveira, Maria D L / Andrade, César A S

Journal of pharmaceutical and biomedical analysis

2021 Volume 206, Page(s) 114392

Abstract: The projection of new biosensing technologies for genetic identification of SARS-COV-2 is essential in the face of a pandemic scenario. For this reason, the current research aims to develop a label-free flexible biodevice applicable to COVID-19. A ... ...

Abstract	The projection of new biosensing technologies for genetic identification of SARS-COV-2 is essential in the face of a pandemic scenario. For this reason, the current research aims to develop a label-free flexible biodevice applicable to COVID-19. A nanostructured platform made of polypyrrole (PPy) and gold nanoparticles (GNP) was designed for interfacing the electrochemical signal in miniaturized electrodes of tin-doped indium oxide (ITO). Oligonucleotide primer was chemically immobilized on the flexible transducers for the biorecognition of the nucleocapsid protein (N) gene. Methodological protocols based on cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM) were used to characterize the nanotechnological apparatus. The biosensor's electrochemical performance was evaluated using the SARS-CoV-2 genome and biological samples of cDNA from patients infected with retrovirus at various disease stages. It is inferred that the analytical tool was able to distinguish the expression of SARS-CoV-2 in patients diagnosed with COVID-19 in the early, intermediate and late stages. The biosensor exhibited high selectivity by not recognizing the biological target in samples from patients not infected with SARS-CoV-2. The proposed sensor obtained a linear response range estimated from 800 to 4000 copies µL
MeSH term(s)	Antibodies, Immobilized ; Biosensing Techniques ; COVID-19 ; Electrochemical Techniques ; Electrodes ; Gold ; Humans ; Limit of Detection ; Metal Nanoparticles ; Microelectrodes ; Polymers ; Pyrroles ; SARS-CoV-2
Chemical Substances	Antibodies, Immobilized ; Polymers ; Pyrroles ; Gold (7440-57-5)
Language	English
Publishing date	2021-09-24
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2021.114392
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