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Article ; Online: Interventional Magnetic Resonance Imaging Suite (IMRIS): How to build and how to use.

Stefanini, Matteo / Simonetti, Giovanni

2022 Volume 127, Issue 10, Page(s) 1063–1067

Abstract: Over the last ten years, the number of minimally invasive interventional procedures has been increasing steadily. Magnetic resonance imaging (MRI) is still far less frequently used for image-guided percutaneous procedures or as an intervention method ... ...

Abstract	Over the last ten years, the number of minimally invasive interventional procedures has been increasing steadily. Magnetic resonance imaging (MRI) is still far less frequently used for image-guided percutaneous procedures or as an intervention method than computed tomography (CT) and ultrasound (US). However, MRI has many advantages, such as lack of ionizing radiation, real-time MR-fluoroscopy placement high resolution, no bone and gas artifacts, the ability to display lesions that are difficult to observe on CT and US visualization of blood vessels without a contrast agent, free selection of imaging planes and in the case of procedures such as thermos or cryoablation it is possible to make an intraprocedural assessment of the process without the application of a contrasting agent (Marini et al. in Diagn Interv Imaging 102: 531-538, 2021; Li et al. in BMC Cancer 21: 366, 2021; Barkhausen et al. in Rofo 189: 611, 2017). Furthermore, in addition to its use in Interventional Radiology, MRI is also becoming a reality in cardiology thanks to the possibility of overcoming all the limits related to the fusion technology used up to now in cardiac ablations (Chubb et al. Arrhythm Electrophysiol Rev 6: 85, 2017). Some suites have been built in Northern Europe and in the United States, but based only on personal experience and industry indication. In our hospital, we have built a new Interventional Magnetic Resonance Imaging Suite (IMRIS), the first in Southern Europe and we have defined what the necessary requirements and equipment were for the safety of the patient and the operator.
MeSH term(s)	Contrast Media ; Fluoroscopy ; Humans ; Magnetic Resonance Imaging/methods ; Magnetic Resonance Imaging, Interventional/methods ; Tomography, X-Ray Computed
Chemical Substances	Contrast Media
Language	English
Publishing date	2022-08-26
Publishing country	Italy
Document type	Editorial
ZDB-ID	205751-7
ISSN	1826-6983 ; 0033-8362
ISSN (online)	1826-6983
ISSN	0033-8362
DOI	10.1007/s11547-022-01537-x
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Article: Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of

Bonincontro, Graziana / Scuderi, Sarah Adriana / Marino, Andreana / Simonetti, Giovanna

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 11

Abstract: Bacterial and fungal biofilm has increased antibiotic resistance and plays an essential role in many persistent diseases. Biofilm-associated chronic infections are difficult to treat and reduce the efficacy of medical devices. This global problem has ... ...

Abstract	Bacterial and fungal biofilm has increased antibiotic resistance and plays an essential role in many persistent diseases. Biofilm-associated chronic infections are difficult to treat and reduce the efficacy of medical devices. This global problem has prompted extensive research to find alternative strategies to fight microbial chronic infections. Plant bioactive metabolites with antibiofilm activity are known to be potential resources to alleviate this problem. The phytochemical screening of some medicinal plants showed different active groups, such as stilbenes, tannins, alkaloids, terpenes, polyphenolics, flavonoids, lignans, quinones, and coumarins. Synergistic effects can be observed in the interaction between plant compounds and conventional drugs. This review analyses and summarises the current knowledge on the synergistic effects of plant metabolites in combination with conventional antimicrobials against biofilms of
Language	English
Publishing date	2023-10-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16111531
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Article ; Online: The role of body mass index in the development of thromboembolic events among cancer patients with PICCs: a systematic review.

Simonetti, G / Bersani, A / Tramacere, I / Lusignani, M / Gaviani, P / Silvani, A

Journal of vascular nursing : official publication of the Society for Peripheral Vascular Nursing

2021 Volume 40, Issue 1, Page(s) 11–16

Abstract: Objective: Peripherally inserted central catheters (PICC) guarantee a stable and safe vascular access to administer irritants or vesicants therapies. However, they may occasionally be affected by relevant thrombotic complications especially in patients ... ...

Abstract	Objective: Peripherally inserted central catheters (PICC) guarantee a stable and safe vascular access to administer irritants or vesicants therapies. However, they may occasionally be affected by relevant thrombotic complications especially in patients with hypercoagulability such as oncological patients. Among the identification of independent risk factors, the role of body mass index (BMI) ≥25 kg/m Data sources and review methods: A scientific literature review was performed in Pubmed, Embase and Cinahl from Jan 1, 2010 to September 10, 2020 in which we identified 100 records. Of these, 88 were excluded and 14 were reviewed in full text. Among the reviewed records, 6 articles satisfied the inclusion criteria for analysis. These criteria included the English language, oncological patients with PICCs, the evaluation of catheter-related thrombosis as well as the stratification of patients according to BMI. Studies off topic and lacking data on PICC related complications among overweight and underweight patients were excluded. The includedstudies, judged with Newcastle-Ottawa Scale, was fair-lower quality. The primary endpoint was the relative risk (RR) of PICC-related thrombosis of overweight/obese vs normal weight/underweight (i.e., BMI ≥25 vs <25 kg/m Results: A total of 2431 patients were included in the analysis. Overall, 15.1% of patients developed PICC-related thrombosis within a median time of 23.2 days (range 11.0-42.5) after PICC implantation. Concerning BMI, 52.6% of the entire population was overweight/obese. We assessed the proportion of patients with PICC-related thrombotic events in the two groups, with 28% (95% CI, 12%-45%) of events registered in the overweight/obese patients cohort, and 13% (95% CI, 6%-19%) in the normal weight/underweight cohort. The pooled relative risk (RR) was 2.06 (95% CI, 1.21-3.49, p<0.001) in overweight/obese vs normal weight/underweight patients. Conclusion: This review showed a two-fold risk of thrombosis in overweight/obese compared to normal weight/underweight oncological patients with PICCs. Underweight condition could also play a role in thrombosis development, especially in nasopharyngeal and digestive system cancer. Future prospective studies are needed to achieve reliable results and produce useful conclusion.
MeSH term(s)	Body Mass Index ; Catheterization, Central Venous/adverse effects ; Catheterization, Peripheral/adverse effects ; Humans ; Neoplasms/complications ; Obesity/complications ; Overweight/etiology ; Retrospective Studies ; Risk Factors ; Thinness/etiology ; Thromboembolism/etiology
Language	English
Publishing date	2021-10-26
Publishing country	United States
Document type	Journal Article ; Systematic Review
ZDB-ID	1083367-5
ISSN	1532-6578 ; 1062-0303
ISSN (online)	1532-6578
ISSN	1062-0303
DOI	10.1016/j.jvn.2021.10.001
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Article ; Online: Anatomical Bases of the Temporal Muscle Trigger Points.

Garrido, Luis Carlos Fernandez / Simonetti, Giulianna / Saleh, Samir Omar / Hojaij, Flávio / Andrade, Mauro / Jacomo, Alfredo Luiz / Akamatsu, Flavia Emi

BioMed research international

2024 Volume 2024, Page(s) 6641346

Abstract: Method: Temporal muscles of 14 adult cadavers were studied. The muscle bellies were divided into six areas, three superior (1.2 and 3) and three inferior areas (4, 5, and 6) lower, according to a Cartesian plane to analyze and describe the entry points ... ...

Abstract	Method: Temporal muscles of 14 adult cadavers were studied. The muscle bellies were divided into six areas, three superior (1.2 and 3) and three inferior areas (4, 5, and 6) lower, according to a Cartesian plane to analyze and describe the entry points of the branches of the deep temporal nerves into the muscle. The branching distribution was analyzed using Poisson log-linear tests with Bonferroni post hoc tests for comparison between groups (sextants) ( Results: Deep temporal nerve entry points were found in the temporal muscle in all areas. Most of the branches were observed in areas 2 and 5, which coincide with the muscle fibers responsible for mandible elevation and related to the previously described MTPs. Fewer branches were found in areas 1 and 6, where contraction produces mandible retraction. Conclusion: There is an anatomical correlation between the branching pattern of the deep temporal nerve and temporal muscle trigger points. Adequate knowledge of the innervation of the temporal muscle may help elucidate the pathophysiology of myofascial syndromes and provide a rational basis for interventional or conservative approaches and help surgeons avoid iatrogenic lesions to the deep temporal nerve lesion.
MeSH term(s)	Adult ; Humans ; Temporal Muscle ; Trigger Points ; Cadaver ; Mandible ; Muscle Fibers, Skeletal
Language	English
Publishing date	2024-02-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2024/6641346
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Article: Studies of Potential Migration of Hazardous Chemicals from Sustainable Food Contact Materials.

Simonetti, Giulia / Riccardi, Carmela / Pomata, Donatella / Acquaviva, Luca / Fricano, Andrea / Buiarelli, Francesca / Senofonte, Marta / Di Filippo, Patrizia

Foods (Basel, Switzerland)

2024 Volume 13, Issue 5

Abstract: In recent years, due to modern techniques for the distribution, transport, and retail sale of food, the production of large amounts of non-biodegradable and bioaccumulative packaging waste has become a major environmental issue. To address this issue, ... ...

Abstract	In recent years, due to modern techniques for the distribution, transport, and retail sale of food, the production of large amounts of non-biodegradable and bioaccumulative packaging waste has become a major environmental issue. To address this issue, new food packaging materials based on renewable biomass have been studied as eco-friendly, biodegradable, and biocompatible alternatives to synthetic materials. However, although these materials are not petrochemical derivatives, the presence of contaminants cannot be excluded. This work aims to extend the knowledge on bio-based packaging materials, researching the presence of contaminants potentially able to migrate to food at concentrations of concern. In this study, we focus on two classes of contaminants, organophosphate esters (OPEs) and perfluoroalkyl substances (PFASs), carrying out migration tests toward different simulants, according to the current European regulation. PFAS analysis was performed using high-resolution liquid chromatography coupled to ion trap-tandem mass spectrometry (QTrap). OPE analyses were performed both by gas chromatography-mass spectrometry (GC-MS) and high-resolution liquid chromatography coupled to triple quadrupole mass spectrometry (TQMS). Preliminary findings demonstrate the release of toxic OPEs and PFASs from bio-based food packaging, highlighting the need to investigate the presence of potentially harmful chemicals in these materials.
Language	English
Publishing date	2024-02-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods13050645
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Article ; Online: Targeting Proliferation Signals and the Cell Cycle Machinery in Acute Leukemias: Novel Molecules on the Horizon.

Ghelli Luserna di Rorà, Andrea / Jandoubi, Mouna / Martinelli, Giovanni / Simonetti, Giorgia

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 3

Abstract: Uncontrolled proliferative signals and cell cycle dysregulation due to genomic or functional alterations are important drivers of the expansion of undifferentiated blast cells in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) cells. ... ...

Abstract	Uncontrolled proliferative signals and cell cycle dysregulation due to genomic or functional alterations are important drivers of the expansion of undifferentiated blast cells in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) cells. Therefore, they are largely studied as potential therapeutic targets in the field. We here present the most recent advancements in the evaluation of novel compounds targeting cell cycle proteins or oncogenic mechanisms, including those showing an antiproliferative effect in acute leukemia, independently of the identification of a specific target. Several new kinase inhibitors have been synthesized that showed effectiveness in a nanomolar to micromolar concentration range as inhibitors of FLT3 and its mutant forms, a highly attractive therapeutic target due to its driver role in a significant fraction of AML cases. Moreover, we introduce novel molecules functioning as microtubule-depolymerizing or P53-restoring agents, G-quadruplex-stabilizing molecules and CDK2, CHK1, PI3Kδ, STAT5, BRD4 and BRPF1 inhibitors. We here discuss their mechanisms of action, including the downstream intracellular changes induced by in vitro treatment, hematopoietic toxicity, in vivo bio-availability and efficacy in murine xenograft models. The promising activity profile demonstrated by some of these candidates deserves further development towards clinical investigation.
MeSH term(s)	Humans ; Animals ; Mice ; Nuclear Proteins/metabolism ; Transcription Factors/metabolism ; Leukemia, Myeloid, Acute/metabolism ; Acute Disease ; Cell Cycle ; Cell Division ; Protein Kinase Inhibitors/pharmacology ; Protein Kinase Inhibitors/therapeutic use ; Cell Proliferation ; Cell Line, Tumor ; Apoptosis ; DNA-Binding Proteins/metabolism ; Adaptor Proteins, Signal Transducing/metabolism ; Cell Cycle Proteins/metabolism
Chemical Substances	Nuclear Proteins ; Transcription Factors ; Protein Kinase Inhibitors ; BRPF1 protein, human ; DNA-Binding Proteins ; Adaptor Proteins, Signal Transducing ; BRD4 protein, human ; Cell Cycle Proteins
Language	English
Publishing date	2023-01-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28031224
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Article ; Online: Occurrence and congener profiles of dioxins (PCDDs), furans (PCDFs) and polychlorinated biphenyls (PCBs) in ovine and caprine milk samples collected in a very polluted site in Central Italy.

Castellani, Federica / Marini, Federico / Simonetti, Giulia / Protano, Carmela / Fabiani, Leila / Manzoli, Lamberto / Vitali, Matteo

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

2023 Volume 40, Issue 3, Page(s) 415–424

Abstract: Concentrations of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 18 polychlorinated biphenyls (PCBs), including 12 dioxin-like (DL-PCBs) and 6 non-dioxin-like PCBs (NDL-PCBs) were measured in 16 ovine and ... ...

Abstract	Concentrations of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 18 polychlorinated biphenyls (PCBs), including 12 dioxin-like (DL-PCBs) and 6 non-dioxin-like PCBs (NDL-PCBs) were measured in 16 ovine and caprine milk samples collected in the territory of Bussi sul Tirino, central Italy, a Site of National Interest (SNI) due to its high and widespread environmental pollution. All the analyzed samples were compliant with the maximum levels fixed by Commission Regulation (EU) 1259/2011 for the content of PCDD/Fs and the sum of PCDD/Fs and DL-PCBs. In two cases, contamination levels of the sum of PCDD/Fs and DL-PCBs were higher than the action levels fixed by EU Recommendation 663/2014. The statistical analysis, performed by Principal Component Analysis (PCA), revealed that the differences in contamination profiles of the different milk samples were independent of the distance of the farms from the Bussi illegal landfill but likely related to local emission sources influencing the exposure to POPs of studied animals.
MeSH term(s)	Animals ; Sheep ; Polychlorinated Dibenzodioxins/analysis ; Polychlorinated Biphenyls/analysis ; Dioxins/analysis ; Dibenzofurans, Polychlorinated/analysis ; Milk/chemistry ; Dibenzofurans/analysis ; Goats ; Furans/analysis ; Benzofurans/analysis ; Sheep, Domestic ; Italy
Chemical Substances	Polychlorinated Dibenzodioxins ; Polychlorinated Biphenyls (DFC2HB4I0K) ; Dioxins ; Dibenzofurans, Polychlorinated ; Dibenzofurans ; Furans ; Benzofurans
Language	English
Publishing date	2023-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2462951-0
ISSN	1944-0057 ; 1944-0049
ISSN (online)	1944-0057
ISSN	1944-0049
DOI	10.1080/19440049.2023.2173811
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Article ; Online: Germline

Bochicchio, Maria Teresa / Micucci, Giorgia / Asioli, Silvia / Ghetti, Martina / Simonetti, Giorgia / Lucchesi, Alessandro

International journal of molecular sciences

2023 Volume 24, Issue 22

Abstract: Chronic myelomonocytic leukemia (CMML) is a hematological neoplasm characterized by monocytosis, splenomegaly, thrombocytopenia, and anemia. Moreover, it is associated ... ...

Abstract	Chronic myelomonocytic leukemia (CMML) is a hematological neoplasm characterized by monocytosis, splenomegaly, thrombocytopenia, and anemia. Moreover, it is associated with
MeSH term(s)	Humans ; Aged, 80 and over ; Leukemia, Myelomonocytic, Chronic/complications ; Leukemia, Myelomonocytic, Chronic/genetics ; Germ-Line Mutation ; Genetic Predisposition to Disease ; Mutation ; Anemia ; Germ Cells ; Receptors, Colony-Stimulating Factor/genetics
Chemical Substances	CSF3R protein, human ; Receptors, Colony-Stimulating Factor
Language	English
Publishing date	2023-11-07
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242216021
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Article ; Online: Biofilm Inhibition of Inula viscosa (L.) Aiton and Globularia alypum L. Extracts Against Candida Infectious Pathogens and In Vivo Action on Galleria mellonella Model.

Asraoui, Fadoua / El Mansouri, Fouad / Cacciola, Francesco / Brigui, Jamal / Louajri, Adnane / Simonetti, Giovanna

Advanced biology

2023 Volume 7, Issue 12, Page(s) e2300081

Abstract: The increasing importance of fungal infections has fueled the search for new beneficial alternatives substance from plant extracts. The current study investigates the antifungal and antibiofilm activity of Inula viscosa (L.) Aiton and Globularia alypum ( ... ...

Abstract	The increasing importance of fungal infections has fueled the search for new beneficial alternatives substance from plant extracts. The current study investigates the antifungal and antibiofilm activity of Inula viscosa (L.) Aiton and Globularia alypum (L.) leaves extracts against Candida both in vitro and in vivo. The inhibition of planktonic and sessile Candida albicans and Candida glabrata growth using both leaf extracts are evaluated. Moreover; an in vivo infection model using Galleria mellonella larvae; infected and treated with the extracts are performed. All extracts show fungicidal activity; with a minimum fungicidal concentration (MFC) ranging from 128 to 512 µg mL
MeSH term(s)	Animals ; Inula ; Candidiasis/drug therapy ; Candida albicans ; Candida ; Antifungal Agents/pharmacology ; Antifungal Agents/therapeutic use ; Moths/microbiology ; Larva ; Biofilms
Chemical Substances	Antifungal Agents
Language	English
Publishing date	2023-08-23
Publishing country	Germany
Document type	Journal Article
ISSN	2701-0198
ISSN (online)	2701-0198
DOI	10.1002/adbi.202300081
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Article ; Online: Profile of free and conjugated quercetin content in different Italian wines

Simonetti, Giulia / Buiarelli, Francesca / Bernardini, Flaminia / Filippo, Patrizia Di / Riccardi, Carmela / Pomata, Donatella

Food Chemistry. 2022 July, v. 382 p.132377-

2022

Abstract: Quercetin and its structural derivatives are natural compounds belonging to the flavonoid class, widely distributed in plants. Beneficial physiological activities have been attributed to them, but some require deeper investigation. In this paper the ... ...

Abstract	Quercetin and its structural derivatives are natural compounds belonging to the flavonoid class, widely distributed in plants. Beneficial physiological activities have been attributed to them, but some require deeper investigation. In this paper the content of quercetin and five analogues (quercetin-3-glucoside, quercetin-3-rutinoside, quercetin-3-ramnoside, quercetin-3-arabinoglucoside, 4′-O-methylquercetin) were determined by HPLC-ESI-MS/MS in wines made of different varieties of red and white vines. The aim was a comparative study focusing on quercetin and on the contribution of related compounds in twenty wines coming from different part of Italy. Wines produced from Sangiovese and Nero d’Avola, monovarietal grapes, were richest in quercetin compounds and our results were compared to our previous study and to other investigations. The proposed method resulted simple, fast, economical, and suitable for the analysis of quercetin analogues without the need of hydrolysis and falls in the optic of a 360° characterization of active wine compounds, with nutraceutical properties.
Keywords	comparative study ; dietary supplements ; food chemistry ; hydrolysis ; quercetin ; wines ; Italy ; Quercetin glycosides ; Quercetin analogues ; Liquid chromatography tandem mass spectrometry
Language	English
Dates of publication	2022-07
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.132377
Database	NAL-Catalogue (AGRICOLA)

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