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  1. Article: Application of biomagnetic analysis technique using roadside trees for monitoring and identification of possible sources of atmospheric particulates in selected air pollution hotspots in Delhi, India

    Singh, Babita / Kaushik, Anubha

    Turkish National Committee for Air Pollution Research and Control Atmospheric pollution research journal. 2021 July, v. 12, no. 7

    2021  

    Abstract: With increasing atmospheric pollution and health issues associated with size of the particulate matter, it has become important to look for techniques that may improve the monitoring resolution. Magnetic bio-monitoring of particulate matter has been used ...

    Abstract With increasing atmospheric pollution and health issues associated with size of the particulate matter, it has become important to look for techniques that may improve the monitoring resolution. Magnetic bio-monitoring of particulate matter has been used in recent years in some countries as an approach for better spatial resolution that provides proxy indicators for the measurements over large areas. Delhi, which is one of the most polluted cities of not just India, but the whole world, is still probing to understand the possible sources. The present magnetic biomonitoring study was therefore, carried across different land use areas in some air pollution hotspots of Delhi, using common roadside tree species Morus alba, Ficus religiosa, Ficus virens and Ficus benghalensis to understand the magnitude and nature of the particulate pollution, and possible sources by studying magnetic properties (Magnetic susceptibility, Frequency-dependent susceptibility, S-ratio, and SIRM) of the dust deposited on leaves. Mass specific magnetic susceptibility (10⁻⁸ m³ kg⁻¹) values were found to follow the order: Traffic intersection area (25.6–66.5) > Industrial area (25.4–41.3) > Residential area (13.2–30.1) > Institutional area serving as control (2.7–6.6). High magnetic susceptibility values indicated particulates with ferrimagnetic grains of anthropogenic or technogenic origin. Frequency-dependent Susceptibility indicated dominance of coarse multidomain (MD) and Pseudo Single Domain (PSD) +MD grains in industrial area and major traffic intersection. Average S ratio across all study sites ranged from 0.92 to 0.99 indicating presence of soft magnetic mineral with low coercivity. High SIRM values (10⁻⁵Am² kg⁻¹) from 58.1 to 862.3 suggested prevalence of magnetite dominating atmospheric particulates particularly in traffic intersection and industrial area, and to some extent in residential area. Morus alba and Ficus religiosa were found more suitable bio-monitors and the technique provided useful information on size, mineralogy and possible source of the particulates.
    Keywords Ficus benghalensis ; Ficus religiosa ; Ficus virens ; Morus alba ; air pollution ; dust ; environmental monitoring ; indicator species ; land use ; magnetic susceptibility ; magnetism ; magnetite ; particulates ; research ; residential areas ; roadsides ; traffic ; trees ; India
    Language English
    Dates of publication 2021-07
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2645757-X
    ISSN 1309-1042
    ISSN 1309-1042
    DOI 10.1016/j.apr.2021.101113
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Mental health education in undergraduate nursing curricula across the universities in Nepal.

    Singh, Rakesh / Lamichhane, Deepika / Singh, Babita

    Asian journal of psychiatry

    2022  Volume 76, Page(s) 103225

    MeSH term(s) Curriculum ; Education, Nursing, Baccalaureate ; Health Education ; Humans ; Nepal ; Students, Nursing ; Universities
    Language English
    Publishing date 2022-07-19
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2456678-0
    ISSN 1876-2026 ; 1876-2018
    ISSN (online) 1876-2026
    ISSN 1876-2018
    DOI 10.1016/j.ajp.2022.103225
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Correction to: Role of Silent Information Regulator 1 (SIRT1) in Regulating Oxidative Stress and Inflammation.

    Ubaid, Saba / Rumman, Mohammad / Singh, Babita / Pandey, Shivani

    Inflammation

    2021  Volume 44, Issue 5, Page(s) 2142

    Language English
    Publishing date 2021-04-07
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 434408-x
    ISSN 1573-2576 ; 0360-3997
    ISSN (online) 1573-2576
    ISSN 0360-3997
    DOI 10.1007/s10753-021-01457-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Berberine ameliorates glucocorticoid-induced hyperglycemia: an in vitro and in vivo study.

    Gupta, Mrinal / Rumman, Mohammad / Singh, Babita / Mahdi, Abbas Ali / Pandey, Shivani

    Naunyn-Schmiedeberg's archives of pharmacology

    2023  Volume 397, Issue 3, Page(s) 1647–1658

    Abstract: Berberine (BBR), a bioactive compound isolated from Coptidis Rhizoma, possesses diverse pharmacological activities including anti-bacterial, anti-inflammatory, antitumor, hypolipidemic, and anti-diabetic. However, its role as an anti-diabetic agent in ... ...

    Abstract Berberine (BBR), a bioactive compound isolated from Coptidis Rhizoma, possesses diverse pharmacological activities including anti-bacterial, anti-inflammatory, antitumor, hypolipidemic, and anti-diabetic. However, its role as an anti-diabetic agent in animal models of dexamethasone (Dex)-induced diabetes remains unknown. Studies have shown that natural compounds including aloe, caper, cinnamon, cocoa, green and black tea, and turmeric can be used for treating Type 2 diabetes mellitus (DM). Compared to conventional drugs, natural compounds have less side effects and are easily available. Herein, we studied the anti-diabetic effects of BBR in a mice model of Dex-induced diabetes. HepG2 cell line was used for glucose release and glycogen synthesis studies. Cell proliferation was measured by methylthiotetrazole (MTT) assay. For animal studies, mice were treated with Dex (2 mg/kg, i.m.) for 30 days and effect of BBR at the doses 100, 200, and 500 mg/kg (p.o.) was analyzed. Glucose, insulin, and pyruvate tests were performed for evaluating the development of the diabetic model. Echo MRI was performed to assess the fat mass. Further, to elucidate the mechanism of action of BBR, mRNA expression of genes regulating gluconeogenesis, glucose uptake, and glycolysis was analyzed. In vitro BBR had no impact on cell viability up to a concentration of 50 μM. Moreover, BBR suppressed the hepatic glucose release and improved glucose tolerance in HepG2 cells. In vivo, BBR improved glucose homeostasis in diabetic mice as evidenced by enhanced glucose clearance, increased glycolysis, elevated glucose uptake, and decreased gluconeogenesis. Further, Dex treatment increased the total fat mass in mice, which was ameliorated by BBR treatment. BBR improves glucose tolerance by increasing glucose clearance, inhibiting hepatic glucose release, and decreasing obesity. Thus, BBR may become a potential therapeutic agent for treating glucocorticoid-induced diabetes and obesity in the future.
    MeSH term(s) Mice ; Animals ; Berberine/pharmacology ; Berberine/therapeutic use ; Diabetes Mellitus, Type 2/drug therapy ; Glucocorticoids/pharmacology ; Glucocorticoids/therapeutic use ; Diabetes Mellitus, Experimental/chemically induced ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Hyperglycemia/chemically induced ; Hyperglycemia/drug therapy ; Glucose/metabolism ; Anti-Inflammatory Agents/therapeutic use ; Obesity/drug therapy
    Chemical Substances Berberine (0I8Y3P32UF) ; Glucocorticoids ; Glucose (IY9XDZ35W2) ; Anti-Inflammatory Agents
    Language English
    Publishing date 2023-09-14
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 121471-8
    ISSN 1432-1912 ; 0028-1298
    ISSN (online) 1432-1912
    ISSN 0028-1298
    DOI 10.1007/s00210-023-02703-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Association of salivary cortisol and psychological burden in patients with oral cancer and oral potentially malignant disorders: A prospective study.

    Kumar, Sumit / Kumar, Kapila / Singh, Babita / Mehrotra, Divya / Mahdi, Abbas A

    Journal of cancer research and therapeutics

    2023  Volume 19, Issue Supplement, Page(s) S0

    Abstract: Introduction: This study aimed to find an association between psychological burden (in terms of depression, anxiety, and stress) and salivary cortisol among oral cancer (OC) and oral potentially malignant disorder (OPMD) patients at various time frames.! ...

    Abstract Introduction: This study aimed to find an association between psychological burden (in terms of depression, anxiety, and stress) and salivary cortisol among oral cancer (OC) and oral potentially malignant disorder (OPMD) patients at various time frames.
    Methods: In total, 50 patients with OC and OPMD were studied after their informed consent along with 30 healthy controls. Depression, anxiety, and stress scale-21 (DASS-21) was administered and saliva was collected (non-invasively) at different stages including the time of diagnosis, one and three months after intervention (medical or surgical). To avoid diurnal variation, saliva was collected twice (morning and evening). To assess the linear relationship between depression, anxiety, and stress with salivary cortisol, a partial correlation was calculated.
    Results: Comparison of salivary cortisol levels among control, OC, and OPMD groups showed a statistically significant difference in both morning and evening values at different point of time intervals. Higher values of salivary cortisol were observed in OC patients (both morning and evening) in comparison to the OPMD or control group. A positive correlation was discerned between stress and salivary cortisol in both OPMD and OC patients, while no association was found for depression and anxiety domains.
    Conclusion: The measurement of salivary cortisol effectively demonstrates raised stress levels in OPMD as well as OC patients. Therefore, it is recommended to institute stress management interventions in the patients as part of the treatment of OPMD and OC.
    MeSH term(s) Humans ; Hydrocortisone ; Prospective Studies ; Mouth Neoplasms/therapy ; Precancerous Conditions ; Anxiety/etiology ; Syndrome ; Saliva
    Chemical Substances Hydrocortisone (WI4X0X7BPJ)
    Language English
    Publishing date 2023-05-08
    Publishing country India
    Document type Journal Article
    ZDB-ID 2187633-2
    ISSN 1998-4138 ; 0973-1482
    ISSN (online) 1998-4138
    ISSN 0973-1482
    DOI 10.4103/jcrt.jcrt_530_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: SIRT1 Mediates Neuroprotective and Neurorescue Effects of Camel α-Lactalbumin and Oleic Acid Complex on Rotenone-Induced Parkinson's Disease.

    Ubaid, Saba / Pandey, Shivani / Akhtar, Mohd Sohail / Rumman, Mohammad / Singh, Babita / Mahdi, Abbas Ali

    ACS chemical neuroscience

    2022  Volume 13, Issue 8, Page(s) 1263–1272

    Abstract: Parkinson's disease (PD) is the second most common devastating neurodegenerative disorder. Presently used therapies for PD have severe side effects and are limited to only temporary improvement. Therefore, a new therapeutic approach to treat PD urgently ... ...

    Abstract Parkinson's disease (PD) is the second most common devastating neurodegenerative disorder. Presently used therapies for PD have severe side effects and are limited to only temporary improvement. Therefore, a new therapeutic approach to treat PD urgently needs to be developed. α-Lactalbumin, the most abundant milk protein in camel milk, has been attributed to various medicinal properties. This study intended to investigate the neuroprotective efficacy of the camel α-lactalbumin and oleic acid (CLOA) complex. One mechanism postulated to underlie neuroprotection by the CLOA complex is the induction of silent information regulatory protein (SIRT1). SIRT1 is known to be involved in several pathological and physiological processes, and it has been suggested that SIRT1 plays a protective role in PD. Oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis are involved in PD pathogenesis. Our results revealed that SIRT1 inhibits oxidative stress by maintaining HIF-1α in a deacetylated state. SIRT1 upregulates the expression of FOXO3a and HSF-1, thus inhibiting apoptosis and maintaining the homeostasis of cellular proteins. Increased SIRT1 expression reduces the levels of TNF-α, IL-6, and IL-8, which in turn inhibits neuroinflammation. In addition to SIRT1, the CLOA complex also enhances the expression of survivin and leptin and promotes the survival of neuroblastoma cells. Altogether, our results suggest that the CLOA complex might be a novel therapeutic molecule that could ameliorate neuronal cell damage in PD.
    MeSH term(s) Animals ; Camelus/metabolism ; Lactalbumin/metabolism ; Lactalbumin/pharmacology ; Lactalbumin/therapeutic use ; Neuroprotection ; Neuroprotective Agents/pharmacology ; Neuroprotective Agents/therapeutic use ; Oleic Acid/pharmacology ; Oleic Acid/therapeutic use ; Oxidative Stress ; Parkinson Disease/drug therapy ; Rotenone ; Sirtuin 1/metabolism ; Sirtuin 1/pharmacology ; Sirtuin 1/therapeutic use
    Chemical Substances Neuroprotective Agents ; Rotenone (03L9OT429T) ; Oleic Acid (2UMI9U37CP) ; Lactalbumin (9013-90-5) ; Sirtuin 1 (EC 3.5.1.-)
    Language English
    Publishing date 2022-04-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/acschemneuro.1c00876
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Genistein suppresses microglial activation and inhibits apoptosis in different brain regions of hypoxia-exposed mice model of amnesia.

    Rumman, Mohammad / Pandey, Shivani / Singh, Babita / Gupta, Mrinal / Mahdi, Abbas Ali

    Metabolic brain disease

    2022  Volume 37, Issue 7, Page(s) 2521–2532

    Abstract: Genistein (GE) or 4',5,7-trihydroxyflavone, a plant derived isoflavone, is a biologically active compound having several beneficial properties. Studies showed that GE possesses anti-neoplastic, anti-tumor, anti-helminthic, anti-oxidant, and anti- ... ...

    Abstract Genistein (GE) or 4',5,7-trihydroxyflavone, a plant derived isoflavone, is a biologically active compound having several beneficial properties. Studies showed that GE possesses anti-neoplastic, anti-tumor, anti-helminthic, anti-oxidant, and anti-inflammatory activities. Herein, we investigated the neuroprotective effects of GE in a mouse model of hypoxia-induced amnesia. Mice were exposed to hypoxic conditions (10% O2) in a designated hypoxia chamber and co-treated with GE (10, 20, or 30 mg/kg) for 4 weeks. Following this, behavioral tests were performed to evaluate memory performance. We assessed microglial activation in the hippocampus, amygdala, and pre-frontal cortex (PFC) regions by evaluating the Iba-1 and GFAP transcript levels, and MIP-1β, Cox-2, and IL6 protein levels. Apoptosis was assessed by evaluating Bax, BAD, and Bcl-2 mRNA levels, and caspase-3 activity. To uncover the underlying molecular mechanism, we evaluated the levels of Nrf2, HO-1, and NQO1 in different brain regions of mice from all groups. Results showed that hypoxia-exposed mice have reduced performance in the behavioral tests and GE treatment enhanced the memory performance in hypoxia-exposed mice. Moreover, hypoxia-exposed mice showed increased expression of microglial activation markers and enhanced apoptosis in the hippocampus, amygdala, and PFC. GE treatment suppressed microglial activation and prevented apoptosis in the brain of hypoxia-exposed mice. Furthermore, hypoxia-exposure reduced the expression of Nrf2, NQO1, and HO-1 while GE treatment ameliorated this decrease in different regions of hypoxia-exposed mice brain. In conclusion, GE prevents cognitive dysfunction by suppressing microglial activation and inhibiting apoptosis in the hypoxia-exposed mice brain.
    MeSH term(s) Animals ; Mice ; Genistein/pharmacology ; NF-E2-Related Factor 2/metabolism ; Neuroprotective Agents/pharmacology ; Neuroprotective Agents/therapeutic use ; Interleukin-6/metabolism ; Antioxidants/pharmacology ; Caspase 3/metabolism ; Microglia/metabolism ; Cyclooxygenase 2/metabolism ; Chemokine CCL4/metabolism ; bcl-2-Associated X Protein/metabolism ; Amnesia/chemically induced ; Apoptosis ; Brain/metabolism ; Hypoxia/complications ; Hypoxia/drug therapy ; Disease Models, Animal ; Anti-Inflammatory Agents/pharmacology ; RNA, Messenger
    Chemical Substances Genistein (DH2M523P0H) ; NF-E2-Related Factor 2 ; Neuroprotective Agents ; Interleukin-6 ; Antioxidants ; Caspase 3 (EC 3.4.22.-) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Chemokine CCL4 ; bcl-2-Associated X Protein ; Anti-Inflammatory Agents ; RNA, Messenger
    Language English
    Publishing date 2022-07-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632824-6
    ISSN 1573-7365 ; 0885-7490
    ISSN (online) 1573-7365
    ISSN 0885-7490
    DOI 10.1007/s11011-022-01039-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet.

    Kumar, Verandra / Singh, Babita / Kumar Singh, Roshan / Sharma, Namisha / Muthamilarasan, Mehanathan / Sawant, Samir V / Prasad, Manoj

    Journal of experimental botany

    2023  Volume 75, Issue 3, Page(s) 1098–1111

    Abstract: Climate change inflicts several stresses on plants, of which dehydration stress severely affects growth and productivity. C4 plants possess better adaptability to dehydration stress; however, the role of epigenetic modifications underlying this trait is ... ...

    Abstract Climate change inflicts several stresses on plants, of which dehydration stress severely affects growth and productivity. C4 plants possess better adaptability to dehydration stress; however, the role of epigenetic modifications underlying this trait is unclear. In particular, the molecular links between histone modifiers and their regulation remain elusive. In this study, genome-wide H3K9 acetylation (H3K9ac) enrichment using ChIP-sequencing was performed in two foxtail millet cultivars with contrasting dehydration tolerances (IC403579, cv. IC4-tolerant, and IC480117, cv. IC41-sensitive). It revealed that a histone deacetylase, SiHDA9, was significantly up-regulated in the sensitive cultivar. Further characterization indicated that SiHDA9 interacts with SiHAT3.1 and SiHDA19 to form a repressor complex. SiHDA9 might be recruited through the SiHAT3.1 recognition sequence onto the upstream of dehydration-responsive genes to decrease H3K9 acetylation levels. The silencing of SiHDA9 resulted in the up-regulation of crucial genes, namely, SiRAB18, SiRAP2.4, SiP5CS2, SiRD22, SiPIP1;4, and SiLHCB2.3, which imparted dehydration tolerance in the sensitive cultivar (IC41). Overall, the study provides mechanistic insights into SiHDA9-mediated regulation of dehydration stress response in foxtail millet.
    MeSH term(s) Dehydration ; Setaria Plant/genetics ; Up-Regulation ; Phenotype ; Histone Deacetylases/genetics ; Gene Expression Regulation, Plant ; Stress, Physiological/genetics ; Plant Proteins/genetics
    Chemical Substances Histone Deacetylases (EC 3.5.1.98) ; Plant Proteins
    Language English
    Publishing date 2023-10-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    DOI 10.1093/jxb/erad425
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The role of alternative splicing in cancer.

    Singh, Babita / Eyras, Eduardo

    Transcription

    2017  Volume 8, Issue 2, Page(s) 91–98

    Abstract: The functional capacity of cells is defined by the transcriptome. Many recent studies have identified variations in the transcriptome of tumors due to alternative splicing changes, as well as mutations in splicing factors and regulatory signals in most ... ...

    Abstract The functional capacity of cells is defined by the transcriptome. Many recent studies have identified variations in the transcriptome of tumors due to alternative splicing changes, as well as mutations in splicing factors and regulatory signals in most tumor types. Some of these alterations have been linked to tumor progression, metastasis, therapy resistance, and other oncogenic processes. Here, we describe the different mechanisms that drive splicing changes in tumors and their impact in cancer. Motivated by the current evidence, we propose a model whereby a subset of the splicing patterns contributes to the definition of specific tumor phenotypes, and may hold potential for the development of novel clinical biomarkers and therapeutic approaches.
    Language English
    Publishing date 2017-03-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2646974-1
    ISSN 2154-1272 ; 2154-1264
    ISSN (online) 2154-1272
    ISSN 2154-1264
    DOI 10.1080/21541264.2016.1268245
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Repurposing of FDA approved kinase inhibitor bosutinib for mitigation of radiation induced damage via inhibition of JNK pathway.

    Singh, Babita / Patwardhan, Raghavendra S / Pal, Debojyoti / Maurya, Dharmendra K / Singh, Beena G / Checker, Rahul / Sharma, Deepak / Sandur, Santosh K

    Toxicology and applied pharmacology

    2023  Volume 482, Page(s) 116792

    Abstract: Radiotherapy is a common modality for cancer treatment. However, it is often associated with normal tissue toxicity in 20-80% of the patients. Radioprotectors can improve the outcome of radiotherapy by selectively protecting normal cells against ... ...

    Abstract Radiotherapy is a common modality for cancer treatment. However, it is often associated with normal tissue toxicity in 20-80% of the patients. Radioprotectors can improve the outcome of radiotherapy by selectively protecting normal cells against radiation toxicity. In the present study, compound libraries containing 54 kinase inhibitors and 80 FDA-approved drugs were screened for radioprotection of lymphocytes using high throughput cell analysis. A second-generation FDA-approved kinase inhibitor, bosutinib, was identified as a potential radioprotector for normal cells. The radioprotective efficacy of bosutinib was evinced from a reduction in radiation induced DNA damage, caspase-3 activation, DNA fragmentation and apoptosis. Oral administration of bosutinib protected mice against whole body irradiation (WBI) induced morbidity and mortality. Bosutinib also reduced radiation induced bone-marrow aplasia and hematopoietic damage in mice exposed to 4 Gy and 6 Gy dose of WBI. Mechanistic studies revealed that the radioprotective action of bosutinib involved interaction with cellular thiols and modulation of JNK pathway. The addition of glutathione and N-acetyl cysteine significantly reduced the radioprotective efficacy of bosutinib. Moreover, bosutinib did not protect cancer cells against radiation induced toxicity. On the contrary, bosutinib per se exhibited anticancer activity against human cancer cell lines. The results highlight possible use of bosutinib as a repurposable radioprotective agent for mitigation of radiation toxicity in cancer patients undergoing radiotherapy.
    MeSH term(s) Animals ; Humans ; Mice ; Aniline Compounds/pharmacology ; Aniline Compounds/therapeutic use ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; DNA Damage ; Drug Repositioning ; MAP Kinase Signaling System ; Nitriles/pharmacology ; Nitriles/therapeutic use ; Quinolines/pharmacology ; Quinolines/therapeutic use ; Radiation Injuries/prevention & control ; Radiation-Protective Agents/pharmacology ; Radiation-Protective Agents/therapeutic use
    Chemical Substances Aniline Compounds ; Antineoplastic Agents ; bosutinib (5018V4AEZ0) ; Nitriles ; Quinolines ; Radiation-Protective Agents
    Language English
    Publishing date 2023-12-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 204477-8
    ISSN 1096-0333 ; 0041-008X
    ISSN (online) 1096-0333
    ISSN 0041-008X
    DOI 10.1016/j.taap.2023.116792
    Database MEDical Literature Analysis and Retrieval System OnLINE

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