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Article ; Online: Photoperiodic regulation of the splenocyte immune responses in the fresh water snake, Natrixpiscator.

Tripathi, Manish Kumar / Singh, Ramesh

Developmental and comparative immunology

2022 Volume 132, Page(s) 104403

Abstract: Photoperiod and melatonin are important regulators of immunity. We hypothesized that these two factors play an important role in the regulation of immune responses in the Natrix piscator. Animals were kept in either short or long days and splenocyte ... ...

Abstract	Photoperiod and melatonin are important regulators of immunity. We hypothesized that these two factors play an important role in the regulation of immune responses in the Natrix piscator. Animals were kept in either short or long days and splenocyte immune responses were studied. Respiratory burst activity of splenocytes was assessed through reduction of nitrobluetetrazolium salt while production of nitric oxide was assessed indirectly by nitrite assay. Density gradient centrifugation was used to isolate splenic lymphocytes which were utilized to study proliferation with and without mitogens. Super oxide production by splenocytes was reduced significantly in the cultures obtained from animals kept either in short or long days. Nitrite release was decreased when animals were subjected to long days. The photoperiodic alterations acted differentially on proliferations of the splenic lymphocytes. Spontaneous and mitogen-induced proliferation of splenic lymphocytes were enhanced in cultures obtained from snakes maintained in short days when compared with cultures from snakes obtained either from long day or natural day length conditions. In vitro melatonin significantly enhanced the splenic lymphocyte proliferation of the cultures obtained from animals kept in long days when compared with splenic lymphocyte proliferations of the cultures obtained from long day animals or the animals kept in natural day length conditions. We found evidence which suggest that photoperiod may influence seasonal energy budgets and induce adjustments which optimize energy allocation for costly physiological processes such as immune function. In seasonally breeding animals such as Natrix piscator, the pineal hormone melatonin assists in the suppression of reproduction and elevation of immunity, which are the crucial adaptation for perpetuation of species.
MeSH term(s)	Animals ; Colubridae ; Fresh Water ; Lymphocyte Activation ; Melatonin ; Nitrites ; Photoperiod ; Seasons ; Spleen
Chemical Substances	Nitrites ; Melatonin (JL5DK93RCL)
Language	English
Publishing date	2022-03-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2022.104403
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Article: Effect of different soil amendments and sowing date on wilt disease caused by Fusarium oxysporum f. sp. lentis of lentil

Ram, Kamlesh / Singh, Ramesh

Annals of plant protection sciences. 2022 Mar., v. 30, no. 1

2022

Abstract: Lentil is one of the major Rabi pulse crop grown in India. Seven soil amendments such as Pyrite and Gypsum @ 2.0 gm/kg soil and Neem cake, Mustard cake, Paddy straw and Wheat straw @ 20 gm/kg soil were incorporated in the soil eight days before sowing of ...

Abstract	Lentil is one of the major Rabi pulse crop grown in India. Seven soil amendments such as Pyrite and Gypsum @ 2.0 gm/kg soil and Neem cake, Mustard cake, Paddy straw and Wheat straw @ 20 gm/kg soil were incorporated in the soil eight days before sowing of healthy lentil seed of “Rath local” variety. Among the Neem cake @ 20 gm/kg soil was found significantly superior over all the treatments resulting minimum wilt disease incidence (34.80 and 35.90%) maximum disease reduction over control (57.24 and 56.25%) maximum yield (6.30 and 6.50 q/ha.) and maximum yield increase over control (186.36 and 242.10%) in the years 2018–19 and 2019–20 respectively. Wheat straw was the least effective showing maximum disease incidence of (61 and 61.53%) minimum disease reduction over control of (25.06 and 25%), minimum yield of (3.10 and 3.05 q/ha.) and minimum yield increased over control of 40.90 and 60.52% in the years 2018–19 and 2019–20 respectively. The date of sowing of the lentil seed was significantly affect the disease incidence and yield of the crops. The minimum wilt disease incidence of 10.26 and 15.30% with maximum yield of 10.95 and 10.06 q/ha was recorded when the seed was sown on 5ᵗʰ November, date of sowing in the years of 2019–20 and 2018–19, respectively, followed by 29ᵗʰ October, 22ⁿᵈ October, 15ᵗʰ October and 8ᵗʰ October. The maximum disease incidence (32.73 and 30%) with minimum yield (2.50 and 3.0 q/ha) was observed on the 1ˢᵗ October date of sowing in the years 2018–19 and 2019–20 respectively. The average wilt disease incidence was decreased with increasing crop yield at 1ˢᵗ October to 5ᵗʰ November date of sowing significantly.
Keywords	Fusarium oxysporum ; crop yield ; disease incidence ; gypsum ; lentils ; mustard oil cake ; neem cake ; plant protection ; pyrite ; rice straw ; soil ; vascular wilt ; wheat straw ; India
Language	English
Dates of publication	2022-03
Size	p. 37-41.
Publishing place	Society Of Plant Protection Sciences
Document type	Article
ISSN	0974-0163
DOI	10.5958/0974-0163.2022.00008.8
Database	NAL-Catalogue (AGRICOLA)

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Article: Incidence of downey mildew of bitter gourd in eastern Uttar Pradesh

Prajapati, Anand Kumar / Singh, Ramesh

Annals of plant protection sciences. 2022 Mar., v. 30, no. 1

2022

Abstract: Survey was conducted for bitter gourd disease incidence in the five districts of eastern Uttar Pradesh in 2018–19 and 2019–20. The five districts with two tahsil was selected from Jaunpur, Varanasi, Azamgarh, Sultanpur and Mirzapur. Two village were ... ...

Abstract	Survey was conducted for bitter gourd disease incidence in the five districts of eastern Uttar Pradesh in 2018–19 and 2019–20. The five districts with two tahsil was selected from Jaunpur, Varanasi, Azamgarh, Sultanpur and Mirzapur. Two village were selected in each tahsil with former field and the data were recorded separately. Jaunpur was severely infected with the maximum average PDI (25.58%) followed by Mirzapur (24.16%), Sultanpur (22.49%)and the minimum average PDI (15.41%) was recorded in Azamgarh in the year 2018–19. Similarly, in the year 2019–20 Mirzapur was found to be most affected district with an average PDI of 24.74% followed by Jaunpur (23.41%), Varanasi (22.61%), Sultanpur (21.49%) and the minimum average PDI (20.91%) was recorded in Azamgarh in year 2019–20. Among the several districts the maximum average PDI in the year 2018–19 was found in Jaunpur (25.58%) and minimum average PDI was recorded in Azamgarh (15.41%). In year 2019–20 the maximum average PDI was found in Mirzapur (27.74%) and minimum average PDI (20.91%) was recorder in Azamgarh District.
Keywords	Momordica charantia ; disease incidence ; mildews ; plant protection ; surveys ; villages ; India
Language	English
Dates of publication	2022-03
Size	p. 42-44.
Publishing place	Society Of Plant Protection Sciences
Document type	Article
ISSN	0974-0163
DOI	10.5958/0974-0163.2022.00009.X
Database	NAL-Catalogue (AGRICOLA)

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Article: Photoperiodic regulation of the splenocyte immune responses in the fresh water snake, Natrixpiscator

Tripathi, Manish Kumar / Singh, Ramesh

Developmental and comparative immunology. 2022 July, v. 132

2022

Abstract	Photoperiod and melatonin are important regulators of immunity. We hypothesized that these two factors play an important role in the regulation of immune responses in the Natrix piscator. Animals were kept in either short or long days and splenocyte immune responses were studied. Respiratory burst activity of splenocytes was assessed through reduction of nitrobluetetrazolium salt while production of nitric oxide was assessed indirectly by nitrite assay. Density gradient centrifugation was used to isolate splenic lymphocytes which were utilized to study proliferation with and without mitogens. Super oxide production by splenocytes was reduced significantly in the cultures obtained from animals kept either in short or long days. Nitrite release was decreased when animals were subjected to long days. The photoperiodic alterations acted differentially on proliferations of the splenic lymphocytes. Spontaneous and mitogen-induced proliferation of splenic lymphocytes were enhanced in cultures obtained from snakes maintained in short days when compared with cultures from snakes obtained either from long day or natural day length conditions. In vitro melatonin significantly enhanced the splenic lymphocyte proliferation of the cultures obtained from animals kept in long days when compared with splenic lymphocyte proliferations of the cultures obtained from long day animals or the animals kept in natural day length conditions. We found evidence which suggest that photoperiod may influence seasonal energy budgets and induce adjustments which optimize energy allocation for costly physiological processes such as immune function. In seasonally breeding animals such as Natrix piscator, the pineal hormone melatonin assists in the suppression of reproduction and elevation of immunity, which are the crucial adaptation for perpetuation of species.
Keywords	Natrix ; density gradient centrifugation ; energy ; freshwater ; immune response ; lymphocyte proliferation ; melatonin ; mitogens ; nitric oxide ; nitrites ; photoperiod ; respiratory burst ; snakes ; splenocytes
Language	English
Dates of publication	2022-07
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2022.104403
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives.

Kosara, Sanjay / Singh, Ramesh / Bhatia, Dhiraj

Nanoscale advances

2023 Volume 6, Issue 2, Page(s) 386–401

Abstract: DNA nanotechnology has significantly progressed in the last four decades, creating nucleic acid structures widely used in various biological applications. The structural flexibility, programmability, and multiform customization of DNA-based ... ...

Abstract	DNA nanotechnology has significantly progressed in the last four decades, creating nucleic acid structures widely used in various biological applications. The structural flexibility, programmability, and multiform customization of DNA-based nanostructures make them ideal for creating structures of all sizes and shapes and multivalent drug delivery systems. Since then, DNA nanotechnology has advanced significantly, and numerous DNA nanostructures have been used in biology and other scientific disciplines. Despite the progress made in DNA nanotechnology, challenges still need to be addressed before DNA nanostructures can be widely used in biological interfaces. We can open the door for upcoming uses of DNA nanoparticles by tackling these issues and looking into new avenues. The historical development of various DNA nanomaterials has been thoroughly examined in this review, along with the underlying theoretical underpinnings, a summary of their applications in various fields, and an examination of the current roadblocks and potential future directions.
Language	English
Publishing date	2023-12-19
Publishing country	England
Document type	Journal Article ; Review
ISSN	2516-0230
ISSN (online)	2516-0230
DOI	10.1039/d3na00692a
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Article ; Online: Spatio-temporal evolution of groundwater quality and its health risk assessment in Punjab (India) during 2000-2020.

Romana, Harsimranjit Kaur / Singh, Ramesh P / Shukla, Dericks Praise

Environmental science and pollution research international

2023

Abstract: The state known as the bread basket of India has now been defamed as the cancer capital of the country. The toxicity of groundwater associated with the declining water level is reported in recent years. However, an extensive temporal and spatial analysis ...

Abstract	The state known as the bread basket of India has now been defamed as the cancer capital of the country. The toxicity of groundwater associated with the declining water level is reported in recent years. However, an extensive temporal and spatial analysis is required to identify hotspots. In this study, spatial tools are utilized to understand the evolution of groundwater in Punjab (> 315 sites) for the last two decades (2000-2020) for drinking purposes using the water quality index (WQI). The data for pH, electric conductivity (EC), bicarbonate (HCO
Language	English
Publishing date	2023-08-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-29200-6
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Article ; Online: One Limb or Two? Does It Make a Difference in Femoral Venoarterial Extracorporeal Membrane Oxygenation?

Dalton, Heidi J / Singh, Ramesh / McMullan, D Michael

Critical care medicine

2023 Volume 52, Issue 1, Page(s) 153–155

MeSH term(s)	Extracorporeal Membrane Oxygenation ; Risk Factors ; Retrospective Studies ; Extremities ; Femoral Artery
Language	English
Publishing date	2023-12-14
Publishing country	United States
Document type	Editorial ; Comment
ZDB-ID	197890-1
ISSN	1530-0293 ; 0090-3493
ISSN (online)	1530-0293
ISSN	0090-3493
DOI	10.1097/CCM.0000000000006107
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Zs.A 1261: Show issues

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Article ; Online: Declining groundwater and its impacts along Ganga riverfronts using combined Sentinel-1, GRACE, water levels, and rainfall data.

Raju, Ashwani / Singh, Ramesh P / Kannojiya, Praveen Kumar / Patel, Abhinav / Singh, Saurabh / Sinha, Mitali

The Science of the total environment

2024 Volume 920, Page(s) 170932

Abstract: The Indo-Gangetic Plains (IGP) in northern India are vast alluvial tracts with huge shallow aquifers, densely populated and agriculturally productive regions. In the last few decades, IGP has been facing water scarcity driven by erratic monsoon dynamics, ...

Abstract	The Indo-Gangetic Plains (IGP) in northern India are vast alluvial tracts with huge shallow aquifers, densely populated and agriculturally productive regions. In the last few decades, IGP has been facing water scarcity driven by erratic monsoon dynamics, anthropogenic activity, and hydroclimatic variability. In urban centers, continuous groundwater withdrawal leads to high stress, affecting surface deformation and a threat to buildings and infrastructures. An attempt has been made to explore the possible linkage and coupling between groundwater level, hydroclimatic variables, and subsidence in the Central Ganga Plains (CGP), in Varanasi metropolis using the combined multisensory multitemporal data, Sentinel-1 (2017-2023), GRACE (2003-2023), groundwater levels (1998-2023), and precipitation (2002-2023). Long-term hydrological response in the CGP shows continuous depletion (14.6 ± 5.6 mm/yr) in response to precipitation variability. Results show spatiotemporal variations between GWS, and precipitation estimate with nonlinear trend response due to associated inter-annual/inter-seasonal climate variability and anthropogenic water withdrawal, specifically during the observed drought years. The significant storage response in the urban center compared to a regional extent suggests the potential impact of exponentially increasing urbanization and building hydrological stress in the cities. The implications of reducing storage capacity show measured land subsidence (∼2-8 mm/yr) patterns developed along the meandering stretch of the Ganga riverfronts in Varanasi. The groundwater level data from the piezometric supports the hydroclimatic variables and subsidence coupling. Considering the vital link between water storage, food security, and socioeconomic growth, the results of this study require systematic inclusion in water management strategies as climate change seriously impacts water resources in the future.
Language	English
Publishing date	2024-02-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.170932
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Article ; Online: Cationic lipid modification of DNA tetrahedral nanocages enhances their cellular uptake.

Singh, Ramesh / Yadav, Pankaj / Naveena A, Hema / Bhatia, Dhiraj

Nanoscale

2023 Volume 15, Issue 3, Page(s) 1099–1108

Abstract: Self-assembled DNA nanocages are among the most promising candidates for bioimaging and payload delivery into cells. DNA nanocages have great potential to efficiently address drug resistance and nucleic acid delivery problems due to precise control of ... ...

Abstract	Self-assembled DNA nanocages are among the most promising candidates for bioimaging and payload delivery into cells. DNA nanocages have great potential to efficiently address drug resistance and nucleic acid delivery problems due to precise control of their shape and size, and excellent biocompatibility. Although DNA nanostructures demonstrate some cellular uptake, because they bear a highly negative charge, the uptake of tetrahedral nanostructures is hindered by electrostatic repulsion. In this study, we describe a method to enhance the cellular uptake of DNA nanostructures using a binary system containing DNA and a positively charged head group with a hydrophobic lipid chain containing lipids for cellular internalization. Here we represent the functionalization of a model cage, DNA tetrahedron (TD) with a cationic lipid,
MeSH term(s)	DNA/chemistry ; Quaternary Ammonium Compounds ; Drug Delivery Systems ; Lipids ; Transfection ; Liposomes
Chemical Substances	N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium (104162-48-3) ; DNA (9007-49-2) ; Quaternary Ammonium Compounds ; Lipids ; Liposomes
Language	English
Publishing date	2023-01-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d2nr05749b
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Article ; Online: Self-Assembled DNA Nanocages Promote Cell Migration and Differentiation of Human Umbilical Vein Endothelial Cells.

Gada, Anjali Rajwar / Vaswani, Payal / Singh, Ramesh / Bhatia, Dhiraj

Chembiochem : a European journal of chemical biology

2023 Volume 24, Issue 7, Page(s) e202200634

Abstract: DNA nanocages have been explored for abilities to influence cellular behavior and functions. Recent times have seen the development of new emergent functionalities of DNA nanodevices as a class of biomaterials with an immense capacity to interface with ... ...

Abstract	DNA nanocages have been explored for abilities to influence cellular behavior and functions. Recent times have seen the development of new emergent functionalities of DNA nanodevices as a class of biomaterials with an immense capacity to interface with biological systems and with vast potential in disease diagnosis and therapeutics. Being chemically robust and biocompatible in nature, DNA nanocages have been surface modified and structurally fine-tuned to find emerging applications in the field of stem-cell therapy and tissue regeneration. DNA nanocages can be used for therapeutic angiogenesis that involves the induction of blood vessel formation and can be used to treat ischemic diseases like stroke or heart failure. This work addresses the effect of DNA nanocages' structural topology on their capacity to stimulate endothelial cell angiogenesis. We tested a panel of four DNA nanocage geometries and checked their potential on the differentiation of human umbilical vein endothelial cells (HUVECs). While different DNA nanocage geometries showed successful induction of angiogenesis and cell migration in HUVECs, tetrahedral DNA cages showed the maximum uptake and angiogenesis potential, thus indicating that not only the composition of materials, but also the 3D arrangement of ligands might play role in stimulating angiogenesis.
MeSH term(s)	Humans ; Human Umbilical Vein Endothelial Cells ; Neovascularization, Physiologic/genetics ; Cell Movement ; Cell Differentiation ; DNA/metabolism
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2023-02-28
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020469-3
ISSN	1439-7633 ; 1439-4227
ISSN (online)	1439-7633
ISSN	1439-4227
DOI	10.1002/cbic.202200634
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