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  1. Article: Targeting envelope proteins of poxviruses to repurpose phytochemicals against monkeypox: An

    Gulati, Pallavi / Chadha, Jatin / Harjai, Kusum / Singh, Sandeepa

    Frontiers in microbiology

    2023  Volume 13, Page(s) 1073419

    Abstract: The monkeypox virus (MPXV) has become a major threat due to the increasing global caseload and the ongoing multi-country outbreak in non-endemic territories. Due to limited research in this avenue and the lack of intervention strategies, the present ... ...

    Abstract The monkeypox virus (MPXV) has become a major threat due to the increasing global caseload and the ongoing multi-country outbreak in non-endemic territories. Due to limited research in this avenue and the lack of intervention strategies, the present study was aimed to virtually screen bioactive phytochemicals against envelope proteins of MPXV
    Language English
    Publishing date 2023-01-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1073419
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Highly Efficient and Rapid Plant Regeneration in Citrus sinensis

    Singh, Sandeepa / Rajam, Manchikatla Venkat

    Journal of plant biochemistry and biotechnology. 2010 July, v. 19, no. 2

    2010  

    Abstract: Sweet orange (Citrus sinensis L Osbeck, var Nagpur) was explored for efficient multiple shoot regeneration and rooting in different media. The influence of phytohormones and carbon source on the in vitro morphogenesis of sweet orange epicotyl explants ... ...

    Abstract Sweet orange (Citrus sinensis L Osbeck, var Nagpur) was explored for efficient multiple shoot regeneration and rooting in different media. The influence of phytohormones and carbon source on the in vitro morphogenesis of sweet orange epicotyl explants was investigated. Among the various concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP, Kn, Zn, and TDZ) tried, MT (Murashige and Tucker) medium fortified with benzylaminopurine (BAP) at 1 mg l⁻¹ without auxin had a strong promotive effect on shoot regeneration, and elucidated best morphogenic response from one-month-old etiolated epicotyl explants. A 100% regeneration frequency was obtained, and multiple shoots with an average of 8.24 shoots per explant were produced on all of the explants. Root formation was seen in response to all the three auxins viz. IBA, NAA and IAA, but the best response with rapid induction was observed under the influence of indole butyric acid (IBA) at 1 mg l⁻¹. Sucrose was observed to be at par with maltose as carbon source to support shoot regeneration. This study provided promising results, holds potential to be routinely employed for in vitro regeneration of important cultivars of Citrus spp, and can be incorporated for genetic transformation studies in citrus.
    Keywords Citrus sinensis ; carbon ; cultivars ; cytokinins ; etiolation ; explants ; genetic transformation ; in vitro regeneration ; indole acetic acid ; indole butyric acid ; maltose ; morphogenesis ; naphthaleneacetic acid ; oranges ; rooting ; shoots ; sucrose
    Language English
    Dates of publication 2010-07
    Size p. 195-202.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 2206337-7
    ISSN 0974-1275 ; 0971-7811
    ISSN (online) 0974-1275
    ISSN 0971-7811
    DOI 10.1007/BF03263340
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Citrus biotechnology: Achievements, limitations and future directions.

    Singh, Sandeepa / Rajam, Manchikatla V

    Physiology and molecular biology of plants : an international journal of functional plant biology

    2009  Volume 15, Issue 1, Page(s) 3–22

    Abstract: Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is ... ...

    Abstract Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.
    Language English
    Publishing date 2009-05-14
    Publishing country India
    Document type Journal Article
    ZDB-ID 2487126-6
    ISSN 0974-0430 ; 0971-5894
    ISSN (online) 0974-0430
    ISSN 0971-5894
    DOI 10.1007/s12298-009-0001-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Highly Efficient and Rapid Plant Regeneration in Citrus sinensis

    Singh, Sandeepa / Rajam, Manchikatla Venkat

    Journal of plant biochemistry and biotechnology

    Volume v. 19,, Issue no. 2

    Abstract: Sweet orange (Citrus sinensis L Osbeck, var Nagpur) was explored for efficient multiple shoot regeneration and rooting in different media. The influence of phytohormones and carbon source on the in vitro morphogenesis of sweet orange epicotyl explants ... ...

    Abstract Sweet orange (Citrus sinensis L Osbeck, var Nagpur) was explored for efficient multiple shoot regeneration and rooting in different media. The influence of phytohormones and carbon source on the in vitro morphogenesis of sweet orange epicotyl explants was investigated. Among the various concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP, Kn, Zn, and TDZ) tried, MT (Murashige and Tucker) medium fortified with benzylaminopurine (BAP) at 1 mg l⁻¹ without auxin had a strong promotive effect on shoot regeneration, and elucidated best morphogenic response from one-month-old etiolated epicotyl explants. A 100% regeneration frequency was obtained, and multiple shoots with an average of 8.24 shoots per explant were produced on all of the explants. Root formation was seen in response to all the three auxins viz. IBA, NAA and IAA, but the best response with rapid induction was observed under the influence of indole butyric acid (IBA) at 1 mg l⁻¹. Sucrose was observed to be at par with maltose as carbon source to support shoot regeneration. This study provided promising results, holds potential to be routinely employed for in vitro regeneration of important cultivars of Citrus spp, and can be incorporated for genetic transformation studies in citrus.
    Keywords rooting ; maltose ; in vitro regeneration ; indole butyric acid ; oranges ; indole acetic acid ; sucrose ; genetic transformation ; Citrus sinensis ; morphogenesis ; naphthaleneacetic acid ; carbon ; shoots ; etiolation ; cultivars ; cytokinins ; explants
    Language English
    Document type Article
    ISSN 0971-7811
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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