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Article ; Online: Assessment of microwave drying for rapid and convenient analysis of medicinal plants for quality assurance.

Bala, Esha / Dey, Saurav / Patra, Sanjukta / Singha, Siddhartha

2024

Abstract: Introduction: The safety and quality of many medicinally important herbs are compromised since farmers and small organizations are involved in the cultivation, aggregation, and primary processing of these herbs. Such organizations often lack adequate ... ...

Abstract	Introduction: The safety and quality of many medicinally important herbs are compromised since farmers and small organizations are involved in the cultivation, aggregation, and primary processing of these herbs. Such organizations often lack adequate quality control facilities. To improve the safety and quality of herbal products, simple, rapid, and affordable quality control systems are required. Objectives: The aim of this study was to assess the suitability of microwave oven-drying for moisture content (MC) determination and sample preparation of herbs in small organizations. Methods: Microwave oven-drying (720 W) and convective oven-drying at 105°C for MC determination were compared. The effects of three different drying methods (microwave oven-drying, low-temperature convective drying, and freeze-drying) on in vitro antioxidant and polyphenol oxidase (PPO) activity were determined, similarity analysis was conducted using HPLC signature spectra, and validation was performed with LC-MS focusing on one herb. Results: Microwave oven-drying at 720 W significantly reduced the drying time (from hours to minutes), whereas the spatial variation of temperature in convective ovens set at 105°C can cause about 10% underestimation of MC. Microwave oven-drying showed similar macro-properties like freeze-drying and higher extractability (10%-20%) and in vitro antioxidant capacity (33%-66%) and lower PPO activity compared to low-temperature convective drying. HPLC signature spectra revealed strong similarity of soluble components between freeze-dried and microwave oven-dried herbs. LC-MS analysis demonstrated more common compounds between freeze-dried and microwave oven-dried Centella asiatica extracts, whereas convective tray-dried samples had fewer compounds common with samples obtained by freeze-drying or microwave oven-drying. Conclusions: Microwave oven-drying is rapid (tens of min) and shows small batch-to-batch variation compared to oven-drying at 105°C. The in vitro antioxidant assays and signature spectra can be used for assessing the source and purity or quality of a specific herb variety.
Language	English
Publishing date	2024-02-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1073576-8
ISSN	1099-1565 ; 0958-0344
ISSN (online)	1099-1565
ISSN	0958-0344
DOI	10.1002/pca.3336
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Article ; Online: Cooperativity between Intermolecular Hydrogen and Carbon Bonds in ZY···CH

Gupta, Riya / Singha, Sujan / Mani, Devendra

The journal of physical chemistry. A

2024

Abstract: Hydrogen-bonding and carbon-bonding interactions are widespread in nature. We studied the cooperativity between these interactions in 42 trimeric complexes ZY··· ... ...

Abstract	Hydrogen-bonding and carbon-bonding interactions are widespread in nature. We studied the cooperativity between these interactions in 42 trimeric complexes ZY···CH
Language	English
Publishing date	2024-04-10
Publishing country	United States
Document type	Journal Article
ISSN	1520-5215
ISSN (online)	1520-5215
DOI	10.1021/acs.jpca.4c00911
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Article: Control of Japanese encephalitis by active principles, 3-(2-Hydroxyphenoxy)-1, 2-propanediol isolated from n-hexane extract of leaf of Mesua ferrea Linn

Singha, Someshwar

International journal of tropical insect science. 2021 June, v. 41, no. 2

2021

Abstract: Japanese encephalitis is a vector borne disease and the best procedure for control of this disease is control of vector population. Now a day use of phyto-chemicals more preferred over use of chemical insecticides. Isolation, purification and ... ...

Abstract	Japanese encephalitis is a vector borne disease and the best procedure for control of this disease is control of vector population. Now a day use of phyto-chemicals more preferred over use of chemical insecticides. Isolation, purification and determination of chemical structures of bioactive compounds were done successfully from n-hexane extract of leaf of M. ferrea. The compounds shows better potentiality to control Cx. vishnui 3rd instars larvae. n-hexane extract of leaf of M. ferrea was prepared by Sohxlet apparatus and further used for bioassay against 3rd instars larvae of Cx. vishnui. n-hexane extract was further fractioned by Thin layer chromatography to isolate bioactive compounds. From different band of TLC, bioactive compounds bearing band was isolated by bioassay test. Structures of active principles were determined by FTIR, gas chromatography, mass spectrometry and with the help of library data list. Cent percent mortality of Cx. vishnui larvae was recorded at 45 ppm concentration of n-hexane extract of M. ferrea after 72 h of exposure. In isolated bioactive compounds 3-(2-Hydroxyphenoxy)-1, 2-propanediol is one of predominant compound, may be responsible for mortality of Cx. vishnui larvae. LC₅₀ value for Cx. vishnui 3rd instars larvae of active compound are 26.76, 25.64 and 20.32 ppm for 24 h, 48 h and 72 h respectively. No mortality were recorded when sub lethal concentration (LC₅₀) of 24 h of bioactive compound applied on non-target organisms i.e. Chironomus circumdatus, Daphnia, Diplonychus etc. The compound i.e. 3-(2-Hydroxyphenoxy)-1, 2-propanediol is predominant compound of active principles and its chemical structure is determine which is major component of n-hexane extract of leaf of M. ferrea. These bioactive principles are safe for other organisms (non-target) those share the same natural habitat.
Keywords	Chironomus ; Daphnia ; Diplonychus ; Mesua ferrea ; active ingredients ; bioactive compounds ; bioassays ; chemical structure ; encephalitis ; gas chromatography ; habitats ; hexane ; insects ; instars ; leaves ; mass spectrometry ; mortality ; phytochemicals ; thin layer chromatography
Language	English
Dates of publication	2021-06
Size	p. 1061-1067.
Publishing place	Springer International Publishing
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2166668-4
ISSN	1742-7592 ; 1742-7584
ISSN (online)	1742-7592
ISSN	1742-7584
DOI	10.1007/s42690-020-00289-3
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Connectome-guided initiation of dopamine agonists facilitates cognitive recovery after frontal lobe resection: A case report.

Galvez, Rosivel / Singha, Souvik / Singer, Samuel / D'Amico, Randy S

Clinical neurology and neurosurgery

2024 Volume 237, Page(s) 108145

Abstract: Abulia is a common problem that manifests following various brain conditions, including brain surgeries. Abulia is felt to be related to dysfunction with the brain's dopamine-dependent circuitry. The role of default mode network (DMN) in its pathogenesis ...

Abstract	Abulia is a common problem that manifests following various brain conditions, including brain surgeries. Abulia is felt to be related to dysfunction with the brain's dopamine-dependent circuitry. The role of default mode network (DMN) in its pathogenesis is crucial. In this case report, we detail the presentation of abulia in an elderly woman following surgical resection of a right frontal glioblastoma involving the DMN. Connectomic imaging was used pre-operatively and post-operatively, demonstrating disruption of regions integral to the DMN and the central executive network. We observed a significant cognitive improvement following the administration of levodopa and carbidopa. Preoperative assessment of both anatomical and functional networks can help ensure surgical safety and predict postoperative deficits. This evaluation not only enhances preparedness and facilitates early case diagnosis but also expedites the initiation of prompt and potentially targeted treatments. This case highlights the potential efficacy of levodopa and carbidopa in addressing DMN dysfunction and broadly suggests the potential for connectomics-guided post-operative therapies.
MeSH term(s)	Female ; Humans ; Aged ; Connectome ; Brain/pathology ; Dopamine Agonists/therapeutic use ; Levodopa/therapeutic use ; Carbidopa/therapeutic use ; Magnetic Resonance Imaging ; Cognition ; Frontal Lobe/diagnostic imaging ; Frontal Lobe/surgery
Chemical Substances	Dopamine Agonists ; Levodopa (46627O600J) ; Carbidopa (MNX7R8C5VO)
Language	English
Publishing date	2024-01-28
Publishing country	Netherlands
Document type	Case Reports
ZDB-ID	193107-6
ISSN	1872-6968 ; 0303-8467
ISSN (online)	1872-6968
ISSN	0303-8467
DOI	10.1016/j.clineuro.2024.108145
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Article ; Online: Intentional Trapping of the Anterior Communicating Artery in a Case of Blister or Very Small (<3 mm) Aneurysm: Case Video with Imaging and Clinical Outcomes.

Singha, Souvik / Deora, Harsh / Rao, Malla Bhaskara

Neurology India

2023 Volume 71, Issue 3, Page(s) 435–438

MeSH term(s)	Humans ; Blister ; Anterior Cerebral Artery ; Aneurysm ; Intracranial Aneurysm/diagnostic imaging ; Intracranial Aneurysm/surgery ; Aneurysm, Ruptured
Language	English
Publishing date	2023-06-16
Publishing country	India
Document type	Case Reports ; Journal Article
ZDB-ID	415522-1
ISSN	1998-4022 ; 0028-3886
ISSN (online)	1998-4022
ISSN	0028-3886
DOI	10.4103/0028-3886.378711
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Book ; Online: Computational workflow for investigating hydrogen permeation in novel hydrogen storage materials

Singha, Sourabh / Chatterjee, Abhijit

2023

Abstract: The United States Department of Energy (DOE) has set ambitious targets for hydrogen storage materials for onboard light-duty cars which are to be achieved by 2027. One of the major problems in solid hydrogen storage materials is the sluggish uptake/ ... ...

Abstract	The United States Department of Energy (DOE) has set ambitious targets for hydrogen storage materials for onboard light-duty cars which are to be achieved by 2027. One of the major problems in solid hydrogen storage materials is the sluggish uptake/release kinetics. Much attention has been focused on understanding kinetics. Hydrogen solid-state diffusion is a rate-controlling step in the majority of metal hydrides. Here we dis-cuss computational workflow which can be used to estimate hydrogen diffusivity. A de-tailed study of hydrogen concentration, hydrogen neighbors is performed on nickel hy-dride (NiH) fcc materials to understand their effect on H diffusion. The nudged elastic band (NEB) method is used to determine hydrogen diffusion barrier with various hydro-gen concentrations in presence of hydrogen neighbors. The energy barriers for hydrogen hopping were calculated for few million different configurations with various local chemical environments. Two paths for H hopping from one octahedral site to a vacant neighbor octahedral site are identified: one path is straight, and the other is curved via tetrahedral site. The curved path shows diffusion faster than the straight path. This study demonstrates H diffusion is faster at higher hydrogen concentration, as the concomitant volume expansion lowers the energy barrier. Comment: 15 pages, 6 figures, 9th International Conference on Advances in Energy Research (ICAER)
Keywords	Condensed Matter - Materials Science
Subject code	541 ; 500
Publishing date	2023-12-09
Publishing country	us
Document type	Book ; Online
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Article: The diagnostic accuracy of preoperative perfusion index as a predictor of postspinal anesthesia hypotension in parturients undergoing cesarean delivery: A prospective non-blinded observational study.

Kesavankutty, Manu P / Panda, Chinmaya / Karim, Habib M R / Singha, Subrata / Agrawal, Sarita

Saudi journal of anaesthesia

2024 Volume 18, Issue 1, Page(s) 23–30

Abstract: Background and objectives: Spinal anesthesia is the technique of choice for elective cesarean section with a prominent side effect of postspinal anesthesia hypotension (PSH). This needs an early prediction to avoid feto-maternal complication. This study ...

Abstract	Background and objectives: Spinal anesthesia is the technique of choice for elective cesarean section with a prominent side effect of postspinal anesthesia hypotension (PSH). This needs an early prediction to avoid feto-maternal complication. This study aimed to assess the diagnostic accuracy of perfusion index (PI) and inferior vena cava collapsibility index (IVCCI) in the prediction of PSH. Material and methods: Thirty parturients of American Society of Anesthesiologists Physical Status (ASA-PS) 1 and two undergoing cesarean delivery participated in the study. IVCCI, PI, baseline systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MBP), and heart rate (HR) were noted in the preoperative period. The fall of MBP by 20% from baseline or below 65 mm Hg was considered PSH. After spinal anesthesia, SBP, DBP, MBP, and HR were noted again for diagnosing PSH. Results: It did not show any statistical difference when comparing the PI between the PSH and non-PSH groups in both the PSH definition groups. IVCCI was significantly higher when PSH was considered MBP <65 mm Hg ( Conclusion: Although there is a modest correlation between PI and IVCCI, both cannot be used to predict postspinal hypotension in parturients undergoing elective lower-segment cesarean section (LSCS).
Language	English
Publishing date	2024-01-02
Publishing country	India
Document type	Journal Article
ZDB-ID	2562174-9
ISSN	0975-3125 ; 1658-354X
ISSN (online)	0975-3125
ISSN	1658-354X
DOI	10.4103/sja.sja_378_23
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Article ; Online: Role of Optical Coherence Tomography in Predicting Visual Outcome after Surgery for Sellar and Supra-Sellar Tumors.

Singha, Souvik / Beniwal, Manish / Mailankody, Pooja / Battu, Rajani / Saini, Jitender / Tyagi, Gaurav / Srinivas, Dwarakanath

Neurology India

2024 Volume 72, Issue 1, Page(s) 50–57

Abstract: Background: Almost one-fifth of patients undergoing surgery for sellar/supra-sellar tumors do not gain a significant improvement in their vision. Various methods have been described to predict prospective visual outcomes in them, although they lack ... ...

Abstract	Background: Almost one-fifth of patients undergoing surgery for sellar/supra-sellar tumors do not gain a significant improvement in their vision. Various methods have been described to predict prospective visual outcomes in them, although they lack uniformity. Objective: The study was conducted to predict visual outcomes following surgery for sellar and supra-sellar tumors compressing the anterior optic pathway based on pre-operative optical coherence tomography (OCT) parameters. Methods and materials: This was a record-based observational descriptive longitudinal study done in a tertiary care center in India. Thirty-seven patients (74 eyes) diagnosed with sellar supra-sellar lesions were included in the study. Patients' ophthalmic evaluations, done pre-operatively and 3 months post-operatively, were reviewed. Spectral-domain OCT and segmentation were done using the automated segmentation technology of Spectralis software. The thickness of the respective layers was measured. Results and conclusions: The mean age of the study population was 42.68 years. Eyes with a pre-operative visual acuity component of VIS (visual impairment score) ≤61, pre-operative ganglion cell layer thickness ≥26.31 um, a pre-operative inner plexiform layer thickness of ≥25.69 um, a pre-operative ganglion cell inner plexiform layer thickness of 52.00 um, pre-operative ganglion cell complex thickness ≥84.47 μm, and a pre-operative inner retinal layer thickness of ≥205.25 μm were more likely to have an improved visual outcome. Eyes with a pre-operative duration of visual symptoms of ≥15 months, VIS ≥126.50, a pre-operative decimal visual acuity of <0.035, a pre-operative visual field index of ≤8%, a pre-operative macular thickness of ≤287.06 um, a pre-operative macular RNFL (retinal nerve fiber layer) thickness ≤66.00 μm, and a pre-operative peri-papillary RNFL thickness ≤64.62 μm were unlikely to have visual improvement.
MeSH term(s)	Humans ; Adult ; Tomography, Optical Coherence ; Longitudinal Studies ; Prospective Studies ; Skull Base Neoplasms ; Retina/diagnostic imaging ; Retina/surgery
Language	English
Publishing date	2024-02-29
Publishing country	India
Document type	Observational Study ; Journal Article
ZDB-ID	415522-1
ISSN	1998-4022 ; 0028-3886
ISSN (online)	1998-4022
ISSN	0028-3886
DOI	10.4103/neurol-india.Neurol-India-D-23-00654
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Article ; Online: HLA-G isoforms, HLA-C allotype and their expressions differ between early abortus and placenta in relation to spontaneous abortions.

Bora, Mayuri / Singha, Sushmita / Madan, Taruna / Deka, Gitanjali / Hazarika, Sumita Gogoi / Baruah, Shashi

Placenta

2024 Volume 149, Page(s) 44–53

Abstract: Introduction: Spontaneous abortion (SAB) affects approximately 10% of clinically recognized pregnancies. Fetal trophobalst invasion and remodeling of maternal spiral arteries is reported to be dependent on crosstalk between HLA-C/HLA-G expressed on ... ...

Abstract	Introduction: Spontaneous abortion (SAB) affects approximately 10% of clinically recognized pregnancies. Fetal trophobalst invasion and remodeling of maternal spiral arteries is reported to be dependent on crosstalk between HLA-C/HLA-G expressed on extra villous trophoblast (EVTs)and Killer cell Immunoglobin like receptors (KIRs) of decidual NK (dNK). Immune dysfunction in decidua contributes to early miscarriage. Methodology: The study used mother neonate paired cord blood and term placenta samples (n = 46), elective abortus (n = 17,gestational age = 10-12 weeks of pregnancy) and SAB abortus (n = 24, gestational age = 12-15 weeks of pregnancy) for HLA-G, KIR2D and HLA-C. In addition, term placenta was collected from women with history of spontaneous pregnancy loss (n = 24) and women with history of live birth (n = 32). SSP-PCR was used for genotyping, RT-PCR for gene expression, copy number variation (CNVs) and HLA-C allotyping and ELISA for protein expression studies. Results: Membrane bound HLA-G4 isoform proportion was higher 39.28%, p = 0.02) in term placenta. SAB abortus had higher proportion of HLA-G3 (50%),while elective abortus exhibited higher proportion of soluble isoforms (HLA-G5, = 5.9, HLA-G6 = 5.9%, HLA-G7 = 11.8%). Higher inhibitory KIR2DL1 content and copy numbers with lower HLA-C2 in SAB contrasted with higher copy numbers of KIR2DS1(p = 0.001), KIR2DS1 Conclusion: Our data supports lower cognate receptor ligand KIR2DS1+/2DL1+ HLA-C2 together with predominance of HLA-G3 isoform in SAB as confounding factors in spontaneous pregnancy loss. HLA-G isoforms and expression differed between first trimester abortus and term placenta suggesting temporal modulation and marks novelty of the study.
MeSH term(s)	Female ; Humans ; Infant ; Infant, Newborn ; Pregnancy ; Abortion, Spontaneous/genetics ; Abortion, Spontaneous/metabolism ; Decidua/metabolism ; DNA Copy Number Variations ; HLA-C Antigens/genetics ; HLA-G Antigens/genetics ; HLA-G Antigens/metabolism ; Killer Cells, Natural ; Placenta/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Trophoblasts/metabolism
Chemical Substances	HLA-C Antigens ; HLA-G Antigens ; Protein Isoforms
Language	English
Publishing date	2024-03-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	603951-0
ISSN	1532-3102 ; 0143-4004
ISSN (online)	1532-3102
ISSN	0143-4004
DOI	10.1016/j.placenta.2024.02.009
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Article ; Online: Capillary imbibition of confined monodisperse emulsions in microfluidic channels.

Norouzi Darabad, Masoud / Singha, Sagnik / Vanapalli, Siva A / Vaughn, Mark W / Blawzdziewicz, Jerzy

Soft matter

2024

Abstract: We study imbibition of a monodisperse emulsion into high-aspect ratio microfluidic channels with the ... ...

Abstract	We study imbibition of a monodisperse emulsion into high-aspect ratio microfluidic channels with the height
Language	English
Publishing date	2024-04-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2191476-X
ISSN	1744-6848 ; 1744-683X
ISSN (online)	1744-6848
ISSN	1744-683X
DOI	10.1039/d4sm00179f
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