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Article ; Online: A case series of the twiddler syndrome.

Montisci, Roberta / Soro, Cinzia / Demelas, Roberta / Agus, Elena / Follesa, Alessio / Siragusa, Gesualdo / Nissardi, Vincenzo

European heart journal. Case reports

2024 Volume 8, Issue 1, Page(s) ytae004

Abstract: Background: Twiddler syndrome (TS) is a complication of cardiac implantable electronic device (CIED) implantation, caused by the deliberate or unconscious manipulation of the device by the patient himself, which results in dislocation of the leads by ... ...

Abstract	Background: Twiddler syndrome (TS) is a complication of cardiac implantable electronic device (CIED) implantation, caused by the deliberate or unconscious manipulation of the device by the patient himself, which results in dislocation of the leads by retraction towards the subcutaneous pocket. Case summary: This report describes two clinical cases that occurred in our centre, for which two different solutions were successfully implemented. In the first case, a complete removal of the stimulation system was performed, and a leadless pacemaker (PM; Medtronic Micra VR) was implanted. In the second case, the patient underwent a revision procedure. The PM was disconnected, and the electrodes were debrided, a submuscular pocket for the PM was created, and at the end of the procedure, the PM was anchored to the pectoralis major. Discussion: Twiddler syndrome is a not so rare and serious complication of CIED implantation, leading to device malfunctioning and higher risk of infection of the pocket due to multiple re-interventions. In these two cases, different surgical solutions were performed, both resulting to be effective to solve the effects of TS.
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Case Reports
ISSN	2514-2119
ISSN (online)	2514-2119
DOI	10.1093/ehjcr/ytae004
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Article ; Online: Isolation of Listeria spp. from Feces of Feedlot Cattle.

Siragusa, G R / Dickson, J S / Daniels, E K

Journal of food protection

2019 Volume 56, Issue 2, Page(s) 102–105

Abstract: Healthy feedlot beef cattle were surveyed for the presence of Listeria spp. in fecal grab samples taken over 3 months. Composite samples were made from 224 individual animals each month. Listeria monocytogenes was isolated from one composite sample (4%) ... ...

Abstract	Healthy feedlot beef cattle were surveyed for the presence of Listeria spp. in fecal grab samples taken over 3 months. Composite samples were made from 224 individual animals each month. Listeria monocytogenes was isolated from one composite sample (4%) from the first sampling and not from the subsequent two. Listeria innocua was found in composite samples from all three samplings at levels of 17, 9, and 35%, respectively. From the individual samples comprising the Listeria spp.--positive composites, L. monoytogenes was isolated from one sample (3%) in the second sampling but not in the first or third samplings. L. innocua was found in 9, 8, and 10% of the individual samples comprising Listeria --positive composites in the first, second, and third samplings, respectively. The two L. monocytogenes isolates were pathogenic to mice. Further characterization of these isolates revealed atypical rhamnose fermentation patterns. These results indicate that the frequency of isolation of L. monocytogenes from feedlot beef cattle is low.
Language	English
Publishing date	2019-05-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	243284-5
ISSN	1944-9097 ; 0362-028X
ISSN (online)	1944-9097
ISSN	0362-028X
DOI	10.4315/0362-028X-56.2.102
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Article: Effects of Intraoperative Nerve Monitoring Techniques on Voice and Swallowing Disorders after Uncomplicated Thyroidectomy: Preliminary Report of a Bi-Institutional Prospective Study.

Melfa, Giuseppina / Siragusa, Giuseppe / Cocorullo, Gianfranco / Guzzino, Marianna / Raspanti, Cristina / Albanese, Leone / Mazzola, Sergio / Richiusa, Pierina / Orlando, Giuseppina / Scerrino, Gregorio

Journal of clinical medicine

2022 Volume 12, Issue 1

Abstract: Background: Voice and swallowing problems are frequently associated with thyroidectomy. Intermittent nerve monitoring (i-IONM) seems to provide a positive effect in reducing its prevalence. The aim of this study was to test the hypothesis that continuous ...

Abstract	Background: Voice and swallowing problems are frequently associated with thyroidectomy. Intermittent nerve monitoring (i-IONM) seems to provide a positive effect in reducing its prevalence. The aim of this study was to test the hypothesis that continuous intraoperative nerve monitoring (c-IONM) may reduce the prevalence of these disorders even further than i-IONM. Methods: This 3-arm prospective bi-institutional study compared 179 consecutive patients that underwent thyroidectomy: 56 without IONM, 55 with i-IONM and 67 with c-IONM. Neck dissections and laryngeal nerve palsies were excluded. Two questionnaires (VHI-10 for voice disorders and EAT-10 for swallowing disorders; both validated for Italian language use) were administered before and 1 month after surgery. Statistical significance was analyzed by the chi-squared test. Results: After thyroidectomy, no statistically significant differences were found in the three groups concerning EAT-10. although these symptoms seemed to be influenced by gastro-esophageal reflux. VHI-10 worsened in the “no-IONM” group compared with both i-IONM (p < 0.09, not quite statistically significant) and c-IONM (p < 0.04). Conclusion: Both i- and c-IONM improve voice quality independently of laryngeal nerve integrity. Reduced dissection and particularly restrained manipulation could explain these results, being particularly favorable for c-IONM.
Language	English
Publishing date	2022-12-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12010305
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Article ; Online: Antimicrobial Effects of Trisodium Phosphate Against Bacteria Attached to Beef Tissue.

Dickson, J S / Nettles Cutter, C G / Siragusa, G R

Journal of food protection

2019 Volume 57, Issue 11, Page(s) 952–955

Abstract: Sliced beef tissue was artificially contaminated with Salmonella typhimurium , Listeria monocytogenes and Escherichia coli O157:H7. The contaminated tissue was immersed in 8, 10 and 12% solutions of trisodium phosphate at 25, 40 and 55°C with contact ... ...

Abstract	Sliced beef tissue was artificially contaminated with Salmonella typhimurium , Listeria monocytogenes and Escherichia coli O157:H7. The contaminated tissue was immersed in 8, 10 and 12% solutions of trisodium phosphate at 25, 40 and 55°C with contact times of up to 3 min. The concentration of the trisodium phosphate solution was not a significant factor in reducing the populations of the bacteria on either lean or adipose tissue. Reductions in bacterial populations of 1 to 1.5 log
Language	English
Publishing date	2019-06-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	243284-5
ISSN	1944-9097 ; 0362-028X
ISSN (online)	1944-9097
ISSN	0362-028X
DOI	10.4315/0362-028X-57.11.952
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Article ; Online: Methods of Isolation, Characterization and Expansion of Human Adipose-Derived Stem Cells (ASCs): An Overview.

Palumbo, Paola / Lombardi, Francesca / Siragusa, Giuseppe / Cifone, Maria Grazia / Cinque, Benedetta / Giuliani, Maurizio

International journal of molecular sciences

2018 Volume 19, Issue 7

Abstract: Considering the increasing interest in adipose-derived stem cells (ASCs) in regenerative medicine, optimization of methods aimed at isolation, characterization, expansion and evaluation of differentiation potential is critical to ensure ( ...

Abstract	Considering the increasing interest in adipose-derived stem cells (ASCs) in regenerative medicine, optimization of methods aimed at isolation, characterization, expansion and evaluation of differentiation potential is critical to ensure (
MeSH term(s)	Adipocytes/cytology ; Adipose Tissue/cytology ; Cell Differentiation/genetics ; Cell Proliferation/genetics ; Cell Separation/methods ; Cell Separation/trends ; Humans ; Mesenchymal Stromal Cells/cytology ; Stem Cells/cytology
Language	English
Publishing date	2018-06-28
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms19071897
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Article: Quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets.

Wise, M G / Siragusa, G R

Journal of applied microbiology

2007 Volume 102, Issue 4, Page(s) 1138–1149

Abstract: Aims: To explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic ...

Abstract	Aims: To explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (AGPs) on a vegetable-based diet. Methods and results: Quantitative, real-time PCR with group-specific 16S rDNA primer sets was used to enumerate the abundance of the following chicken gastrointestinal (GI) tract phylogenetic groups: the Clostridium leptum-Faecalibacterium prausnitzii subgroup (Clostridium genus cluster IV), the Clostridium coccoides - Eubacterium rectale subgroup (Clostridium cluster XIVa and XIVb), the Bacteroides group (including Prevotella and Porphyromonas), Bifidobacterium spp., the Enterobacteriaceae, the Lactobacillus group (including the genera Leuconostoc, Pediococcus, Aerococcus and Weissella), the Clostridium perfringens subgroup (Clostridium cluster I), Enterococcus spp., Veillonella spp., Atopobium spp., Campylobacter spp. and the domain Bacteria. A species-specific 5'-nuclease (Taqman) assay was also employed to specifically assess Cl. perfringens abundance. Ten birds were sampled from each of two commercial chicken houses, one in which feed was supplemented with AGPs and exogenous animal protein, and the other vegetable-based and drug-free, at 7, 14 and 21 days of age. The ileal community was dominated by two large populations, the lactobacilli and the Enterobacteriaceae, with those taxa much more numerous in drug-free vegetable-based diet fed birds than those conventionally reared at the 7- and 14-day time periods. The progressive changes in microflora in both the conventional and drug-free caeca were similar to each other, with the Enterobacteriaceae sequences dominating at day 7, but being replaced by obligate anaerobe signature sequences by day 14. Of note was the finding that all the day 14 and day 21 replicate caecal samples from the drug-free house were positive for Campylobacter spp. averaging >10(8) 16S rDNA gene copies per gram wet weight. Conclusions: Quantitative, real-time PCR indicates that the effects of drug-free rearing on the chicken GI tract microbial community are most pronounced in the ileal region, but AGPs may be important in controlling Campylobacter colonization of the caecum. Significance and impact of the study: A quantitative taxonomic understanding of the shifting microbial ecology of the broiler chicken gut microbiota is important in the light of AGP withdrawal. AGP withdrawal has occurred in response to concerns over the transfer of antimicrobial-resistant bacteria to humans via the food production chain.
MeSH term(s)	Animal Feed/microbiology ; Animals ; Anti-Bacterial Agents ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Chickens/microbiology ; Gastrointestinal Tract/microbiology ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics ; Vegetables
Chemical Substances	Anti-Bacterial Agents ; RNA, Ribosomal, 16S
Language	English
Publishing date	2007-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/j.1365-2672.2006.03153.x
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Article ; Online: Involvement of NOS2 Activity on Human Glioma Cell Growth, Clonogenic Potential, and Neurosphere Generation.

Palumbo, Paola / Lombardi, Francesca / Siragusa, Giuseppe / Dehcordi, Soheila Raysi / Luzzi, Sabino / Cimini, AnnaMaria / Cifone, Maria Grazia / Cinque, Benedetta

International journal of molecular sciences

2018 Volume 19, Issue 9

Abstract: Aberrant nitric oxide synthase 2 (NOS2) expression has been suggested as an interesting therapeutic target that is being implicated as a component of the molecular profile of several human malignant tumors, including glioblastoma, which is the most ... ...

Abstract	Aberrant nitric oxide synthase 2 (NOS2) expression has been suggested as an interesting therapeutic target that is being implicated as a component of the molecular profile of several human malignant tumors, including glioblastoma, which is the most aggressive brain tumor with limited therapeutic options and poor prognosis. The aim of the present work was to evaluate the effect of 1400W, a specific NOS2 inhibitor, on human glioma cells in terms of clonogenic potential, proliferation, migration rate, and neurosphere generation ability. NOS2 expression was determined by Western blotting. Nitric oxide (NO) production was measured through nitrite level determination. The trypan blue exclusion test and the plate colony formation assay were performed to evaluate cell proliferation and clonogenic potential. Cell proliferation and migration ability was assessed by the
MeSH term(s)	Brain Neoplasms/metabolism ; Brain Neoplasms/pathology ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Glioblastoma/metabolism ; Glioblastoma/pathology ; Glioma/metabolism ; Glioma/pathology ; Humans ; Neoplasm Invasiveness/pathology ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/analysis ; Nitric Oxide Synthase Type II/metabolism ; Tumor Cells, Cultured
Chemical Substances	Nitric Oxide (31C4KY9ESH) ; NOS2 protein, human (EC 1.14.13.39) ; Nitric Oxide Synthase Type II (EC 1.14.13.39)
Language	English
Publishing date	2018-09-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms19092801
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Article: Official checks by an accredited laboratory on irradiated foods at an Italian market

Mangiacotti, M. / Marchesani, G. / Floridi, F. / Siragusa, G. / Chiaravalle, A. E.

Food control

2013 Volume 33, Issue 2, Page(s) 307

Document type	Article
ZDB-ID	1027805-9
ISSN	0956-7135
Database	Current Contents Nutrition, Environment, Agriculture

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Article ; Online: Multilocus sequence typing subtypes of poultry Clostridium perfringens isolates demonstrate disease niche partitioning.

Hibberd, M C / Neumann, A P / Rehberger, T G / Siragusa, G R

Journal of clinical microbiology

2011 Volume 49, Issue 4, Page(s) 1556–1567

Abstract: Clostridium perfringens is a ubiquitous and versatile pathogenic bacterium and is implicated in the etiology of the poultry diseases necrotic enteritis (NE) and poultry gangrene (PG). In this study, multilocus sequence typing was used to investigate ... ...

Abstract	Clostridium perfringens is a ubiquitous and versatile pathogenic bacterium and is implicated in the etiology of the poultry diseases necrotic enteritis (NE) and poultry gangrene (PG). In this study, multilocus sequence typing was used to investigate genotypic relationships among 139 C. perfringens isolates from 74 flocks. These isolates had multiple disease, host, and environmental origins. The results indicated a polymorphic yet highly clonal population, with 79.6% of all isolates partitioning into one of six clonal complexes or two dominant sequence types, ST-9 and ST-31. The most prolific clonal complex, CC-1, contained 27.3% of all isolates and was not clearly associated with one particular disease. The subtypes CC-4 and ST-31 were highly associated with NE and represented 9.4% and 7.2% of the total isolates, respectively. No PG-associated and NE-associated C. perfringens isolates shared the same sequence type or clonal complex. NE-associated subtypes were more clonal and appeared more evolutionarily divergent than PG-associated subtypes, which tended to cluster in the more ancestral lineages alongside isolates from asymptomatic chickens and turkeys. Toxin gene screening identified cpb2 throughout these isolates and correlated the presence of netB with NE pathology. Previous investigations into the genetic basis of C. perfringens pathogenicity have focused on toxins and other variable genetic elements. This study presents the first sequence-based comparison of C. perfringens isolates recovered in clinical cases of PG and NE and demonstrates that niche specialization is observable in the core genomes of poultry-associated C. perfringens isolates, a concept with both epidemiological and evolutionary significance.
MeSH term(s)	Animals ; Bacterial Typing Techniques ; Chickens ; Clostridium Infections/microbiology ; Clostridium Infections/veterinary ; Clostridium perfringens/classification ; Clostridium perfringens/genetics ; Clostridium perfringens/isolation & purification ; Cluster Analysis ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Genotype ; Molecular Sequence Data ; Multilocus Sequence Typing ; Poultry Diseases/microbiology ; Sequence Analysis, DNA ; Turkeys
Chemical Substances	DNA, Bacterial
Language	English
Publishing date	2011-01-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01884-10
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Zs.A 1188: Show issues

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Article: Production of bacteriocin inhibitory to Listeria species by Enterococcus hirae.

Siragusa, G R

Applied and environmental microbiology

1992 Volume 58, Issue 11, Page(s) 3508–3513

Abstract: A bovine intestinal bacterial isolate, identified as Enterococcus hirae, was found to produce a bacteriocin (designated hiraecin S) inhibitory to Listeria monocytogenes and other Listeria spp. Identification to species level was determined by ... ...

Abstract	A bovine intestinal bacterial isolate, identified as Enterococcus hirae, was found to produce a bacteriocin (designated hiraecin S) inhibitory to Listeria monocytogenes and other Listeria spp. Identification to species level was determined by comprehensive biochemical and morphological tests which were verified by DNA-DNA homology assays. The antimicrobial agent was inactivated by pronase and papain and was insensitive to catalase. The antimicrobial activity was not due to hydrogen peroxide or acid formation, nor was lysozyme or muramidase activity observed in cell-free bacteriocin preparations. Inhibition of selected gram-negative bacteria was not observed. Other enterococci were sensitive to the bacteriocin, and except for Listeria spp., no other gram-positive bacteria tested were inhibited.
MeSH term(s)	Animals ; Bacterial Typing Techniques ; Bacteriocins/isolation & purification ; Bacteriocins/pharmacology ; Cattle/microbiology ; Enterococcus/chemistry ; Listeria/drug effects ; Listeria monocytogenes/drug effects ; Microbial Sensitivity Tests
Chemical Substances	Bacteriocins
Language	English
Publishing date	1992-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.58.11.3508-3513.1992
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