Article ; Online: Applicability of a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection in high exposure risk setting.
2023 Volume 128, Page(s) 285–289
Abstract: Objectives: During the third wave, the growing number of COVID-19 case clusters reported countrywide in Thailand demonstrated the rapidly evolving characteristics of SARS-CoV-2, the causative agent of the COVID-19 pandemic. The rapid spread of COVID-19 ... ...
| Abstract | Objectives: During the third wave, the growing number of COVID-19 case clusters reported countrywide in Thailand demonstrated the rapidly evolving characteristics of SARS-CoV-2, the causative agent of the COVID-19 pandemic. The rapid spread of COVID-19 infections had been extensively reported in public areas and construction camps, as well as in congested communities with poor sanitation. High demand for SARS-CoV-2 genome testing and quick reporting by an hour for case identification and isolation characterizes the COVID-19 crisis in Thailand. This situation leads to an urgent need for alternative molecular tests which are reliable, rapid, and cost-effective. Methods: In this study, we assessed colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP), using real-time reverse transcription-polymerase chain reaction (RT-PCR) as a reference standard, for active case finding in suspected (mostly asymptomatic) cases living in high-risk areas of Bangkok. Results: The diagnostic performance of the RT-LAMP compared with real-time RT-PCR in specimens from 549 Thais were computed in a real-world field study setting. Our study demonstrated that RT-LAMP achieved robust identification of SARS-CoV-2 infection, with a diagnostic sensitivity and specificity of 91.67% and 100%, respectively. Conclusion: RT-LAMP is a reliable assay for SARS-CoV-2 detection and is scalable for use in the emergency response to a nationwide pandemic, despite resource limitations. The RT-LAMP real-world data derived from this field study validate its potential use in laboratory practice. RT-LAMP is a good choice as a laboratory-based SARS-CoV-2 molecular test when real-time RT-PCR is not available. |
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| MeSH term(s) | Humans ; SARS-CoV-2/genetics ; COVID-19 ; Reverse Transcription ; Pandemics ; Colorimetry ; Thailand ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Sensitivity and Specificity ; RNA, Viral/genetics |
| Chemical Substances | RNA, Viral |
| Language | English |
| Publishing date | 2023-01-13 |
| Publishing country | Canada |
| Document type | Journal Article |
| ZDB-ID | 1331197-9 |
| ISSN | 1878-3511 ; 1201-9712 |
| ISSN (online) | 1878-3511 |
| ISSN | 1201-9712 |
| DOI | 10.1016/j.ijid.2023.01.010 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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