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  1. Article ; Online: First study on in vitro antiviral and virucidal effects of flavonoids against feline infectious peritonitis virus at the early stage of infection

    Chanittha Triratapiban / Varanya Lueangaramkul / Nantawan Phecharat / Achiraya Pantanam / Porntippa Lekcharoensuk / Sirin Theerawatanasirikul

    Veterinary World, Vol 16, Iss 3, Pp 618-

    2023  Volume 630

    Abstract: Background and Aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, ... ...

    Abstract Background and Aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, with extremely high mortality in clinical FIP-infected cats, particularly young cats. There are no approved drugs for FIP treatment, and therapeutic possibilities for FIP treatment are limited. This study aimed to utilize nature-derived bioactive flavonoids with antiviral properties to inhibit FIPV infection in Crandell–Rees feline kidney (CRFK) cells. Materials and Methods: The cytotoxicity of 16 flavonoids was evaluated on CRFK cells using a colorimetric method (MTS) assay. Viral kinetics of FIPV at 50 tissue culture infectious dose (TCID50)/well was determined during the first 24-h post-infection (HPI). Antiviral activity was evaluated based on the replication steps of the virus life cycle, including pre-compound, attachment, penetration, post-viral entry, and virucidal assays. The antiviral efficacy of flavonoids against FIPV was determined based on positive FIPV-infected cells with the immunoperoxidase monolayer assay and viral load quantification using reverse transcription-quantitative polymerase chain reaction. Results: Two flavonoids, namely, isoginkgetin and luteolin, inhibited FIPV replication during post-viral entry in a dose-dependent manner, with 50% maximal effective concentrations = 4.77 ± 0.09 and 36.28 ± 0.03 μM, respectively. Based on viral kinetics, both flavonoids could inhibit FIPV replication at the early stage of infection at 0–6-HPI for isoginkgetin and 2–6-HPI for luteolin using a time-of-addition assay. Isoginkgetin exerted a direct virucidal effect that reduced the viral titers by 2 and 1.89 log10 TCID50/mL at 60 and 120 min, respectively. Conclusion: Isoginkgetin interfered with FIPV replication during both post-viral infection and virucidal experiments on CRFK cells, whereas luteolin inhibited the virus after infection. These results ...
    Keywords antiviral ; feline coronavirus ; feline infectious peritonitis virus ; flavonoids ; infectious disease ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
    Subject code 630 ; 570
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Veterinary World
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: The Application of the Gibson Assembly Method in the Production of Two pKLS3 Vector-Derived Infectious Clones of Foot-and-Mouth Disease Virus

    Ploypailin Semkum / Nattarat Thangthamniyom / Penpitcha Chankeeree / Challika Keawborisuth / Sirin Theerawatanasirikul / Porntippa Lekcharoensuk

    Vaccines, Vol 11, Iss 1111, p

    2023  Volume 1111

    Abstract: The construction of a full-length infectious clone, essential for molecular virological study and vaccine development, is quite a challenge for viruses with long genomes or possessing complex nucleotide sequence structures. Herein, we have constructed ... ...

    Abstract The construction of a full-length infectious clone, essential for molecular virological study and vaccine development, is quite a challenge for viruses with long genomes or possessing complex nucleotide sequence structures. Herein, we have constructed infectious clones of foot-and-mouth disease virus (FMDV) types O and A by joining each viral coding region with our pKLS3 vector in a single isothermal reaction using Gibson Assembly (GA). pKLS3 is a 4.3-kb FMDV minigenome. To achieve optimal conditions for the DNA joining, each FMDV coding sequence was divided into two overlapping fragments of approximately 3.8 and 3.2 kb, respectively. Both DNA fragments contain the introduced linker sequences for assembly with the linearized pKLS3 vector. FMDV infectious clones were produced upon directly transfecting the GA reaction into baby hamster kidney-21 (BHK-21) cells. After passing in BHK-21 cells, both rescued FMDVs (rO189 and rNP05) demonstrated growth kinetics and antigenicity similar to their parental viruses. Thus far, this is the first report on GA-derived, full-length infectious FMDV cDNA clones. This simple DNA assembly method and the FMDV minigenome would facilitate the construction of FMDV infectious clones and enable genetic manipulation for FMDV research and custom-made FMDV vaccine production.
    Keywords Gibson assembly ; foot-and-mouth disease virus ; reverse genetics ; vaccine ; infectious clone ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Andrographolide and Deoxyandrographolide Inhibit Protease and IFN-Antagonist Activities of Foot-and-Mouth Disease Virus 3C pro

    Sirin Theerawatanasirikul / Varanya Lueangaramkul / Nattarat Thangthamniyom / Penpitcha Chankeeree / Ploypailin Semkum / Porntippa Lekcharoensuk

    Animals, Vol 12, Iss 15, p

    2022  Volume 1995

    Abstract: Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment ... ...

    Abstract Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment available. Several studies have shown that plant-derived products with antiviral properties were effective on viral diseases. Herein, antiviral activities of andrographolide (AGL), deoxyandrographolide (DAG), and neoandrographolide (NEO) against FMDV serotype A were investigated using an in vitro cell-based assay. The results showed that AGL and DAG inhibited FMDV in BHK-21 cells. The inhibitory effects of AGL and DAG were evaluated by RT-qPCR and exhibited EC50 values of 52.18 ± 0.01 µM (SI = 2.23) and 36.47 ± 0.07 µM (SI = 9.22), respectively. The intracellular protease assay revealed that AGL and DAG inhibited FMDV 3C pro with IC50 of 67.43 ± 0.81 and 25.58 ± 1.41 µM, respectively. Additionally, AGL and DAG significantly interfered with interferon (IFN) antagonist activity of the 3C pro by derepressing interferon-stimulating gene (ISGs) expression. The molecular docking confirmed that the andrographolides preferentially interacted with the 3C pro active site. However, NEO had no antiviral effect in any of the assays. Conclusively, AGL and DAG inhibited FMDV serotype A by interacting with the 3C pro and hindered its protease and IFN antagonist activities.
    Keywords 3C protease (3C pro ) ; andrographolide ; antiviral activity ; deoxyandrographolide ; diterpenoids ; foot-and-mouth disease virus ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 333
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Breed-related number and size of muscle fibres and their response to carcass quality in chickens

    Nunyarat Koomkrong / Sirin Theerawatanasirikul / Chaiwat Boonkaewwan / Sanchai Jaturasitha / Autchara Kayan

    Italian Journal of Animal Science, Vol 14, Iss

    2015  Volume 4

    Abstract: The present study was aimed to investigate the number and size of muscle fibre and their relation to carcass quality traits in chickens (slow- and fast-growing chicken strains). A total of 40 one-day-old Arbor Acres broiler (fast-growing) and 40 Thai ... ...

    Abstract The present study was aimed to investigate the number and size of muscle fibre and their relation to carcass quality traits in chickens (slow- and fast-growing chicken strains). A total of 40 one-day-old Arbor Acres broiler (fast-growing) and 40 Thai native chickens (slow-growing) were reared to 45 and 112 days, respectively. The Arbor Acres broilers had heavier live weight, higher breast and thigh percentage than Thai native chickens (P<0.001). In breast muscle, there was no significant difference in total number of fibres and perimysium thickness. Thai native chickens had smaller fibre diameter and fibre area (P<0.01), and thicker endomysium in comparison with Arbor Acres broiler (P<0.001). The difference between the thigh and breast muscle fibre characteristics was not significant (P>0.05). The fibre diameter was positively correlated with live weight (P<0.05) and breast percentage (P<0.01). Endomysium thickness was correlated with live weight and breast percentage (P<0.05). There was no significant difference for the correlation between muscle fibre characteristics and thigh muscle. These results suggest that muscle fibre characteristics might be related to carcass quality.
    Keywords Carcass quality ; Muscle characteristic ; Muscle fibre ; Broiler ; Native chicken ; Animal culture ; SF1-1100 ; Agriculture ; S
    Subject code 621
    Language English
    Publishing date 2015-11-01T00:00:00Z
    Publisher PAGEPress Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Fiber characteristics of pork muscle exhibiting different levels of drip loss

    Koomkrong, Nunyarat / Nirat Gongruttananun / Chaiwat Boonkaewwan / Jatuporn Noosud / Sirin Theerawatanasirikul / Autchara Kayan

    Animal science journal =. 2017 Dec., v. 88, no. 12

    2017  

    Abstract: The present study aimed to investigate the fiber characteristics of pork muscle exhibiting different levels of drip loss during storage. The samples were taken from Longissimus dorsi muscle to evaluate drip loss (n = 100). Fifteen muscles per group (low ... ...

    Abstract The present study aimed to investigate the fiber characteristics of pork muscle exhibiting different levels of drip loss during storage. The samples were taken from Longissimus dorsi muscle to evaluate drip loss (n = 100). Fifteen muscles per group (low and high drip loss) were selected to evaluate the histological characteristics at 0 and 72 h of storage time. The statistical analysis revealed that a high drip loss group had greater endomysium thickness than a low drip loss group at 0 h of storage time (P < 0.05). There were no significant differences in total number of fibers, fiber diameter, fiber area and perimysium thickness at 0 h of storage time (P > 0.05). At 72 h of storage time, a high drip loss was evident in higher total number of fibers (P < 0.01), but smaller fiber diameter (P < 0.05), fiber area (P < 0.01) and endomysium thickness (P < 0.01) than a low drip loss group. There was no significant difference in perimysium thickness (P > 0.05). In conclusion, drip loss might be affected by muscle structural characteristics during storage.
    Keywords drip loss ; histology ; longissimus muscle ; pork ; statistical analysis ; storage time
    Language English
    Dates of publication 2017-12
    Size p. 2044-2049.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2095161-9
    ISSN 1740-0929 ; 1344-3941
    ISSN (online) 1740-0929
    ISSN 1344-3941
    DOI 10.1111/asj.12859
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Differential expression patterns of proteins involved in epidermal proliferation and differentiation in canine atopic dermatitis

    Sirin Theerawatanasirikul(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology) / Achariya Sailasuta(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology) / Roongroje Thanawongnuwech(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology) / Tossaporn Nakbed(Charoennakorn Vet Clinic, Bangkok (Thailand)) / Komkrid Charngkaew(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology) / Gunnaporn Suriyaphol(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Physiology. Biochemistry Unit) E-mail:gunnaporn.v@chula.ac.th

    Wetchasan Sattawaphaet

    Sep 2012  , Issue (3)

    Keywords Growth ; Skin ; Pathogenicity ; Cells ; Dogs ; Histopathology ; Proteins ; Dermatitis
    Language English
    Document type Article
    ISSN 0125-6491
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  7. Article: Association of filaggrin (FLG) gene polymorphism with canine atopic dermatitis in small breed dogs

    Gunnaporn Suriyaphol(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Physiology. Biochemistry Unit) E-mail:Gunnaporn.V@chula.ac.th / Prapat Suriyaphol(Mahidol University, Bangkok (Thailand). Faculty of Medicine. Siriraj Hospital. Office for Research and Development. Bioinformatics and Data Management for Research Unit) / Meena Sarikaputi(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Physiology. Biochemistry Unit) / Sirin Theerawatanasirikul(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology. Pathology Unit) / Achariya Sailasuta(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Veterinary Pathology. Pathology Unit)

    Wetchasan Sattawaphaet

    Dec 2011  , Issue (4)

    Keywords Genes ; Skin diseases ; Polymorphism ; Dogs ; Dermatitis
    Language English
    Document type Article
    ISSN 0125-6491
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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