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  1. Article: The analysis of

    Frąszczak, Karolina / Barczyński, Bartłomiej / Siwiec, Radosław / Kondracka, Adrianna / Malm, Anna / Kotarski, Jan / Witt, Elzbieta / Korona-Głowniak, Izabela

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1257587

    Abstract: Introduction: A healthy vaginal microbiota is represented mainly by : Methods: The study included 100 women (79 patients with abnormal cervical Pap smear results and 21 controls) from whom vaginal swabs were collected. Real-time quantitative PCR was ... ...

    Abstract Introduction: A healthy vaginal microbiota is represented mainly by
    Methods: The study included 100 women (79 patients with abnormal cervical Pap smear results and 21 controls) from whom vaginal swabs were collected. Real-time quantitative PCR was used to determine seven lactic acid bacteria (LAB) species and their quantities.
    Results: Most patients were colonized by two
    Discussion: The degree of dysplastic changes in the endothelium or the presence of a group of atypical cervical stratified epithelial cells was not associated with significant changes in the studied vaginal bacteria.
    Language English
    Publishing date 2023-11-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1257587
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  2. Article ; Online: Microbiological evaluation of 10 commercial probiotic products available in Poland

    Korona-Glowniak Izabela / Siwiec Radoslaw / Luszczewska-Sierakowska Iwona / Maciejewski Ryszard / Wrobel Rafal / Malm Anna

    Current Issues in Pharmacy and Medical Sciences, Vol 32, Iss 3, Pp 121-

    2019  Volume 124

    Abstract: The aim of this study was to analyze the quality of 10 commonly available commercial probiotic products used in Poland. These items were tested for the total viable bacterial count, and for identifying the isolated strains. This was performed using the ... ...

    Abstract The aim of this study was to analyze the quality of 10 commonly available commercial probiotic products used in Poland. These items were tested for the total viable bacterial count, and for identifying the isolated strains. This was performed using the Polymerase Chain Reaction method. The results showed that five of the tested products had not the applicable number of viable bacteria declared by manufacturer. Moreover, not all declared probiotic strains were found in three of the tested products during analyses. It is clear that a regular control of probiotic products needs be introduced that can guarantee its beneficial properties.
    Keywords probiotics ; viable bacterial count ; lactobacilli ; bifidobacteria ; Medicine ; R
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher Sciendo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Resistance determinants and their association with different transposons in the antibiotic-resistant Streptococcus pneumoniae.

    Korona-Glowniak, Izabela / Siwiec, Radoslaw / Malm, Anna

    BioMed research international

    2015  Volume 2015, Page(s) 836496

    Abstract: Multiple resistance of Streptococcus pneumoniae is generally associated with their unique recombination-mediated genetic plasticity and possessing the mobile genetic elements. The aim of our study was to detect antibiotic resistance determinants and ... ...

    Abstract Multiple resistance of Streptococcus pneumoniae is generally associated with their unique recombination-mediated genetic plasticity and possessing the mobile genetic elements. The aim of our study was to detect antibiotic resistance determinants and conjugative transposons in 138 antibiotic-resistant pneumococcal strains isolated from nasopharynx of healthy young children from Lublin, Poland. These strains resistant to tetracycline and/or to chloramphenicol/erythromycin/clindamycin were tested by PCR using the specific genes as markers. The presence of Tn916 family transposons, carrying tet(M) and int/xisTn916, was observed in all of the tested strains. Tn916 was detected in 16 strains resistant only to tetracycline. Tn6002 and Tn3872-related element were found among 99 erm(B)-carrying strains (83.8% and 3.0%, resp.). Eight strains harbouring mef(E) and erm(B) genes were detected, suggesting the presence of Tn2010 and Tn2017 transposons. Among 101 chloramphenicol-resistant strains, two variants of Tn5252-related transposon were distinguished depending on the presence of int/xis5252 genes specific for cat gene-containing Tn5252 (75.2% of strains) or int Sp23FST81 gene, specific for cat-containing ICESp23FST81 element (24.8% of strains). In 6 strains Tn916-like and Tn5252-like elements formed a Tn5253-like structure. Besides clonal dissemination of resistant strains of pneumococci in the population, horizontal transfer of conjugative transposons is an important factor of the high prevalence of antibiotic resistance.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; DNA Transposable Elements/genetics ; Drug Resistance, Bacterial/genetics ; Streptococcus pneumoniae/genetics
    Chemical Substances Anti-Bacterial Agents ; DNA Transposable Elements
    Language English
    Publishing date 2015
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2015/836496
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  4. Article ; Online: Resistant Streptococcus pneumoniae strains in children with acute otitis media- high risk of persistent colonization after treatment.

    Korona-Glowniak, Izabela / Zychowski, Piotr / Siwiec, Radoslaw / Mazur, Elżbieta / Niedzielska, Grażyna / Malm, Anna

    BMC infectious diseases

    2018  Volume 18, Issue 1, Page(s) 478

    Abstract: Background: Despite advances in the development of pneumococcal conjugate vaccines, acute otitis media (AOM) is a common childhood infection, caused mainly by Streptococcus pneumoniae. It has been suggested that persistence of pneumococcal ... ...

    Abstract Background: Despite advances in the development of pneumococcal conjugate vaccines, acute otitis media (AOM) is a common childhood infection, caused mainly by Streptococcus pneumoniae. It has been suggested that persistence of pneumococcal nasopharyngeal carriage is a risk factor for subsequent recurrent infections.
    Methods: In this study we evaluate the relationship between 55 pneumococcal strains obtained from nasopharynx/oropharynx (NP/OP) and middle ear fluid (MEF) of 62 children, aged between 1 and 16 years, during AOM (including recurrent/treatment failure AOM, and post-treatment visits), based on their phenotypic and genotypic characteristics performed by analyses of serotype, antibiotic susceptibility patterns and multilocus sequence typing.
    Results: S.pneumoniae was isolated from 27.4% of MEF samples; it constituted 43.6% of all positive bacterial samples from MEF samples. There was statistically significant concordance between isolation from the MEF sample and NP/OP colonization by S. pneumoniae (p < 0.0001). During post-treatment visits S.pneumoniae was isolated from 20.8% of children; 91% of them were positive in pneumococcal NP/OP culture during AOM. The serotypes belonging to 10- and 13-valent pneumococcal conjugated vaccines constituted 84% and 92% of the strains, respectively. Multidrug resistance was found in 84% of the strains. According to multivariate analysis, pneumococcal colonization after antibiotic therapy was significantly associated with shorter length of therapy in children with bilateral AOM.
    Conclusions: High persistent prevalence of antibiotic-resistant S.pneumoniae strains in children with AOM after unsuccessful bacterial eradication may presumably be regarded as a predisposing factor of infection recurrence.
    MeSH term(s) Acute Disease ; Adolescent ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Child ; Child, Preschool ; Drug Resistance, Multiple, Bacterial/drug effects ; Female ; Genotype ; Humans ; Infant ; Male ; Nasopharynx/microbiology ; Odds Ratio ; Oropharynx/microbiology ; Otitis Media/diagnosis ; Otitis Media/drug therapy ; Otitis Media/microbiology ; Pneumococcal Infections/diagnosis ; Pneumococcal Infections/drug therapy ; Pneumococcal Infections/microbiology ; Prospective Studies ; Serogroup ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/isolation & purification
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2018-09-25
    Publishing country England
    Document type Journal Article
    ISSN 1471-2334
    ISSN (online) 1471-2334
    DOI 10.1186/s12879-018-3398-9
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  5. Article ; Online: Molecular diagnostics of periodontitis.

    Korona-Głowniak, Izabela / Siwiec, Radosław / Berger, Marcin / Malm, Anna / Szymańska, Jolanta

    Postepy higieny i medycyny doswiadczalnej (Online)

    2017  Volume 71, Page(s) 47–56

    Abstract: The microorganisms that form dental plaque are the main cause of periodontitis. Their identification and the understanding of the complex relationships and interactions that involve these microorganisms, environmental factors and the host's health status ...

    Abstract The microorganisms that form dental plaque are the main cause of periodontitis. Their identification and the understanding of the complex relationships and interactions that involve these microorganisms, environmental factors and the host's health status enable improvement in diagnostics and targeted therapy in patients with periodontitis. To this end, molecular diagnostics techniques (both techniques based on the polymerase chain reaction and those involving nucleic acid analysis via hybridization) come increasingly into use. On the basis of a literature review, the following methods are presented: polymerase chain reaction (PCR), real-time polymerase chain reaction (real-time PCR), 16S rRNA-encoding gene sequencing, checkerboard and reverse-capture checkerboard hybridization, microarrays, denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), as well as terminal restriction fragment length polymorphism (TRFLP) and next generation sequencing (NGS). The advantages and drawbacks of each method in the examination of periopathogens are indicated. The techniques listed above allow fast detection of even small quantities of pathogen present in diagnostic material and prove particularly useful to detect microorganisms that are difficult or impossible to grow in a laboratory.
    MeSH term(s) DNA, Bacterial/genetics ; Dental Plaque/genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Pathology, Molecular ; Periodontitis/diagnosis ; Periodontitis/genetics ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA/methods
    Chemical Substances DNA, Bacterial ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2017-01-28
    Publishing country Poland
    Document type Journal Article ; Review
    ZDB-ID 418865-2
    ISSN 1732-2693 ; 0032-5449
    ISSN (online) 1732-2693
    ISSN 0032-5449
    DOI 10.5604/17322693.1229820
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  6. Article: Antibiotic Resistance and Genotypes of

    Korona-Glowniak, Izabela / Cichoz-Lach, Halina / Siwiec, Radoslaw / Andrzejczuk, Sylwia / Glowniak, Andrzej / Matras, Przemyslaw / Malm, Anna

    Journal of clinical medicine

    2019  Volume 8, Issue 7

    Abstract: The aim of this study was to investigate genetic diversity ... ...

    Abstract The aim of this study was to investigate genetic diversity of
    Language English
    Publishing date 2019-07-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm8071071
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  7. Article ; Online: Molecular Epidemiology of Streptococcus pneumoniae Isolates from Children with Recurrent Upper Respiratory Tract Infections.

    Korona-Glowniak, Izabela / Maj, Maciej / Siwiec, Radosław / Niedzielski, Artur / Malm, Anna

    PloS one

    2016  Volume 11, Issue 7, Page(s) e0158909

    Abstract: A total of 125 isolates were recovered from adenoids and/or nasopharynx of 170 children aged 2 to 5 from south-east Poland; they had undergone adenoidectomy for recurrent and/or persistent symptoms of upper respiratory tract infections. Pneumococcal ... ...

    Abstract A total of 125 isolates were recovered from adenoids and/or nasopharynx of 170 children aged 2 to 5 from south-east Poland; they had undergone adenoidectomy for recurrent and/or persistent symptoms of upper respiratory tract infections. Pneumococcal isolates were analyzed by phenotyping (serotyping and antimicrobial resistance tests) and genotyping together with the clonality of the pneumococcal isolates based on resistance determinants, transposon distribution and multilocus sequence typing (MLST). Serotypes 19F, 6B and 23F constituted 44.8% of the isolates. Among all of the strains, 44.8% showed decreased susceptibility to penicillin and resistance to co-trimoxazole (52.8%), tetracycline (38.4%), erythromycin (53.6%), clindamycin (52.8%) and chloramphenicol (27.2%) was observed. Tn6002 was found in 34.8% of erythromycin-resistant isolates while composite Tn2010-in 16.7% of erm(B)-carrying isolates that harboured also mef(E) gene. Tn3872-related elements were detected in 27.3% of erythromycin-resistant strains. In the majority of chloramphenicol-resistant catpC194-carrying isolates (79.4%), ICESp23FST81-family elements were detected. The genotyping showed that pneumococcal population was very heterogeneous; 82 sequence types (STs) were identified, and the most frequent contributed to not more than 8% of the isolates. Nearly 44% STs were novel, each of them was recovered only from one child. Four STs belonged to one of the 43 worldwide spread resistant pneumococcal clones currently accepted by Pneumococcal Molecular Epidemiology Network (PMEN), i.e. Spain 9V-3, Spain 23F-1, Norway NT-42 and Poland 6B-20, accounting for 12 (16.7%) of the 75 nonususceptible isolates, and five STs were single-locus variants of PMEN resistant clones (England 14-9, Spain 9V-3, Spain 23F-1, Greece 21-30, Denmark 14-32), accounting 9 (12%) of nonsusceptible isolates. A few MDR clones belonging to 6B and 19F serotypes found among preschool children emphasizes rather the role of clonal dissemination of local strains in the community than international clones spreading in the increase of resistance among pneumococcal strains.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Child, Preschool ; DNA Transposable Elements/genetics ; Drug Resistance, Bacterial/genetics ; Genes, Bacterial/genetics ; Humans ; Microbial Sensitivity Tests ; Molecular Epidemiology ; Multilocus Sequence Typing ; Pneumonia, Pneumococcal/drug therapy ; Pneumonia, Pneumococcal/epidemiology ; Poland/epidemiology ; Polymerase Chain Reaction ; Recurrence ; Respiratory Tract Infections/drug therapy ; Respiratory Tract Infections/epidemiology ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/genetics
    Chemical Substances Anti-Bacterial Agents ; DNA Transposable Elements
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0158909
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  8. Article: Fluoroquinolone-resistance mechanisms and phylogenetic background of clinical Escherichia coli strains isolated in south-east Poland.

    Korona-Glowniak, Izabela / Skrzypek, Kinga / Siwiec, Radosław / Wrobel, Andrzej / Malm, Anna

    The new microbiologica

    2016  Volume 39, Issue 3, Page(s) 210–215

    Abstract: Fluorochinolones are a class of broad-spectrum antimicrobials in the treatment of several infections, including those caused by Escherichia coli. Due to the increasing resistance of bacteria to antimicrobials, an understanding of fluoroquinolone ... ...

    Abstract Fluorochinolones are a class of broad-spectrum antimicrobials in the treatment of several infections, including those caused by Escherichia coli. Due to the increasing resistance of bacteria to antimicrobials, an understanding of fluoroquinolone resistance is important for infection control. The aim of this study was to determine susceptibility of clinical E. coli strains to fluoroquinolones and characterize their mechanisms of quinolone resistance. Totally, 79 non-duplicate clinical E. coli isolates included in this study were mainly from skin lesion -36 (45.6%) isolates; 54 (68.4%) isolates were assigned to phylogenetic B2 group. Resistance to ciprofloxacin was found in 20 isolates. In the quinolone resistance-determining region (QRDR) region of gyrA and parC, 4 types of point mutations were detected. Mutations in parC gene were found in all strains with gyrA mutations. Predominance of double mutation in codon 83 and 87 of gyrA (90%) and in codon 80 of parC (90%) was found. Moreover, plasmid-mediated quinolone resistance (PMRQ) determinants (qnrA or qnrB and/or aac(6')-Ib-cr) were present in 5 (25%) out of 20 fluoroquinolone-resistant isolates. Resistance to fluoroquinolones in all of the tested clinical E. coli isolates correlated with point mutations in both gyrA and parC. The majority of fluoroquinolone-resistant strains belonged to D and B2 phylogenetic groups.
    MeSH term(s) Anti-Bacterial Agents/classification ; Anti-Bacterial Agents/pharmacology ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Fluoroquinolones/pharmacology ; Humans ; Phylogeny ; Poland/epidemiology
    Chemical Substances Anti-Bacterial Agents ; Fluoroquinolones
    Language English
    Publishing date 2016-07
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 756168-4
    ISSN 1121-7138 ; 0391-5352
    ISSN 1121-7138 ; 0391-5352
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