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  1. Article ; Online: Transcriptome analysis in mouse skin after exposure to ultraviolet radiation from a canopy sunbed.

    Qutob, Sami S / Roesch, Samantha P M / Smiley, Sandy / Bellier, Pascale / Williams, Andrew / Cook, Kate B / Meier, Matthew J / Rowan-Carroll, Andrea / Yauk, Carole L / McNamee, James P

    Photochemistry and photobiology

    2024  

    Abstract: Exposure to ultraviolet radiation (UV-R), from both natural and artificial tanning, heightens the risk of skin cancer by inducing molecular changes in cells and tissues. Despite established transcriptional alterations at a molecular level due to UV-R ... ...

    Abstract Exposure to ultraviolet radiation (UV-R), from both natural and artificial tanning, heightens the risk of skin cancer by inducing molecular changes in cells and tissues. Despite established transcriptional alterations at a molecular level due to UV-R exposure, uncertainties persist regarding UV radiation characterization and subsequent genomic changes. Our study aimed to mechanistically explore dose- and time-dependent gene expression changes, that may drive short-term (e.g., sunburn) and long-term actinic (e.g., skin cancer) consequences. Using C57BL/6N mouse skin, we analyzed transcriptomic expression following exposure to five erythemally weighted UV-R doses (0, 5, 10, 20, and 40 mJ/cm
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 123540-0
    ISSN 1751-1097 ; 0031-8655
    ISSN (online) 1751-1097
    ISSN 0031-8655
    DOI 10.1111/php.13917
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Novel identification of matrix proteins involved in calcitic biomineralization.

    Rose-Martel, Megan / Smiley, Sandy / Hincke, Maxwell T

    Journal of proteomics

    2015  Volume 116, Page(s) 81–96

    Abstract: Calcitic biomineralization is essential for otoconia formation in vertebrates. This process is characterized by protein-crystal interactions that modulate crystal growth on an extracellular matrix. An excellent model for the study of calcitic ... ...

    Abstract Calcitic biomineralization is essential for otoconia formation in vertebrates. This process is characterized by protein-crystal interactions that modulate crystal growth on an extracellular matrix. An excellent model for the study of calcitic biomineralization is the avian eggshell, the fastest known biomineralization process. The objective of this study is to identify and characterize matrix proteins associated with the eggshell mammillary cones, which are hypothesized to regulate the earliest stage of eggshell calcification. Mammillary cones were isolated from 2 models, fertilized and unfertilized, and the released proteins were identified by RP-nanoLC and ES-MS/MS proteomics. Proteomics analysis identified 49 proteins associated with the eggshell membrane fibers and, importantly, 18 mammillary cone-specific proteins with an additional 18 proteins identified as enriched in the mammillary cones. Among the most promising candidates for modulating protein-crystal interactions were extracellular matrix proteins, including ABI family member 3 (NESH) binding protein (ABI3BP), tiarin-like, hyaluronan and proteoglycan link protein 3 (HAPLN3), collagen alpha-1(X), collagen alpha-1(II) and fibronectin, in addition to the calcium binding proteins calumenin, EGF-like repeats and discoidin 1-like domains 3 (EDIL3), nucleobindin-2 and SPARC. In conclusion, we identified several cone-resident proteins that are candidates to regulate initiation of eggshell calcification. Further study of these proteins will determine their roles in modulating calcitic biomineralization and lead to insight into the process of otoconia formation/regeneration.
    Biological significance: Biomineralization is essential for the development of hard tissues in vertebrates, which includes both calcium phosphate and calcium carbonate structures. Calcitic mineralization by calcium carbonate is an important process in the formation of otoconia, which are gravity receptor organs located in the inner ear and are responsible for balance and for sensing linear acceleration. Deficiencies in the regulation of their biomineralization can lead to otoconia degeneration and eventually benign paroxysmal position vertigo (BPPV), which is the main cause of vertigo in humans. Eggshell formation in chicken is one of the fastest known biomineralization processes and is an excellent model for the study of calcitic biomineralization. Cross-analysis of proteomic data from two mineralized models, fertilized and unfertilized chicken eggshells, identified proteins associated with the mammillary cones that are the sites of initiation of eggshell formation. We hypothesize that these proteins regulate the earliest stages of eggshell calcification. The human homologs of these proteins are therefore potential candidates to play a role in otoconia biomineralization.
    MeSH term(s) Animals ; Calcification, Physiologic/physiology ; Chickens ; Egg Proteins/metabolism ; Humans ; Proteome/metabolism ; Proteomics ; Zygote/metabolism
    Chemical Substances Egg Proteins ; Proteome ; chorion proteins
    Language English
    Publishing date 2015-01-10
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2400835-7
    ISSN 1876-7737 ; 1874-3919
    ISSN (online) 1876-7737
    ISSN 1874-3919
    DOI 10.1016/j.jprot.2015.01.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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