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  1. Article ; Online: Accelerated Hemocompatibility Testing of Rotary Blood Pumps.

    McNamee, Antony P / Griffith, Tia A / Smith, Amanda G / Kuck, Lennart / Simmonds, Michael J

    ASAIO journal (American Society for Artificial Internal Organs : 1992)

    2023  Volume 69, Issue 10, Page(s) 918–923

    Abstract: Ex vivo hemocompatibility testing is a vital element of preclinical assessment for blood-contacting medical devices. Current approaches are resource intensive; thus, we investigated the feasibility of accelerating hemocompatibility testing by ... ...

    Abstract Ex vivo hemocompatibility testing is a vital element of preclinical assessment for blood-contacting medical devices. Current approaches are resource intensive; thus, we investigated the feasibility of accelerating hemocompatibility testing by standardizing the number of pump exposures in loops of various sizes. Three identical blood loops were constructed, each with a custom-molded reservoir able to facilitate large-volume expansion. Using the HVAD rotary blood pump operating at 5 L·min -1 and 100 mmHg, three test volumes (80, 160, and 320 ml) were circulated for 4000 pump exposures. Blood sampling was performed at individualized intervals every one-sixth of total duration for the assessment of hemolysis and von Willebrand Factor (vWF) degradation. While steady increases in hemolysis (~24 mg·dl -1 ) were identified in all tests at completion, loop volume was not a primary discriminator. The normalized index of hemolysis did not vary significantly between loops (4.2-4.9 mg·100 L -1 ). vWF degradation progressively occurred with duration of testing to a similar extent under all conditions. These data support an accelerated approach to preclinical assessment of ex vivo blood damage. Adopting this approach enables: enhanced efficiency for rapid prototyping; reduced ex vivo blood aging, and; greater utility of blood, which is presently limited if 450 ml loops are desired.
    MeSH term(s) Humans ; von Willebrand Factor/metabolism ; Heart-Assist Devices/adverse effects ; Hemolysis ; Materials Testing ; Stress, Mechanical
    Chemical Substances von Willebrand Factor
    Language English
    Publishing date 2023-09-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 759982-1
    ISSN 1538-943X ; 0162-1432 ; 1058-2916
    ISSN (online) 1538-943X
    ISSN 0162-1432 ; 1058-2916
    DOI 10.1097/MAT.0000000000001995
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Clinical Trials, Potential Mechanisms, and Adverse Effects of Arnica as an Adjunct Medication for Pain Management.

    Smith, Amanda G / Miles, Victoria N / Holmes, Deltrice T / Chen, Xin / Lei, Wei

    Medicines (Basel, Switzerland)

    2021  Volume 8, Issue 10

    Abstract: Arnica has traditionally been used in treating numerous medical conditions, including inflammation and pain. This review aims to summarize the results of studies testing Arnica products for pain management under different conditions, including post- ... ...

    Abstract Arnica has traditionally been used in treating numerous medical conditions, including inflammation and pain. This review aims to summarize the results of studies testing Arnica products for pain management under different conditions, including post-operation, arthritis, low back pain, and other types of musculoskeletal pain. Based on data from clinical trials, Arnica extract or gel/cream containing Arnica extract shows promising effects for pain relief. These medical benefits of Arnica may be attributed to its chemical components, with demonstrated anti-inflammatory, antioxidant, anti-microbial, and other biological activities. In conclusion, Arnica could be an adjunct therapeutical approach for acute and chronic pain management.
    Language English
    Publishing date 2021-10-09
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2777965-8
    ISSN 2305-6320
    ISSN 2305-6320
    DOI 10.3390/medicines8100058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Behavioral problems in dementia. Strategies for pharmacologic and nonpharmacologic management.

    Smith, Amanda G

    Postgraduate medicine

    2004  Volume 115, Issue 6, Page(s) 47–52, 55–6

    Abstract: Behavioral disturbances in Alzheimer's disease and related disorders are common throughout all stages of these dementing illnesses. They are a major source of caregiver distress and can lead to premature institutionalization of the patient if not ... ...

    Abstract Behavioral disturbances in Alzheimer's disease and related disorders are common throughout all stages of these dementing illnesses. They are a major source of caregiver distress and can lead to premature institutionalization of the patient if not properly addressed. Fortunately, such behaviors are often treatable. In this article, Dr Smith outlines ways to manage behavioral problems in dementia with pharmacologic as well as nonpharmacologic approaches. Throughout, she emphasizes that proper treatment of problem behaviors in this vulnerable population requires the education and patience of caregivers and physicians alike.
    MeSH term(s) Aged ; Dementia/complications ; Dementia/drug therapy ; Humans ; Mental Disorders/drug therapy ; Mental Disorders/etiology ; Psychomotor Agitation/drug therapy ; Psychomotor Agitation/etiology ; Sleep Wake Disorders/drug therapy ; Sleep Wake Disorders/etiology
    Language English
    Publishing date 2004-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 410138-8
    ISSN 1941-9260 ; 0032-5481
    ISSN (online) 1941-9260
    ISSN 0032-5481
    DOI 10.3810/pgm.2004.06.1536
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The Effect of Heat Shock Protein 90 Inhibitor on Pain in Cancer Patients: A Systematic Review and Meta-Analysis.

    Miles, Victoria N / Patel, Roma K / Smith, Amanda G / McCall, Ryan P / Wu, Jun / Lei, Wei

    Medicina (Kaunas, Lithuania)

    2020  Volume 57, Issue 1

    Abstract: Heat shock protein 90 (Hsp90) is a molecular chaperone that plays an essential role in tumor growth. Numerous Hsp90 inhibitors have been discovered and tested in preclinical and clinical trials. Recently, several preclinical studies have demonstrated ... ...

    Abstract Heat shock protein 90 (Hsp90) is a molecular chaperone that plays an essential role in tumor growth. Numerous Hsp90 inhibitors have been discovered and tested in preclinical and clinical trials. Recently, several preclinical studies have demonstrated that Hsp90 inhibitors could modulate pain sensitization. However, no studies have evaluated the impact of Hsp90 inhibitors on pain in the patients. This study aims to summarize the pain events reported in clinical trials assessing Hsp90 inhibitors and to determine the effect of Hsp90 inhibitors on pain in patients. We searched PubMed, EBSCOhost, and clinicaltrials.gov for Hsp90 inhibitor clinical trials. The pain-related adverse events were summarized. Meta-analysis was performed using the data reported in randomized controlled trials. We identified 90 clinical trials that reported pain as an adverse effect, including 5 randomized controlled trials. The most common types of pain reported in all trials included headache, abdominal pain, and back pain. The meta-analysis showed that Hsp90 inhibitors increased the risk of abdominal pain significantly and appeared to increase the risk for back pain. In conclusion, Hsp90 inhibitor treatment could potentially increase the risk of pain. However, the meta-analysis demonstrated only moderate evidence for the connection between Hsp90 inhibitor and pain.
    MeSH term(s) Antineoplastic Agents/therapeutic use ; Cancer Pain/drug therapy ; HSP90 Heat-Shock Proteins/therapeutic use ; Humans ; Neoplasms/complications ; Neoplasms/drug therapy
    Chemical Substances Antineoplastic Agents ; HSP90 Heat-Shock Proteins
    Language English
    Publishing date 2020-12-23
    Publishing country Switzerland
    Document type Journal Article ; Meta-Analysis ; Review ; Systematic Review
    ZDB-ID 2188113-3
    ISSN 1648-9144 ; 1010-660X
    ISSN (online) 1648-9144
    ISSN 1010-660X
    DOI 10.3390/medicina57010005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A YOLO based software for automated detection and analysis of rodent behaviour in the open field arena.

    Hatton-Jones, Kyle M / Christie, Corey / Griffith, Tia A / Smith, Amanda G / Naghipour, Saba / Robertson, Kai / Russell, Jake S / Peart, Jason N / Headrick, John P / Cox, Amanda J / du Toit, Eugene F

    Computers in biology and medicine

    2021  Volume 134, Page(s) 104474

    Abstract: Rodent models are important in mechanistic studies of the physiological and pathophysiological determinants of behaviour. The Open Field Test (OFT) is one of the most commonly utilised tests to assess rodent behaviour in a novel open environment. The key ...

    Abstract Rodent models are important in mechanistic studies of the physiological and pathophysiological determinants of behaviour. The Open Field Test (OFT) is one of the most commonly utilised tests to assess rodent behaviour in a novel open environment. The key variables assessed in an OFT are general locomotor activity and exploratory behaviours and can be assessed manually or by automated systems. Although several automated systems exist, they are often expensive, difficult to use, or limited in the type of video that can be analysed. Here we describe a machine-learning algorithm - dubbed Cosevare - that uses a trained YOLOv3 DNN to identify and track movement of mice in the open-field arena. We validated Cosevare's capacity to accurately track locomotive and exploratory behaviour in 10 videos, comparing outputs generated by Cosevare with analysis by 5 manual scorers. Behavioural differences between control mice and those with diet-induced obesity (DIO) were also documented. We found the YOLOv3 based tracker to be accurate at identifying and tracking the mice within the open-field arena and in instances with variable backgrounds. Additionally, kinematic and spatial-based analysis demonstrated highly consistent scoring of locomotion, centre square duration (CSD) and entries (CSE) between Cosevare and manual scorers. Automated analysis was also able to distinguish behavioural differences between healthy control and DIO mice. The study found that a YOLOv3 based tracker is able to easily track mouse behaviour in the open field arena and supports machine learning as a potential future alternative for the assessment of animal behaviour in a wide range of species in differing environments and behavioural tests.
    MeSH term(s) Animals ; Behavior, Animal ; Exploratory Behavior ; Locomotion ; Mice ; Rodentia ; Software
    Language English
    Publishing date 2021-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 127557-4
    ISSN 1879-0534 ; 0010-4825
    ISSN (online) 1879-0534
    ISSN 0010-4825
    DOI 10.1016/j.compbiomed.2021.104474
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Spatial and temporal silencing of the human maternal UBE3A gene.

    Daily, Jennifer / Smith, Amanda G / Weeber, Edwin J

    European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society

    2012  Volume 16, Issue 6, Page(s) 587–591

    Abstract: Angelman syndrome (AS) is characterized by severe cognitive disruption, seizures, difficulty speaking and ataxia. Nearly all cases are attributed to the disruption or absence of the imprinted maternal copy of UBE3A, transcribing an E3-type ubiquitin ... ...

    Abstract Angelman syndrome (AS) is characterized by severe cognitive disruption, seizures, difficulty speaking and ataxia. Nearly all cases are attributed to the disruption or absence of the imprinted maternal copy of UBE3A, transcribing an E3-type ubiquitin ligase. Much of what is known about the molecular and biochemical changes in the CNS associated with AS has been obtained through this murine model. This widely used mouse model created by a null mutation of the maternal UBE3A gene recapitulates the major phenotypes characteristic of AS patients. The imprinting of maternal UBE3A was originally believed to be brain region specific; however recent reports using the AS mouse model have revealed a more wide-spread absence of the protein. The present study is the first to determine that the Ube3a protein ablation seen in the AS mouse model is also characteristic of AS patients and the silencing of the paternal UBE3A allele appears to be lifelong.
    MeSH term(s) Adult ; Angelman Syndrome/genetics ; Animals ; Blotting, Western ; Brain Chemistry/genetics ; Child ; Disease Models, Animal ; Female ; Gene Silencing ; Genotype ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Phenotype ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/physiology
    Chemical Substances UBE3A protein, human (EC 2.3.2.26) ; Ube3a protein, mouse (EC 2.3.2.26) ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
    Language English
    Publishing date 2012-05-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 1397146-3
    ISSN 1532-2130 ; 1090-3798
    ISSN (online) 1532-2130
    ISSN 1090-3798
    DOI 10.1016/j.ejpn.2012.03.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients.

    Grewal, Rinko / Haghighi, Mona / Huang, Shuai / Smith, Amanda G / Cao, Chuanhai / Lin, Xiaoyang / Lee, Daniel C / Teten, Nancy / Hill, Angela M / Selenica, Maj-Linda B

    Alzheimer's research & therapy

    2016  Volume 8, Issue 1, Page(s) 43

    Abstract: Background: There is a need to investigate biomarkers that are indicative of the progression of dementia in ethnic patient populations. The disparity of information in these populations has been the focus of many clinical and academic centers, including ...

    Abstract Background: There is a need to investigate biomarkers that are indicative of the progression of dementia in ethnic patient populations. The disparity of information in these populations has been the focus of many clinical and academic centers, including ours, to contribute to a higher success rate in clinical trials. In this study, we have investigated plasma biomarkers in amnestic mild cognitively impaired (aMCI) female patient cohorts in the context of ethnicity and cognitive status.
    Method: A panel of 12 biomarkers involved in the progression of brain pathology, inflammation, and cardiovascular disorders were investigated in female cohorts of African American, Hispanic, and White aMCI patients. Both biochemical and algorithmic analyses were applied to correlate biomarker levels measured during the early stages of the disease for each ethnicity.
    Results: We report elevated plasma Aβ
    Conclusions: Overall, our data displayed novel differences in the plasma biomarkers of the aMCI female cohorts where the plasma levels of several biomarkers distinguished between each ethnicity at an early aMCI stage. Identification of these plasma biomarkers encourages new areas of investigation among aMCI ethnic populations, including larger patient cohorts and longitudinal study designs.
    MeSH term(s) African Americans ; Aged ; Aged, 80 and over ; Algorithms ; Amyloid beta-Peptides/blood ; Biomarkers/blood ; Chitinase-3-Like Protein 1/blood ; Cohort Studies ; Cystatin C/blood ; Decision Trees ; Dementia/blood ; Dementia/epidemiology ; Dementia/ethnology ; Enzyme-Linked Immunosorbent Assay ; European Continental Ancestry Group ; Female ; Hispanic Americans ; Humans ; Intercellular Signaling Peptides and Proteins/blood ; Mental Status Schedule ; Middle Aged ; Peptide Fragments/blood
    Chemical Substances Amyloid beta-Peptides ; Biomarkers ; CHI3L1 protein, human ; Chitinase-3-Like Protein 1 ; Cystatin C ; GRN protein, human ; Intercellular Signaling Peptides and Proteins ; Peptide Fragments ; amyloid beta-protein (1-40) ; amyloid beta-protein (1-42)
    Language English
    Publishing date 2016--18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2506521-X
    ISSN 1758-9193 ; 1758-9193
    ISSN (online) 1758-9193
    ISSN 1758-9193
    DOI 10.1186/s13195-016-0211-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii

    Ordinario, David D / Phan, Long / Walkup IV, Ward G / Van Dyke, Yegor / Leung, Erica M / Nguyen, Michael / Smith, Amanda G / Kerr, Justin / Naeim, Mahan / Kymissis, Ioannis / Gorodetsky, Alon A

    RSC advances. 2016 June 15, v. 6, no. 62

    2016  

    Abstract: Cephalopods have recently emerged as a source of inspiration for the development of novel functional materials. Within this context, a number of studies have explored structural proteins known as reflectins, which play a key role in cephalopod adaptive ... ...

    Abstract Cephalopods have recently emerged as a source of inspiration for the development of novel functional materials. Within this context, a number of studies have explored structural proteins known as reflectins, which play a key role in cephalopod adaptive coloration in vivo and exhibit interesting properties in vitro. Herein, we report an improved high-yield strategy for the preparation and isolation of reflectins in quantities sufficient for materials applications. We first select the Doryteuthis (Loligo) pealeii reflectin A2 (RfA2) isoform as a “model” system and validate our approach for the expression and purification of this protein. We in turn fabricate RfA2-based two-terminal devices and employ both direct and alternating current measurements to demonstrate that RfA2 films conduct protons. Our findings underscore the potential of reflectins as functional materials and may allow a wider range of researchers to investigate their properties.
    Keywords Loligo ; color ; protons ; structural proteins
    Language English
    Dates of publication 2016-0615
    Size p. 57103-57107.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/c6ra05405f
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: An open-label pilot trial of minocycline in children as a treatment for Angelman syndrome.

    Grieco, Joseph C / Ciarlone, Stephanie L / Gieron-Korthals, Maria / Schoenberg, Mike R / Smith, Amanda G / Philpot, Rex M / Heussler, Helen S / Banko, Jessica L / Weeber, Edwin J

    BMC neurology

    2014  Volume 14, Page(s) 232

    Abstract: Background: Minocycline, a member of the tetracycline family, has a low risk of adverse effects and an ability to improve behavioral performance in humans with cognitive disruption. We performed a single-arm open-label trial in which 25 children ... ...

    Abstract Background: Minocycline, a member of the tetracycline family, has a low risk of adverse effects and an ability to improve behavioral performance in humans with cognitive disruption. We performed a single-arm open-label trial in which 25 children diagnosed with Angelman syndrome (AS) were administered minocycline to assess the safety and tolerability of minocycline in this patient population and determine the drug's effect on the cognitive and behavioral manifestations of the disorder.
    Methods: Participants, age 4-12 years old, were randomly selected from a pool of previously screened children for participation in this study. Each child received 3 milligrams of minocycline per kilogram of body weight per day for 8 weeks. Participants were assessed during 3 study visits: baseline, after 8-weeks of minocycline treatment and after an 8-week wash out period. The primary outcome measure was the Bayley Scales of Infant and Toddler Development 3rd Edition (BSID-III). Secondary outcome measures included the Clinical Global Impressions Scale (CGI), Vineland Adaptive Behavior Scales 2nd Edition (VABS-II), Preschool Language Scale 4th Edition (PLS-IV) and EEG scores. Observations were considered statistically significant if p < 0.05 using ANOVA and partial eta squared (η(2)) was calculated to show effect size. Multiple comparisons testing between time points were carried out using Dunnett's post hoc testing.
    Results: Significant improvement in the mean raw scores of the BSID-III subdomains communication and fine motor ability as well as the subdomains auditory comprehension and total language ability of the PLS-IV when baseline scores were compared to scores after the washout period. Further, improvements were observed in the receptive communication subdomain of the VABS-II after treatment with minocycline. Finally, mean scores of the BSID-III self-direction subdomain and CGI scale score were significantly improved both after minocycline treatment and after the wash out period.
    Conclusion: The clinical and neuropsychological measures suggest minocycline was well tolerated and causes improvements in the adaptive behaviors of this sample of children with Angelman syndrome. While the optimal dosage and the effects of long-term use still need to be determined, these findings suggest further investigation into the effect minocycline has on patients with Angelman syndrome is warranted.
    Trial registration: NCT01531582 - clinicaltrials.gov.
    MeSH term(s) Angelman Syndrome/complications ; Angelman Syndrome/drug therapy ; Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/adverse effects ; Anti-Bacterial Agents/pharmacology ; Child ; Child, Preschool ; Cognition Disorders/drug therapy ; Cognition Disorders/etiology ; Female ; Humans ; Male ; Minocycline/administration & dosage ; Minocycline/adverse effects ; Minocycline/pharmacology ; Pilot Projects ; Treatment Outcome
    Chemical Substances Anti-Bacterial Agents ; Minocycline (FYY3R43WGO)
    Language English
    Publishing date 2014-12-10
    Publishing country England
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041347-6
    ISSN 1471-2377 ; 1471-2377
    ISSN (online) 1471-2377
    ISSN 1471-2377
    DOI 10.1186/s12883-014-0232-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Topiramate Overdose: A Case Report and Literature Review.

    Smith, Amanda G. / Brauer, Heidi R. / Catalano, Glenn / Catalano, Maria C.

    Epilepsy & behavior : E&B

    2001  Volume 2, Issue 6, Page(s) 603–607

    Abstract: Topiramate is a medication introduced in the United States in 1997 for the treatment of epilepsy. Studies are currently underway to determine its effectiveness in the treatment of multiple conditions including bipolar disorder. It is generally well ... ...

    Abstract Topiramate is a medication introduced in the United States in 1997 for the treatment of epilepsy. Studies are currently underway to determine its effectiveness in the treatment of multiple conditions including bipolar disorder. It is generally well tolerated at doses commonly used in the clinical setting, however, there is little information regarding its safety in overdose. We report the case of a 24-year-old woman who ingested 4000 mg of topiramate in a suicide attempt. She was asymptomatic following the overdose and did not develop any adverse sequelae. In this article we will discuss the commonly seen side effects of topiramate use and examine the available data concerning topiramate overdose. We will review recommendations for the management of such an overdose.
    Language English
    Publishing date 2001-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2010587-3
    ISSN 1525-5069 ; 1525-5050
    ISSN (online) 1525-5069
    ISSN 1525-5050
    DOI 10.1006/ebeh.2001.0299
    Database MEDical Literature Analysis and Retrieval System OnLINE

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