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  1. Article: Assay Techniques and Test Development for COVID-19 Diagnosis.

    Carter, Linda J / Garner, Linda V / Smoot, Jeffrey W / Li, Yingzhu / Zhou, Qiongqiong / Saveson, Catherine J / Sasso, Janet M / Gregg, Anne C / Soares, Divya J / Beskid, Tiffany R / Jervey, Susan R / Liu, Cynthia

    ACS central science

    2020  Volume 6, Issue 5, Page(s) 591–605

    Keywords covid19
    Language English
    Publishing date 2020-04-30
    Publishing country United States
    Document type News
    ISSN 2374-7943
    ISSN 2374-7943
    DOI 10.1021/acscentsci.0c00501
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Assay Techniques and Test Development for COVID-19 Diagnosis

    Carter, Linda J. / Garner, Linda V. / Smoot, Jeffrey W. / Li, Yingzhu / Zhou, Qiongqiong / Saveson, Catherine J. / Sasso, Janet M. / Gregg, Anne C. / Soares, Divya J. / Beskid, Tiffany R. / Jervey, Susan R. / Liu, Cynthia

    ACS Cent. Sci.

    Abstract: An ongoing theme of the COVID-19 pandemic is the need for widespread availability of accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral antibodies in infected individuals. This report describes various assay techniques and ... ...

    Abstract An ongoing theme of the COVID-19 pandemic is the need for widespread availability of accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral antibodies in infected individuals. This report describes various assay techniques and tests for COVID-19 diagnosis. Most tests for early detection of SARS-CoV-2 RNA rely on the reverse transcription-polymerase chain reaction, but isothermal nucleic acid amplification assays, including transcription-mediated amplification and CRISPR-based methodologies, are promising alternatives. Identification of individuals who have developed antibodies to the SARS-CoV-2 virus requires serological tests, including enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay. This report also provides an overview of current development in COVID-19 diagnostic techniques and products to facilitate future improvement and innovation.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #142071
    Database COVID19

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  3. Article ; Online: Assay Techniques and Test Development for COVID-19 Diagnosis

    Carter, Linda J. / Garner, Linda V. / Smoot, Jeffrey W. / Li, Yingzhu / Zhou, Qiongqiong / Saveson, Catherine J. / Sasso, Janet M. / Gregg, Anne C. / Soares, Divya J. / Beskid, Tiffany R. / Jervey, Susan R. / Liu, Cynthia

    ACS Central Science

    2020  Volume 6, Issue 5, Page(s) 591–605

    Keywords covid19
    Language English
    Publisher American Chemical Society (ACS)
    Publishing country us
    Document type Article ; Online
    ISSN 2374-7943
    DOI 10.1021/acscentsci.0c00501
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  4. Article: Conserved eukaryotic histone-fold residues substituted into an archaeal histone increase DNA affinity but reduce complex flexibility.

    Soares, Divya J / Marc, Frédéric / Reeve, John N

    Journal of bacteriology

    2003  Volume 185, Issue 11, Page(s) 3453–3457

    Abstract: Although the archaeal and eukaryotic nucleosome core histones evolved from a common ancestor, conserved lysine residues are present at DNA-binding locations in all four eukaryotic histones that are not present in the archaeal histones. Introduction of ... ...

    Abstract Although the archaeal and eukaryotic nucleosome core histones evolved from a common ancestor, conserved lysine residues are present at DNA-binding locations in all four eukaryotic histones that are not present in the archaeal histones. Introduction of lysine residues at the corresponding locations into an archaeal histone, HMfB, generated a variant with increased affinity for DNA that formed more compact complexes with DNA. However, these complexes no longer facilitated the circularization of short DNA molecules and had lost the flexibility to wrap DNA alternatively in either a negative or positive supercoil.
    MeSH term(s) Amino Acid Sequence ; Archaeal Proteins/chemistry ; Archaeal Proteins/genetics ; Archaeal Proteins/metabolism ; Conserved Sequence ; DNA, Circular/metabolism ; Eukaryotic Cells ; Histones/chemistry ; Histones/genetics ; Histones/metabolism ; Models, Molecular ; Molecular Sequence Data ; Nucleosomes/chemistry ; Protein Folding
    Chemical Substances Archaeal Proteins ; DNA, Circular ; Histones ; Nucleosomes
    Language English
    Publishing date 2003-06
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.185.11.3453-3457.2003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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