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Article: Optical Genome Mapping in Routine Cytogenetic Diagnosis of Acute Leukemia.

Soler, Gwendoline / Ouedraogo, Zangbéwendé Guy / Goumy, Carole / Lebecque, Benjamin / Aspas Requena, Gaspar / Ravinet, Aurélie / Kanold, Justyna / Véronèse, Lauren / Tchirkov, Andrei

Cancers

2023 Volume 15, Issue 7

Abstract: Cytogenetic aberrations are found in 65% of adults and 75% of children with acute leukemia. Specific aberrations are used as markers for the prognostic stratification of patients. The current standard cytogenetic procedure for acute leukemias is ... ...

Abstract	Cytogenetic aberrations are found in 65% of adults and 75% of children with acute leukemia. Specific aberrations are used as markers for the prognostic stratification of patients. The current standard cytogenetic procedure for acute leukemias is karyotyping in combination with FISH and RT-PCR. Optical genome mapping (OGM) is a new technology providing a precise identification of chromosomal abnormalities in a single approach. In our prospective study, the results obtained using OGM and standard techniques were compared in 29 cases of acute myeloid (AML) or lymphoblastic leukemia (ALL). OGM detected 73% (53/73) of abnormalities identified by standard methods. In AML cases, two single clones and three subclones were missed by OGM, but the assignment of patients to cytogenetic risk groups was concordant in all patients. OGM identified additional abnormalities in six cases, including one cryptic structural variant of clinical interest and two subclones. In B-ALL cases, OGM correctly detected all relevant aberrations and revealed additional potentially targetable alterations. In T-ALL cases, OGM characterized a complex karyotype in one case and identified additional abnormalities in two others. In conclusion, OGM is an attractive alternative to current multiple cytogenetic testing in acute leukemia that simplifies the procedure and reduces costs.
Language	English
Publishing date	2023-04-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15072131
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Article: Feasibility of Optical Genome Mapping from Placental and Umbilical Cord Sampled after Spontaneous or Therapeutic Pregnancy Termination.

Goumy, Carole / Ouedraogo, Zangbéwendé Guy / Bellemonte, Elodie / Eymard-Pierre, Eleonore / Soler, Gwendoline / Perthus, Isabelle / Pebrel-Richard, Céline / Gouas, Laetitia / Salaun, Gaëlle / Véronèse, Lauren / Laurichesse, Hélène / Darcha, Claude / Tchirkov, Andrei

Diagnostics (Basel, Switzerland)

2023 Volume 13, Issue 23

Abstract: Optical genome mapping (OGM) is an alternative to classical cytogenetic techniques to improve the detection rate of clinically significant genomic abnormalities. The isolation of high-molecular-weight (HMW) DNA is critical for a successful OGM analysis. ... ...

Abstract	Optical genome mapping (OGM) is an alternative to classical cytogenetic techniques to improve the detection rate of clinically significant genomic abnormalities. The isolation of high-molecular-weight (HMW) DNA is critical for a successful OGM analysis. HMW DNA quality depends on tissue type, sample size, and storage conditions. We assessed the feasibility of OGM analysis of DNA from nine umbilical cord (UC) and six chorionic villus (CV) samples collected after the spontaneous or therapeutic termination of pregnancy. We analyzed quality control metrics provided by the Saphyr system (Bionano Genomics) and assessed the length of extracted DNA molecules using pulsed-field capillary electrophoresis. OMG data were successfully analyzed for all six CV samples. Five of the UC samples did not meet the Saphyr quality criteria, mainly due to poor DNA quality. In this regard, we found that DNA quality assessment with pulsed-field capillary electrophoresis can predict a successful OGM analysis. OGM data were fully concordant with the results of standard cytogenetic methods. Moreover, OGM detected an average of 14 additional structural variants involving OMIM genes per sample. On the basis of our results, we established the optimal conditions for sample storage and preparation required for a successful OGM analysis. We recommend checking DNA quality before analysis with pulsed-field capillary electrophoresis if the storage conditions were not ideal or if the quality of the sample is poor. OGM can therefore be performed on fetal tissue harvested after the termination of pregnancy, which opens up the perspective for improved diagnostic yield.
Language	English
Publishing date	2023-11-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics13233576
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Article ; Online: Optical genome mapping for prenatal diagnosis: A prospective study.

Goumy, Carole / Guy Ouedraogo, Zangbéwendé / Soler, Gwendoline / Eymard-Pierre, Eleonore / Laurichesse, Hélène / Delabaere, Amélie / Gallot, Denis / Bouchet, Pamela / Perthus, Isabelle / Pebrel-Richard, Céline / Gouas, Laetitia / Salaun, Gaëlle / Salse, Jérôme / Véronèse, Lauren / Tchirkov, Andrei

Clinica chimica acta; international journal of clinical chemistry

2023 Volume 551, Page(s) 117594

Abstract: Purpose: Cytogenetic analysis provides important information for prenatal decision-making and genetic counseling. Optical genome mapping (OGM) has demonstrated its performances in retrospective studies. In our prospective study, we assessed the quality ... ...

Abstract	Purpose: Cytogenetic analysis provides important information for prenatal decision-making and genetic counseling. Optical genome mapping (OGM) has demonstrated its performances in retrospective studies. In our prospective study, we assessed the quality of DNA obtained from cultures of amniotic fluid (AF) and chorionic villi (CV) and evaluated the ability of OGM to detect all clinically relevant aberrations identified by standard methods. Methods: A total of 37 prenatal samples from pregnancies with a fetal anomaly on ultrasound were analyzed prospectively by OGM between January 1, 2021 and June 31, 2022. OGM results were interpreted blindly and compared to the results obtained by standard techniques. Results: OGM results were interpretable in 92% of samples. We observed 100% concordance between OGM and karyotype and/or chromosomal microarray results. In addition, OGM identified a median of 30 small (<100 kb) structural variations per case with the involvement of 12 OMIM genes, of which 3 were OMIM morbid genes. Conclusion: This prospective study showed OGM performed well in detecting genomic alterations in cell cultures from prenatal samples. The place of OGM in relation to CMA or exome sequencing remains to be defined in order to optimize the prenatal diagnostic procedure.
MeSH term(s)	Pregnancy ; Female ; Humans ; Prospective Studies ; Retrospective Studies ; Karyotyping ; Cytogenetic Analysis ; Chromosome Mapping ; Prenatal Diagnosis ; Chromosome Aberrations
Language	English
Publishing date	2023-10-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2023.117594
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Article ; Online: Prenatal diagnosis on chorionic villi using molecular techniques should be performed from mesenchymal core rather than from direct villi.

Toutain, Jérôme / Soler, Gwendoline / Horovitz, Jacques / Saura, Robert

Prenatal diagnosis

2011 Volume 31, Issue 11, Page(s) 1111–2; author reply 1113

MeSH term(s)	Chorionic Villi/chemistry ; Chromosomes, Human, Pair 18 ; Comparative Genomic Hybridization/methods ; DNA/analysis ; Female ; Humans ; Mosaicism/embryology ; Pregnancy ; Trisomy/diagnosis
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2011-11
Publishing country	England
Document type	Comment ; Letter
ZDB-ID	82031-3
ISSN	1097-0223 ; 0197-3851
ISSN (online)	1097-0223
ISSN	0197-3851
DOI	10.1002/pd.2776
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Article ; Online: Isolated isochromosomes i(X)(p10) and idic(X)(q13) are associated with myeloid malignancies and dysplastic features.

Penther, Dominique / Etancelin, Pascaline / Lusina, Daniel / Bidet, Audrey / Quilichini, Benoit / Gaillard, Baptiste / Rafdord-Weiss, Isabelle / Mozziconacci, Marie Joelle / Ittel, Antoine / Roche-Lestienne, Catherine / Barin, Carole / Soler, Gwendoline / Daudignon, Agnes / Nadal, Nathalie / Chapiro, Elise / Lefebvre, Christine / Godon, Catherine / Nadeau, Gwenael / Mugneret, Francine /

Richebourg, Steven / Viailly, Pierre-Julien / Ferret, Yann / Nguyen-Khac, Florence / Eclache, Virginie

American journal of hematology

2019 Volume 94, Issue 11, Page(s) E285–E288

MeSH term(s)	Age Distribution ; Aged ; Bone Marrow/pathology ; Chromosomes, Human, X/genetics ; Chromosomes, Human, X/ultrastructure ; DNA-Binding Proteins/genetics ; Female ; Follow-Up Studies ; Genes, Neoplasm ; Humans ; Isochromosomes ; Leukemia, Myeloid/epidemiology ; Leukemia, Myeloid/genetics ; Leukemia, Myeloid/pathology ; Male ; Middle Aged ; Mutation ; Myelodysplastic Syndromes/epidemiology ; Myelodysplastic Syndromes/genetics ; Myelodysplastic Syndromes/pathology ; Neoplasm Proteins/genetics ; Neoplasms, Second Primary/genetics ; Neoplasms, Second Primary/pathology ; Proto-Oncogene Proteins/genetics ; Sex Distribution
Chemical Substances	DNA-Binding Proteins ; Neoplasm Proteins ; Proto-Oncogene Proteins ; TET2 protein, human (EC 1.13.11.-)
Language	English
Publishing date	2019-08-26
Publishing country	United States
Document type	Letter
ZDB-ID	196767-8
ISSN	1096-8652 ; 0361-8609
ISSN (online)	1096-8652
ISSN	0361-8609
DOI	10.1002/ajh.25601
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Article ; Online: Proportion of parents agreeing to delay fetal karyotyping until the third trimester of pregnancy in cases with an indication.

Toutain, Jérôme / Lemaire-Coustel, Marie-Anne / Begorre, Marianne / Montaubin, Orianne / Soler, Gwendoline / Taine, Laurence / Horovitz, Jacques / Saura, Robert

Fetal diagnosis and therapy

2012 Volume 31, Issue 2, Page(s) 115–121

Abstract: Objective: To assess the extent to which couples who could benefit from fetal karyotyping during the first or second trimester would agree to delay the examination until the third trimester.: Methods: In this prospective monocentric study, the same ... ...

Abstract	Objective: To assess the extent to which couples who could benefit from fetal karyotyping during the first or second trimester would agree to delay the examination until the third trimester. Methods: In this prospective monocentric study, the same physician suggested to some couples to delay fetal karyotyping until the third trimester. Results: 458 couples participated in this study. 230 couples (230/458 = 50.2%) refused to delay the examination until the third trimester of pregnancy (group 1). For these patients, four chromosomal abnormalities led to the termination of pregnancy. Fifty-six couples (56/458 = 12.2%) who initially agreed to delay the fetal karyotyping later changed their minds (group 2). 104 couples (104/458 = 22.7%) agreed to delay the examination (group 3). For these patients, one trisomy 21 was diagnosed and led to the subsequent termination of the pregnancy at 33 weeks of amenorrhea. Sixty-eight couples (68/458 = 14.8%) refused any form of invasive prenatal diagnosis (group 4). There was no difference in the rate of preterm premature rupture of membranes, pregnancy term, premature birth rate and birth weight between the four groups. Conclusions: Our study reports that about a quarter of couples did indeed agree to delay fetal karyotype assessment until the third trimester of pregnancy.
MeSH term(s)	Abortion, Induced ; Adult ; Amniocentesis/adverse effects ; Amniocentesis/methods ; Chromosome Disorders/diagnosis ; Chromosome Disorders/genetics ; Female ; Genetic Testing/methods ; Gestational Age ; Humans ; Infant, Newborn ; Karyotyping/methods ; Male ; Parents/psychology ; Patient Compliance ; Pregnancy ; Pregnancy Trimester, Second ; Pregnancy Trimester, Third ; Prenatal Diagnosis/methods ; Prospective Studies ; Treatment Refusal
Language	English
Publishing date	2012
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1066460-9
ISSN	1421-9964 ; 1015-3837
ISSN (online)	1421-9964
ISSN	1015-3837
DOI	10.1159/000334067
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Article ; Online: NUP98-MLL fusion in human acute myeloblastic leukemia.

Kaltenbach, Sophie / Soler, Gwendoline / Barin, Carole / Gervais, Carine / Bernard, Olivier A / Penard-Lacronique, Virginie / Romana, Serge P

Blood

2010 Volume 116, Issue 13, Page(s) 2332–2335

Abstract: Posttranscriptional modifications of histones play important roles in the control of chromatin structure and transcription. H3K4 (histone H3 lysine 4) methylation by the SET domain of the trithorax-group protein MLL (mixed-lineage leukemia) is important ... ...

Abstract	Posttranscriptional modifications of histones play important roles in the control of chromatin structure and transcription. H3K4 (histone H3 lysine 4) methylation by the SET domain of the trithorax-group protein MLL (mixed-lineage leukemia) is important for the control of homeobox (HOX) gene expression during development. MLL is tethered to the HOXA locus through interaction of its amino-terminus with menin. MLL fusion proteins associated with human leukemia contain the menin interaction peptide and frequently recruit H3K79 (histone H3 lysine 79) methylation activity. This allows sustained expression of HOXA genes important for cellular transformation. We have characterized a novel recurrent chromosomal aberration, inv(11)(p15q23), as an isolated chromosomal abnormality in 2 patients with acute myeloid leukemia. This aberration is predicted to result in the expression of an NUP98 (nucleoporin 98 kDa)-MLL fusion protein that is unable to interact with menin. As expected, low levels of HOXA gene expression were observed in the patients' samples. This fusion protein is predicted to participate in cellular transformation by activating MLL targets other than HOXA genes.
MeSH term(s)	Adult ; Aged ; Base Sequence ; Cell Transformation, Neoplastic/genetics ; Chromosome Inversion ; Chromosomes, Human, Pair 11/genetics ; DNA Primers/genetics ; DNA, Neoplasm/genetics ; Female ; Gene Expression ; Genes, Homeobox ; Histone-Lysine N-Methyltransferase ; Histones/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/metabolism ; Male ; Myeloid-Lymphoid Leukemia Protein/genetics ; Myeloid-Lymphoid Leukemia Protein/metabolism ; Nuclear Pore Complex Proteins/genetics ; Nuclear Pore Complex Proteins/metabolism ; Oncogene Fusion ; Oncogene Proteins, Fusion/genetics ; Oncogene Proteins, Fusion/metabolism ; Proto-Oncogene Proteins/metabolism
Chemical Substances	DNA Primers ; DNA, Neoplasm ; Histones ; Homeodomain Proteins ; KMT2A protein, human ; MEN1 protein, human ; Nuclear Pore Complex Proteins ; Nup98 protein, human ; Oncogene Proteins, Fusion ; Proto-Oncogene Proteins ; Myeloid-Lymphoid Leukemia Protein (149025-06-9) ; HoxA protein (157907-48-7) ; Histone-Lysine N-Methyltransferase (EC 2.1.1.43)
Language	English
Publishing date	2010-06-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood-2010-04-277806
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Article: Proportion of Parents Agreeing to Delay Fetal Karyotyping until the Third Trimester of Pregnancy in Cases with an Indication

Toutain, Jérôme / Lemaire-Coustel, Marie-Anne / Begorre, Marianne / Montaubin, Orianne / Soler, Gwendoline / Taine, Laurence / Horovitz, Jacques / Saura, Robert

Fetal Diagnosis and Therapy

2012 Volume 31, Issue 2, Page(s) 115–121

Abstract: Objective: To assess the extent to which couples who could benefit from fetal karyotyping during the first or second trimester would agree to delay the examination until the third trimester. Methods: In this prospective monocentric study, the same ... ...

Institution	Laboratoire de cytogénétique, service de génétique médicale, maternité Pellegrin, CHU de Bordeaux Université Bordeaux Segalen, et Service de gynécologie-obstétrique, maternité Pellegrin, CHU de Bordeaux, Bordeaux, France
Abstract	Objective: To assess the extent to which couples who could benefit from fetal karyotyping during the first or second trimester would agree to delay the examination until the third trimester. Methods: In this prospective monocentric study, the same physician suggested to some couples to delay fetal karyotyping until the third trimester. Results: 458 couples participated in this study. 230 couples (230/458 = 50.2%) refused to delay the examination until the third trimester of pregnancy (group 1). For these patients, four chromosomal abnormalities led to the termination of pregnancy. Fifty-six couples (56/458 = 12.2%) who initially agreed to delay the fetal karyotyping later changed their minds (group 2). 104 couples (104/458 = 22.7%) agreed to delay the examination (group 3). For these patients, one trisomy 21 was diagnosed and led to the subsequent termination of the pregnancy at 33 weeks of amenorrhea. Sixty-eight couples (68/458 = 14.8%) refused any form of invasive prenatal diagnosis (group 4). There was no difference in the rate of preterm premature rupture of membranes, pregnancy term, premature birth rate and birth weight between the four groups. Conclusions: Our study reports that about a quarter of couples did indeed agree to delay fetal karyotype assessment until the third trimester of pregnancy.
Keywords	Fetal karyotype ; Amniocentesis ; Third trimester of pregnancy
Language	English
Publishing date	2012-01-31
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Original Paper
ZDB-ID	1066460-9
ISSN	1421-9964 ; 1015-3837
ISSN (online)	1421-9964
ISSN	1015-3837
DOI	10.1159/000334067
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Article ; Online: MLL insertion with MLL-MLLT3 gene fusion in acute leukemia: case report and review of the literature.

Soler, Gwendoline / Radford, Isabelle / Meyer, Claus / Marschalek, Rolf / Brouzes, Chantal / Ghez, David / Romana, Serge / Berger, Roland

Cancer genetics and cytogenetics

2008 Volume 183, Issue 1, Page(s) 53–59

Abstract: A new chromosomal insertion involving the MLL gene was detected by fluorescence in situ hybridization in a patient with acute myeloblastic leukemia (AML) and a t(9;11)(p21;q13). Genomic polymerase chain reaction confirmed the MLL-MLLT3 gene fusion. A ... ...

Abstract	A new chromosomal insertion involving the MLL gene was detected by fluorescence in situ hybridization in a patient with acute myeloblastic leukemia (AML) and a t(9;11)(p21;q13). Genomic polymerase chain reaction confirmed the MLL-MLLT3 gene fusion. A review of the literature on MLL insertions shows that the opposite orientation of the genes involved in the fusion plays a role in the genesis of the rearrangement in most of the cases reported.
MeSH term(s)	Adult ; Amino Acid Sequence ; Base Sequence ; Chromosome Breakage ; Cytogenetic Analysis ; Female ; Histone-Lysine N-Methyltransferase ; Humans ; Leukemia, Myeloid, Acute/genetics ; Molecular Sequence Data ; Mutagenesis, Insertional ; Myeloid-Lymphoid Leukemia Protein/genetics ; Nuclear Proteins/genetics ; Oncogene Proteins, Fusion/genetics
Chemical Substances	KMT2A protein, human ; MLL-MLLT3 fusion protein, human ; MLLT3 protein, human ; Nuclear Proteins ; Oncogene Proteins, Fusion ; Myeloid-Lymphoid Leukemia Protein (149025-06-9) ; Histone-Lysine N-Methyltransferase (EC 2.1.1.43)
Language	English
Publishing date	2008-05-09
Publishing country	United States
Document type	Case Reports ; Journal Article ; Review
ZDB-ID	800806-1
ISSN	1873-4456 ; 0165-4608
ISSN (online)	1873-4456
ISSN	0165-4608
DOI	10.1016/j.cancergencyto.2008.01.016
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Article ; Online: Parallel FISH and immunohistochemical studies of ALK status in 3244 non-small-cell lung cancers reveal major discordances.

Cabillic, Florian / Gros, Audrey / Dugay, Frédéric / Begueret, Hugues / Mesturoux, Laura / Chiforeanu, Dan Cristian / Dufrenot, Leila / Jauffret, Vincent / Dachary, Dominique / Corre, Romain / Lespagnol, Alexandra / Soler, Gwendoline / Dagher, Julien / Catros, Véronique / Le Calve, Michèle / Merlio, Jean-Philippe / Belaud-Rotureau, Marc-Antoine

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

2014 Volume 9, Issue 3, Page(s) 295–306

Abstract: Introduction: Anaplastic lymphoma kinase (ALK) rearrangements occur in 1% to 7% of non-small-cell lung cancers (NSCLCs). Crizotinib, an ALK inhibitor, has been demonstrated to provide dramatic clinical benefits in ALK-positive advanced-stage NSCLC. ... ...

Abstract	Introduction: Anaplastic lymphoma kinase (ALK) rearrangements occur in 1% to 7% of non-small-cell lung cancers (NSCLCs). Crizotinib, an ALK inhibitor, has been demonstrated to provide dramatic clinical benefits in ALK-positive advanced-stage NSCLC. Fluorescent in situ hybridization (FISH) has been established in clinical trials as the standard procedure method for detecting ALK rearrangements. Although the detection of ALK by immunohistochemistry (IHC) has been proposed for the screening of patients, large-scale studies are warranted to validate such a hierarchical approach. Methods: In this article, we report the largest series thus far of parallel FISH and IHC ALK testing in 3244 consecutive NSCLC cases analyzed at two independent French centers. Results: FISH-positive and/or IHC-positive results were demonstrated in 150 of 3244 cases (4.6%). An imbalanced sex ratio was detected, with women exhibiting a 2.2-fold relative risk for an alteration. Strikingly, only 80 of 150 specimens were classified as ALK positive by both techniques. The specimens with discordant FISH/IHC analyses were FISH-positive/IHC-negative (36), FISH-negative/IHC-positive (19), or FISH-noncontributive/IHC-positive (15). Thus, a single FISH or IHC analysis performed alone would have failed to detect approximately one-fourth of the ALK-positive cases with similar findings in our two centers. Conclusions: This study highlights the feasibility of systematic NSCLC testing by both FISH and IHC in routine practice. Many preanalytical factors may account for the apparent discrepancies between both methods, suggesting that hierarchical screening may underscore ALK-positive cases. This significant level of discrepancy supports the need of combined testing to optimize the detection of ALK-inhibitor-eligible patients given that some patients with discordant testing were found to respond to crizotinib.
MeSH term(s)	Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Adenocarcinoma/pathology ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Carcinoma, Adenosquamous/genetics ; Carcinoma, Adenosquamous/metabolism ; Carcinoma, Adenosquamous/pathology ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/pathology ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology ; Cohort Studies ; Female ; Follow-Up Studies ; Gene Rearrangement ; Humans ; Immunoenzyme Techniques/methods ; In Situ Hybridization, Fluorescence/methods ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Receptor Protein-Tyrosine Kinases/genetics ; Receptor Protein-Tyrosine Kinases/metabolism ; Young Adult
Chemical Substances	Biomarkers, Tumor ; Receptor Protein-Tyrosine Kinases (EC 2.7.10.1) ; anaplastic lymphoma kinase (EC 2.7.10.1)
Language	English
Publishing date	2014-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2432037-7
ISSN	1556-1380 ; 1556-0864
ISSN (online)	1556-1380
ISSN	1556-0864
DOI	10.1097/JTO.0000000000000072
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