LIVIVO - Search results -

Search results

Result 1 - 10 of total 12

Search options

Article ; Online: Vitamin B3 Containing Polymers for Nanodelivery.

Mapfumo, Prosper P / Solomun, Jana I / Becker, Friedrich / Moek, Elisabeth / Leiske, Meike N / Rudolph, Lenhard K / Brendel, Johannes C / Traeger, Anja

Macromolecular bioscience

2024 , Page(s) e2400002

Abstract: Polymeric nanoparticles (NPs) with an integrated dual delivery system enable the controlled release of bioactive molecules and drugs, providing therapeutic advantages. Key design targets include high biocompatibility, cellular uptake, and encapsulating ... ...

Abstract	Polymeric nanoparticles (NPs) with an integrated dual delivery system enable the controlled release of bioactive molecules and drugs, providing therapeutic advantages. Key design targets include high biocompatibility, cellular uptake, and encapsulating efficiency. In this study, a polymer library derived from niacin, also known as vitamin B3 is synthesized. The library comprises poly(2-(acryloyloxy)ethyl nicotinate) (PAEN), poly(2-acrylamidoethyl nicotinate) (PAAEN), and poly(N-(2-acrylamidoethyl)nicotinamide) (PAAENA), with varying hydrophilicity in the backbone and pendant group linker. All polymers are formulated, and those with increased hydrophobicity yield NPs with homogeneous spherical distribution and diameters below 150 nm, as confirmed by scanning electron microscopy and dynamic light scattering. Encapsulation studies utilizing a model drug, neutral lipid orange (NLO), reveal the influence of polymer backbone on encapsulation efficiency. Specifically, efficiencies of 46% and 96% are observed with acrylate and acrylamide backbones, respectively. Biological investigations showed that P(AEN) and P(AAEN) NPs are non-toxic up to 300 µg mL
Language	English
Publishing date	2024-03-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2039130-4
ISSN	1616-5195 ; 1616-5187
ISSN (online)	1616-5195
ISSN	1616-5187
DOI	10.1002/mabi.202400002
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- Full text online
- Order with fees

Article ; Online: Nanoparticle sizing in the field of nanomedicine: Power of an analytical ultracentrifuge.

Cinar, Gizem / Solomun, Jana I / Mapfumo, Prosper / Traeger, Anja / Nischang, Ivo

Analytica chimica acta

2022 Volume 1205, Page(s) 339741

Abstract: Hydrodynamic and light scattering methods are urgently required for accurate characterization of nanoparticles (NPs) in the field of nanomedicine to unveil their sizes and distributions. A fundamental characterization approach in the field of ... ...

Abstract	Hydrodynamic and light scattering methods are urgently required for accurate characterization of nanoparticles (NPs) in the field of nanomedicine to unveil their sizes and distributions. A fundamental characterization approach in the field of nanomedicines is, next to standard batch dynamic light scattering (DLS) and increasingly more applied (asymmetrical flow) field-flow fractionation (FFF) coupled to multi-angle laser light scattering (MALLS), the utilization of an analytical ultracentrifuge (AUC). Here, we demonstrate the power of an AUC in comparison to batch DLS and FFF-MALLS to decipher, in detail, the size and dispersity of pharma-relevant, commercial and in-house prepared soft matter NPs, suitable for life science applications. In this study, size and dispersity of poly(lactic-co-glycolic acid) (PLGA) NPs and in-house prepared NPs, consisting of the commercially available pharmapolymer Eudragit® E or of a polymer of similar composition synthesized via reversible addition fragmentation chain transfer (RAFT) polymerization, were investigated. Simultaneously, an insight on the presence of the utilized surfactant on the NP formulations, which is usually limited with other techniques, could be achieved by multi-speed experiments with the AUC in one experimental setting. While the repeatability and ruggedness of observations with modern AUC instruments of the newest generation is demonstrated, the results are further underpinned by the classical relations of hydrodynamics. Investigations aiming at hydrodynamic diameters (from DLS) and radii of gyration (from FFF-MALLS) are critically discussed and compared to the repeatable and rugged investigations by an AUC. The latter is proven to provide a self-sufficient experimental approach for NP characterization in the field of nanomedicine based on absolute principles, compares well to FFF-MALLS, and can unravel issues in NP sizing that arise when more common techniques, such as DLS, are used.
MeSH term(s)	Dynamic Light Scattering ; Fractionation, Field Flow/methods ; Nanomedicine ; Nanoparticles ; Particle Size
Language	English
Publishing date	2022-03-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1483436-4
ISSN	1873-4324 ; 0003-2670
ISSN (online)	1873-4324
ISSN	0003-2670
DOI	10.1016/j.aca.2022.339741
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Efficient Transfection via an Unexpected Mechanism by Near Neutral Polypiperazines with Tailored Response to Endosomal pH.

Hausig-Punke, Franziska / Dekevic, Gregor / Sobotta, Fabian H / Solomun, Jana I / Richter, Friederike / Salzig, Denise / Traeger, Anja / Brendel, Johannes C

Macromolecular bioscience

2023 Volume 23, Issue 5, Page(s) e2200517

Abstract: Cationic pH-responsive polymers promise to overcome critical challenges in cellular delivery. Ideally, the polymers become selectively charged along the endosomal pathway disturbing only the local membrane and avoiding unintended interactions or ... ...

Abstract	Cationic pH-responsive polymers promise to overcome critical challenges in cellular delivery. Ideally, the polymers become selectively charged along the endosomal pathway disturbing only the local membrane and avoiding unintended interactions or cytotoxic side effects at physiological conditions. Polypiperazines represent a novel, hydrophilic class of pH-responsive polymers whose response can be tuned within the relevant pH range (5-7.4). The authors discovered that the polypiperazines are effectively binding plasmid DNA (pDNA) and demonstrate high efficiency in transfection. By design of experiments (DoE), a wide parameter space (pDNA and polymer concentration) is screened to identify the range of effective concentrations for transfection. An isopropyl modified polypiperazine is highly efficient over a wide range of concentrations outperforming linear polyethylenimine (l-PEI, 25 kDa) in regions of low N*/P ratios. A quantitative polymerase chain reaction (qPCR) surprisingly revealed that the pDNA within the piperazine-based polyplexes can be amplified in contrast to polyplexes based on l-PEI. The pDNA must therefore be more accessible and bound differently than for other known transfection polymers. Considering the various opportunities to further optimize their structure, polypiperazines represent a promising platform for designing effective soluble polymeric vectors, which are charge-neutral at physiological conditions.
MeSH term(s)	Transfection ; Plasmids/genetics ; DNA/genetics ; DNA/metabolism ; Polymers/chemistry ; Hydrogen-Ion Concentration ; Polyethyleneimine/chemistry
Chemical Substances	DNA (9007-49-2) ; Polymers ; Polyethyleneimine (9002-98-6)
Language	English
Publishing date	2023-01-31
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2039130-4
ISSN	1616-5195 ; 1616-5187
ISSN (online)	1616-5195
ISSN	1616-5187
DOI	10.1002/mabi.202200517
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Core-crosslinked, temperature- and pH-responsive micelles: design, physicochemical characterization, and gene delivery application.

Leer, Katharina / Cinar, Gizem / Solomun, Jana I / Martin, Liam / Nischang, Ivo / Traeger, Anja

Nanoscale

2021 Volume 13, Issue 46, Page(s) 19412–19429

Abstract: Stimuli-responsive block copolymer micelles can provide tailored properties for the efficient delivery of genetic material. In particular, temperature- and pH-responsive materials are of interest, since their physicochemical properties can be easily ... ...

Abstract	Stimuli-responsive block copolymer micelles can provide tailored properties for the efficient delivery of genetic material. In particular, temperature- and pH-responsive materials are of interest, since their physicochemical properties can be easily tailored to meet the requirements for successful gene delivery. Within this study, a stimuli-responsive micelle system for gene delivery was designed based on a diblock copolymer consisting of poly(
MeSH term(s)	Gene Transfer Techniques ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; Micelles ; Temperature
Chemical Substances	Micelles
Language	English
Publishing date	2021-12-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d1nr04223h
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Self-assembled PEGylated amphiphilic polypeptides for gene transfection

Klemm, Paul / Behnke, Mira / Solomun, Jana I. / Bonduelle, Colin / Lecommandoux, Sébastien / Traeger, Anja / Schubert, Stephanie

Journal of materials chemistry B. 2021 Oct. 13, v. 9, no. 39

2021

Abstract: In the present study, three biodegradable block copolymers composed of a poly(ethylene glycol) block and a copolypeptide block with varying compositions of cationic l-lysine (l-Lys) and hydrophobic benzyl-l-glutamate (Bzl-l-Glu) were designed for gene ... ...

Abstract	In the present study, three biodegradable block copolymers composed of a poly(ethylene glycol) block and a copolypeptide block with varying compositions of cationic l-lysine (l-Lys) and hydrophobic benzyl-l-glutamate (Bzl-l-Glu) were designed for gene delivery applications. The polypeptides were synthesized by ring opening polymerization (ROP) and after orthogonal deprotection of Boc-l-Lys side chains, the polymer exhibited an amphiphilic character. To bind or encapsulate plasmid DNA (pDNA), different formulations were investigated: a nanoprecipitation and an emulsion technique using various organic solvents as well as an aqueous pH-controlled formulation method. The complex and nanoparticle (NP) formations were monitored by dynamic light scattering (DLS), and pDNA interaction was shown by gel electrophoresis and subsequent controlled release with heparin. The polypeptides were further tested for their cytotoxicity as well as biodegradability. The complexes and NPs presenting the most promising size distributions and pDNA binding ability were subsequently evaluated for their transfection efficiency in HEK293T cells. The highest transfection efficiencies were obtained with an aqueous formulation of the polypeptide containing the highest l-Lys content and lowest proportion of hydrophobic, helical structures (P1*), which is therefore a promising candidate for efficient gene delivery by biodegradable gene delivery vectors.
Keywords	biodegradability ; composite polymers ; cytotoxicity ; emulsions ; gel electrophoresis ; genes ; heparin ; hydrophobicity ; lysine ; plasmids ; polymerization ; polypeptides ; transfection
Language	English
Dates of publication	2021-1013
Size	p. 8224-8236.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d1tb01495a
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Manual Versus Microfluidic-Assisted Nanoparticle Manufacture: Impact of Silk Fibroin Stock on Nanoparticle Characteristics.

Solomun, Jana I / Totten, John D / Wongpinyochit, Thidarat / Florence, Alastair J / Seib, F Philipp

ACS biomaterials science & engineering

2020 Volume 6, Issue 5, Page(s) 2796–2804

Abstract: Silk has a long track record of clinical use in the human body, and new formulations, including silk nanoparticles, continue to reveal the promise of this natural biopolymer for healthcare applications. Native silk fibroin can be isolated directly from ... ...

Abstract	Silk has a long track record of clinical use in the human body, and new formulations, including silk nanoparticles, continue to reveal the promise of this natural biopolymer for healthcare applications. Native silk fibroin can be isolated directly from the silk gland, but generating sufficient material for routine studies is difficult. Consequently, silk fibroin, typically extracted from cocoons, serves as the source for nanoparticle formation. This silk requires extensive processing (e.g., degumming, dissolution, etc.) to yield a hypoallergenic aqueous silk stock, but the impact of processing on nanoparticle production and characteristics is largely unknown. Here, manual and microfluidic-assisted silk nanoparticle manufacturing from 60- and 90-min degummed silk yielded consistent particle sizes (100.9-114.1 nm) with low polydispersity. However, the zeta potential was significantly lower (
MeSH term(s)	Fibroins ; Humans ; Microfluidics ; Nanoparticles ; Particle Size ; Silk
Chemical Substances	Silk ; Fibroins (9007-76-5)
Language	English
Publishing date	2020-04-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2373-9878
ISSN	2373-9878
DOI	10.1021/acsbiomaterials.0c00202
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Self-assembled PEGylated amphiphilic polypeptides for gene transfection.

Klemm, Paul / Behnke, Mira / Solomun, Jana I / Bonduelle, Colin / Lecommandoux, Sébastien / Traeger, Anja / Schubert, Stephanie

Journal of materials chemistry. B

2021 Volume 9, Issue 39, Page(s) 8224–8236

Abstract: In the present study, three biodegradable block copolymers composed of a poly(ethylene glycol) block and a copolypeptide block with varying compositions of cationic L-lysine (L-Lys) and hydrophobic benzyl-L-glutamate (Bzl-L-Glu) were designed for gene ... ...

Abstract	In the present study, three biodegradable block copolymers composed of a poly(ethylene glycol) block and a copolypeptide block with varying compositions of cationic L-lysine (L-Lys) and hydrophobic benzyl-L-glutamate (Bzl-L-Glu) were designed for gene delivery applications. The polypeptides were synthesized by ring opening polymerization (ROP) and after orthogonal deprotection of Boc-L-Lys side chains, the polymer exhibited an amphiphilic character. To bind or encapsulate plasmid DNA (pDNA), different formulations were investigated: a nanoprecipitation and an emulsion technique using various organic solvents as well as an aqueous pH-controlled formulation method. The complex and nanoparticle (NP) formations were monitored by dynamic light scattering (DLS), and pDNA interaction was shown by gel electrophoresis and subsequent controlled release with heparin. The polypeptides were further tested for their cytotoxicity as well as biodegradability. The complexes and NPs presenting the most promising size distributions and pDNA binding ability were subsequently evaluated for their transfection efficiency in HEK293T cells. The highest transfection efficiencies were obtained with an aqueous formulation of the polypeptide containing the highest L-Lys content and lowest proportion of hydrophobic, helical structures (P1*), which is therefore a promising candidate for efficient gene delivery by biodegradable gene delivery vectors.
MeSH term(s)	Animals ; Biocompatible Materials/chemistry ; Cell Line ; Cell Survival/drug effects ; DNA/chemistry ; Electrophoresis, Agar Gel ; Gene Transfer Techniques ; Glutamic Acid/analogs & derivatives ; Glutamic Acid/chemistry ; Humans ; Lysine/chemistry ; Mice ; Nanoparticles ; Peptides/chemistry ; Polyethylene Glycols/chemistry ; Transfection
Chemical Substances	Biocompatible Materials ; Peptides ; Glutamic Acid (3KX376GY7L) ; Polyethylene Glycols (3WJQ0SDW1A) ; DNA (9007-49-2) ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2021-10-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d1tb01495a
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The impact of anionic polymers on gene delivery: how composition and assembly help evading the toxicity-efficiency dilemma.

Richter, Friederike / Leer, Katharina / Martin, Liam / Mapfumo, Prosper / Solomun, Jana I / Kuchenbrod, Maren T / Hoeppener, Stephanie / Brendel, Johannes C / Traeger, Anja

Journal of nanobiotechnology

2021 Volume 19, Issue 1, Page(s) 292

Abstract: Cationic polymers have been widely studied for non-viral gene delivery due to their ability to bind genetic material and to interact with cellular membranes. However, their charged nature carries the risk of increased cytotoxicity and interaction with ... ...

Abstract	Cationic polymers have been widely studied for non-viral gene delivery due to their ability to bind genetic material and to interact with cellular membranes. However, their charged nature carries the risk of increased cytotoxicity and interaction with serum proteins, limiting their potential in vivo application. Therefore, hydrophilic or anionic shielding polymers are applied to counteract these effects. Herein, a series of micelle-forming and micelle-shielding polymers were synthesized via RAFT polymerization. The copolymer poly[(n-butyl acrylate)-b-(2-(dimethyl amino)ethyl acrylamide)] (P(nBA-b-DMAEAm)) was assembled into cationic micelles and different shielding polymers were applied, i.e., poly(acrylic acid) (PAA), poly(4-acryloyl morpholine) (PNAM) or P(NAM-b-AA) block copolymer. These systems were compared to a triblock terpolymer micelle comprising PAA as the middle block. The assemblies were investigated regarding their morphology, interaction with pDNA, cytotoxicity, transfection efficiency, polyplex uptake and endosomal escape. The naked cationic micelle exhibited superior transfection efficiency, but increased cytotoxicity. The addition of shielding polymers led to reduced toxicity. In particular, the triblock terpolymer micelle convinced with high cell viability and no significant loss in efficiency. The highest shielding effect was achieved by layering micelles with P(NAM-b-AA) supporting the colloidal stability at neutral zeta potential and completely restoring cell viability while maintaining moderate transfection efficiencies. The high potential of this micelle-layer-combination for gene delivery was illustrated for the first time.
MeSH term(s)	Acrylic Resins ; Animals ; Cations ; Gene Transfer Techniques ; Genetic Therapy ; HEK293 Cells ; Humans ; Hydrophobic and Hydrophilic Interactions ; Mice ; Micelles ; Plasmids ; Polymerization ; Polymers ; Transfection
Chemical Substances	Acrylic Resins ; Cations ; Micelles ; Polymers ; carbopol 940 (4Q93RCW27E)
Language	English
Publishing date	2021-09-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2100022-0
ISSN	1477-3155 ; 1477-3155
ISSN (online)	1477-3155
ISSN	1477-3155
DOI	10.1186/s12951-021-00994-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: pH-sensitive packaging of cationic particles by an anionic block copolymer shell.

Solomun, Jana I / Martin, Liam / Mapfumo, Prosper / Moek, Elisabeth / Amro, Elias / Becker, Friedrich / Tuempel, Stefan / Hoeppener, Stephanie / Rudolph, K Lenhard / Traeger, Anja

Journal of nanobiotechnology

2022 Volume 20, Issue 1, Page(s) 336

Abstract: Cationic non-viral vectors show great potential to introduce genetic material into cells, due to their ability to transport large amounts of genetic material and their high synthetic versatility. However, designing materials that are effective without ... ...

Abstract	Cationic non-viral vectors show great potential to introduce genetic material into cells, due to their ability to transport large amounts of genetic material and their high synthetic versatility. However, designing materials that are effective without showing toxic effects or undergoing non-specific interactions when applied systemically remains a challenge. The introduction of shielding polymers such as polyethylene glycol (PEG) can enhance biocompatibility and circulation time, however, often impairs transfection efficiency. Herein, a multicomponent polymer system is introduced, based on cationic and hydrophobic particles (P(nBMA
MeSH term(s)	Animals ; Cations ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; Mice ; Polyethylene Glycols/chemistry ; Polymers/chemistry ; Transfection
Chemical Substances	Cations ; Polymers ; Polyethylene Glycols (3WJQ0SDW1A)
Language	English
Publishing date	2022-07-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2100022-0
ISSN	1477-3155 ; 1477-3155
ISSN (online)	1477-3155
ISSN	1477-3155
DOI	10.1186/s12951-022-01528-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Efficient Gene Delivery of Tailored Amphiphilic Polypeptides by Polyplex Surfing.

Klemm, Paul / Solomun, Jana I / Rodewald, Marko / Kuchenbrod, Maren T / Hänsch, Veit G / Richter, Friederike / Popp, Jürgen / Hertweck, Christian / Hoeppener, Stephanie / Bonduelle, Colin / Lecommandoux, Sébastien / Traeger, Anja / Schubert, Stephanie

Biomacromolecules

2022 Volume 23, Issue 11, Page(s) 4718–4733

Abstract: Within this study, an amphiphilic and potentially biodegradable polypeptide library based on poly[(4-aminobutyl)-l-glutamine- ...

Abstract	Within this study, an amphiphilic and potentially biodegradable polypeptide library based on poly[(4-aminobutyl)-l-glutamine-
MeSH term(s)	Glutamine ; Gene Transfer Techniques ; Genetic Therapy ; Transfection ; Cations ; Peptides
Chemical Substances	Glutamine (0RH81L854J) ; Cations ; Peptides
Language	English
Publishing date	2022-10-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.2c00919
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED