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Artikel ; Online: Minichromosome maintenance 6 protects against renal fibrogenesis by regulating DUSP6-mediated ERK/GSK-3β/Snail1 signaling.

Huang, Jing / Xu, Zhi-Feng / Liu, Feng / Song, An-Ni / Su, Hua / Zhang, Chun

iScience

2023 Band 26, Heft 10, Seite(n) 107940

Abstract: Minichromosome maintenance 6 (MCM6) has been implicated in the progression of various malignant tumors; however, its exact physiological function in kidney diseases remains unclear. Here, we demonstrated that MCM6 levels showed a significant increase in ... ...

Abstract	Minichromosome maintenance 6 (MCM6) has been implicated in the progression of various malignant tumors; however, its exact physiological function in kidney diseases remains unclear. Here, we demonstrated that MCM6 levels showed a significant increase in the proximal tubular cells during progressive renal fibrosis in two unrelated
Sprache	Englisch
Erscheinungsdatum	2023-09-16
Erscheinungsland	United States
Dokumenttyp	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2023.107940
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Application of diversified teaching in the clinical teaching of Endocrinology Department for Eight-year Medical Program students

SONG An / Li Ran / LIU Yiwen / ZHOU Xiang / DUAN Lian / WANG Ou / LI Yuxiu

Jichu yixue yu linchuang, Vol 43, Iss 10, Pp 1620-

2023 Band 1624

Abstract: Objective To explore the influence of different teaching strategies on the clinical teaching effect of Eight-year Medical Program students in Endocrinology Department. Methods This study conducted an online questionnaire for evaluation of the teaching ... ...

Abstract	Objective To explore the influence of different teaching strategies on the clinical teaching effect of Eight-year Medical Program students in Endocrinology Department. Methods This study conducted an online questionnaire for evaluation of the teaching quality of training for attending doctors, residents and the effect of small lectures of medical students. Participants were students from 6th-8th grades of Eight-year Program of clinical medicine in Peking Union Medical Colleges Hospital, rotating in the Department of Endocrinology from December 2021 to April 2022. Results Questionnaires were distributed to 48 medical students who had completed the rotation of Endocrinology Department. A total of 40 valid questionnaires were recovered with a recovery rate of 83.3%. The attending doctors and residents in the ward performed well in the daily teaching activities. The busy clinical work was a main factor affecting the teaching quality. Most medical students prepared lectures within 6 hours. In addition to mastering relevant knowledge, lectures also improved the speech ability, literature retrieval ability and slide production ability of students. Conclusions Adopting diverse teaching strategies and organizing various forms of traning activity are conducive to arouse the learning enthusiasm of medical students and comprehensively improve their post competencies.
Schlagwörter	department of endocrinology\|diversified teaching strategies\|eight-year medical program\|teaching quality\|questionnaire assessment ; Medicine ; R
Thema/Rubrik (Code)	370
Sprache	Chinesisch
Erscheinungsdatum	2023-10-01T00:00:00Z
Verlag	Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: MDM2 accelerated renal senescence via ubiquitination and degradation of HDAC1.

Xiang, Hui-Ling / Yuan, Qian / Zeng, Jie-Yu / Xu, Zi-Yu / Zhang, Hui-Zi / Huang, Jing / Song, An-Ni / Xiong, Jing / Zhang, Chun

Acta pharmacologica Sinica

2024

Abstract: Senescence, an intricate and inevitable biological process, characterized by the gradual loss of homeostasis and declining organ functions. The pathological features of cellular senescence, including cell cycle arrest, metabolic disruptions, and the ... ...

Abstract	Senescence, an intricate and inevitable biological process, characterized by the gradual loss of homeostasis and declining organ functions. The pathological features of cellular senescence, including cell cycle arrest, metabolic disruptions, and the emergence of senescence-associated secretory phenotypes (SASP), collectively contribute to the intricate and multifaceted nature of senescence. Beyond its classical interaction with p53, murine double minute gene 2 (MDM2), traditionally known as an E3 ubiquitin ligase involved in protein degradation, plays a pivotal role in cellular processes governing senescence. Histone deacetylase (HDAC), a class of histone deacetylases mainly expressed in the nucleus, has emerged as a critical contributor to renal tissues senescence. In this study we investigated the interplay between MDM2 and HDAC1 in renal senescence. We established a natural aging model in mice over a 2-year period that was verified by SA-β-GAL staining and increased expression of senescence-associated markers such as p21, p16, and TNF-α in the kidneys. Furthermore, we showed that the expression of MDM2 was markedly increased, while HDAC1 expression underwent downregulation during renal senescence. This phenomenon was confirmed in H
Sprache	Englisch
Erscheinungsdatum	2024-05-17
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	1360774-1
ISSN	1745-7254 ; 0253-9756 ; 1671-4083
ISSN (online)	1745-7254
ISSN	0253-9756 ; 1671-4083
DOI	10.1038/s41401-024-01294-9
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Inhibition of MAD2B alleviates venous neointimal formation by suppressing VSMCs proliferation and migration.

Song, An-Ni / Yin, Xing-Jie / Gao, Pan / Tang, Hui / Meng, Xian-Fang / Zhang, Chun

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2021 Band 35, Heft 11, Seite(n) e21959

Abstract: The proliferation and migration of vascular smooth muscle cells (VSMCs) are essential events in venous neointimal hyperplasia (VNH), a culprit of arteriovenous fistula (AVF) malfunction. Mitotic arrest-deficient protein 2B (MAD2B) is a critical regulator ...

Abstract	The proliferation and migration of vascular smooth muscle cells (VSMCs) are essential events in venous neointimal hyperplasia (VNH), a culprit of arteriovenous fistula (AVF) malfunction. Mitotic arrest-deficient protein 2B (MAD2B) is a critical regulator of cell proliferation and differentiation in many scenarios. To address the role of MAD2B in VSMCs proliferation and migration during VNH, AVFs from patients with end-stage renal disease (ESRD) and chronic kidney disease (CKD) mice were used to evaluate MAD2B expression. In cultured VSMCs treated with platelet-derived growth factor-BB (PDGF-BB), the effect of MAD2B on VSMCs proliferation and migration was detected by cell counting kit-8 (CCK8) assay, immunofluorescence, wound-healing scratch and transwell assays. Besides, we exploited different small interfering RNAs (siRNAs) to explore the potential mechanisms in the issue. Furthermore, rapamycin was applied to reveal whether MAD2B-associated pathways were involved in its inhibitory effect on VSMCs proliferation and migration. Accordingly, we found that MAD2B expression was enhanced in AVFs from patients with ESRD, CKD mice and VSMCs stimulated by PDGF-BB. Meanwhile, inhibition of MAD2B alleviated VSMCs proliferation and migration while the number of ski-related novel gene (SnoN)-positive VSMCs was also increased in vivo and in vitro. Moreover, gene deletion of MAD2B decreased the level of SnoN protein in PDGF-BB-stimulated VSMCs. Furthermore, rapamycin suppressed the increased expressions of MAD2B and SnoN induced by PDGF-BB. Thus, our study demonstrates that inhibition of MAD2B suppresses the proliferation and migration of VSMCs during VNH via reducing SnoN expression. Moreover, rapamycin exerts an inhibitory effect on intimal hyperplasia, possibly via the MAD2B-SnoN axis.
Mesh-Begriff(e)	Animals ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Humans ; Hyperplasia/metabolism ; Hyperplasia/pathology ; Kidney Failure, Chronic/metabolism ; Mad2 Proteins/physiology ; Male ; Mice ; Mice, Inbred C57BL ; Muscle, Smooth, Vascular/metabolism ; Muscle, Smooth, Vascular/pathology ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/pathology ; Neointima/metabolism ; Neointima/pathology
Chemische Substanzen	MAD2L2 protein, human ; Mad2 Proteins ; Mad2l2 protein, mouse
Sprache	Englisch
Erscheinungsdatum	2021-10-04
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.202100584RR
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Analysis of Significant Genes and Pathways in Esophageal Cancer Based on Gene Expression Omnibus Database.

Song, An-Yi / Mu, Lan / Dai, Xiao-Yong / Wang, Li-Jun / Huang, Lai-Qiang

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih

2023 Band 38, Heft 1, Seite(n) 20–28

Abstract: Objective To screen antigen targets for immunotherapy by analyzing over-expressed genes, and to identify significant pathways and molecular mechanisms in esophageal cancer by using bioinformatic methods such as enrichment analysis, protein-protein ... ...

Abstract	Objective To screen antigen targets for immunotherapy by analyzing over-expressed genes, and to identify significant pathways and molecular mechanisms in esophageal cancer by using bioinformatic methods such as enrichment analysis, protein-protein interaction (PPI) network, and survival analysis based on the Gene Expression Omnibus (GEO) database.Methods By screening with highly expressed genes, we mainly analyzed proteins MUC13 and EPCAM with transmembrane domain and antigen epitope from TMHMM and IEDB websites. Significant genes and pathways associated with the pathogenesis of esophageal cancer were identified using enrichment analysis, PPI network, and survival analysis. Several software and platforms including Prism 8, R language, Cytoscape, DAVID, STRING, and GEPIA platform were used in the search and/or figure creation.Results Genes
Mesh-Begriff(e)	Humans ; Esophageal Neoplasms/genetics ; Esophageal Neoplasms/metabolism ; Esophageal Squamous Cell Carcinoma/genetics ; Esophageal Squamous Cell Carcinoma/metabolism ; Epithelial Cell Adhesion Molecule/genetics ; Epithelial Cell Adhesion Molecule/metabolism ; Gene Expression Profiling/methods ; Gene Regulatory Networks ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Intracellular Signaling Peptides and Proteins
Chemische Substanzen	Epithelial Cell Adhesion Molecule ; ZBTB7C protein, human ; Intracellular Signaling Peptides and Proteins
Sprache	Englisch
Erscheinungsdatum	2023-02-28
Erscheinungsland	China
Dokumenttyp	Journal Article
ZDB-ID	1083365-1
ISSN	1001-9294
ISSN	1001-9294
DOI	10.24920/004148
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Rationally designed nanotrap structures for efficient separation of rare earth elements over a single step.

Hu, Qing-Hua / Song, An-Min / Gao, Xin / Shi, Yu-Zhen / Jiang, Wei / Liang, Ru-Ping / Qiu, Jian-Ding

Nature communications

2024 Band 15, Heft 1, Seite(n) 1558

Abstract: Extracting rare earth elements (REEs) from wastewater is essential for the growth and an eco-friendly sustainable economy. However, it is a daunting challenge to separate individual rare earth elements by their subtle differences. To overcome this ... ...

Abstract	Extracting rare earth elements (REEs) from wastewater is essential for the growth and an eco-friendly sustainable economy. However, it is a daunting challenge to separate individual rare earth elements by their subtle differences. To overcome this difficulty, we report a unique REE nanotrap that features dense uncoordinated carboxyl groups and triazole N atoms in a two-fold interpenetrated metal-organic framework (named NCU-1). Notably, the synergistic effect of suitable pore sizes and REE nanotraps in NCU-1 is highly responsive to the size variation of rare-earth ions and shows high selectivity toward light REE. As a proof of concept, Pr/Lu and Nd/Er are used as binary models, which give a high separation factor of SF
Sprache	Englisch
Erscheinungsdatum	2024-02-20
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45810-1
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Topological disruption of low- and high-order functional networks in presbycusis.

Xu, Yixi / Li, Xiangxiang / Yan, Qi / Zhang, Yao / Shang, Song'an / Xing, Chunhua / Wu, Yuanqing / Guan, Bing / Chen, Yu-Chen

Brain communications

2024 Band 6, Heft 2, Seite(n) fcae119

Abstract: Prior efforts have manifested that functional connectivity (FC) network disruptions are concerned with cognitive disorder in presbycusis. The present research was designed to investigate the topological reorganization and classification performance of ... ...

Abstract	Prior efforts have manifested that functional connectivity (FC) network disruptions are concerned with cognitive disorder in presbycusis. The present research was designed to investigate the topological reorganization and classification performance of low-order functional connectivity (LOFC) and high-order functional connectivity (HOFC) networks in patients with presbycusis. Resting-state functional magnetic resonance imaging (Rs-fMRI) data were obtained in 60 patients with presbycusis and 50 matched healthy control subjects (HCs). LOFC and HOFC networks were then constructed, and the topological metrics obtained from the constructed networks were compared to evaluate topological differences in global, nodal network metrics, modularity and rich-club organization between patients with presbycusis and HCs. The use of HOFC profiles boosted presbycusis classification accuracy, sensitivity and specificity compared to that using LOFC profiles. The brain networks in both patients with presbycusis and HCs exhibited small-world properties within the given threshold range, and striking differences between groups in topological metrics were discovered in the constructed networks (LOFC and HOFC). NBS analysis identified a subnetwork involving 26 nodes and 23 signally altered internodal connections in patients with presbycusis in comparison to HCs in HOFC networks. This study highlighted the topological differences between LOFC and HOFC networks in patients with presbycusis, suggesting that HOFC profiles may help to further identify brain network abnormalities in presbycusis.
Sprache	Englisch
Erscheinungsdatum	2024-04-06
Erscheinungsland	England
Dokumenttyp	Journal Article
ISSN	2632-1297
ISSN (online)	2632-1297
DOI	10.1093/braincomms/fcae119
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: m

Li, Shujin / Feng, Tian / Liu, Yuantong / Yang, Qichao / Song, An / Wang, Shuo / Xie, Jun / Zhang, Junjie / Yuan, Bifeng / Sun, Zhijun

International journal of oral science

2024 Band 16, Heft 1, Seite(n) 36

Abstract: ... ...

Abstract	N
Mesh-Begriff(e)	Animals ; B7-H1 Antigen ; Mice ; Proto-Oncogene Proteins c-myc/metabolism ; Oncolytic Viruses ; Signal Transduction ; Humans ; Adenosine/analogs & derivatives ; Down-Regulation ; Squamous Cell Carcinoma of Head and Neck/immunology ; Squamous Cell Carcinoma of Head and Neck/therapy ; Oncolytic Virotherapy/methods ; PTEN Phosphohydrolase ; Mice, Knockout ; Head and Neck Neoplasms/immunology ; Head and Neck Neoplasms/therapy ; Simplexvirus ; Cell Line, Tumor
Chemische Substanzen	B7-H1 Antigen ; Proto-Oncogene Proteins c-myc ; Adenosine (K72T3FS567) ; N-methyladenosine (CLE6G00625) ; PTEN Phosphohydrolase (EC 3.1.3.67) ; Cd274 protein, mouse ; Myc protein, mouse
Sprache	Englisch
Erscheinungsdatum	2024-05-10
Erscheinungsland	India
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2569849-7
ISSN	2049-3169 ; 1674-2818
ISSN (online)	2049-3169
ISSN	1674-2818
DOI	10.1038/s41368-024-00304-0
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Intracerebral hemodynamic abnormalities in patients with Parkinson's disease: Comparison between multi-delay arterial spin labelling and conventional single-delay arterial spin labelling.

Wang, Xue / Wang, Lijuan / Wu, Yating / Lv, Xiang / Xu, Yao / Dou, Weiqiang / Zhang, Hongying / Wu, Jingtao / Shang, Song'an

Diagnostic and interventional imaging

2024

Abstract: Purpose: The purpose of this study was to analyze the intracerebral abnormalities of hemodynamics in patients with Parkinson's disease (PD) through arterial spin labelling (ASL) technique with multi-delay ASL (MDASL) and conventional single-delay ASL ( ... ...

Abstract	Purpose: The purpose of this study was to analyze the intracerebral abnormalities of hemodynamics in patients with Parkinson's disease (PD) through arterial spin labelling (ASL) technique with multi-delay ASL (MDASL) and conventional single-delay ASL (SDASL) protocols and to verify the potential clinical application of these features for the diagnosis of PD. Materials and methods: Perfusion data of the brain obtained using MDASL and SDASL in patients with PD were compared to those obtained in healthy control (HC) subjects. Intergroup comparisons of z-scored cerebral blood flow (zCBF), arterial transit time (zATT) and cerebral blood volume (zCBV) were performed via voxel-based analysis. Performance of these perfusion metrics were estimated using area under the receiver operating characteristic curve (AUC) and compared using Delong test. Results: A total of 47 patients with PD (29 men; 18 women; mean age, 69.0 ± 7.6 (standard deviation, [SD]) years; range: 50.0-84.0 years) and 50 HC subjects (28 men; 22 women; mean age, 70.1 ± 6.2 [SD] years; range: 50.0-93.0 years) were included. Relative to the uncorrected-zCBF map, the corrected-zCBF map further refined the distributed brain regions in the PD group versus the HC group, manifested as the extension of motor-related regions (P Conclusion: ASL identifies impaired hemodynamics in patients with PD including regional abnormalities of CBF, CBV and ATT, which can better be mapped with MDASL compared to SDASL. These findings provide complementary depictions of perfusion abnormalities in patients with PD and highlight the clinical feasibility of MDASL.
Sprache	Englisch
Erscheinungsdatum	2024-02-02
Erscheinungsland	France
Dokumenttyp	Journal Article
ZDB-ID	2648283-6
ISSN	2211-5684 ; 2211-5684
ISSN (online)	2211-5684
ISSN	2211-5684
DOI	10.1016/j.diii.2024.01.006
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Clinical Effect of Side-Approach Laparoscopic Splenectomy for Neuropathic Splenomegaly

Zhao Yuan / Hua Song An / Bo Yang / Hai Qing Li / Yuan Wang / Jian Wang

Computational and Mathematical Methods in Medicine, Vol

2022 Band 2022

Abstract: Objective. In order to investigate the clinical effect of side entry laparoscopic splenectomy in the treatment of splenomegaly caused by neurodegenerative diseases. Methods. 62 patients who underwent endoscopic splenectomy in our hospital from July 2020 ... ...

Abstract	Objective. In order to investigate the clinical effect of side entry laparoscopic splenectomy in the treatment of splenomegaly caused by neurodegenerative diseases. Methods. 62 patients who underwent endoscopic splenectomy in our hospital from July 2020 to June 2021 were randomly divided into two groups, including 3 cases in the observation group and 31 cases in the reference group. Clinical trials were conducted to compare different laparoscopic surgery methods, and follow-up investigation records were made; the drainage time and drainage volume, postoperative bleeding volume, postoperative complications, and comprehensive effective rate of the two groups were observed and analyzed. Results. Most of the drainage volume in the observation group was less than 800 ml, and most of the drainage volume in the reference group was more than 500 ml. Compared with the reference group, the average drainage time of patients in the observation group was lower, mostly within 6 days, while the drainage time of patients in the reference group was more than 8 days. The amount of bleeding in the observation group was mostly about 500 ml, with the largest number in the range of 300-500 ml, while the amount of bleeding in the reference group was mostly 800 ml and above, with the largest number in the range of 500-800 ml. The incidence of complications in the observation group was lower than that in the reference group. The effective, markedly effective and comprehensive effective rates of patients in the observation group were higher than those in the reference group, and the ineffective rate and deterioration rate were also lower. Conclusion. The treatment of lateral laparoscopic splenectomy is very safe and effective and has obvious advantages, because it can reduce the occurrence of postoperative complications and provide a good basis for the recovery of patients. It is worthy to be widely used in clinical splenic surgery.
Schlagwörter	Computer applications to medicine. Medical informatics ; R858-859.7
Thema/Rubrik (Code)	610
Sprache	Englisch
Erscheinungsdatum	2022-01-01T00:00:00Z
Verlag	Hindawi Limited
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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