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Article ; Online: Stepwise transmigration of T- and B cells through a perivascular channel in high endothelial venules.

Choe, Kibaek / Moon, Jieun / Lee, Soo Yun / Song, Eunjoo / Back, Ju Hee / Song, Joo-Hye / Hyun, Young-Min / Uchimura, Kenji / Kim, Pilhan

Life science alliance

2021 Volume 4, Issue 8

Abstract: High endothelial venules (HEVs) effectively recruit circulating lymphocytes from the blood to lymph nodes. HEVs have endothelial cells (ECs) and perivascular sheaths consisting of fibroblastic reticular cells (FRCs). Yet, post-luminal lymphocyte ... ...

Abstract	High endothelial venules (HEVs) effectively recruit circulating lymphocytes from the blood to lymph nodes. HEVs have endothelial cells (ECs) and perivascular sheaths consisting of fibroblastic reticular cells (FRCs). Yet, post-luminal lymphocyte migration steps are not well elucidated. Herein, we performed intravital imaging to investigate post-luminal T- and B-cell migration in popliteal lymph node, consisting of trans-EC migration, crawling in the perivascular channel (a narrow space between ECs and FRCs) and trans-FRC migration. The post-luminal migration of T cells occurred in a PNAd-dependent manner. Remarkably, we found hot spots for the trans-EC and trans-FRC migration of T- and B cells. Interestingly, T- and B cells preferentially shared trans-FRC migration hot spots but not trans-EC migration hot spots. Furthermore, the trans-FRC T-cell migration was confined to fewer sites than trans-EC T-cell migration, and trans-FRC migration of T- and B cells preferentially occurred at FRCs covered by CD11c+ dendritic cells in HEVs. These results suggest that HEV ECs and FRCs with perivascular DCs delicately regulate T- and B-cell entry into peripheral lymph nodes.
MeSH term(s)	Animals ; B-Lymphocytes/metabolism ; Intravital Microscopy ; Lymph Nodes/immunology ; Mice ; T-Lymphocytes/metabolism ; Transendothelial and Transepithelial Migration ; Venules/immunology
Language	English
Publishing date	2021-06-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2575-1077
ISSN (online)	2575-1077
DOI	10.26508/lsa.202101086
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Two distinct receptor-binding domains of human glycyl-tRNA synthetase 1 displayed on extracellular vesicles activate M1 polarization and phagocytic bridging of macrophages to cancer cells.

Park, Min Chul / Goughnour, Peter C / Jun, Sangmi / Cho, Seongmin / Song, Eunjoo / Kim, Sang Bum / Kim, Hyeong Yun / Hyun, Jae Kyung / Kim, Pilhan / Jung, Hyun Suk / Kim, Sunghoon

Cancer letters

2022 Volume 539, Page(s) 215698

Abstract: Macrophages play important roles in cancer microenvironment. Human cytosolic glycyl-tRNA synthetase (GARS1) was previously shown to be secreted via extracellular vesicles (EVs) from macrophages to trigger cancer cell death. However, the effects of GARS1- ... ...

Abstract	Macrophages play important roles in cancer microenvironment. Human cytosolic glycyl-tRNA synthetase (GARS1) was previously shown to be secreted via extracellular vesicles (EVs) from macrophages to trigger cancer cell death. However, the effects of GARS1-containing EVs (GARS1-EVs) on macrophages as well as on cancer cells and the working mechanisms of GARS1 in cancer microenvironment are not yet understood. Here we show that GARS1-EVs induce M1 polarization and facilitate phagocytosis of macrophages. GARS1-EVs triggers M1 polarization of macrophage via the specific interaction of the extracellular cadherin subdomains 1-4 of the cadherin EGF LAG seven-pass G-type receptor 2 (CELSR2) with the N-terminal WHEP domain containing peptide region of GARS1, and activates the RAF-MEK-ERK pathway for M1 type cytokine production and phagocytosis. Besides, GARS1 interacted with cadherin 6 (CDH6) of cancer cells via its C-terminal tRNA-binding domain to induce cancer cell death. In vivo model, GARS1-EVs showed potent suppressive activity against tumor initiation via M1 type macrophages. GARS1 displayed on macrophage-secreted extracellular vesicles suppressed tumor growth in dual mode, namely through pro-apoptotic effect on cancer cells and M1 polarization effect on macrophages. Collectively, these results elucidate the unique tumor suppressive activity and mechanism of GARS1-EVs by activating M1 macrophage via CELSR2 as well as by direct killing of cancer cells via CDH6.
MeSH term(s)	Cadherins/metabolism ; Cell Polarity ; Extracellular Vesicles/enzymology ; Extracellular Vesicles/metabolism ; Glycine-tRNA Ligase/analysis ; Glycine-tRNA Ligase/metabolism ; Glycine-tRNA Ligase/pharmacology ; Humans ; Macrophages/enzymology ; Macrophages/metabolism ; Macrophages/pathology ; Neoplasms/enzymology ; Neoplasms/metabolism ; Phagocytosis ; Tumor Microenvironment
Chemical Substances	Cadherins ; Glycine-tRNA Ligase (EC 6.1.1.14)
Language	English
Publishing date	2022-05-09
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	195674-7
ISSN	1872-7980 ; 0304-3835
ISSN (online)	1872-7980
ISSN	0304-3835
DOI	10.1016/j.canlet.2022.215698
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Article: Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging.

Lee, Dongwoo / Kim, Jihye / Song, Eunjoo / Jeong, Ji-Young / Jeon, Eun-Chae / Kim, Pilhan / Lee, Wonhee

Micromachines

2020 Volume 11, Issue 6

Abstract: 3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex ... ...

Abstract	3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures.
Language	English
Publishing date	2020-06-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2620864-7
ISSN	2072-666X
ISSN	2072-666X
DOI	10.3390/mi11060582
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Article: In vivo

Ahn, Jinhyo / Kong, Eunji / Choe, Kibaek / Song, Eunjoo / Hwang, Yoonha / Seo, Howon / Park, Inwon / Kim, Pilhan

Biomedical optics express

2019 Volume 10, Issue 6, Page(s) 2719–2729

Abstract: ... In ... ...

Abstract	In vivo
Language	English
Publishing date	2019-05-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.10.002719
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Article: Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model.

Park, Inwon / Choe, Kibaek / Seo, Howon / Hwang, Yoonha / Song, Eunjoo / Ahn, Jinhyo / Hwan Jo, You / Kim, Pilhan

Biomedical optics express

2018 Volume 9, Issue 5, Page(s) 2383–2393

Abstract: Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role of a vascular endothelial barrier in various pathological conditions. Despite its importance as a marker of ...

Abstract	Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role of a vascular endothelial barrier in various pathological conditions. Despite its importance as a marker of endothelial cell damage and impairment of the vascular system,
Language	English
Publishing date	2018-04-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.9.002383
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Article: Synthesis, stability and electrical properties of new soluble pentacenes with unsaturated side groups

Kim, Heung Gyu / Cho, Kilwon / Choi, E-Joon / Choi, Hyun Ho / Song, Eunjoo

RSC advances. 2015 Jan. 06, v. 5, no. 11

2015

Abstract: A series of pentacene derivatives with unsaturated hydrocarbon substituents at the 6,13-positions as side groups have been synthesized. The effects of conjugated π-bonding throughout the side groups on the solubility, oxidative stability, crystallinity, ... ...

Abstract	A series of pentacene derivatives with unsaturated hydrocarbon substituents at the 6,13-positions as side groups have been synthesized. The effects of conjugated π-bonding throughout the side groups on the solubility, oxidative stability, crystallinity, and electrical properties of the compounds were investigated. Here we report that the solubility of pentacene can be greatly improved by the insertion of linear long side groups at the 6,13-positions: the obtained pentacene derivatives were soluble in common organic solvents. Time-dependent UV-visible spectra in chloroform solutions as well as thin films were recorded to investigate the oxidative stability of each pentacene compound under ambient conditions. The photooxidative stability of the pentacene derivatives was remarkably enhanced by increasing the electron conjugation length through the installation of unsaturated hydrocarbon substituents at the 6,13-positions. The solution-processed TFT devices based on 6,13-bis(4-pentylphenyl-ethynyl)pentacene (P5) and 6,13-bis(4-hexylphenylethynyl)pentacene (P6) showed charge transport mobilities of 2.14 × 10−2 cm2 V−1 s−1 and 3.94 × 10−2 cm2 V−1 s−1, respectively, and exhibited an on/off ratio of 105. Here we demonstrate that P5 and P6 revealed much more stable electrical performances than the parent pentacene under the oxidative environment.
Keywords	chloroform ; crystal structure ; electrical properties ; films (materials) ; oxidative stability ; photooxidation ; solubility ; solvents
Language	English
Dates of publication	2015-0106
Size	p. 8070-8076.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c4ra11195h
Database	NAL-Catalogue (AGRICOLA)

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Article: Naphthodithiophene-Based Conjugated Polymer with Linear, Planar Backbone Conformation and Strong Intermolecular Packing for Efficient Organic Solar Cells

Lee, Jaewon / Cho Kilwon / Kim Heung Gyu / Ko Hyomin / Song Eunjoo

ACS Applied Materials & Interfaces. 2015 Sept. 30, v. 7, no. 38

2015

Abstract: Two donorâacceptor copolymers, PBDT and PNDT, containing 4,8-bis(2-ethylhexyloxy)benzo[1,2-b:3,4-bâ²]dithiophene (BDT) and 4,9-bis(2-ethylhexyloxy)naphtho[1,2-b:5,6-bâ²]dithiophene (NDT), respectively, as an electron-rich unit and 5,6-difluoro-2,1,3- ... ...

Abstract	Two donorâacceptor copolymers, PBDT and PNDT, containing 4,8-bis(2-ethylhexyloxy)benzo[1,2-b:3,4-bâ²]dithiophene (BDT) and 4,9-bis(2-ethylhexyloxy)naphtho[1,2-b:5,6-bâ²]dithiophene (NDT), respectively, as an electron-rich unit and 5,6-difluoro-2,1,3-benzothiadiazole (2FBT) as an electron-deficient unit, were synthesized and compared. The introduction of the NDT core into the conjugated backbone was found to effectively improve both light harvesting and the charge carrier mobility by enhancing chain planarity and backbone linearity; the NDT copolymer has stronger noncovalent interactions and smaller bond angles than those of the BDT-based polymer. Moreover, the introduction of the NDT core brings about a drastic change in the molecular orientation into the face-on motif and results in polymer:PCBM blend films with well-mixed interpenetrating nanofibrillar bulkâheterojunction networks with small-scale phase separation, which produce solar cells with higher short-circuit current density and fill factor values. A conventional optimized device structure containing PNDT:PCââBM was found to exhibit a maximum solar efficiency of 6.35%, an open-circuit voltage of 0.84 V, a short-circuit current density of 11.92 mA cmâÂ², and a fill factor of 63.5% with thermal annealing, which demonstrates that the NDT and DT2FBT moieties are a promising electron-donor/acceptor combination for high-performance photovoltaics.
Keywords	chemical structure ; composite polymers ; photovoltaic cells ; solar energy ; thiophenes
Language	English
Dates of publication	2015-0930
Size	p. 21159-21169.
Publishing place	American Chemical Society
Document type	Article
ISSN	1944-8252
DOI	10.1021%2Facsami.5b04884
Database	NAL-Catalogue (AGRICOLA)

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Article: Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models.

Park, Inwon / Hong, Sujung / Seok, Joon / Lucia, Stephani Edwina / Song, Eunjoo / Kim, Mingyo / Kong, Eunji / Seo, Howon / Hwang, Yoonha / Ahn, Soyeon / Kim, Seonghye / Jang, Dong-Hyun / Lee, Jae Hyuk / Park, Su-Hyung / Kim, Pilhan / Jo, You Hwan

Journal of breast cancer

2021 Volume 24, Issue 5, Page(s) 463–473

Abstract: Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate the spatiotemporal cellular dynamics of TILs in breast cancer models. Breast ... ...

Abstract	Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate the spatiotemporal cellular dynamics of TILs in breast cancer models. Breast cancer cells were implanted into the dorsal skinfold chamber of BALB/c nude mice, and T lymphocytes were adoptively transferred. Longitudinal intravital imaging was performed, and the spatiotemporal dynamics of TILs were assessed. In the 4T1 model, TILs progressively exhibited increased motility, and their motility inside the tumor was significantly higher than that outside the tumor. In the MDA-MB-231 model, the motility of TILs progressively decreased after an initial increase. TIL motility in the MDA-MB-231 and MCF-7 models differed significantly, suggesting an association between programmed death-ligand 1 expression levels and TIL motility, which warrants further investigation. Furthermore, intravital imaging of TILs can be a useful method for addressing dynamic interactions between TILs and breast cancer cells.
Language	English
Publishing date	2021-09-08
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2559753-X
ISSN	2092-9900 ; 1738-6756
ISSN (online)	2092-9900
ISSN	1738-6756
DOI	10.4048/jbc.2021.24.e40
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Zs.A 6866: Show issues

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Article: Optical clearing based cellular-level 3D visualization of intact lymph node cortex.

Song, Eunjoo / Seo, Howon / Choe, Kibaek / Hwang, Yoonha / Ahn, Jinhyo / Ahn, Soyeon / Kim, Pilhan

Biomedical optics express

2015 Volume 6, Issue 10, Page(s) 4154–4164

Abstract: Lymph node (LN) is an important immune organ that controls adaptive immune responses against foreign pathogens and abnormal cells. To facilitate efficient immune function, LN has highly organized 3D cellular structures, vascular and lymphatic system. ... ...

Abstract	Lymph node (LN) is an important immune organ that controls adaptive immune responses against foreign pathogens and abnormal cells. To facilitate efficient immune function, LN has highly organized 3D cellular structures, vascular and lymphatic system. Unfortunately, conventional histological analysis relying on thin-sliced tissue has limitations in 3D cellular analysis due to structural disruption and tissue loss in the processes of fixation and tissue slicing. Optical sectioning confocal microscopy has been utilized to analyze 3D structure of intact LN tissue without physical tissue slicing. However, light scattering within biological tissues limits the imaging depth only to superficial portion of LN cortex. Recently, optical clearing techniques have shown enhancement of imaging depth in various biological tissues, but their efficacy for LN are remained to be investigated. In this work, we established optical clearing procedure for LN and achieved 3D volumetric visualization of the whole cortex of LN. More than 4 times improvement in imaging depth was confirmed by using LN obtained from H2B-GFP/actin-DsRed double reporter transgenic mouse. With adoptive transfer of GFP expressing B cells and DsRed expressing T cells and fluorescent vascular labeling by anti-CD31 and anti-LYVE-1 antibody conjugates, we successfully visualized major cellular-level structures such as T-cell zone, B-cell follicle and germinal center. Further, we visualized the GFP expressing metastatic melanoma cell colony, vasculature and lymphatic vessels in the LN cortex.
Language	English
Publishing date	2015-09-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.6.004154
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Article ; Online: Naphthodithiophene-Based Conjugated Polymer with Linear, Planar Backbone Conformation and Strong Intermolecular Packing for Efficient Organic Solar Cells.

Lee, Jaewon / Ko, Hyomin / Song, Eunjoo / Kim, Heung Gyu / Cho, Kilwon

ACS applied materials & interfaces

2015 Volume 7, Issue 38, Page(s) 21159–21169

Abstract: Two donor-acceptor copolymers, PBDT and PNDT, containing 4,8-bis(2-ethylhexyloxy)benzo[1,2-b:3,4-b']dithiophene (BDT) and 4,9-bis(2-ethylhexyloxy)naphtho[1,2-b:5,6-b']dithiophene (NDT), respectively, as an electron-rich unit and 5,6-difluoro-2,1,3- ... ...

Abstract	Two donor-acceptor copolymers, PBDT and PNDT, containing 4,8-bis(2-ethylhexyloxy)benzo[1,2-b:3,4-b']dithiophene (BDT) and 4,9-bis(2-ethylhexyloxy)naphtho[1,2-b:5,6-b']dithiophene (NDT), respectively, as an electron-rich unit and 5,6-difluoro-2,1,3-benzothiadiazole (2FBT) as an electron-deficient unit, were synthesized and compared. The introduction of the NDT core into the conjugated backbone was found to effectively improve both light harvesting and the charge carrier mobility by enhancing chain planarity and backbone linearity; the NDT copolymer has stronger noncovalent interactions and smaller bond angles than those of the BDT-based polymer. Moreover, the introduction of the NDT core brings about a drastic change in the molecular orientation into the face-on motif and results in polymer:PCBM blend films with well-mixed interpenetrating nanofibrillar bulk-heterojunction networks with small-scale phase separation, which produce solar cells with higher short-circuit current density and fill factor values. A conventional optimized device structure containing PNDT:PC71BM was found to exhibit a maximum solar efficiency of 6.35%, an open-circuit voltage of 0.84 V, a short-circuit current density of 11.92 mA cm(-2), and a fill factor of 63.5% with thermal annealing, which demonstrates that the NDT and DT2FBT moieties are a promising electron-donor/acceptor combination for high-performance photovoltaics.
Language	English
Publishing date	2015-09-30
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.5b04884
Database	MEDical Literature Analysis and Retrieval System OnLINE

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