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  1. Artikel ; Online: The addition of biochar and nitrogen alters the microbial community and their cooccurrence network by affecting soil properties.

    Yuan, Minshu / Zhu, Xiaozhen / Sun, Haoran / Song, Jingrong / Li, Chen / Shen, Yufang / Li, Shiqing

    Chemosphere

    2022  Band 312, Heft Pt 1, Seite(n) 137101

    Abstract: Biochar plays an important role in reducing the harmful environmental effects of inorganic nitrogen (N) fertilizers on agroecosystems, but the the impact mechanisms of biochar combined with N fertilizers on soil microorganisms are not clear enough. In ... ...

    Abstract Biochar plays an important role in reducing the harmful environmental effects of inorganic nitrogen (N) fertilizers on agroecosystems, but the the impact mechanisms of biochar combined with N fertilizers on soil microorganisms are not clear enough. In this study, high-throughput sequencing was used to study the influences of three N fertilizer levels (0 (N0), 90 (N90) and 120 (N120) kg ha
    Sprache Englisch
    Erscheinungsdatum 2022-11-02
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2022.137101
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  2. Artikel ; Online: Modelling future climate effects on N

    Song, Jingrong / Bao, Shushang / Bai, Ju / Dang, Yaai / Zeng, Xiang / Zhou, Jie / Shen, Yufang / Yue, Shanchao / Li, Shiqing

    Journal of environmental management

    2023  Band 351, Seite(n) 119854

    Abstract: Controlled-release urea application and straw incorporation have been conducted in recent years as environmental-friendly and sustainable farming strategies, but the long-term effects of controlled-release urea application and combination with straw on ... ...

    Abstract Controlled-release urea application and straw incorporation have been conducted in recent years as environmental-friendly and sustainable farming strategies, but the long-term effects of controlled-release urea application and combination with straw on the dryland maize yield, soil fertility and the environment under future climate scenarios remain unclear. Hence, based on a six-year field experiment, four treatments were used to calibrate and validate the DeNitrification-DeComposition (DNDC) model, including non-nitrogen (CK), split applications of conventional urea (UR), single basal application of conventional urea and controlled-release urea at a ratio of 2:1 (CU), and CU combined with straw (CUS). Subsequently, coupled the well-validated model with future climate to evaluate suitable agricultural production practices under two shared socioeconomic pathways (SSP)-SSP245 and SSP585. The validation results indicated a good fit between the simulated and observed data of greenhouse gas emissions, soil organic carbon (SOC) contents and maize yields. With the anticipation of warmer temperatures and increased precipitation in the future, the yields of UR, CU, and CUS treatment significantly rose. Under SSP585 scenario, the positive impacts of CU treatment on maize yields reduced after the 2050s, exhibiting an average decline of 12.03%. Compared with the UR treatment, the CU treatment markedly reduced cumulative N
    Mesh-Begriff(e) Soil ; Zea mays ; Carbon ; Greenhouse Gases ; Delayed-Action Preparations ; Nitrous Oxide/analysis ; Agriculture/methods ; Fertilizers/analysis ; China
    Chemische Substanzen Soil ; Carbon (7440-44-0) ; Greenhouse Gases ; Delayed-Action Preparations ; Nitrous Oxide (K50XQU1029) ; Fertilizers
    Sprache Englisch
    Erscheinungsdatum 2023-12-21
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 184882-3
    ISSN 1095-8630 ; 0301-4797
    ISSN (online) 1095-8630
    ISSN 0301-4797
    DOI 10.1016/j.jenvman.2023.119854
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: A novel iridoid glycoside leonuride (ajugol) attenuates airway inflammation and remodeling through inhibiting type-2 high cytokine/chemokine activity in OVA-induced asthmatic mice

    Yi, La / Zhou, Yaolong / Song, Jingrong / Tang, Weifeng / Yu, Hang / Huang, Xi / Shi, Hanlin / Chen, Mengmeng / Sun, Jing / Wei, Ying / Dong, Jingcheng

    Phytomedicine. 2022 July 17,

    2022  

    Abstract: Asthma is a chronic airway disorder with a hallmark feature of airflow obstruction that associated with the remodeling and inflammation in the airway wall. Effective therapy for controlling both remodeling and inflammation is still urgently needed. ... ...

    Abstract : Asthma is a chronic airway disorder with a hallmark feature of airflow obstruction that associated with the remodeling and inflammation in the airway wall. Effective therapy for controlling both remodeling and inflammation is still urgently needed. Leonuride is the main pharmacological component identified from Bu-Shen-Yi-Qi-Tang (BSYQT) which has been traditionally used in treatment of lung diseases. However, no pharmacological effects of leonuride in asthma were reported. : Here we aimed to investigated whether leonuride provided a therapeutic efficacy in reversing asthma airway remodeling and inflammation and uncover the underlying mechanisms. : Mouse models of chronic asthma were developed with ovalbumin (OVA) exposure for 8 weeks. Respiratory mechanics, lung histopathology and asthma-related cytokines were examined. Lung tissues were analyzed using RNA sequencing to reveal the transcriptional profiling changes. : After oral administration with leonuride (15mg/kg or 30mg/kg), mice exhibited a lower airway hyperresponsiveness in comparison to asthmatic mice. Leonuride suppressed airway inflammation evidenced by the significant reductions in accumulation of inflammatory cells around bronchi and vessels, leukocyte population counts and the abundance of type 2 inflammatory mediators (OVA specific IgE, IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF). On the other hand, leonuride slowed down the process of active remodeling as demonstrated by weaker goblet cell metaplasia and subepithelial fibrosis in lung histopathology and lower transforming growth factor (TGF)-β1 levels in serum and BALF in comparison to mice treated with OVA only. Furthermore, we uncovered transcriptional profiling alternations in lung tissue of mice after OVA exposure and leonuride treatment. Gene sets belonging to type-2 cytokine/chemokine activity stood out in leonuride target transcripts. Those upregulated (Bmp10, Ccl12, Ccl22, Ccl8, Ccl9, Cxcl15, Il13, Il33, Tnfrsf9, Il31ra, Il5ra, Il13ra2 and Ccl24) or downregulated (Acvr1c and Il18) genes in asthmatic mice, were all reversely regulated by leonuride treatment. : Our results revealed the therapeutic efficacy of leonuride in experimental chronic asthma for the first time, and implied that its anti-inflammatory and antifibrotic properties might be mediated by regulation of type-2 high cytokine/chemokines responses.
    Schlagwörter air flow ; asthma ; blood serum ; chemokines ; fibrosis ; genes ; histopathology ; inflammation ; interleukin-13 ; interleukin-18 ; interleukin-4 ; interleukin-5 ; iridoid glycosides ; leukocytes ; metaplasia ; mice ; oral administration ; ovalbumin ; transcription (genetics)
    Sprache Englisch
    Erscheinungsverlauf 2022-0717
    Erscheinungsort Elsevier GmbH
    Dokumenttyp Artikel
    Anmerkung Pre-press version
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2022.154345
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  4. Artikel ; Online: Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway

    Yu, Hang / Huang, Xi / Xie, Cong / Song, Jingrong / Zhou, Yaolong / Shi, Hanlin / Chen, Mengmeng / Wu, Yueren / Ruan, Zhenhui / Deng, Lingling / Deng, Xiaohong / Lv, Yubao / Luo, Qingli / Dong, Jingcheng

    Phytotherapy Research. 2023 Sept., v. 37, no. 9 p.4002-4017

    2023  

    Abstract: Persistent chronic inflammation of the lungs and airway remodeling are important pathological features that cannot be ignored in patients with chronic asthma. Apigenin (API) is a natural small molecule compound with good anti‐inflammatory and antioxidant ...

    Abstract Persistent chronic inflammation of the lungs and airway remodeling are important pathological features that cannot be ignored in patients with chronic asthma. Apigenin (API) is a natural small molecule compound with good anti‐inflammatory and antioxidant activity that has been widely reported in recent years, but its role in chronic asthma is not well defined. Our study began with oral gavage intervention using API (10, 20 mg/kg) or dexamethasone (DEX, 2 mg/kg) in a BALB/c mouse model of ovalbumin (OVA) sensitization. Different doses of API intervention effectively reduced airway resistance in the administered group. Additionally, inflammation was downregulated, mucus secretion was reduced, and airway remodeling was inhibited in the API intervention group compared with the model group. Asthma‐related inflammatory cytokines, such as IgE, IL‐4, IL‐5, IL‐13, and IL‐17, were downregulated in alveolar lavage fluid. Moreover, the apoptosis level of the administered group was found to be lower than that of the model group in the Tunel staining experiment. By analyzing transcriptome sequencing results, we found that API may exert anti‐inflammatory and anti‐apoptotic effects by inhibiting the MAPK pathway. Our subsequent results supported this conclusion, showing that the phosphorylation levels of ERKs, JNKs, and p38 MAPKs were inhibited in the administered group relative to the model group. Downstream expression of the apoptosis‐related protein B‐cell lymphoma‐2 (Bcl‐2) was upregulated, and the expression of Bcl‐2‐associated × protein (Bax) and cleaved caspase‐3 was downregulated. To further investigate the specific mechanism by which API acted, we established an in vitro model with house dust mite (HDM) stimulation, using API (10, 20 μM) for administration intervention. The results showed that API was able to improve cell viability, inhibit ROS production, and reverse HDM‐induced decreases in mitochondrial membrane potential (MMP) and apoptosis in airway epithelial cells via the MAPK pathway.
    Schlagwörter B-lymphocytes ; antioxidant activity ; apigenin ; apoptosis ; asthma ; caspase-3 ; cell viability ; dexamethasone ; dust mites ; epithelial cells ; epithelium ; inflammation ; interleukin-13 ; interleukin-17 ; interleukin-4 ; interleukin-5 ; membrane potential ; mice ; mitochondrial membrane ; models ; mucus ; ovalbumin ; phosphorylation ; phytotherapy ; secretion ; transcriptome ; transcriptomics
    Sprache Englisch
    Erscheinungsverlauf 2023-09
    Umfang p. 4002-4017.
    Erscheinungsort John Wiley & Sons, Ltd.
    Dokumenttyp Artikel ; Online
    Anmerkung JOURNAL ARTICLE
    ZDB-ID 639136-9
    ISSN 1099-1573 ; 0951-418X
    ISSN (online) 1099-1573
    ISSN 0951-418X
    DOI 10.1002/ptr.7859
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  5. Artikel ; Online: TMT-based quantitative proteomics revealed protective efficacy of Icariside II against airway inflammation and remodeling via inhibiting LAMP2, CTSD and CTSS expression in OVA-induced chronic asthma mice

    Zhou, Yaolong / Huang, Xi / Yu, Hang / Shi, Hanlin / Chen, Mengmeng / Song, Jingrong / Tang, Weifeng / Teng, Fangzhou / Li, Congcong / Yi, La / Zhu, Xueyi / Wang, Na / Wei, Ying / Wuniqiemu, Tulake / Dong, Jingcheng

    Phytomedicine. 2023 Sept., v. 118 p.154941-

    2023  

    Abstract: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, ...

    Abstract Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, underlying mechanisms with specifically targeted molecular expression by IS in asthma have not been fully understood, and whether IS could inhibit remodeling and EMT still remains unclear. The study aimed to clarify therapeutic efficacy of IS for attenuating airway inflammation and remodeling in asthma, and illustrate IS-regulated specific pathway and target proteins through TMT-based quantitative proteomics. Murine model of chronic asthma was constructed with ovalbumin (OVA) sensitization and then challenge for 8 weeks. Pulmonary function, leukocyte count in bronchoalveolar lavage fluid (BALF), lung histopathology, inflammatory and fibrotic cytokines, and markers of epithelial-mesenchymal transition (EMT) were evaluated. TMT-based quantitative proteomics were performed on lung tissues to explore IS-regulated proteins. IS contributed to alleviative airway hyperresponsiveness (AHR) evidenced by declined RL and increased Cdyn. After IS treatment, we observed a remarked down-regulation of leukocyte count, inflammatory cytokines in BALF, and peribronchial inflammation infiltration. Goblet cell hyperplasia, mucus secretion and peribronchial collagen deposition were attenuated, with the level of TGF-β and MMP-9 in BALF declined. Furthermore, IS induced a rise of Occludin and E-cadherin and a decline of N-cadherin and α-SMA in lung tissues. These results proved the protective property of IS against airway inflammation, remodeling and EMT. To further investigate underlying mechanisms of IS in asthma treatment, TMT-based quantitative proteomics were performed and 102 overlapped DEPs regulated by IS were identified. KEGG enrichment exhibited these DEPs were enriched in lysosome, phagosome and autophagy, in which LAMP2, CTSD and CTSS were common DEPs. WB, q-PCR and IHC results proofed expressional alteration of these proteins. Besides, IS could decrease Beclin-1 and LC3B expression with increasing p62 expression thus inhibiting autophagy. The study demonstrated IS could ameliorate AHR, airway inflammation, remodeling and EMT in OVA-induced chronic asthma mice. Our research was the first to reveal that inhibition of LAMP2, CTSD and CTSS expression in autophagy contributed to the therapeutic efficacy of IS to asthma.
    Schlagwörter air flow ; animal models ; anti-inflammatory activity ; asthma ; autophagy ; cadherins ; collagen ; cytokines ; herbal medicines ; histopathology ; hyperplasia ; inflammation ; leukocyte count ; lung function ; lungs ; lysosomes ; mucus ; occludins ; ovalbumin ; phagosomes ; proteomics ; secretion ; therapeutics ; Icariside II ; Quantitative proteomics ; Airway inflammation ; Airway remodeling ; EMT ; AHR ; AOD ; AOI ; BALF ; BSYQF ; Cdyn ; CTSD ; CTSS ; DEPs ; ECM ; EPO ; GO ; H&E ; IL ; IOD ; IS ; KEGG ; LAMP1 ; LAMP2 ; LC-MS ; MBP ; Mch ; MMP-9 ; NF-κB ; OVA ; PAS ; RL ; RP-HPLC ; TGF-β1 ; TMT
    Sprache Englisch
    Erscheinungsverlauf 2023-09
    Erscheinungsort Elsevier GmbH
    Dokumenttyp Artikel ; Online
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.154941
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  6. Artikel ; Online: Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway.

    Yu, Hang / Huang, Xi / Xie, Cong / Song, Jingrong / Zhou, Yaolong / Shi, Hanlin / Chen, Mengmeng / Wu, Yueren / Ruan, Zhenhui / Deng, Lingling / Deng, Xiaohong / Lv, Yubao / Luo, Qingli / Dong, Jingcheng

    Phytotherapy research : PTR

    2023  Band 37, Heft 9, Seite(n) 4002–4017

    Abstract: Persistent chronic inflammation of the lungs and airway remodeling are important pathological features that cannot be ignored in patients with chronic asthma. Apigenin (API) is a natural small molecule compound with good anti-inflammatory and antioxidant ...

    Abstract Persistent chronic inflammation of the lungs and airway remodeling are important pathological features that cannot be ignored in patients with chronic asthma. Apigenin (API) is a natural small molecule compound with good anti-inflammatory and antioxidant activity that has been widely reported in recent years, but its role in chronic asthma is not well defined. Our study began with oral gavage intervention using API (10, 20 mg/kg) or dexamethasone (DEX, 2 mg/kg) in a BALB/c mouse model of ovalbumin (OVA) sensitization. Different doses of API intervention effectively reduced airway resistance in the administered group. Additionally, inflammation was downregulated, mucus secretion was reduced, and airway remodeling was inhibited in the API intervention group compared with the model group. Asthma-related inflammatory cytokines, such as IgE, IL-4, IL-5, IL-13, and IL-17, were downregulated in alveolar lavage fluid. Moreover, the apoptosis level of the administered group was found to be lower than that of the model group in the Tunel staining experiment. By analyzing transcriptome sequencing results, we found that API may exert anti-inflammatory and anti-apoptotic effects by inhibiting the MAPK pathway. Our subsequent results supported this conclusion, showing that the phosphorylation levels of ERKs, JNKs, and p38 MAPKs were inhibited in the administered group relative to the model group. Downstream expression of the apoptosis-related protein B-cell lymphoma-2 (Bcl-2) was upregulated, and the expression of Bcl-2-associated × protein (Bax) and cleaved caspase-3 was downregulated. To further investigate the specific mechanism by which API acted, we established an in vitro model with house dust mite (HDM) stimulation, using API (10, 20 μM) for administration intervention. The results showed that API was able to improve cell viability, inhibit ROS production, and reverse HDM-induced decreases in mitochondrial membrane potential (MMP) and apoptosis in airway epithelial cells via the MAPK pathway.
    Mesh-Begriff(e) Animals ; Mice ; Apigenin/pharmacology ; Airway Remodeling ; Transcriptome ; Asthma/drug therapy ; Inflammation/metabolism ; Anti-Inflammatory Agents/pharmacology ; Apoptosis ; Epithelial Cells/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism
    Chemische Substanzen Apigenin (7V515PI7F6) ; Anti-Inflammatory Agents ; Proto-Oncogene Proteins c-bcl-2
    Sprache Englisch
    Erscheinungsdatum 2023-05-02
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 639136-9
    ISSN 1099-1573 ; 0951-418X
    ISSN (online) 1099-1573
    ISSN 0951-418X
    DOI 10.1002/ptr.7859
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: TMT-based quantitative proteomics revealed protective efficacy of Icariside II against airway inflammation and remodeling via inhibiting LAMP2, CTSD and CTSS expression in OVA-induced chronic asthma mice.

    Zhou, Yaolong / Huang, Xi / Yu, Hang / Shi, Hanlin / Chen, Mengmeng / Song, Jingrong / Tang, Weifeng / Teng, Fangzhou / Li, Congcong / Yi, La / Zhu, Xueyi / Wang, Na / Wei, Ying / Wuniqiemu, Tulake / Dong, Jingcheng

    Phytomedicine : international journal of phytotherapy and phytopharmacology

    2023  Band 118, Seite(n) 154941

    Abstract: Background: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory ... ...

    Abstract Background: Asthma is a chronic inflammatory disorder in airways with typical pathologic features of airflow limitation, airway inflammation and remodeling. Icariside II (IS), derived from herbal medicine Herba Epimedii, exerts an anti-inflammatory property. However, underlying mechanisms with specifically targeted molecular expression by IS in asthma have not been fully understood, and whether IS could inhibit remodeling and EMT still remains unclear.
    Purpose: The study aimed to clarify therapeutic efficacy of IS for attenuating airway inflammation and remodeling in asthma, and illustrate IS-regulated specific pathway and target proteins through TMT-based quantitative proteomics.
    Study design and methods: Murine model of chronic asthma was constructed with ovalbumin (OVA) sensitization and then challenge for 8 weeks. Pulmonary function, leukocyte count in bronchoalveolar lavage fluid (BALF), lung histopathology, inflammatory and fibrotic cytokines, and markers of epithelial-mesenchymal transition (EMT) were evaluated. TMT-based quantitative proteomics were performed on lung tissues to explore IS-regulated proteins.
    Results: IS contributed to alleviative airway hyperresponsiveness (AHR) evidenced by declined R
    Conclusions: The study demonstrated IS could ameliorate AHR, airway inflammation, remodeling and EMT in OVA-induced chronic asthma mice. Our research was the first to reveal that inhibition of LAMP2, CTSD and CTSS expression in autophagy contributed to the therapeutic efficacy of IS to asthma.
    Mesh-Begriff(e) Mice ; Animals ; Ovalbumin ; Proteomics ; Asthma/drug therapy ; Asthma/metabolism ; Lung/pathology ; Inflammation/metabolism ; Bronchoalveolar Lavage Fluid ; Cytokines ; Disease Models, Animal ; Mice, Inbred BALB C
    Chemische Substanzen Ovalbumin (9006-59-1) ; baohuoside I (113558-15-9) ; Cytokines
    Sprache Englisch
    Erscheinungsdatum 2023-06-25
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.154941
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: A novel iridoid glycoside leonuride (ajugol) attenuates airway inflammation and remodeling through inhibiting type-2 high cytokine/chemokine activity in OVA-induced asthmatic mice.

    Yi, La / Zhou, Yaolong / Song, Jingrong / Tang, Weifeng / Yu, Hang / Huang, Xi / Shi, Hanlin / Chen, Mengmeng / Sun, Jing / Wei, Ying / Dong, Jingcheng

    Phytomedicine : international journal of phytotherapy and phytopharmacology

    2022  Band 105, Seite(n) 154345

    Abstract: Background: Asthma is a chronic airway disorder with a hallmark feature of airflow obstruction that associated with the remodeling and inflammation in the airway wall. Effective therapy for controlling both remodeling and inflammation is still urgently ... ...

    Abstract Background: Asthma is a chronic airway disorder with a hallmark feature of airflow obstruction that associated with the remodeling and inflammation in the airway wall. Effective therapy for controlling both remodeling and inflammation is still urgently needed. Leonuride is the main pharmacological component identified from Bu-Shen-Yi-Qi-Tang (BSYQT) which has been traditionally used in treatment of lung diseases. However, no pharmacological effects of leonuride in asthma were reported.
    Purpose: Here we aimed to investigated whether leonuride provided a therapeutic efficacy in reversing asthma airway remodeling and inflammation and uncover the underlying mechanisms.
    Study design and methods: Mouse models of chronic asthma were developed with ovalbumin (OVA) exposure for 8 weeks. Respiratory mechanics, lung histopathology and asthma-related cytokines were examined. Lung tissues were analyzed using RNA sequencing to reveal the transcriptional profiling changes.
    Results: After oral administration with leonuride (15 mg/kg or 30 mg/kg), mice exhibited a lower airway hyperresponsiveness in comparison to asthmatic mice. Leonuride suppressed airway inflammation evidenced by the significant reductions in accumulation of inflammatory cells around bronchi and vessels, leukocyte population counts and the abundance of type 2 inflammatory mediators (OVA specific IgE, IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF). On the other hand, leonuride slowed down the process of active remodeling as demonstrated by weaker goblet cell metaplasia and subepithelial fibrosis in lung histopathology and lower transforming growth factor (TGF)-β1 levels in serum and BALF in comparison to mice treated with OVA only. Furthermore, we uncovered transcriptional profiling alternations in lung tissue of mice after OVA exposure and leonuride treatment. Gene sets belonging to type-2 cytokine/chemokine activity stood out in leonuride target transcripts. Those upregulated (Bmp10, Ccl12, Ccl22, Ccl8, Ccl9, Cxcl15, Il13, Il33, Tnfrsf9, Il31ra, Il5ra, Il13ra2 and Ccl24) or downregulated (Acvr1c and Il18) genes in asthmatic mice, were all reversely regulated by leonuride treatment.
    Conclusions: Our results revealed the therapeutic efficacy of leonuride in experimental chronic asthma for the first time, and implied that its anti-inflammatory and antifibrotic properties might be mediated by regulation of type-2 high cytokine/chemokines responses.
    Mesh-Begriff(e) Animals ; Asthma ; Bronchoalveolar Lavage Fluid ; Chemokines ; Cytokines ; Disease Models, Animal ; Inflammation ; Iridoid Glycosides ; Iridoids ; Lung ; Mice ; Mice, Inbred BALB C ; Ovalbumin ; Pyrans
    Chemische Substanzen Chemokines ; Cytokines ; Iridoid Glycosides ; Iridoids ; Pyrans ; Ovalbumin (9006-59-1) ; harpagide (OF59XHX7SR)
    Sprache Englisch
    Erscheinungsdatum 2022-07-22
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2022.154345
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Mycoplasma pneumoniae among Chinese Outpatient Children with Mild Respiratory Tract Infections during the Coronavirus Disease 2019 Pandemic.

    Chen, Jiande / Zhang, Jing / Lu, Zhiwei / Chen, Yu / Huang, Songsong / Li, Hengtao / Lin, Shuzhu / Yu, Jun / Zeng, Xueqi / Ji, Cuihong / Zheng, Yuejie / Dai, Fangfang / Dong, Wei / Xu, Huiting / Chen, Weichao / Jin, Xiaoqun / Cui, Zhen / Qiao, Jing / Qin, Wei /
    Chen, Hui / Jiang, Wei / Zhang, Xiaoying / Song, Jingrong / Shao, Jie / Su, Wen / Wang, Chao / Liu, Fang / Zhao, Yuhua / Zou, Yingxue / Guo, Run / Zhang, Lei / Wu, Jinhong / Yuan, Shuhua / Tang, Mingyu / Wu, Yufen / Lin, Jie / Dong, Wenfang / Chen, Xing / Sun, Xinrong / Yin, Yong

    Microbiology spectrum

    2022  Band 10, Heft 1, Seite(n) e0155021

    Abstract: Mycoplasma pneumoniae is a common pathogen causing respiratory disease in children. We sought to investigate the epidemiology of M. pneumoniae among outpatient children with mild respiratory tract infections (RTIs) during the coronavirus disease 2019 ( ... ...

    Abstract Mycoplasma pneumoniae is a common pathogen causing respiratory disease in children. We sought to investigate the epidemiology of M. pneumoniae among outpatient children with mild respiratory tract infections (RTIs) during the coronavirus disease 2019 (COVID-19) pandemic. Eligible patients were prospectively enrolled from January 2020 to June 2021. Throat swabs were tested for M. pneumoniae RNA. M. pneumoniae IgM was tested by a colloidal gold assay. Macrolide resistance and the effect of the COVID-19 countermeasures on M. pneumoniae prevalence were assessed. Symptom scores, treatments, and outcomes were evaluated. Eight hundred sixty-two eligible children at 15 centers in China were enrolled. M. pneumoniae was detected in 78 (9.0%) patients. Seasonally, M. pneumoniae peaked in the first spring and dropped dramatically to extremely low levels over time until the next summer. Decreases in COVID-19 prevalence were significantly associated with decreases in M. pneumoniae prevalence (
    Mesh-Begriff(e) Adolescent ; Adult ; Anti-Bacterial Agents/therapeutic use ; COVID-19/epidemiology ; Child ; Child, Preschool ; China/epidemiology ; Drug Resistance, Bacterial ; Female ; Humans ; Infant ; Macrolides/therapeutic use ; Male ; Mycoplasma pneumoniae/genetics ; Mycoplasma pneumoniae/isolation & purification ; Mycoplasma pneumoniae/physiology ; Outpatients/statistics & numerical data ; Pneumonia, Mycoplasma/drug therapy ; Pneumonia, Mycoplasma/epidemiology ; Pneumonia, Mycoplasma/microbiology ; Prospective Studies ; Respiratory Tract Infections/drug therapy ; Respiratory Tract Infections/epidemiology ; Respiratory Tract Infections/microbiology ; Young Adult
    Chemische Substanzen Anti-Bacterial Agents ; Macrolides
    Sprache Englisch
    Erscheinungsdatum 2022-02-09
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01550-21
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: [Molecular epidemiological and clinical features of coronavirus HKU1 in children with acute respiratory tract infection in Lanzhou].

    Song, Jing-rong / Jin, Yu / Duan, Zhao-jun / Yuan, Xin-hui / Yan, Kun-long / Zhao, Yang / Xie, Zhi-ping / Gao, Han-chun

    Zhonghua er ke za zhi = Chinese journal of pediatrics

    2010  Band 48, Heft 10, Seite(n) 744–747

    Abstract: Objective: Human coronavirus (CoV)-HKU1 (HCoV-HKU1) was first isolated by Woo et al in Hong Kong. Several successive reports confirmed retrospectively that this new human coronavirus was circulating in different countries worldwide. However, the impact ... ...

    Abstract Objective: Human coronavirus (CoV)-HKU1 (HCoV-HKU1) was first isolated by Woo et al in Hong Kong. Several successive reports confirmed retrospectively that this new human coronavirus was circulating in different countries worldwide. However, the impact and the role of the emerging HCoV-HKU1 were not defined in children with ARTI. The objective of this study was to investigate the molecular epidemiology and clinical characteristics of HCoV-HKU1 infection in children with ARTI in Lanzhou, China.
    Method: Nasopharyngeal aspiration (NPA) samples were collected from 301 children with ARTI at the First Hospital of Lanzhou University, Gansu Province, China, between November 2007 and October 2008. Demographic data and clinical findings of these children were collected at the same time. The informed consent was obtained from their parents. This study protocol was approved by the hospital ethics committee. The reverse transcription polymerase chain reaction (RT-PCR) was employed to screen HCoV-HKU1. Furthermore, other common respiratory viruses were screened in HCoV-HKU1 positive samples. All PCR positive products were sequenced, and phylogenetic analysis was conducted.
    Result: The overall frequency of HCoV-HKU1 infection was 5.0% (15/301). The HCoV-HKU1 pol gene sequences shared a 95.8% - 99.6% nucleotide identity with the human coronavirus-HKU1 strain, whereas the amino acid identity was 90.7% - 99.3%. The phylogenetic analysis revealed that the HCoV-HKU1 strain pol gene clustered with the HCoV-HKU1 strain N15 genotype B (no. DQ415911); 11 of 15 HCoV-HKU1 positive sample tested were mixed-infection. HCoV-HKU1 was detected only from November to April. Positive specimens peaked in November. Children with HCoV-HKU1 infection varied in age from 15 day to 12-years (median age, 10 months). The clinical diagnoses of HCoV-HKU1 positive patients included those with AURI and LURI. The clinical presentations of HCoV-HKU1 positive children included fever, cough, sputum production, diarrhea, vomiting; pharynx engorgement, crackles, and wheezing. The mean hospital stay of the 14 patients was 9.9 days. Six of 15 HCoV-HKU1 positive patients had an underlying illness, and they were all inpatients (hospital stay, mean, 11.2 days). There was no statistically significant difference in the detection rate between the two groups with and without underlying illnesses.
    Conclusion: Human CoV-HKU1 infection exists in children with respiratory tract infections in Lanzhou region. A single HCoV-HKU1 genotype B was circulating locally. The symptoms and clinical diagnoses of those infected with HCoV-HKU1 had no specificity as compared with patients with other common respiratory viruses infection.
    Mesh-Begriff(e) Child ; Child, Preschool ; China/epidemiology ; Coronavirus/classification ; Coronavirus/genetics ; Coronavirus/isolation & purification ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Molecular Epidemiology ; Respiratory Tract Infections/epidemiology ; Respiratory Tract Infections/virology
    Schlagwörter covid19
    Sprache Chinesisch
    Erscheinungsdatum 2010-10
    Erscheinungsland China
    Dokumenttyp English Abstract ; Journal Article
    ZDB-ID 784523-6
    ISSN 0578-1310
    ISSN 0578-1310
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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