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  1. Article: Determinants of Continuous Usage Intention in Community Group Buying Platform in China: Based on the Information System Success Model and the Expanded Technology Acceptance Model.

    Song, Yingjie / Gui, Lin / Wang, Hong / Yang, Yanru

    Behavioral sciences (Basel, Switzerland)

    2023  Volume 13, Issue 11

    Abstract: Community group buying is a new retail model with broad development prospects. The community group buying model in China has brought obvious social and economic benefits. However, the continuous usage rate on some community group buying platforms is ... ...

    Abstract Community group buying is a new retail model with broad development prospects. The community group buying model in China has brought obvious social and economic benefits. However, the continuous usage rate on some community group buying platforms is relatively low. Consumers' continuous usage intentions are closely related to the sustainable development of community group buying platforms. Therefore, this study integrates the information system success model (D&M model) and the expanded technology acceptance model (TAM) to construct a research model that explores the factors influencing consumers' continuous usage intentions from both the platform's and consumers' perspectives. The survey data involving 418 respondents who used community group buying platforms were developed and analyzed for structural equation model (SEM) testing. The results show the following: (1) Perceived usefulness, perceived ease of use, service quality, perceived trust, expectation confirmation, and subjective norms significantly affect continuous usage intention. (2) Subjective norms are significantly related to continuous usage intention. Perceived usefulness, perceived ease of use, service quality, perceived trust, and expectation confirmation indirectly affect continuous usage intention through user satisfaction. This research provides a new perspective for the theoretical research of community group buying and helps to promote the sustainable development of community group buying platforms in management practice.
    Language English
    Publishing date 2023-11-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2651997-5
    ISSN 2076-328X
    ISSN 2076-328X
    DOI 10.3390/bs13110941
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Comparative analysis of five type II TA systems identified in

    Song, Yingjie / Tang, Hong / Bao, Rui

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1127786

    Abstract: Background: Pseudomonas aeruginosa: Methods: We here investigated the diversity and function of five genomic type II TA systems widely distributed among : Results: ParDE, PA1030/PA1029, and HigBA could modulate persister cell formation under ... ...

    Abstract Background: Pseudomonas aeruginosa
    Methods: We here investigated the diversity and function of five genomic type II TA systems widely distributed among
    Results: ParDE, PA1030/PA1029, and HigBA could modulate persister cell formation under treatment with specific antibiotics. Furthermore, cell-based transcriptional and invasion assays revealed that PA1030/PA1029 and HigBA TA systems were critical for intracellular survival.
    Discussion: Our results highlight the prevalence and diverse roles of type II TA systems in
    MeSH term(s) Humans ; Pseudomonas aeruginosa/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Lung/metabolism ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Toxin-Antitoxin Systems/genetics ; Pseudomonas Infections/microbiology
    Chemical Substances Anti-Bacterial Agents ; Bacterial Toxins
    Language English
    Publishing date 2023-02-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1127786
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Pseudomonas aeruginosa two-component system CprRS regulates HigBA expression and bacterial cytotoxicity in response to LL-37 stress.

    Song, Yingjie / Zhang, Siping / Zhao, Ninglin / Nong, Cheng / He, Yongxing / Bao, Rui

    PLoS pathogens

    2024  Volume 20, Issue 1, Page(s) e1011946

    Abstract: Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained ... ...

    Abstract Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained largely elusive. In this study, we investigate the two-component system CprRS in P. aeruginosa and unveil the crucial role of the sensor protein CprS in sensing the human host defense peptide LL-37, thereby modulating bacterial virulence. We demonstrate that CprS acts as a phosphatase in the presence of LL-37, leading to the phosphorylation and activation of the response regulator CprR. The results prove that CprR directly recognizes a specific sequence within the promoter region of the HigBA toxin-antitoxin system, resulting in enhanced expression of the toxin HigB. Importantly, LL-37-induced HigB expression promotes the production of type III secretion system effectors, leading to reduced expression of proinflammatory cytokines and increased cytotoxicity towards macrophages. Moreover, mutations in cprS or cprR significantly impair bacterial survival in both macrophage and insect infection models. This study uncovers the regulatory mechanism of the CprRS system, enabling P. aeruginosa to detect and respond to human innate immune responses while maintaining a balanced virulence gene expression profile. Additionally, this study provides new evidence and insights into the complex regulatory system of T3SS in P. aeruginosa within the host environment, contributing to a better understanding of host-microbe communication and the development of novel strategies to combat bacterial infections.
    MeSH term(s) Humans ; Pseudomonas aeruginosa/metabolism ; Pseudomonas Infections/genetics ; Pseudomonas Infections/microbiology ; Virulence Factors/genetics ; Virulence Factors/metabolism ; Virulence ; Type III Secretion Systems/metabolism ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial
    Chemical Substances Virulence Factors ; Type III Secretion Systems ; Bacterial Proteins
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1011946
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  4. Article ; Online: Molecular mechanism of siderophore regulation by the Pseudomonas aeruginosa BfmRS two-component system in response to osmotic stress.

    Song, Yingjie / Wu, Xiyu / Li, Ze / Ma, Qin Qin / Bao, Rui

    Communications biology

    2024  Volume 7, Issue 1, Page(s) 295

    Abstract: Pseudomonas aeruginosa, a common nosocomial pathogen, relies on siderophores to acquire iron, crucial for its survival in various environments and during host infections. However, understanding the molecular mechanisms of siderophore regulation remains ... ...

    Abstract Pseudomonas aeruginosa, a common nosocomial pathogen, relies on siderophores to acquire iron, crucial for its survival in various environments and during host infections. However, understanding the molecular mechanisms of siderophore regulation remains incomplete. In this study, we found that the BfmRS two-component system, previously associated with biofilm formation and quorum sensing, is essential for siderophore regulation under high osmolality stress. Activated BfmR directly bound to the promoter regions of pvd, fpv, and femARI gene clusters, thereby activating their transcription and promoting siderophore production. Subsequent proteomic and phenotypic analyses confirmed that deletion of BfmRS reduces siderophore-related proteins and impairs bacterial survival in iron-deficient conditions. Furthermore, phylogenetic analysis demonstrated the high conservation of the BfmRS system across Pseudomonas species, functional evidences also indicated that BfmR homologues from Pseudomonas putida KT2440 and Pseudomonas sp. MRSN12121 could bind to the promoter regions of key siderophore genes and osmolality-mediated increases in siderophore production were observed. This work illuminates a novel signaling pathway for siderophore regulation and enhances our understanding of siderophore-mediated bacterial interactions and community establishment.
    MeSH term(s) Humans ; Siderophores/metabolism ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism ; Osmotic Pressure ; Phylogeny ; Proteomics ; Iron/metabolism ; Pseudomonas/metabolism ; Pseudomonas Infections
    Chemical Substances Siderophores ; Iron (E1UOL152H7)
    Language English
    Publishing date 2024-03-09
    Publishing country England
    Document type Journal Article
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-024-05995-z
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  5. Article: Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view

    Song, Yingjie / Ke, Yitao / Kang, Mei / Bao, Rui

    Computational and Structural Biotechnology Journal. 2022, v. 20

    2022  

    Abstract: Members of the high temperature requirement A (HtrA) protein family are widely distributed amongst prokaryotic and eukaryotic species. HtrA proteins haveATP-independent dualchaperone-proteaseactivity and mediate protein quality control. Emerging evidence ...

    Abstract Members of the high temperature requirement A (HtrA) protein family are widely distributed amongst prokaryotic and eukaryotic species. HtrA proteins haveATP-independent dualchaperone-proteaseactivity and mediate protein quality control. Emerging evidence indicates that HtrA family members arevitalfor establishing infections and bacterial survival under stress conditions. Bacterial HtrA proteins are increasingly thought of as important new targets for antibacterial drug development.Recent literature suggeststhat HtrA protein AlgW from Pseudomonas aeruginosa has distinctstructural,functional, and regulatorycharacteristics. The novel dual-signal activation mechanism seen in AlgW is required to modulate stress and drug responses in bacteria, prompting us to review our understanding of the many HtrA proteins found in microorganisms. Here, we describe the distribution of HtrA gene orthologues in pathogenic bacteria, discuss theirstructure–functionrelationships, outline the molecular mechanisms exhibited by different bacterial HtrA proteins inbacteriaunder selective pressure, and review thesignificance of recently developed small molecule inhibitors targetingHtrA in pathogenic bacteria.
    Keywords Pseudomonas aeruginosa ; antibiotics ; biotechnology ; genes ; protein value ; quality control ; temperature ; therapeutics
    Language English
    Size p. 40-49.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2694435-2
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2021.12.004
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Different Phenylalanine Pathway Responses to Cold Stress Based on Metabolomics and Transcriptomics in Tartary Buckwheat Landraces

    Song, Yingjie / Feng, Jinchao / Liu, Dongmei / Long, Chunlin

    Journal of agricultural and food chemistry. 2022 Jan. 06, v. 70, no. 2

    2022  

    Abstract: Tartary buckwheat (Fagopyrum tataricum) is strongly adapted to growth in adverse environments. In Liangshan, the Yi people cultivate different Tartary buckwheat landraces in different habitats. In this study, we aimed to understand the molecular ... ...

    Abstract Tartary buckwheat (Fagopyrum tataricum) is strongly adapted to growth in adverse environments. In Liangshan, the Yi people cultivate different Tartary buckwheat landraces in different habitats. In this study, we aimed to understand the molecular differences in transcriptomic and metabolomic responses underlying cold tolerance between two Tartary buckwheat landraces (TM and RG) cultivated at different altitudes. After cold treatment, TM showed normal growth in the seedling stage and had significantly higher total flavonoids (16.53 mg/g, 1.47 times), rutin (5.73 mg/g, 1.32 times), and quercetin (0.08 mg/g, 2.67 times), which were higher than those in RG. In addition, TM showed higher-level changes in carbon and nitrogen metabolism than RG. Combined transcriptome and metabolomic analyses showed that phenylpropanoid biosynthesis was upregulated after cold treatment, and in TM, rutin synthesis was upregulated with a higher-level response to cold stress. RG showed higher expression in anthocyanins in response to cold stress. In addition, 24 structural genes involved in flavonoid synthesis, including 6 PAL, 3 C4H, 2 4CL, 2 CHS, 1 CHI, 3 F3H, 3 DFR, 1 FLS, 1 F3′H, and 4 GTR genes, were identified. These results will provide sufficient information for breeding Tartary buckwheat with high cold tolerance and constructing rutin high-yield varieties based on genetic engineering.
    Keywords Fagopyrum tataricum ; anthocyanins ; biosynthesis ; buckwheat ; carbon ; cold stress ; cold tolerance ; cold treatment ; food chemistry ; landraces ; metabolomics ; nitrogen metabolism ; people ; phenylalanine ; quercetin ; rutin ; seedlings ; transcriptome ; transcriptomics
    Language English
    Dates of publication 2022-0106
    Size p. 687-698.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.1c06915
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    In stock of ZB MED Bonn / Germany

    Z 1185: Show issues

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  7. Article ; Online: Efficient Proton Conductor Based on Bismuth Oxide Clusters and Polyoxometalates.

    Song, Yingjie / Fang, Qing / Liu, Bailing / Hu, Bo / Su, Zhongmin

    Langmuir : the ACS journal of surfaces and colloids

    2023  Volume 39, Issue 41, Page(s) 14511–14518

    Abstract: Developing new solid-state electrolyte materials for improving the proton conductivity remains an important challenge. Herein, a novel two-dimensional layered solid-state proton conductor ... ...

    Abstract Developing new solid-state electrolyte materials for improving the proton conductivity remains an important challenge. Herein, a novel two-dimensional layered solid-state proton conductor Bi
    Language English
    Publishing date 2023-10-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/acs.langmuir.3c01441
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  8. Article ; Online: Different Phenylalanine Pathway Responses to Cold Stress Based on Metabolomics and Transcriptomics in Tartary Buckwheat Landraces.

    Song, Yingjie / Feng, Jinchao / Liu, Dongmei / Long, Chunlin

    Journal of agricultural and food chemistry

    2022  Volume 70, Issue 2, Page(s) 687–698

    Abstract: Tartary buckwheat ( ...

    Abstract Tartary buckwheat (
    MeSH term(s) Anthocyanins ; Cold-Shock Response ; Fagopyrum/genetics ; Fagopyrum/metabolism ; Humans ; Metabolomics ; Phenylalanine ; Plant Breeding ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Transcriptome
    Chemical Substances Anthocyanins ; Plant Proteins ; Phenylalanine (47E5O17Y3R)
    Language English
    Publishing date 2022-01-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.1c06915
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation

    Yang, Lan / Li, Hao / Han, Yao / Song, Yingjie / Wei, Mingchen / Fang, Mengya / Sun, Yansong

    Genes (Basel). 2023 Mar. 27, v. 14, no. 4

    2023  

    Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host ...

    Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein—γH2AX—was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system.
    Keywords CRISPR-Cas systems ; DNA damage ; cell growth ; gene expression ; gene ontology ; genes ; transcriptome
    Language English
    Dates of publication 2023-0327
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14040806
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  10. Article ; Online: CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation.

    Yang, Lan / Li, Hao / Han, Yao / Song, Yingjie / Wei, Mingchen / Fang, Mengya / Sun, Yansong

    Genes

    2023  Volume 14, Issue 4

    Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host ...

    Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein-γH2AX-was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system.
    MeSH term(s) Gene Editing ; CRISPR-Cas Systems/genetics ; Genome ; DNA Damage/genetics ; Gene Expression
    Language English
    Publishing date 2023-03-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14040806
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