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  1. Article ; Online: Probiotic Supplementation for Treatment of Infection

    Na Rae Lim / Soo Yeon Choi / Woo Chul Chung

    The Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol 23, Iss 1, Pp 34-

    A Double-Blind Randomized Clinical Trial

    2023  Volume 41

    Abstract: Background/Aims The effects of probiotic supplementation on Helicobacter pylori (H. pylori) eradication therapy are not completely understood. In this study, we investigated the effects of continuous probiotic administration on eradication rates, ... ...

    Abstract Background/Aims The effects of probiotic supplementation on Helicobacter pylori (H. pylori) eradication therapy are not completely understood. In this study, we investigated the effects of continuous probiotic administration on eradication rates, recrudescence, and symptom response following completion of a course of H. pylori therapy. Methods This prospective, randomized, double-blind placebo-controlled trial was performed between June 2018 and 2020. Twohundred seventy patients who received a standard triple regimen for H. pylori eradication, were included in the study. Participants were randomized to receive a probiotic as adjunctive therapy (Enterococcus faecium 4.5×108 and Bacillus subtilis 5.0×107; Medilac-S®, Hanmi Pharmaceuticals, Seoul, Korea) or a placebo (one tablet thrice daily) for 28 days, following H. pylori eradication. Participants who showed successful eradication underwent a repeat 13C-urea breath test after 6 months. Results Eradication rates in the probiotic and placebo groups were 77.1% and 72.4%, respectively (P=0.48) using per-protocol analysis. Using intention-to-treat analysis, eradication rates were 67.4% and 65.9%, respectively (P=0.43). Of 149 patients who were followed-up after 6 months, four patients had recrudescence (2.7%). Recrudescence rates did not differ between the probiotic and placebo groups. Of the 76 patients who had non-ulcer dyspepsia, 60 (78.9%) showed symptom resolution after 6 months. This beneficial effect was most pronounced in patients with postprandial distress syndrome (P=0.02). Conclusions Consecutive probiotic supplementation following H. pylori eradication therapy did not increase eradication rates or decrease recrudescence rates.
    Keywords disease eradication ; probiotics ; Internal medicine ; RC31-1245
    Subject code 610 ; 150
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Yong Chan Lee
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Tailored Therapy Using Bismuth Add-on Standard Triple Therapy vs. Concomitant Therapy

    Soo Yeon Choi / Na Rae Lim / Woo Chul Chung

    The Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol 23, Iss 2, Pp 118-

    A First-line Regimen for Helicobacter pylori Infection

    2023  Volume 124

    Abstract: Background/Aims Compared with other regimens, concomitant therapy (CT) used as a first-line regimen for Helicobacter pylori (H. pylori) infection is associated with higher eradication rates. We compared the efficacy of tailored therapy (TT) using bismuth ...

    Abstract Background/Aims Compared with other regimens, concomitant therapy (CT) used as a first-line regimen for Helicobacter pylori (H. pylori) infection is associated with higher eradication rates. We compared the efficacy of tailored therapy (TT) using bismuth added to standard triple therapy (STT) with CT. Methods This consecutive study performed between September 2020 and 2021 included 210 patients with H. pylori infection. Two participating gastroenterologists prescribed TT and CT. Multiplex PCR assays were performed before eradication therapy to identify the relevant point mutations and confirm clarithromycin resistance in the TT group (n=105). Patients who showed negative PCR results received 14-day STT and those with positive PCR results received a 14-day regimen of bismuth added to STT. The other group (n=105) received 10-day CT. Results Based on per-protocol analysis, eradication rates in the TT and CT groups were 89.2% (91/102) and 81.6% (84/103), respectively. We observed no statistically significant intergroup differences in eradication rates (P=0.12). The frequency of estimated clarithromycin resistance confirmed using multiplex PCR assays was 32.4% (34/105), and the eradication rate associated with bismuth add-on STT was 76.5% (26/34) in patients with clarithromycin resistance. Conclusions Considering the current and emerging trends in antibiotic resistance, a therapeutic strategy using TT (bismuth add-on STT) is recommended to minimize unnecessary administration of antibiotics.
    Keywords clarithromycin ; eradication ; Internal medicine ; RC31-1245
    Subject code 616
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Yong Chan Lee
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Severe hypoglycemia as a preventable risk factor for cardiovascular disease in patients with type 2 diabetes mellitus

    Soo-Yeon Choi / Seung-Hyun Ko

    The Korean Journal of Internal Medicine, Vol 36, Iss 2, Pp 263-

    2021  Volume 270

    Abstract: Glucose-lowering medication and lifestyle modification are essential for optimal glycemic control in patients with type 2 diabetes mellitus (T2DM). However, glucose-lowering agents, particularly insulin and insulin secretagogues, may cause hypoglycemia, ... ...

    Abstract Glucose-lowering medication and lifestyle modification are essential for optimal glycemic control in patients with type 2 diabetes mellitus (T2DM). However, glucose-lowering agents, particularly insulin and insulin secretagogues, may cause hypoglycemia, which has multiple negative effects on the cardiovascular (CV) system and may cause death. Previous studies using institutional data from the Korean Nationwide Health Insurance database have consistently found a causal relationship between severe hypoglycemia and CV outcomes and mortality. Screening for high-risk patients, appropriate management, and intensive individualized education are the most effective measures and essential for the prevention of harmful hypoglycemic events. Based on identified risk factors that predict severe hypoglycemia, we developed an 1-year risk prediction model for severe hypoglycemia that can be used in clinical settings. In this review, we describe the current understanding of severe hypoglycemia and the clinical implications in patients with T2DM. Furthermore, we highlight the importance of intensive individualized education for high-risk patients and the risk prediction model to reduce severe hypoglycemia.
    Keywords diabetes mellitus ; type 2 ; severe hypoglycemia ; cardiovascular diseases ; education ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher The Korean Association of Internal Medicine
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: First Report of Bacterial Wilt by Ralstonia pseudosolanacearum on Peanut in Korea

    Soo Yeon Choi / Nam Goo Kim / Sang-Min Kim / Bong Choon Lee

    Research in Plant Disease, Vol 28, Iss 1, Pp 54-

    2022  Volume 56

    Abstract: A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected ... ...

    Abstract A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected sample on TZC media. The dominated colony on media was isolated colony on media was isolated and subcultured of purification. The pure cultured bacteria was identified as Ralstonia solanacearum by sequencing of 16S rRNA gene. Multiplex polymerase chain reaction using phylotype-specific primer set identified isolate as phylotype I (R. pseudosolanacearum). Phylogenetic tree was constructed based on 16S rRNA sequence and it was closed with R. pseudosolanacearum. Pathogenicity of the isolates was assessed by soil drenching inoculation on 4-week-old peanut plant. The wilt symptom was successfully reproduced by inoculation of the isolates after 14 days. This is first report of bacterial wilt caused by R. pseudosolanacearum on peanut in Korea.
    Keywords bacterial wilt ; peanut ; ralstonia pseudosolanacearum ; Agriculture (General) ; S1-972
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Hanrimwon Publishing Company
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Evolution and Phylogeny of Soybean Mosaic Virus Based on 143 Complete Genomes

    Hoseong Choi / Yeonhwa Jo / Soo Yeon Choi / Sang-Min Kim / Yu Mi Choi / Jin-Sung Hong / Bong Choon Lee / Won Kyong Cho

    International Journal of Molecular Sciences, Vol 24, Iss 1, p

    2022  Volume 22

    Abstract: Soybean mosaic virus (SMV) of the genus Potyvirus is an important virus in cultivated soybeans. Here, we obtained 7 SMV genomes from soybean germplasms using RNA sequencing and conducted a comprehensive evolutionary and phylogenetic study of 143 SMV ... ...

    Abstract Soybean mosaic virus (SMV) of the genus Potyvirus is an important virus in cultivated soybeans. Here, we obtained 7 SMV genomes from soybean germplasms using RNA sequencing and conducted a comprehensive evolutionary and phylogenetic study of 143 SMV genomes derived from 10 plant species and 12 countries. The phylogenetic tree we constructed using coding DNA sequences revealed the existence of nine clades of SMV isolates/strains. Recombination analysis revealed 76 recombinant events and 141 recombinants in total. Clades 1 and 3 contain the most common SMV pathotypes, including G1 through G7, which are distributed worldwide. Clade 2 includes several Chinese SMV pathotypes. The SMV isolates were further divided into two groups. The SMV isolates in the first group, including clades 8 and 9, were identified from Pinellia and Atractylodes species, whereas those in the second group (clades 1 through 7) were mostly found in cultivated soybeans. The SMV polyprotein undergoes positive selection, whereas most mature proteins, except for the P1 protein, undergo negative selection. The P1 protein of SMV isolates in group 1 may be highly correlated with host adaptation. This study provides strong evidence that recombination and plant hosts are powerful forces driving the genetic diversity of the SMV genome.
    Keywords soybean mosaic virus ; genome ; evolution ; phylogeny ; diversity ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification

    Shinhwa Kim / Bong Choon Lee / Hyun Ju Kim / Soo Yeon Choi / Su Jwa Seo / Sang Min Kim

    Research in Plant Disease, Vol 26, Iss 4, Pp 195-

    2020  Volume 201

    Abstract: Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) ... ...

    Abstract Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis.
    Keywords bacterial leaf blight ; detection ; recombinase polymerase amplification ; xanthomonas oryzae pv. oryzae ; Agriculture (General) ; S1-972
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Hanrimwon Publishing Company
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Assessment of water quality variation of a monitoring network using exploratory factor analysis and empirical orthogonal function

    Kim, Sung Eun / Il Won Seo / Soo Yeon Choi

    Environmental modelling & software. 2017 Aug., v. 94

    2017  

    Abstract: This study suggests a systematic assessment method that jointly uses the exploratory factor analysis (EFA) and empirical orthogonal function (EOF-patterns) of Principal Component Analysis (PCA) to assess the water quality variation of the monitoring ... ...

    Abstract This study suggests a systematic assessment method that jointly uses the exploratory factor analysis (EFA) and empirical orthogonal function (EOF-patterns) of Principal Component Analysis (PCA) to assess the water quality variation of the monitoring network of Nakdong River, Korea, in which 28 stations measuring 15 water quality parameters are located. The EFA results showed the monitoring stations to be distinguished by two main factors. The representative stations of which the variance was almost explained by the specific factor were selected. We applied PCA to the monitoring data of representative stations, and then analyzed the EOF-patterns that indicate the characteristics of water-quality variation for each factor. With the interpretation of main factors and EOF-patterns causing dominant water quality variations, the monitoring network of Nakdong River could be spatially and seasonally evaluated according to the contribution of each factor.
    Keywords computer software ; environmental models ; factor analysis ; monitoring ; principal component analysis ; rivers ; variance ; water quality ; Korean Peninsula
    Language English
    Dates of publication 2017-08
    Size p. 21-35.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 1364-8152
    DOI 10.1016/j.envsoft.2017.03.035
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Loss of glutamate dehydrogenase in Ralstonia solanacearum alters dehydrogenase activity, extracellular polysaccharide production and bacterial virulence

    Wu, Jing / Eun Joo Jung / Eunsook Chung / Hyoung Ju Lee / Hyun Gi Kong / Seon-Woo Lee / Soo Yeon Choi / Weixin Tao

    Physiological and molecular plant pathology. 2015 Apr., v. 90

    2015  

    Abstract: Metabolism in Ralstonia solanacearum, which causes lethal wilt on Solanaceous plants, is poorly understood. In this study, we selected a Tn5-inserted mutant of R. solanacearum SL341 showing various phenotypic changes and altered virulence. When the gdhA ...

    Abstract Metabolism in Ralstonia solanacearum, which causes lethal wilt on Solanaceous plants, is poorly understood. In this study, we selected a Tn5-inserted mutant of R. solanacearum SL341 showing various phenotypic changes and altered virulence. When the gdhA gene encoding NAD(P)+-dependent glutamate dehydrogenase was disrupted, the gdhA mutant of SL341 (SL341P2) was defective in red colony development on tetrazolium chloride-amended medium and showed less extracellular polysaccharide (EPS) production. The growth rate of the gdhA mutant on rich medium did not differ from that of the wild-type strain; however, its growth on minimal medium with glutamate as the sole carbon source was completely inhibited. SL341P2 was also defective in the oxidation of several carbon sources compared to the wild type. All the observed defects of SL341P2 gdhA mutant were fully or partially restored by providing the gdhA gene in trans. The gdhA mutant showed reduced virulence after soil-soaking inoculation of tomato plants, both on susceptible tomato cultivar Moneymaker and on the well-known bacterial-wilt-resistant cultivar Hawaii 7996. The delayed disease development by the gdhA mutant was due to slower multiplication of the mutant bacteria than wild type in tomato plants. Taken together, these results indicate that GdhA is required for diverse metabolic functions in R. solanacearum, including normal production of the virulence factor EPS, as well as normal bacterial growth in planta and full virulence on tomato plants.
    Keywords bacteria ; carbon ; cultivars ; genes ; glutamate dehydrogenase ; glutamic acid ; metabolism ; microbial growth ; mutants ; NAD (coenzyme) ; oxidation ; phenotype ; polysaccharides ; Ralstonia solanacearum ; Solanum lycopersicum var. lycopersicum ; tetrazolium ; tomatoes ; virulence
    Language English
    Dates of publication 2015-04
    Size p. 57-64.
    Publishing place Elsevier India Pvt Ltd.
    Document type Article
    ZDB-ID 742470-x
    ISSN 0885-5765
    ISSN 0885-5765
    DOI 10.1016/j.pmpp.2015.03.003
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Resistance Evaluation of Tomato Germplasm against Bacterial Wilt by Ralstonia solanacearum

    Eun Joo Jung / Hae Jin Joo / Soo Yeon Choi / Seung Yeup Lee / Yong Hoon Jung / Myung Hwan Lee / Hyun Gi Kong / Seon-Woo Lee

    Research in Plant Disease, Vol 20, Iss 4, Pp 253-

    2014  Volume 258

    Abstract: This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato ... ...

    Abstract This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneymaker were used as a resistant control plant and a susceptible control plant, respectively. A total of 32 cultivars were susceptible and 10 cultivars showed various disease response suggesting resistant phenotype segregation in the lines. Five commercial cultivars and 5 breeding lines exhibited strong resistance to bacterial wilt by the SL341 strain. These 5 breeding lines might be used for further study of plant defense response against bacterial wilt and cloning of the resistance gene from tomato plants. Ultimately, the selected lines could be used for tomato breeding to generate bacterial wilt resistant tomato plants
    Keywords Bacterial wilt ; Resistance evaluation ; Solanum lycopersicum ; Agriculture (General) ; S1-972
    Language English
    Publishing date 2014-12-01T00:00:00Z
    Publisher Hanrimwon Publishing Company
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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